Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Language
Publication year range
1.
Mol Biol Rep ; 47(8): 5889-5901, 2020 Aug.
Article in English | MEDLINE | ID: mdl-32661871

ABSTRACT

H2O2 generated during the oxidative burst, plays important roles in plant defenses responses against pathogens. In this study we examined the role of H2O2 on bacterial canker resistance in transgenic plums over-expressing cytosolic superoxide dismutase. Three transgenic lines (C64, C66 and F12) with elevated levels of H2O2 accumulation showed enhanced resistance against bacterial canker disease caused by Pseudomonas syringae pv. syringae, when compared to the non-transformed control. Analysis of the expression of several genes involved in the plant-pathogen interaction showed that the expression of those involved in SA pathway (pr1 and npr1) and JA (lox3) were activated earlier and transiently in transgenic lines C66 and F12 when compared to the wild type. However, the expression of genes involved in anthocyanin synthesis (chi, chs, f3h, dfr, atcs, myb10) and ethylene (acs) was induced at very low levels whereas it was activated by the pathogen at exaggerated levels in the non-transformed line. These results suggest that resistance observed in transgenic lines over-producing H2O2 is correlated with an early and transient induction of defense genes associated with the SA and JA pathways and inhibition of gene expression associated with ethylene and anthocyanin biosynthesis.


Subject(s)
Hydrogen Peroxide/metabolism , Plant Diseases/immunology , Prunus domestica/metabolism , Pseudomonas syringae , Superoxide Dismutase/biosynthesis , Superoxide Dismutase/genetics , Cytosol/enzymology , Disease Resistance , Oxidants/metabolism , Plant Diseases/microbiology , Plants, Genetically Modified , Prunus domestica/genetics , Prunus domestica/immunology , Prunus domestica/microbiology , Superoxide Dismutase/metabolism
2.
Electron. j. biotechnol ; 30: 103-109, nov. 2017. ilus, tab, graf
Article in English | LILACS | ID: biblio-1021917

ABSTRACT

Background: Small ribonucleic acids represent an important repertoire of mobile molecules that exert key roles in several cell processes including antiviral defense. Small RNA based repertoire includes both small interfering RNA (siRNA) and microRNA (miRNA) molecules. In the Prunus genus, sharka disease, caused by the Plum pox virus (PPV), first occurred on European plum (Prunus domestica) and then spread over among all species in this genus and thus classified as quarantine pathogen. Next-generation sequencing (NGS) was used for the study of siRNA/miRNA molecules; however, NGS relies on adequate extraction protocols. Currently, knowledge of PPV-Prunus interactions in terms of siRNA populations and miRNA species is still scarce, and siRNA/miRNA extraction protocols are limited to species such as peach, almond, and sweet cherry. Results: We describe a reliable procedure for siRNA/miRNA purification from Prunus salicina trees, in which previously used protocols did not allow adequate purification. The procedure was based on a combination of commercially available RNA purification kits and specific steps that yielded high quality purifications. The resulting molecules were adequate for library construction and NGS, leading to the development of a pipeline for analysis of both siRNAs and miRNAs in the PPV­P. salicina interactions. Results showed that PPV infection led to altered siRNA profiles in Japanese plum as characterized by decreased 24-nt and increased 21- and 22-nt siRNAs. Infections showed miR164 and miR160 generation and increased miR166, miR171, miR168, miR319, miR157, and miR159. Conclusion: We propose this protocol as a reliable and reproducible small RNA isolation procedure for P. salicina and other Prunus species.


Subject(s)
RNA, Plant/isolation & purification , MicroRNAs/isolation & purification , RNA, Small Interfering/isolation & purification , Prunus domestica/genetics , Plant Diseases/virology , Plum Pox Virus/physiology , Host-Pathogen Interactions , High-Throughput Nucleotide Sequencing , Real-Time Polymerase Chain Reaction , Prunus domestica/immunology , Prunus domestica/virology
3.
PLoS One ; 10(12): e0144670, 2015.
Article in English | MEDLINE | ID: mdl-26658051

ABSTRACT

RNA-Seq has proven to be a very powerful tool in the analysis of the Plum pox virus (PPV, sharka disease)/Prunus interaction. This technique is an important complementary tool to other means of studying genomics. In this work an analysis of gene expression of resistance/susceptibility to PPV in apricot is performed. RNA-Seq has been applied to analyse the gene expression changes induced by PPV infection in leaves from two full-sib apricot genotypes, "Rojo Pasión" and "Z506-7", resistant and susceptible to PPV, respectively. Transcriptomic analyses revealed the existence of more than 2,000 genes related to the pathogen response and resistance to PPV in apricot. These results showed that the response to infection by the virus in the susceptible genotype is associated with an induction of genes involved in pathogen resistance such as the allene oxide synthase, S-adenosylmethionine synthetase 2 and the major MLP-like protein 423. Over-expression of the Dicer protein 2a may indicate the suppression of a gene silencing mechanism of the plant by PPV HCPro and P1 PPV proteins. On the other hand, there were 164 genes involved in resistance mechanisms that have been identified in apricot, 49 of which are located in the PPVres region (scaffold 1 positions from 8,050,804 to 8,244,925), which is responsible for PPV resistance in apricot. Among these genes in apricot there are several MATH domain-containing genes, although other genes inside (Pleiotropic drug resistance 9 gene) or outside (CAP, Cysteine-rich secretory proteins, Antigen 5 and Pathogenesis-related 1 protein; and LEA, Late embryogenesis abundant protein) PPVres region could also be involved in the resistance.


Subject(s)
Gene Expression Regulation, Plant/immunology , Genes, Plant , Host-Pathogen Interactions/genetics , Plant Diseases/genetics , Plum Pox Virus/physiology , Prunus armeniaca/genetics , Prunus domestica/genetics , ATP-Binding Cassette Transporters/genetics , ATP-Binding Cassette Transporters/immunology , Disease Susceptibility , Genetic Pleiotropy , Genotype , Host-Pathogen Interactions/immunology , Intramolecular Oxidoreductases/genetics , Intramolecular Oxidoreductases/immunology , Membrane Glycoproteins/genetics , Membrane Glycoproteins/immunology , Methionine Adenosyltransferase/genetics , Methionine Adenosyltransferase/immunology , Molecular Sequence Annotation , Plant Diseases/immunology , Plant Diseases/virology , Plant Immunity/genetics , Plant Leaves/genetics , Plant Leaves/immunology , Plant Leaves/virology , Plant Proteins/genetics , Plant Proteins/immunology , Plum Pox Virus/pathogenicity , Prunus armeniaca/immunology , Prunus armeniaca/virology , Prunus domestica/immunology , Prunus domestica/virology , Ribonuclease III/genetics , Ribonuclease III/immunology , Transcriptome/immunology
SELECTION OF CITATIONS
SEARCH DETAIL
...