ABSTRACT
A rapid and highly sensitive flow-injection chemiluminometric method was developed for determination of 3 sympathomimetic drugs, namely, etilefrine hydrochloride, isoxsuprine hydrochloride, and prenalterol hydrochloride. The method is based on chemiluminescence induced by oxidation of drugs with acidified potassium permanganate in the presence of formic acid as a carrier. The calibration graphs were linear over the concentration ranges 0.2-9, 0.2-12.5, and 0.025-1.25 microg/mL for the 3 compounds, respectively. The method was applied successfully in determining the drugs in dosage forms and in biological fluids. A proposal for the reaction pathway is suggested.
Subject(s)
Phenols/analysis , Sympathomimetics/analysis , Calibration , Etilefrine/analysis , Etilefrine/blood , Etilefrine/urine , Flow Injection Analysis , Indicators and Reagents , Isoxsuprine/analysis , Isoxsuprine/blood , Isoxsuprine/urine , Luminescent Measurements , Phenols/blood , Phenols/urine , Potassium Permanganate , Prenalterol/analysis , Prenalterol/blood , Prenalterol/urine , Sympathomimetics/blood , Sympathomimetics/urine , TabletsABSTRACT
A simple and highly sensitive fluorimetric method was developed for the routine determination of prenalterol hydrochloride in bulk, in dosage forms and in biological fluids. The method is based on the fluorescence induced by reaction of the nitroso-derivative of prenalterol hydrochloride with 2-cyanoacetamide in the presence of ammonia. The different experimental parameters were carefully studied and incorporated into the procedure. The fluorescence is measured at 440 nm after excitation at 368 nm. Fluorescence intensity is a linear function of prenalterol hydrochloride concentration over the range of 0.1-2.8 microg x ml(-1) in the solution finally measured. A proposal for the reaction pathway was suggested.
Subject(s)
Adrenergic beta-Agonists/analysis , Prenalterol/analysis , Adrenergic beta-Agonists/blood , Adrenergic beta-Agonists/urine , Ammonia , Fluorescent Dyes , Humans , Indicators and Reagents , Nitriles , Nitroso Compounds , Pharmaceutical Solutions , Prenalterol/blood , Prenalterol/urine , Spectrometry, Fluorescence , TabletsABSTRACT
A rapid and sensitive fluorimetric method for the determination of prenalterol hydrochloride is presented, based on the oxidation of the analyte with potassium hexacyanoferrate(III) in a slightly alkaline medium (pH 9.23). The different experimental parameters were carefully studied and incorporated into the procedure. The oxidation product exhibits a blue fluorescence with its emission maximum at 427 nm, and excitation maximum at 314 nm. Fluorescence intensity is a linear function of prenalterol hydrochloride concentration over the range of 0.2-3.6 microg/ml(-1) in the solution finally measured. The method was successfully applied to the determination of prenalterol hydrochloride in pharmaceutical formulations and biological fluids. A proposal for the reaction pathway is suggested.
Subject(s)
Fluorometry/methods , Prenalterol/analysis , Body Fluids/chemistry , Calibration , Ferrocyanides , Humans , Prenalterol/blood , Prenalterol/urine , Sympathomimetics/analysis , Sympathomimetics/blood , Sympathomimetics/urineABSTRACT
A simple and sensitive polarographic method is described for the determination of prenalterol hydrochloride through treatment with nitrous acid. The different experimental parameters affecting the derivatization process, as well as the polarographic analysis were studied. The derivatization product was found to be reducible at the dropping mercury electrode over the whole pH range in Britton-Robinson buffers. At pH 5, a well defined diffusion-controlled cathodic wave was produced. The limiting current vs. the concentration plot was linear over the range 0.015-0.15 mM in the direct current mode, with a minimum detectability of 0.8 microM. The proposed method was applied successfully to the determination of prenalterol hydrochloride either in the pure form or in its dosage forms.
