ABSTRACT
1. IgM-like immunoglobulin fractions have been isolated by combinations of precipitation procedures and gel-filtration from the serum of three vertebrate species, representative of the classes of Pisces (Cyprinus carpio L.), Amphibia (Rana ridibunda Pall.) and Reptilia (Testudo graeca Pall.). 2. The homogeneity of the isolated fractions has been confirmed electrophoretically and immunoelectrophoretically. 3. Electrophoretic mobility of IgM, similar for the three species studied has been observed in polyacrylamide gel; in agar gel immunoelectrophoresis carp and frog IgM mobilities are almost identical, in contrast to the markedly slower cathodic migration of tortoise IgM.
Subject(s)
Carps/immunology , Cyprinidae/immunology , Immunoglobulin M/analysis , Rana ridibunda/immunology , Ranidae/immunology , Turtles/immunology , Animals , Carps/blood , Chemical Precipitation , Chromatography, Gel , Electrophoresis, Polyacrylamide Gel , Immunodiffusion , Immunoelectrophoresis , Immunoglobulin M/isolation & purification , Lipid Metabolism , Rana ridibunda/blood , Species Specificity , Turtles/bloodABSTRACT
1. By successive application of gel-filtration and ion-exchange chromatography IgG fraction have been isolated from frog (Rana ridibunda Pall.) and tortoise (Testudo graeca Pall.) sera. 2. The homogeneity of the fractions has been confirmed and a similar electrophoretic mobility has been revealed by polyacrylamide gel electrophoresis and immunoelectrophoresis. 3. In rabbits polyclonal antibodies giving specific reaction with the corresponding IgG immunoglobulin have been raised.
