ABSTRACT
The study of prokaryotic life in high temperature environments viz., geothermal areas, hot, acidic geysers and undersea hydrothermal vents has revealed the existence of thermophiles (or hyperthermophiles). These microorganisms possess various stress adaptation mechanisms which enable them to bypass multiple physical and chemical barriers for survival. The discovery of radiation resistant thermophile Deinococcus geothermalis has given new insights into the field of radiation microbiology. The ability of radiation resistant thermophiles to deal with the lethal effects of ionizing radiations like DNA damage, oxidative bursts and protein damage has made them a model system for exobiology and interplanetary transmission of life. They might be an antiquity of historical transport process that brought microbial life on Earth. These radiation resistant thermophiles are resistant to desiccation as well and maintain their homeostasis by advance DNA repair mechanisms, reactive oxygen species (ROS) detoxification system and accumulation of compatible solutes. Moreover, engineered radioresistant thermophilic strains are the best candidate for bioremediation of radionuclide waste while the extremolytes produced by these organisms may have predicted therapeutic uses. So, the present article delineate a picture of radiation resistance thermophiles, their adaptive mechanisms to evade stress viz., radiation and desiccation, their present applications along with new horizons in near future.
Subject(s)
Archaea/physiology , Archaea/radiation effects , Bacteria/radiation effects , Bacterial Physiological Phenomena/radiation effects , Hot Temperature , Actinobacteria/physiology , Actinobacteria/radiation effects , Bacteria/genetics , Bacterial Physiological Phenomena/genetics , Biodegradation, Environmental , Cyanobacteria/physiology , Cyanobacteria/radiation effects , DNA Damage/radiation effects , DNA Repair , Deinococcus/genetics , Deinococcus/physiology , Deinococcus/radiation effects , Environmental Microbiology , Exobiology , Halobacterium/physiology , Halobacterium/radiation effects , Pyrococcus/physiology , Pyrococcus/radiation effects , Radiation, Ionizing , Reactive Oxygen Species/radiation effects , Respiratory Burst/radiation effects , Stress, Physiological , Sulfolobus/physiology , Sulfolobus/radiation effects , Thermococcus/physiology , Thermococcus/radiation effectsABSTRACT
The mechanisms by which hyperthermophilic Archaea, such as "Pyrococcus abyssi" and Pyrococcus furiosus, survive high doses of ionizing gamma irradiation are not thoroughly elucidated. Following gamma-ray irradiation at 2,500 Gy, the restoration of "P. abyssi" chromosomes took place within chromosome fragmentation. DNA synthesis in irradiated "P. abyssi" cells during the DNA repair phase was inhibited in comparison to nonirradiated control cultures, suggesting that DNA damage causes a replication block in this organism. We also found evidence for transient export of damaged DNA out of irradiated "P. abyssi" cells prior to a restart of chromosomal DNA synthesis. Our cell fractionation assays further suggest that "P. abyssi" contains a highly efficient DNA repair system which is continuously ready to repair the DNA damage caused by high temperature and/or ionizing radiation.
Subject(s)
DNA Damage , DNA Repair , Gamma Rays/adverse effects , Hot Temperature , Pyrococcus/physiology , Blotting, Western , Culture Media , DNA Replication , Pyrococcus/growth & development , Pyrococcus/radiation effects , Pyrococcus furiosus/physiology , Pyrococcus furiosus/radiation effects , Radiation, IonizingABSTRACT
Hyperthermophilic archaea of the genus Pyrococcus are resistant to gamma radiation, suggesting that efficient mechanisms for DNA repair exist in these organisms. To determine whether protective mechanisms might also be implicated in this radioresistance, we have estimated the linear density of DNA double-stranded breaks caused by gamma irradiation in the genomic DNA of two Pyrococcus species, using Escherichia coli and the radioresistant bacterium Deinococcus radiodurans as controls. The linear density of double-stranded breaks was essentially the same in all four microorganisms when irradiation was carried under similar anaerobic conditions, indicating that no specific DNA protection mechanisms exist in Pyrococcus species. Using one- and two-dimensional gel electrophoresis we compared the protein patterns from Pyrococcus abyssi and P. furiosus cells that had or had not been exposed to gamma rays. We did not detect any significant protein induction following DNA damage in either species.
