ABSTRACT
OBJECTIVE: We examined whether the vitrification method using P10 and PEPeS is suitable for the cryopreservation of two-cell stage embryos collected from multiple rat strains with the objective of strain preservation of inbred rat strains. RESULTS: The average numbers of two-cell stage embryos collected per female for F344/Jcl, ACI/N, BUF/N, and WKY/N strains were 7.0 to 12.0, and survival rates of the embryos after vitrification were 94.2 to 96.3 % The in vitro development rates of vitrified embryos transferred were 47.1 to 60.8 %. CONCLUSION: At least two offspring produced from the embryos collected from one female are required for strain preservation of inbred strain. Taken together, the results of the experiment indicated expected numbers of surviving fetuses for embryos collected from one female were 3.2 to 6.7, and all were usable for strain preservation. The results suggest that this vitrification method is suitable for strain preservation of multiple inbred rat strains.
Subject(s)
Cryopreservation/methods , Rats, Inbred Strains/embryology , Vitrification , Animals , Cryopreservation/veterinary , Embryo Transfer , Embryonic Development , Female , Male , Rats, Inbred ACI/embryology , Rats, Inbred BUF/embryology , Rats, Inbred F344/embryology , Rats, Inbred WKY/embryologyABSTRACT
The ACI rat with its known incidence of ipsilateral agenesis of the kidney and ductus deferens provides an excellent mammalian model for the study of the embryologic origin or the caput epididymidis. Because a portion of the caput epididymidis remains in these affected animals, it seems to be derived from the mesonephric tubules rather than the mesonephric duct in the rat. Histochemical assays also support the common origin of both the ductuli efferentes and caput epididymidis from the mesonephric tubules.
Subject(s)
Epididymis/embryology , Mesonephros/cytology , Rats, Inbred ACI/embryology , Rats, Inbred Strains/embryology , Alkaline Phosphatase/analysis , Animals , Disease Models, Animal , Epididymis/cytology , Epididymis/enzymology , Esterases/analysis , Female , Glycerolphosphate Dehydrogenase/analysis , Kidney/abnormalities , Kidney/embryology , Male , Mesonephros/enzymology , NADPH Dehydrogenase/analysis , Pregnancy , Rats , Seminal Vesicles/abnormalities , Succinate Dehydrogenase/analysis , Testis/cytology , Testis/embryology , Vas Deferens/abnormalities , Vas Deferens/embryologyABSTRACT
The ACI rat provides a congenital mammalian model for the spontaneously occurring defects of renal agenesis and hydronephrosis. These anomalies appear to be part of a spectrum of abnormalities caused by a mesonephric duct defect. These same anomalies also exist in man probably more commonly than previously appreciated.