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1.
Nucleic Acids Res ; 52(7): 3924-3937, 2024 Apr 24.
Article in English | MEDLINE | ID: mdl-38421610

ABSTRACT

RNA ligases are important enzymes in molecular biology and are highly useful for the manipulation and analysis of nucleic acids, including adapter ligation in next-generation sequencing of microRNAs. Thermophilic RNA ligases belonging to the RNA ligase 3 family are gaining attention for their use in molecular biology, for example a thermophilic RNA ligase from Methanobacterium thermoautotrophicum is commercially available for the adenylation of nucleic acids. Here we extensively characterise a newly identified RNA ligase from the thermophilic archaeon Palaeococcus pacificus (PpaRnl). PpaRnl exhibited significant substrate adenylation activity but low ligation activity across a range of oligonucleotide substrates. Mutation of Lys92 in motif I to alanine, resulted in an enzyme that lacked adenylation activity, but demonstrated improved ligation activity with pre-adenylated substrates (ATP-independent ligation). Subsequent structural characterisation revealed that in this mutant enzyme Lys238 was found in two alternate positions for coordination of the phosphate tail of ATP. In contrast mutation of Lys238 in motif V to glycine via structure-guided engineering enhanced ATP-dependent ligation activity via an arginine residue compensating for the absence of Lys238. Ligation activity for both mutations was higher than the wild-type, with activity observed across a range of oligonucleotide substrates with varying sequence and secondary structure.


Subject(s)
RNA Ligase (ATP) , RNA Ligase (ATP)/metabolism , RNA Ligase (ATP)/genetics , RNA Ligase (ATP)/chemistry , Substrate Specificity , Archaeal Proteins/metabolism , Archaeal Proteins/genetics , Archaeal Proteins/chemistry , Planococcaceae/enzymology , Planococcaceae/genetics , Protein Engineering , Mutation , Models, Molecular , Adenosine Triphosphate/metabolism , Oligonucleotides/metabolism , Oligonucleotides/genetics
2.
Cell Mol Life Sci ; 80(12): 352, 2023 Nov 07.
Article in English | MEDLINE | ID: mdl-37935993

ABSTRACT

To be functional, some RNAs require a processing step involving splicing events. Each splicing event necessitates an RNA ligation step. RNA ligation is a process that can be achieved with various intermediaries such as self-catalysing RNAs, 5'-3' and 3'-5' RNA ligases. While several types of RNA ligation mechanisms occur in human, RtcB is the only 3'-5' RNA ligase identified in human cells to date. RtcB RNA ligation activity is well known to be essential for the splicing of XBP1, an essential transcription factor of the unfolded protein response; as well as for the maturation of specific intron-containing tRNAs. As such, RtcB is a core factor in protein synthesis and homeostasis. Taking advantage of the high homology between RtcB orthologues in archaea, bacteria and eukaryotes, this review will provide an introduction to the structure of RtcB and the mechanism of 3'-5' RNA ligation. This analysis is followed by a description of the mechanisms regulating RtcB activity and localisation, its known partners and its various functions from bacteria to human with a specific focus on human cancer.


Subject(s)
RNA Ligase (ATP) , Transcription Factors , Humans , RNA Ligase (ATP)/genetics , RNA Ligase (ATP)/chemistry , RNA Ligase (ATP)/metabolism , Transcription Factors/metabolism , RNA/metabolism , Unfolded Protein Response , RNA, Transfer/genetics , RNA, Transfer/metabolism , RNA Splicing/genetics
3.
Elife ; 102021 12 02.
Article in English | MEDLINE | ID: mdl-34854379

ABSTRACT

RtcB enzymes are RNA ligases that play essential roles in tRNA splicing, unfolded protein response, and RNA repair. In metazoa, RtcB functions as part of a five-subunit tRNA ligase complex (tRNA-LC) along with Ddx1, Cgi-99, Fam98B, and Ashwin. The human tRNA-LC or its individual subunits have been implicated in additional cellular processes including microRNA maturation, viral replication, DNA double-strand break repair, and mRNA transport. Here, we present a biochemical analysis of the inter-subunit interactions within the human tRNA-LC along with crystal structures of the catalytic subunit RTCB and the N-terminal domain of CGI-99. We show that the core of the human tRNA-LC is assembled from RTCB and the C-terminal alpha-helical regions of DDX1, CGI-99, and FAM98B, all of which are required for complex integrity. The N-terminal domain of CGI-99 displays structural homology to calponin-homology domains, and CGI-99 and FAM98B associate via their N-terminal domains to form a stable subcomplex. The crystal structure of GMP-bound RTCB reveals divalent metal coordination geometry in the active site, providing insights into its catalytic mechanism. Collectively, these findings shed light on the molecular architecture and mechanism of the human tRNA ligase complex and provide a structural framework for understanding its functions in cellular RNA metabolism.


Subject(s)
Proteins/chemistry , RNA Ligase (ATP)/chemistry , Trans-Activators/chemistry , Humans , Molecular Structure
4.
RNA Biol ; 18(sup2): 623-639, 2021 11 12.
Article in English | MEDLINE | ID: mdl-34766865

ABSTRACT

Despite the development of non-radioactive DNA/RNA labelling methods, radiolabelled nucleic acids are commonly used in studies focused on the determination of RNA fate. Nucleic acid fragments with radioactive nucleotide analoguesincorporated into the body or at the 5' or 3' terminus of the molecule can serve as probes in hybridization-based analyses of in vivo degradation and processing of transcripts. Radiolabelled oligoribonucleotides are utilized as substrates in biochemical assays of various RNA metabolic enzymes, such as exo- and endoribonucleases, nucleotidyltransferases or helicases. In some applications, the placement of the label is not a concern, while in other cases it is required that the radioactive mark is located at the 5'- or 3'-end of the molecule. An unsurpassed method for 5'-end RNA labelling employs T4 polynucleotide kinase (PNK) and [γ-32P]ATP. In the case of 3'-end labelling, several different possibilities exist. However, they require the use of costly radionucleotide analogues. Previously, we characterized an untypical nucleotidyltransferase named CutA, which preferentially incorporates cytidines at the 3'-end of RNA substrates. Here, we demonstrate that this unusual feature can be used for the development of a novel, efficient, reproducible and economical method of RNA 3'-end labelling by CutA-mediated cytidine tailing. The labelling efficiency is comparable to that achieved with the most common method applied to date, i.e. [5'-32P]pCp ligation to the RNA 3'-terminus catalysed by T4 RNA ligase I. We show the utility of RNA substrates labelled using our new method in exemplary biochemical assays assessing directionality of two well-known eukaryotic exoribonucleases, namely Dis3 and Xrn1.


Subject(s)
Nucleotidyltransferases/chemistry , RNA/chemistry , Staining and Labeling/methods , Cytidine Triphosphate/chemistry , In Vitro Techniques , Isotope Labeling/methods , Nucleotides/chemistry , Phosphorus Radioisotopes , RNA/genetics , RNA Ligase (ATP)/chemistry , Staining and Labeling/standards , Substrate Specificity , Uridine Triphosphate/chemistry
5.
J Am Chem Soc ; 143(21): 8154-8163, 2021 06 02.
Article in English | MEDLINE | ID: mdl-34028252

ABSTRACT

Threose nucleic acid (TNA) has been considered a potential RNA progenitor in evolution due to its chemical simplicity and base pairing property. Catalytic TNA sequences with RNA ligase activities might have facilitated the transition to the RNA world. Here we report the isolation of RNA ligase TNA enzymes by in vitro selection. The identified TNA enzyme T8-6 catalyzes the formation of a 2'-5' phosphoester bond between a 2',3'-diol and a 5'-triphosphate group, with a kobs of 1.1 × 10-2 min-1 (40 mM Mg2+, pH 9.0). For efficient reaction, T8-6 requires UA|GA at the ligation junction and tolerates variations at other substrate positions. Functional RNAs such as hammerhead ribozyme can be prepared by T8-6-catalyzed ligation, with site-specific introduction of a 2'-5' linkage. Together, this work provides experimental support for TNA as a plausible pre-RNA genetic polymer and also offers an alternative molecular tool for biotechnology.


Subject(s)
Nucleic Acids/metabolism , RNA Ligase (ATP)/metabolism , Tetroses/metabolism , Nucleic Acid Conformation , Nucleic Acids/chemistry , RNA Ligase (ATP)/chemistry , Tetroses/chemistry
6.
Mol Cell ; 81(12): 2520-2532.e16, 2021 06 17.
Article in English | MEDLINE | ID: mdl-33930333

ABSTRACT

The tRNA ligase complex (tRNA-LC) splices precursor tRNAs (pre-tRNA), and Xbp1-mRNA during the unfolded protein response (UPR). In aerobic conditions, a cysteine residue bound to two metal ions in its ancient, catalytic subunit RTCB could make the tRNA-LC susceptible to oxidative inactivation. Here, we confirm this hypothesis and reveal a co-evolutionary association between the tRNA-LC and PYROXD1, a conserved and essential oxidoreductase. We reveal that PYROXD1 preserves the activity of the mammalian tRNA-LC in pre-tRNA splicing and UPR. PYROXD1 binds the tRNA-LC in the presence of NAD(P)H and converts RTCB-bound NAD(P)H into NAD(P)+, a typical oxidative co-enzyme. However, NAD(P)+ here acts as an antioxidant and protects the tRNA-LC from oxidative inactivation, which is dependent on copper ions. Genetic variants of PYROXD1 that cause human myopathies only partially support tRNA-LC activity. Thus, we establish the tRNA-LC as an oxidation-sensitive metalloenzyme, safeguarded by the flavoprotein PYROXD1 through an unexpected redox mechanism.


Subject(s)
Oxidoreductases Acting on Sulfur Group Donors/metabolism , RNA Ligase (ATP)/metabolism , RNA, Transfer/metabolism , Animals , Antioxidants/physiology , Catalytic Domain , Female , HeLa Cells , Humans , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , NAD/metabolism , NADP/metabolism , Oxidation-Reduction , Oxidoreductases/metabolism , Oxidoreductases Acting on Sulfur Group Donors/physiology , RNA Ligase (ATP)/chemistry , RNA Ligase (ATP)/genetics , RNA Splicing/genetics , RNA Splicing/physiology , Unfolded Protein Response/physiology , X-Box Binding Protein 1/metabolism
7.
Nucleic Acids Res ; 48(10): 5603-5615, 2020 06 04.
Article in English | MEDLINE | ID: mdl-32315072

ABSTRACT

Naegleria gruberi RNA ligase (NgrRnl) exemplifies the Rnl5 family of adenosine triphosphate (ATP)-dependent polynucleotide ligases that seal 3'-OH RNA strands in the context of 3'-OH/5'-PO4 nicked duplexes. Like all classic ligases, NgrRnl forms a covalent lysyl-AMP intermediate. A two-metal mechanism of lysine adenylylation was established via a crystal structure of the NgrRnl•ATP•(Mn2+)2 Michaelis complex. Here we conducted an alanine scan of active site constituents that engage the ATP phosphates and the metal cofactors. We then determined crystal structures of ligase-defective NgrRnl-Ala mutants in complexes with ATP/Mn2+. The unexpected findings were that mutations K170A, E227A, K326A and R149A (none of which impacted overall enzyme structure) triggered adverse secondary changes in the active site entailing dislocations of the ATP phosphates, altered contacts to ATP, and variations in the numbers and positions of the metal ions that perverted the active sites into off-pathway states incompatible with lysine adenylylation. Each alanine mutation elicited a distinctive off-pathway distortion of the ligase active site. Our results illuminate a surprising plasticity of the ligase active site in its interactions with ATP and metals. More broadly, they underscore a valuable caveat when interpreting mutational data in the course of enzyme structure-function studies.


Subject(s)
Alanine , Amino Acid Substitution , Lysine/chemistry , RNA Ligase (ATP)/chemistry , RNA Ligase (ATP)/genetics , Adenosine Monophosphate/chemistry , Adenosine Triphosphate/chemistry , Catalytic Domain , Lysine/metabolism , Manganese/chemistry , Models, Molecular , Naegleria/enzymology , RNA Ligase (ATP)/metabolism
8.
Int J Biochem Cell Biol ; 122: 105744, 2020 05.
Article in English | MEDLINE | ID: mdl-32234548

ABSTRACT

The human archease, hereafter named HArch, is identified as a key cofactor of the tRNA-splicing ligase complex, and a potential therapeutic target for treating nervous system injuries. However, little is known about the structural basis of HArch in tRNA maturation, mRNA splicing, and RNA repair. Here we report the crystal structures of HArch and its two mutants D51A and D178A with resolutions ranging from 1.96 Å to 3.4 Å. HArch is composed of an extended N-terminal protrusion domain (NTD) and one compacted C-terminal domain (CTD). Unlike previously reported homologous proteins, the NTD of the first subunit interacts with the CTD of the second one, and this interaction might be important for maintaining protein stability. Moreover, HArch interacts and colocalizes with RNA ligase RTCB in cells. Our current study reveals the atomic structure of HArch and may help us understand its function in mRNA splicing.


Subject(s)
RNA Ligase (ATP)/chemistry , RNA-Binding Proteins/chemistry , Crystallography, X-Ray , Humans , RNA Ligase (ATP)/metabolism , RNA-Binding Proteins/metabolism , Transfection
9.
Proc Natl Acad Sci U S A ; 117(11): 5741-5748, 2020 03 17.
Article in English | MEDLINE | ID: mdl-32123094

ABSTRACT

The hypothesized central role of RNA in the origin of life suggests that RNA propagation predated the advent of complex protein enzymes. A critical step of RNA replication is the template-directed synthesis of a complementary strand. Two experimental approaches have been extensively explored in the pursuit of demonstrating protein-free RNA synthesis: template-directed nonenzymatic RNA polymerization using intrinsically reactive monomers and ribozyme-catalyzed polymerization using more stable substrates such as biological 5'-triphosphates. Despite significant progress in both approaches in recent years, the assembly and copying of functional RNA sequences under prebiotic conditions remains a challenge. Here, we explore an alternative approach to RNA-templated RNA copying that combines ribozyme catalysis with RNA substrates activated with a prebiotically plausible leaving group, 2-aminoimidazole (2AI). We applied in vitro selection to identify ligase ribozymes that catalyze phosphodiester bond formation between a template-bound primer and a phosphor-imidazolide-activated oligomer. Sequencing revealed the progressive enrichment of 10 abundant sequences from a random sequence pool. Ligase activity was detected in all 10 RNA sequences; all required activation of the ligator with 2AI and generated a 3'-5' phosphodiester bond. We propose that ribozyme catalysis of phosphodiester bond formation using intrinsically reactive RNA substrates, such as imidazolides, could have been an evolutionary step connecting purely nonenzymatic to ribozyme-catalyzed RNA template copying during the origin of life.


Subject(s)
Imidazoles/chemistry , Origin of Life , RNA Ligase (ATP)/chemistry , RNA, Catalytic/chemistry , Imidazoles/metabolism , Polymerization , RNA Ligase (ATP)/metabolism , RNA, Catalytic/metabolism
10.
Talanta ; 212: 120754, 2020 May 15.
Article in English | MEDLINE | ID: mdl-32113533

ABSTRACT

Robust, reliable, and sensitively quantitative detection of genetic biomarkers at single-base resolution has the potential to revolutionize medical diagnostics, especially for precision medicine. Here, taking the advantages of the high specificity of ligase reaction and the powerful amplification features of the isothermally exponential amplification, we have demonstrated a novel methodology to sensitively quantify genetic biomarkers at one-base resolution. The methodology is based on the ligase reaction of two stem-loop DNA probes templated by the nucleic acid targets to form a double stem-loop DNA, which subsequently initiates the isothermally exponential amplification reaction with high amplification efficiency. With the proposed method, high sensitivity to determine as low as 0.01 fM DNA or 0.1 fM RNA targets and high specificity to detect single-base changes can be achieved. The new methodology is robust to be performed by using a pair of universal primers under isothermal conditions, which should be employed to quantitatively detect any genetic biomarkers because all DNA/RNA targets can be directly used as the templates to ligate the stem-loop DNA probes with single-base resolution.


Subject(s)
DNA/analysis , MicroRNAs/analysis , Nucleic Acid Amplification Techniques/methods , Bacteriophage T4/enzymology , Biomarkers/analysis , DNA/chemistry , DNA/genetics , DNA Ligases/chemistry , DNA Methylation , DNA Probes/chemistry , DNA Probes/genetics , Humans , Inverted Repeat Sequences , Limit of Detection , MCF-7 Cells , MicroRNAs/chemistry , MicroRNAs/genetics , Nucleic Acid Hybridization , Polymorphism, Single Nucleotide , RNA Ligase (ATP)/chemistry , Viral Proteins/chemistry
11.
Talanta ; 206: 120217, 2020 Jan 01.
Article in English | MEDLINE | ID: mdl-31514897

ABSTRACT

MicroRNAs (miRNA) involve in regulating different physiological processes whose dysregulation is associated with a wide range of diseases including cancers, diabetes and cardiovascular problems. Herein, we report a direct, sensitive and highly selective detection assay for circulating microRNA (miRNA). This detection strategy employs magnetic nanoparticles as the reaction platform which can not only allow online pre-concentration and selective separation but also integrates ligation reaction with amplification to enhance the sensitivity of the detection assay. With the presence of the target miRNA, the locked nucleic acid (LNA)-modified molecular beacon (MB) opens up, exposing the binding sites at two ends. The 3'- and 5'-end of the MB responsible for the attachment onto the magnetic nanoparticles, and reporting probe for the attachment of the pair of amplification probes respectively. The ligase ligate RNA to DNA enhance the amplification efficiency. Upon labelled with intercalating fluorophores (YOYO-1) on the hybrids, the quantification of the target miRNA was determined by measuring the fluorescence intensity. A detection limit of 314 fM was achieved with trace amount of sample consumption (~20 µL). As a proof of concept, miRNA-149 was chosen as the target miRNA. This assay is capable of discriminating single-base and reliably quantifying circulating miRNA-149 in both healthy and cancer patient's serums. The result obtained was comparable with that of quantitative reverse transcription polymerase chain reaction (qRT-PCR), suggesting that this direct and sensitive assay can be served as a promising, non-invasive tool for early diagnosis of breast cancer and colorectal cancer.


Subject(s)
Circulating MicroRNA/blood , MicroRNAs/blood , Microscopy, Fluorescence/methods , RNA Ligase (ATP)/chemistry , Viral Proteins/chemistry , Benzoxazoles/chemistry , Circulating MicroRNA/genetics , DNA/chemistry , DNA/genetics , Fluorescent Dyes/chemistry , Humans , Intercalating Agents/chemistry , Limit of Detection , Magnetite Nanoparticles/chemistry , MicroRNAs/genetics , Nucleic Acid Hybridization , Oligonucleotides/chemistry , Oligonucleotides/genetics , Proof of Concept Study , Quinolinium Compounds/chemistry
12.
J Am Chem Soc ; 141(50): 19625-19633, 2019 12 18.
Article in English | MEDLINE | ID: mdl-31751505

ABSTRACT

The catalytic use of a small peptide scaffold for the biosynthesis of amino acid-derived natural products is a recently discovered new biosynthetic strategy. During this process, a peptide-amino acyl tRNA ligase (PEARL) adds amino acids to the C-terminus of a small peptide scaffold in an ATP- and tRNA-dependent process. The mechanism of this unusual transformation is currently not known. In this study, we present a detailed biochemical and mechanistic study of TglB (UniProtKB-F3HQJ1), a PEARL that catalyzes the addition of Cys to the C-terminus of the peptide TglA in the biosynthesis of 3-thiaglutamate in the plant pathogen Pseudomonas syringae. TglB recognizes several important residues close to the C-terminus of TglA to perform its activity and is tolerant with respect to the last amino acid of its substrate peptide. The enzyme recognizes the acceptor stem of tRNACys, as micro- and minihelices, truncated versions of full-length tRNACys that contain the acceptor stem, were also accepted. Mutagenesis of conserved residues in TglB identified several key residues for catalysis and did not support the possibility of TglB adopting various ping-pong mechanisms to catalyze the amino acid addition reaction. Using isotopic labeling studies, we demonstrate that ATP is used to directly phosphorylate the C-terminal carboxylate of TglA. Collectively, the data support a general mechanism for the amino acid addition reaction catalyzed by this class of enzyme.


Subject(s)
Peptides/metabolism , RNA Ligase (ATP)/metabolism , RNA, Transfer, Amino Acyl/metabolism , Biocatalysis , Models, Molecular , Peptides/chemistry , Protein Conformation , RNA Ligase (ATP)/chemistry
13.
Nucleic Acids Res ; 47(22): 11826-11838, 2019 12 16.
Article in English | MEDLINE | ID: mdl-31722405

ABSTRACT

Fungal tRNA ligase (Trl1) rectifies RNA breaks with 2',3'-cyclic-PO4 and 5'-OH termini. Trl1 consists of three catalytic modules: an N-terminal ligase (LIG) domain; a central polynucleotide kinase (KIN) domain; and a C-terminal cyclic phosphodiesterase (CPD) domain. Trl1 enzymes found in all human fungal pathogens are untapped targets for antifungal drug discovery. Here we report a 1.9 Å crystal structure of Trl1 KIN-CPD from the pathogenic fungus Candida albicans, which adopts an extended conformation in which separate KIN and CPD domains are connected by an unstructured linker. CPD belongs to the 2H phosphotransferase superfamily by dint of its conserved central concave ß sheet and interactions of its dual HxT motif histidines and threonines with phosphate in the active site. Additional active site motifs conserved among the fungal CPD clade of 2H enzymes are identified. We present structures of the Candida Trl1 KIN domain at 1.5 to 2.0 Å resolution-as apoenzyme and in complexes with GTP•Mg2+, IDP•PO4, and dGDP•PO4-that highlight conformational switches in the G-loop (which recognizes the guanine base) and lid-loop (poised over the nucleotide phosphates) that accompany nucleotide binding.


Subject(s)
Catalytic Domain , Guanosine Triphosphate/metabolism , RNA Ligase (ATP)/chemistry , RNA Ligase (ATP)/metabolism , Amino Acid Sequence , Base Sequence , Candida albicans , Catalytic Domain/genetics , Crystallography, X-Ray , Models, Molecular , Nucleotidases/chemistry , Polynucleotide 5'-Hydroxyl-Kinase/chemistry , Protein Binding , Protein Conformation , Protein Folding , RNA Ligase (ATP)/genetics , Structure-Activity Relationship
14.
Nucleic Acids Res ; 47(17): 8950-8960, 2019 09 26.
Article in English | MEDLINE | ID: mdl-31504757

ABSTRACT

Template-directed RNA ligation catalyzed by an RNA enzyme (ribozyme) is a plausible and important reaction that could have been involved in transferring genetic information during prebiotic evolution. Laboratory evolution experiments have yielded several classes of ligase ribozymes, but their minimal sequence requirements remain largely unexplored. Because selection experiments strongly favor highly active sequences, less active but smaller catalytic motifs may have been overlooked in these experiments. We used large-scale DNA synthesis and high-throughput ribozyme assay enabled by deep sequencing to systematically minimize a previously laboratory-evolved ligase ribozyme. After designing and evaluating >10 000 sequences, we identified catalytic cores as small as 18 contiguous bases that catalyze template-directed regiospecific RNA ligation. The fact that such a short sequence can catalyze this critical reaction suggests that similarly simple or even simpler motifs may populate the RNA sequence space which could have been accessible to the prebiotic ribozymes.


Subject(s)
Directed Molecular Evolution , RNA Ligase (ATP)/chemistry , RNA Ligase (ATP)/genetics , RNA, Catalytic/chemistry , RNA, Catalytic/genetics , Catalysis , Catalytic Domain , DNA/biosynthesis , High-Throughput Nucleotide Sequencing , Models, Molecular , Nucleotide Motifs , RNA/genetics , RNA Ligase (ATP)/metabolism , RNA, Catalytic/metabolism , Substrate Specificity
15.
Biotechniques ; 67(4): 178-183, 2019 10.
Article in English | MEDLINE | ID: mdl-31462065

ABSTRACT

Until recently, RNA-RNA interactions were mainly identified by crosslinking RNAs with interacting proteins, RNA proximity ligation and deep sequencing. Recently, AMT-based direct RNA crosslinking was established. Yet, several steps of these procedures are rather inefficient, reducing the output of identified interaction partners. To increase the local concentration of RNA ends, interacting RNAs are often fragmented. However, the resulting 2',3'-cyclic phosphate and 5'-OH ends are not accepted by T4 RNA ligase and have to be converted to 3'-OH and 5'-phosphate ends. Using an artificial mRNA/sRNA pair, we optimized the workflow downstream of the crosslinking reaction in vitro. The use of a tRNA ligase allows direct fusion of 2',3'-cyclic phosphate and 5'-OH RNA ends.


Subject(s)
Genetic Techniques , RNA, Messenger/genetics , Arabidopsis Proteins/chemistry , Arabidopsis Proteins/metabolism , Cross-Linking Reagents/chemistry , Phosphates/chemistry , RNA Ligase (ATP)/chemistry , RNA Ligase (ATP)/metabolism , RNA, Messenger/chemistry , Workflow
16.
Analyst ; 144(14): 4180-4187, 2019 Jul 08.
Article in English | MEDLINE | ID: mdl-31123738

ABSTRACT

MicroRNAs (miRNAs) play important roles in gene regulation and have been reported as biomarkers in cancer diagnosis. Herein, we develop an isothermal miRNA detection platform based on the highly efficient, multiple primer-mediated rolling circle amplification method coupled with a graphene oxide-based fluorescence (MPRCA-GO) assay, using lung cancer-associated miRNAs (miR-21 and miR-210) and a reference miRNA (miR-16) as model targets. The combination of the designed ssDNA probe and T4 RNA ligase (T4 Rnl2) used in the MPRCA-GO assay allowed for single-base mismatch discrimination. In addition, the superfluorescence quenching ability of GO allowed for rapid fluorescence detection. The developed platform had a limit of detection as low as 0.87 fM and could detect target miRNAs in cancer cell lines and human serums. Therefore, the MPRCA-GO sensor has the potential for single nucleotide polymorphism (SNP) analysis and applications in clinical diagnostics.


Subject(s)
Biomarkers, Tumor/blood , Graphite/chemistry , MicroRNAs/blood , Bacteriophage T4/enzymology , Biomarkers, Tumor/genetics , Cell Line, Tumor , DNA Probes/genetics , DNA, Single-Stranded/genetics , Fluorescence , Humans , Limit of Detection , MicroRNAs/genetics , Nucleic Acid Amplification Techniques/methods , Nucleic Acid Hybridization , RNA Ligase (ATP)/chemistry , Spectrometry, Fluorescence/methods , Viral Proteins/chemistry
17.
J Biol Chem ; 293(35): 13524-13533, 2018 08 31.
Article in English | MEDLINE | ID: mdl-29997253

ABSTRACT

Multiple DNA damage response (DDR) pathways have evolved to sense the presence of damage and recruit the proper repair factors. We recently reported a signaling pathway induced upon alkylation damage to recruit the AlkB homolog 3, α-ketoglutarate-dependent dioxygenase (ALKBH3)-activating signal cointegrator 1 complex subunit 3 (ASCC3) dealkylase-helicase repair complex. As in other DDR pathways, the recruitment of these repair factors is mediated through a ubiquitin-dependent mechanism. However, the machinery that coordinates the proper assembly of this repair complex and controls its recruitment is still poorly defined. Here, we demonstrate that the ASCC1 accessory subunit is important for the regulation of ASCC complex function. ASCC1 interacts with the ASCC complex through the ASCC3 helicase subunit. We find that ASCC1 is present at nuclear speckle foci prior to damage, but leaves the foci in response to alkylation. Strikingly, ASCC1 loss significantly increases ASCC3 foci formation during alkylation damage, yet most of these foci lack ASCC2. These results suggest that ASCC1 coordinates the proper recruitment of the ASCC complex during alkylation, a function that appears to depend on a putative RNA-binding motif near the ASCC1 C terminus. Consistent with its role in alkylation damage signaling and repair, ASCC1 knockout through a CRISPR/Cas9 approach results in alkylation damage sensitivity in a manner epistatic with ASCC3. Together, our results identify a critical regulator of the ALKBH3-ASCC alkylation damage signaling pathway and suggest a potential role for RNA-interacting domains in the alkylation damage response.


Subject(s)
DNA Helicases/metabolism , Nuclear Proteins/metabolism , Protein Interaction Maps , Transcription Factors/metabolism , Alkylation , Amino Acid Sequence , Cell Line , DNA Damage , DNA Demethylation , DNA Repair , Humans , Models, Molecular , Protein Domains , RNA/metabolism , RNA Ligase (ATP)/chemistry , RNA Ligase (ATP)/metabolism , Transcription Factors/chemistry
18.
Nucleic Acids Res ; 45(22): 12945-12953, 2017 Dec 15.
Article in English | MEDLINE | ID: mdl-29165709

ABSTRACT

Fungal tRNA ligase (Trl1) is an essential enzyme that repairs RNA breaks with 2',3'-cyclic-PO4 and 5'-OH ends inflicted during tRNA splicing and non-canonical mRNA splicing in the fungal unfolded protein response. Trl1 is composed of C-terminal cyclic phosphodiesterase and central polynucleotide kinase domains that heal the broken ends to generate the 3'-OH,2'-PO4 and 5'-PO4 termini required for sealing by an N-terminal ligase domain. Trl1 enzymes are found in all human fungal pathogens and are promising targets for antifungal drug discovery because their domain compositions and biochemical mechanisms are unique compared to the mammalian RtcB-type tRNA splicing enzyme. A distinctive feature of Trl1 is its preferential use of GTP as phosphate donor for the RNA kinase reaction. Here we report the 2.2 Å crystal structure of the kinase domain of Trl1 from the fungal pathogen Candida albicans with GDP and Mg2+ in the active site. The P-loop phosphotransferase fold of the kinase is embellished by a unique 'G-loop' element that accounts for guanine nucleotide specificity. Mutations of amino acids that contact the guanine nucleobase efface kinase activity in vitro and Trl1 function in vivo. Our findings fortify the case for the Trl1 kinase as an antifungal target.


Subject(s)
Catalytic Domain , Fungal Proteins/metabolism , Guanosine Triphosphate/metabolism , RNA Ligase (ATP)/metabolism , RNA/metabolism , Amino Acid Sequence , Candida albicans/enzymology , Candida albicans/genetics , Crystallography, X-Ray , Fungal Proteins/chemistry , Fungal Proteins/genetics , Guanosine Triphosphate/chemistry , Magnesium/chemistry , Magnesium/metabolism , Models, Molecular , Mutation , Protein Binding , RNA/genetics , RNA Ligase (ATP)/chemistry , RNA Ligase (ATP)/genetics , Saccharomyces cerevisiae Proteins/chemistry , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Sequence Homology, Amino Acid , Substrate Specificity
19.
Mol Biol (Mosk) ; 51(4): 724-733, 2017.
Article in Russian | MEDLINE | ID: mdl-28900093

ABSTRACT

Currently, isothermal methods of nucleic acid amplification have been well established; in particular, rolling circle amplification is of great interest. In this approach, circular ssDNA molecules have been used as a target that can be obtained by the intramolecular template-dependent ligation of an oligonucleotide C-probe. Here, a new method of synthesizing small circular DNA molecules via the cyclization of ssDNA based on T4 RNA ligase has been proposed. Circular ssDNA is further used as the template for the rolling circle amplification. The maximum yield of the cyclization products was observed in the presence of 5-10% polyethylene glycol 4000, and the optimum DNA length for the cyclization constituted 50 nucleotides. This highly sensitive method was shown to detect less than 10^(2) circular DNA molecules. The method reliability was proved based on artificially destroyed dsDNA, which suggests its implementation for analyzing any significantly fragmented dsDNA.


Subject(s)
DNA, Circular/chemical synthesis , DNA, Single-Stranded/chemical synthesis , Nucleic Acid Amplification Techniques , RNA Ligase (ATP)/chemistry , Viral Proteins/chemistry , DNA Fragmentation , Oligonucleotide Probes/chemistry , Polyethylene Glycols/chemistry
20.
Methods Mol Biol ; 1648: 1-9, 2017.
Article in English | MEDLINE | ID: mdl-28766285

ABSTRACT

The 5'-cap structure is an essential feature in eukaryotic mRNA required for mRNA stability and enhancement of translation. Ceratin transcripts are selectively silenced by decapping in the cytoplasm and later become translationally active again by acquiring the cap structure to regenerate translatable mRNAs. Identification of uncapped mRNA transcripts will reveal how gene expression is regulated by the mRNA recapping pathway. What follows is a sensitive method to detect and identify the uncapped mRNA from the cells. The technique consists of three parts: selective ligation of anchor RNA to the 5'-end of monophosphate RNA by double-strand RNA ligase, conversion of ligated RNA product into cDNA by reverse transcription, and amplification of a specific cDNA by polymerase chain reaction.


Subject(s)
Ligase Chain Reaction/methods , RNA Ligase (ATP)/chemistry , RNA, Messenger , RNA, Protozoan , Reverse Transcriptase Polymerase Chain Reaction/methods , Trypanosoma brucei brucei , DNA, Complementary/biosynthesis , DNA, Complementary/chemistry , DNA, Complementary/genetics , RNA, Messenger/chemistry , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Protozoan/chemistry , RNA, Protozoan/genetics , RNA, Protozoan/metabolism , Trypanosoma brucei brucei/genetics , Trypanosoma brucei brucei/metabolism
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