ABSTRACT
Acinetobacter baumannii is considered as a major cause of nosocomial infection worldwide. Various vaccine formulations have been mostly studied based on secreted or surface-exposed proteins of A. baumannii in murine models. Serum resistance proteins are critical virulence factors in bloodstream infections. AbOmpA and PKF are two major factors involved in serum resistance and could be considered as promising vaccine targets. In this study IgG1, IgG2c, Total-IgG concentrations, survival rates and spleen bacterial loads were studied in C57/BL mice model according to PKF, AbOmpA and AbOmpA + PKF vaccine formulations. The findings showed significant raises of IgG2c and Total-IgG in all three vaccinated groups in comparison with the control group. Whereas, there were low concentrations of IgG1 in all immunization plans. Colony counts of mice spleen showed the bacterial load of PKF plan had the most decrease of bacterial load (DBL = 5 log10â¯CFU/g). Taken together, this evaluation indicated that PKF vaccination plan induced a polarized Th1 response and rendered an effective protection against bloodstream infection caused by A. baumannii.
Subject(s)
Acinetobacter Infections/immunology , Acinetobacter Infections/prevention & control , Acinetobacter baumannii/pathogenicity , Antibody Formation/immunology , Bacterial Outer Membrane Proteins/immunology , R Factors/blood , Sepsis/microbiology , Acinetobacter Infections/microbiology , Acinetobacter baumannii/genetics , Animals , Bacterial Load , Bacterial Outer Membrane Proteins/genetics , Bacterial Proteins/genetics , Bacterial Proteins/immunology , Bacterial Vaccines/immunology , Cloning, Molecular , Disease Models, Animal , Genes, Bacterial/genetics , Immunization , Immunoglobulin G/blood , Mice , Mice, Inbred C57BL , R Factors/immunology , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Spleen/immunology , Survival Rate , Vaccines, Synthetic/genetics , Vaccines, Synthetic/immunology , Virulence Factors/genetics , Virulence Factors/immunologyABSTRACT
En este trabajo exponemos los datos experimentales sobre el estudio de algunos factores físico-químicos que influyen sobre la resistencia globular osmótica. Queda demostrado que las concentraciones elevadas de hidrogeniones provocan aumento de la hemolisis y que en medio alcalino los eritrocitos son más resistentes a este proceso. Además que el aumento de la temperatura hasta 50 oC disminuye la reistencia osmótica de los glóbulos rojos humanos
Subject(s)
Humans , Erythrocytes/chemistry , Hemolysis , R Factors/blood , Body Temperature , Hydrogen-Ion ConcentrationABSTRACT
En este trabajo exponemos los datos experimentales sobre el estudio de algunos factores físico-químicos que influyen sobre la resistencia globular osmótica. Queda demostrado que las concentraciones elevadas de hidrogeniones provocan aumento de la hemolisis y que en medio alcalino los eritrocitos son mas resistentes a este proceso. Ademas que el aumento de la temperatura hasta 50 oC disminuye la reistencia osmótica de los glóbulos rojos humanos (AU)
