ABSTRACT
We have developed a quick, highly sensitive immunoassay method for drugs by latex agglutination inhibition. An antiserum against primidone (PRM) was obtained by immunizing rabbits with PRM-bovine serum albumin conjugate. PRM-rabbit serum albumin conjugate sensitized latex was agglutinated with diluted antiserum, and the agglutination was inhibited by free PRM quantitatively. Turbidity of the agglutination suspension was measured by spectrophotometry as absorbance. Larger latex gave higher sensitivity than the smaller, because its agglutination was inhibited more intensely by free PRM. The assay values of this method were correlated well with those obtained by an enzyme immunoassay method.
Subject(s)
Latex Fixation Tests , Nephelometry and Turbidimetry , Primidone/analysis , Animals , Immunoenzyme Techniques , Particle Size , Polystyrenes , Primidone/immunology , Rabbits , Reproducibility of Results , Serum Albumin, Bovine , Spectrophotometry, UltravioletABSTRACT
A short discussion of known facts on iatrogenic autoallergic dermatoses of the pemphigus and lupus erythematosus type followed by the clinical, histological and immunological data of a subepidermal autoallergic bullous dermatosis in two children after anticonvulsive treatment with primidon.
