Subject(s)
Anisomycin , Antifungal Agents , Ascomycota/metabolism , Pyrrolidines , Anisomycin/analogs & derivatives , Anisomycin/biosynthesis , Anisomycin/pharmacology , Antifungal Agents/biosynthesis , Antifungal Agents/pharmacology , Candida/drug effects , Chromatography, Thin Layer , Fermentation , Fungi/drug effects , Isomerism , Magnetic Resonance Spectroscopy , Molecular Conformation , Molecular Structure , Pyrrolidines/analogs & derivatives , Pyrrolidines/biosynthesis , Pyrrolidines/pharmacologyABSTRACT
A new antibiotic, reductiomycin was isolated from the culture broth of Streptomyces griseorubiginosus. The antibiotic has the molecular formula C14H15O6N(M.W. 293). Reductiomycin is active against Gram-positive bacteria, fungi, and newcastle disease virus.
Subject(s)
Anti-Bacterial Agents/biosynthesis , Pyrrolidines/biosynthesis , Bacteria/drug effects , Fermentation , Fungi/drug effects , Newcastle disease virus/drug effects , Pyrrolidines/isolation & purification , Pyrrolidines/pharmacology , Soil Microbiology , Streptomyces/analysis , Streptomyces/classification , Streptomyces/metabolismSubject(s)
Aminoglycosides/biosynthesis , Anti-Bacterial Agents/biosynthesis , Dactinomycin/biosynthesis , Streptomycetaceae/metabolism , Chemical Phenomena , Chemistry , Erythromycin/biosynthesis , Gentamicins/biosynthesis , Glycosides/biosynthesis , Lactams, Macrocyclic , Leucomycins/biosynthesis , Neomycin/biosynthesis , Pyrrolidines/biosynthesis , Rifamycins/biosynthesis , Sisomicin/analogs & derivatives , Sisomicin/biosynthesisSubject(s)
Anti-Bacterial Agents/biosynthesis , Streptomyces/metabolism , Adsorption , Aminosalicylic Acids/metabolism , Anti-Bacterial Agents/analysis , Anti-Bacterial Agents/pharmacology , Chromatography, Paper , Chromatography, Thin Layer , Countercurrent Distribution , Fermentation , Filtration , Ion Exchange Resins , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Pyrrolidines/analysis , Pyrrolidines/biosynthesis , Pyrrolidines/pharmacology , Spectrophotometry, Infrared , Thioglycosides/analysis , Thioglycosides/biosynthesis , Thioglycosides/pharmacologyABSTRACT
The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess Delta(1)-pyrroline-5-carboxylate, the immediate precursor of proline, is added to a culture. In proline auxotrophs blocked at an early step in proline biosynthesis (proA or proB), reversion to prototrophy is often due to a mutation in the arginine pathway which diverts N-acetyl glutamate gamma-semialdehyde to proline synthesis, thus bypassing the proA or proB block. In such double mutants (proAB, argD), the last step in proline synthesis appears to be unregulated, since proline is excreted. Feedback inhibition and repression of the arginine pathway overcomes indirect suppression (restoring the Pro(-) phenotype), but proline regulation is not restored; double mutants still excrete proline when fed Delta(1)-pyrroline-5-carboxylate exogeneously. A new class of proline analogue-resistant mutant, due to mutation at argD, is also described.