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1.
J Infect Dis ; 217(2): 198-207, 2018 01 04.
Article in English | MEDLINE | ID: mdl-29140433

ABSTRACT

Background: Clostridium difficile is a leading cause of nosocomial infection. The role of cytokine interleukin-27 (IL-27) in the immunopathology of C. difficile infection (CDI) remains unknown. Methods: The production of IL-27 was determined in human and murine CDI. Furthermore, wild-type (WT) and IL-27 receptor-deficient (WSX-1-/-) mice were treated with an antibiotic mixture and infected with C. difficile to investigate the effects of IL-27 on host response to CDI. Results: IL-27 production was elevated during CDI in humans and mice. Infected WSX-1-/- mice experienced increased weight loss, enhanced colonic histology damage, less C. difficile clearance, and decreased survival compared to WT controls during CDI. IL-27 administration reduced CDI-associated mortality and tissue pathology with improved C. difficile clearance in WT mice after C. difficile challenge. Mechanistically, IL-27-mediated host defense against CDI was associated with downregulation of IL-17A and IL-23, but upregulation of IL-10 and interferon-gamma during CDI. Conclusions: Our data suggest a previously unrecognized role for IL-27 in the pathogenesis of CDI, potentially providing new insight for IL-27-mediated protection against C. difficile-induced pathology.


Subject(s)
Clostridioides difficile/immunology , Clostridium Infections/immunology , Colitis/immunology , Interleukins/metabolism , Receptors, Cytokine/metabolism , Adult , Aged , Animals , Body Weight , Clostridium Infections/pathology , Colitis/pathology , Female , Histocytochemistry , Humans , Male , Mice, Inbred C57BL , Mice, Knockout , Middle Aged , Receptors, Cytokine/deficiency , Receptors, Interleukin , Signal Transduction , Survival Analysis
2.
Sci Rep ; 7: 43469, 2017 02 24.
Article in English | MEDLINE | ID: mdl-28233860

ABSTRACT

Pulmonary alveolar proteinosis (PAP) is a rare pulmonary disease in which the abnormalities in alveolar surfactant accumulation are caused by impairments of GM-CSF pathway attributing to defects in a variety of genes. However, hereditary PAP is extremely uncommon and a detailed understanding in the genetic inheritance of PAP in a family may provide timely diagnosis, treatment and proper intervention including genetic consultation. Here, we described a comprehensive analysis of genome and gene expression for a family containing one affected child with a diagnosis of PAP and two other healthy siblings. Family-based whole-genome analysis revealed a homozygous deletion that disrupts CSF2RA, CRLF2, and IL3RA gene in the pseudoautosomal region of the X chromosome in the affected child and one of asymptomatic siblings. Further functional pathway analysis of differentially expressed genes in IL-1ß-treated peripheral blood mononuclear cells highlighted the insufficiency of immune response in the child with PAP, especially the protection against bacterial infection. Collectively, our results reveal a novel allele as the genetic determinant of a family with PAP and provide insights into variable expressivity and incomplete penetrance of this rare disease, which will be helpful for proper genetic consultation and prompt treatment to avoid mortality and morbidity.


Subject(s)
Chromosomes, Human, X/chemistry , Genetic Diseases, Inborn/genetics , Interleukin-3 Receptor alpha Subunit/deficiency , Pulmonary Alveolar Proteinosis/genetics , Receptors, Cytokine/deficiency , Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/deficiency , Asymptomatic Diseases , Child , Child, Preschool , Female , Gene Deletion , Genetic Diseases, Inborn/diagnosis , Genetic Diseases, Inborn/immunology , Genetic Diseases, Inborn/pathology , Homozygote , Humans , Interleukin-1beta/pharmacology , Interleukin-3 Receptor alpha Subunit/genetics , Interleukin-3 Receptor alpha Subunit/immunology , Leukocytes, Mononuclear/drug effects , Leukocytes, Mononuclear/immunology , Leukocytes, Mononuclear/pathology , Macrophages, Alveolar/immunology , Macrophages, Alveolar/pathology , Male , Pedigree , Penetrance , Primary Cell Culture , Pulmonary Alveolar Proteinosis/diagnosis , Pulmonary Alveolar Proteinosis/immunology , Pulmonary Alveolar Proteinosis/pathology , Receptors, Cytokine/genetics , Receptors, Cytokine/immunology , Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/genetics , Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/immunology , Severity of Illness Index , Siblings , Whole Genome Sequencing
3.
J Immunol ; 198(1): 119-127, 2017 01 01.
Article in English | MEDLINE | ID: mdl-27881708

ABSTRACT

EBI3 functions as the subunit of immune-regulatory cytokines, such as IL-27 and IL-35, by pairing with p28 and p35, respectively. We treated wild-type and EBI3-deficient mice with intratracheal administration of LPS and obtained bronchoalveolar lavage fluid (BALF) 24 h later. Although neutrophils were the predominant cells in BALF from both groups of mice, eosinophils were highly enriched and there was increased production of eosinophil-attracting chemokines CCL11 and CCL24 in BALF of EBI3-deficient mice. The bronchial epithelial cells and alveolar macrophages were the major producers of CCL11 and CCL24. Because no such increases in eosinophils were seen in BALF of p28/IL-27-deficient mice or WSX-1/IL-27Rα subunit-deficient mice upon intratracheal stimulation with LPS, we considered that the lack of IL-35 was responsible for the enhanced airway eosinophilia in EBI3-deficient mice. In vitro, IL-35 potently suppressed production of CCL11 and CCL24 by human lung epithelial cell lines treated with TNF-α and IL-1ß. IL-35 also suppressed phosphorylation of STAT1 and STAT3 and induced suppressor of cytokine signaling 3. In vivo, rIL-35 dramatically reduced LPS-induced airway eosinophilia in EBI3-deficient mice, with concomitant reduction of CCL11 and CCL24, whereas neutralization of IL-35 significantly increased airway eosinophils in LPS-treated wild-type mice. Collectively, our results suggest that IL-35 negatively regulates airway eosinophilia, at least in part by reducing the production of CCL11 and CCL24.


Subject(s)
Interleukins/immunology , Minor Histocompatibility Antigens/immunology , Pulmonary Eosinophilia/immunology , Receptors, Cytokine/immunology , Animals , Cell Line , Chemokine CCL11/biosynthesis , Chemokine CCL24/biosynthesis , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Humans , Immunoblotting , Immunohistochemistry , Interleukins/metabolism , Lipopolysaccharides/immunology , Lipopolysaccharides/toxicity , Mice , Mice, Inbred C57BL , Mice, Knockout , Polymerase Chain Reaction , Pulmonary Eosinophilia/pathology , Receptors, Cytokine/deficiency
4.
Methods Mol Biol ; 1422: 127-36, 2016.
Article in English | MEDLINE | ID: mdl-27246028

ABSTRACT

Eosinophilic esophagitis (EoE) is an immune-mediated disorder characterized by esophageal inflammation and related structural changes causing symptoms such as feeding difficulties and food impaction. The pathophysiological mechanisms underlying EoE remain poorly understood. Preclinical studies using mouse models have been critical in comprehending human disease mechanisms and associated pathways. In this chapter, we describe an experimental method using a noninvasive label-free optical imaging technique, optical coherence tomography, to characterize the pathophysiological changes in the esophagus of mice with EoE-like disease ex vivo.


Subject(s)
Eosinophilic Esophagitis/pathology , Esophagus/pathology , Tomography, Optical Coherence/methods , Animals , Disease Models, Animal , Eosinophilic Esophagitis/diagnostic imaging , Eosinophilic Esophagitis/genetics , Eosinophilic Esophagitis/physiopathology , Gene Knockdown Techniques , Humans , Immunoglobulins/deficiency , Mice , Receptors, Cytokine/deficiency
5.
J Gen Virol ; 97(6): 1481-1487, 2016 06.
Article in English | MEDLINE | ID: mdl-26935332

ABSTRACT

Microglial activation is a hallmark of the neuroimmunological response to Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis and prion disease. The CX3C chemokine axis consists of fractalkine (CX3CL1) and its receptor (CX3CR1); these are expressed by neurons and microglia respectively, and are known to modulate microglial activation. In prion-infected mice, both Cx3cr1 and Cx3cl1 are altered, suggesting a role in disease. To investigate the influence of CX3C axis signalling on prion disease, we infected Cx3cr1 knockout (Cx3cr1-KO) and control mice with scrapie strains 22L and RML. Deletion of Cx3cr1 had no effect on development of clinical signs or disease incubation period. In addition, comparison of brain tissue from Cx3cr1-KO and control mice revealed no significant differences in cytokine levels, spongiosis, deposition of disease-associated prion protein or microglial activation. Thus, microglial activation during prion infection did not require CX3C axis signalling.


Subject(s)
Microglia/pathology , Prion Diseases/genetics , Prion Diseases/pathology , Receptors, Chemokine/genetics , Animals , Brain/pathology , CX3C Chemokine Receptor 1 , Mice , Mice, Knockout , Receptors, Chemokine/metabolism , Receptors, Cytokine/deficiency , Receptors, Cytokine/metabolism , Receptors, HIV/deficiency , Receptors, HIV/metabolism , Signal Transduction
6.
Am J Respir Cell Mol Biol ; 55(2): 264-74, 2016 08.
Article in English | MEDLINE | ID: mdl-26934097

ABSTRACT

The mechanisms that contribute to homeostasis of the immune system in sepsis are largely unknown. One study suggests a potential detrimental role for thymic stromal lymphopoietin (TSLP) in sepsis; however, the immune-regulatory effects of TSLP on myeloid cells within the intestinal microenvironment suggest the contrary. Our objective was to clarify TSLP's role in sepsis. Cecal ligation and puncture was performed in mice with total or myeloid-specific deficiency in the TSLP receptor (TSLPR). Survival was monitored closely, peritoneal fluids and plasma were analyzed for markers of inflammation, and myeloid cell numbers and their ability to produce inflammatory mediators was determined. The interaction of TSLP with TSLPR in myeloid cells contributed to mouse survival after septic peritonitis. Mice with TSLPR deficiency in myeloid cells displayed excessive local and systemic inflammation levels (e.g., increased inflammatory cell and cytokine levels) relative to control mice. Moreover, hepatic injury was exacerbated in mice with TSLPR deficiency in their myeloid cells. However, the enhanced inflammatory response did not affect the ability of these mice to clear bacteria. Resident neutrophils and macrophages from septic mice with TSLPR deficiency exhibited an increased ability to produce proinflammatory cytokines. Collectively, our findings suggest that the effects of TSLP on myeloid cells are crucial in reducing the multiple organ failure that is associated with systemic inflammation, which highlights the significance of this cytokine in modulating the host response to infection and in reducing the risks of sepsis development.


Subject(s)
Cytokines/metabolism , Inflammation/metabolism , Inflammation/pathology , Sepsis/metabolism , Sepsis/pathology , Animals , Down-Regulation , Humans , Immunoglobulins/deficiency , Immunoglobulins/metabolism , Inflammation/complications , Inflammation Mediators/metabolism , Interleukin-6/metabolism , Male , Mice, Inbred C57BL , Myeloid Cells/metabolism , Protein Binding , Receptors, Cytokine/deficiency , Receptors, Cytokine/metabolism , Sepsis/complications , Signal Transduction , Survival Analysis , Thymic Stromal Lymphopoietin
7.
J Allergy Clin Immunol ; 138(1): 283-286, 2016 07.
Article in English | MEDLINE | ID: mdl-26830114
8.
Hepatology ; 63(3): 1000-12, 2016 Mar.
Article in English | MEDLINE | ID: mdl-26637970

ABSTRACT

UNLABELLED: The cellular and molecular etiology of unresolved chronic liver inflammation remains obscure. Whereas mutant p53 has gain-of-function properties in tumors, the role of this protein in liver inflammation is unknown. Herein, mutant p53(R172H) is mechanistically linked to spontaneous and sustained liver inflammation and steatosis when combined with the absence of interleukin-27 (IL27) signaling (IL27RA), resembling the phenotype observed in nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) patients. Indeed, these mice develop, with age, hepatocyte necrosis, immune cell infiltration, fibrosis, and micro- and macrosteatosis; however, these phenotypes are absent in mutant p53(R172H) or IL27RA(-/-) mice. Mechanistically, endothelin A receptor (ETAR)-positive macrophages are highly accumulated in the inflamed liver, and chemical inhibition of ETAR signaling reverses the observed phenotype and negatively regulates mutant p53 levels in macrophages. CONCLUSION: The combination of mutant p53 and IL27RA(-/-) causes spontaneous liver inflammation, steatosis, and fibrosis in vivo, whereas either gene alone in vivo has no effects on the liver.


Subject(s)
Genes, p53 , Macrophages/metabolism , Non-alcoholic Fatty Liver Disease/genetics , Receptor, Endothelin A/metabolism , Receptors, Cytokine/deficiency , Animals , Mice, Inbred C57BL , Mice, Transgenic , Non-alcoholic Fatty Liver Disease/immunology , Non-alcoholic Fatty Liver Disease/metabolism , Receptors, Interleukin
9.
Tuberculosis (Edinb) ; 95(4): 497-504, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25937126

ABSTRACT

Tuberculosis (TB) remains a major global health problem and host genetic factors play a critical role in susceptibility and resistance to TB. The aim of this study was to identify novel candidate genes associated with TB susceptibility. We performed a population-based case-control study to genotype 13 tag SNPs spanning Epstein-Barr virus-induced gene 3 (EBI3), colony stimulating factor 2 (CSF2), IL-4, interferon beta 1 (IFNB1), chemokine (C-X-C motif) ligand 14 (CXCL14) and myeloid differentiation primary response gene 88 (Myd88) genes in 435 pulmonary TB patients and 375 health donors from China. We observed that EBI3 gene rs4740 polymorphism was associated with susceptibility to pulmonary tuberculosis (PTB) and the allele G was associated with a protective effect against PTB. Furthermore, EBI3 deficiency led to reduced bacterial burden and histopathological impairment in the lung of mice infected with Mycobacterium bovis BCG. Meanwhile, higher abundance of EBI3 was observed in the granuloma of PTB patients and in the lung tissue of BCG-infected mice. Of note, the expression of EBI3 in macrophages was remarkably induced by mycobacteria infection at both mRNA and protein level. In conclusion, EBI3 gene rs4740 polymorphism is closely associated with susceptibility to PTB and the elevation and enrichment of EBI3 in the lung which at least partially derived from macrophages may contribute to the exacerbation of mycobacterial infection.


Subject(s)
Interleukins/genetics , Lung/metabolism , Mycobacterium tuberculosis/pathogenicity , Polymorphism, Single Nucleotide , Receptors, Cytokine/genetics , Tuberculosis, Pulmonary/genetics , Animals , Bacterial Load , Case-Control Studies , Cell Line , China , Disease Models, Animal , Genetic Predisposition to Disease , Host-Pathogen Interactions , Humans , Interleukins/metabolism , Lung/microbiology , Macrophages/microbiology , Mice, Inbred C57BL , Mice, Knockout , Minor Histocompatibility Antigens , Mycobacterium bovis/pathogenicity , Phenotype , Protective Factors , Receptors, Cytokine/deficiency , Risk Factors , Tuberculosis, Pulmonary/diagnosis , Tuberculosis, Pulmonary/metabolism , Tuberculosis, Pulmonary/microbiology , Tuberculosis, Pulmonary/prevention & control
10.
J Invest Dermatol ; 135(8): 2109-2118, 2015 Aug.
Article in English | MEDLINE | ID: mdl-25789702

ABSTRACT

Several inflammatory diseases including scleroderma and atopic dermatitis display dermal thickening, epidermal hypertrophy, or excessive accumulation of collagen. Factors that might promote these features are of interest for clinical therapy. We previously reported that LIGHT, a TNF superfamily molecule, mediated collagen deposition in the lungs in response to allergen. We therefore tested whether LIGHT might similarly promote collagen accumulation and features of skin fibrosis. Strikingly, injection of recombinant soluble LIGHT into naive mice, either subcutaneously or systemically, promoted collagen deposition in the skin and dermal and epidermal thickening. This replicated the activity of bleomycin, an antibiotic that has been previously used in models of scleroderma in mice. Moreover skin fibrosis induced by bleomycin was dependent on endogenous LIGHT activity. The action of LIGHT in vivo was mediated via both of its receptors, HVEM and LTßR, and was dependent on the innate cytokine TSLP and TGF-ß. Furthermore, we found that HVEM and LTßR were expressed on human epidermal keratinocytes and that LIGHT could directly promote TSLP expression in these cells. We reveal an unappreciated activity of LIGHT on keratinocytes and suggest that LIGHT may be an important mediator of skin inflammation and fibrosis in diseases such as scleroderma or atopic dermatitis.


Subject(s)
Keratinocytes/physiology , Skin/pathology , Skin/physiopathology , Tumor Necrosis Factor Ligand Superfamily Member 14/physiology , Animals , Bleomycin/pharmacology , Cells, Cultured , Collagen/metabolism , Disease Models, Animal , Female , Fibrosis/metabolism , Fibrosis/pathology , Fibrosis/physiopathology , Humans , Immunoglobulins/deficiency , Immunoglobulins/genetics , Immunoglobulins/metabolism , Infant, Newborn , Keratinocytes/drug effects , Mice , Mice, Inbred C57BL , Mice, Knockout , Receptors, Cytokine/deficiency , Receptors, Cytokine/genetics , Receptors, Cytokine/metabolism , Receptors, Tumor Necrosis Factor, Member 14/deficiency , Receptors, Tumor Necrosis Factor, Member 14/genetics , Receptors, Tumor Necrosis Factor, Member 14/metabolism , Skin/metabolism , Tumor Necrosis Factor Ligand Superfamily Member 14/deficiency , Tumor Necrosis Factor Ligand Superfamily Member 14/genetics
11.
Eur J Immunol ; 45(6): 1680-95, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25763996

ABSTRACT

Human metapneumovirus (hMPV) is a leading cause of acute respiratory tract infections in children and the elderly. The mechanism by which this virus triggers an inflammatory response still remains unknown. Here, we evaluated whether the thymic stromal lymphopoietin (TSLP) pathway contributes to lung inflammation upon hMPV infection. We found that hMPV infection promotes TSLP expression both in human airway epithelial cells and in the mouse lung. hMPV infection induced lung infiltration of OX40L(+) CD11b(+) DCs. Mice lacking the TSLP receptor deficient mice (tslpr(-/-) ) showed reduced lung inflammation and hMPV replication. These mice displayed a decreased number of neutrophils as well a reduction in levels of thymus and activation-regulated chemokine/CCL17, IL-5, IL-13, and TNF-α in the airways upon hMPV infection. Furthermore, a higher frequency of CD4(+) and CD8(+) T cells was found in tslpr(-/-) mice compared to WT mice, which could contribute to controlling viral spread. Depletion of neutrophils in WT and tslpr(-/-) mice decreased inflammation and hMPV replication. Remarkably, blockage of TSLP or OX40L with specific Abs reduced lung inflammation and viral replication following hMPV challenge in mice. Altogether, these results suggest that activation of the TSLP pathway is pivotal in the development of pulmonary pathology and pulmonary hMPV replication.


Subject(s)
Cytokines/metabolism , Metapneumovirus/physiology , Paramyxoviridae Infections/metabolism , Paramyxoviridae Infections/virology , Pneumonia, Viral/metabolism , Pneumonia, Viral/virology , Signal Transduction , Animals , Antibodies, Monoclonal/administration & dosage , Antibodies, Monoclonal/pharmacology , Cell Line , Cytokines/genetics , Dendritic Cells/immunology , Dendritic Cells/metabolism , Disease Models, Animal , Epithelial Cells/metabolism , Epithelial Cells/virology , Gene Expression , Humans , Interleukin-33 , Interleukin-8/genetics , Interleukin-8/metabolism , Interleukins/genetics , Interleukins/metabolism , Macrophages, Alveolar/immunology , Macrophages, Alveolar/metabolism , Metapneumovirus/drug effects , Mice , Neutrophils/immunology , Neutrophils/metabolism , OX40 Ligand/antagonists & inhibitors , OX40 Ligand/genetics , OX40 Ligand/metabolism , Paramyxoviridae Infections/drug therapy , Paramyxoviridae Infections/genetics , Paramyxoviridae Infections/pathology , Pneumonia, Viral/drug therapy , Pneumonia, Viral/genetics , Pneumonia, Viral/pathology , Receptors, Cytokine/antagonists & inhibitors , Receptors, Cytokine/deficiency , Signal Transduction/drug effects , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , T-Lymphocyte Subsets/pathology , Virus Replication , Thymic Stromal Lymphopoietin
12.
Respir Res ; 16: 10, 2015 Feb 05.
Article in English | MEDLINE | ID: mdl-25651926

ABSTRACT

BACKGROUND: Influenza is a common respiratory virus and Staphylococcus aureus frequently causes secondary pneumonia during influenza infection, leading to increased morbidity and mortality. Influenza has been found to attenuate subsequent Type 17 immunity, enhancing susceptibility to secondary bacterial infections. IL-27 is known to inhibit Type 17 immunity, suggesting a potential critical role for IL-27 in viral and bacterial co-infection. METHODS: A murine model of influenza and Staphylococcus aureus infection was used to mimic human viral, bacterial co-infection. C57BL/6 wild-type, IL-27 receptor α knock-out, and IL-10 knock-out mice were infected with Influenza H1N1 (A/PR/8/34) or vehicle for 6 days followed by challenge with Staphylococcus aureus or vehicle for 24 hours. Lung inflammation, bacterial burden, gene expression, and cytokine production were determined. RESULTS: IL-27 receptor α knock-out mice challenged with influenza A had increased morbidity compared to controls, but no change in viral burden. IL-27 receptor α knock-out mice infected with influenza displayed significantly decreased IL-10 production compared to wild-type. IL-27 receptor α knock-out mice co-infected with influenza and S. aureus had improved bacterial clearance compared to wild-type controls. Importantly, there were significantly increased Type 17 responses and decreased IL-10 production in IL-27 receptor α knock-out mice. Dual infected IL-10-/- mice had significantly less bacterial burden compared to dual infected WT mice. CONCLUSIONS: These data reveal that IL-27 regulates enhanced susceptibility to S. aureus pneumonia following influenza infection, potentially through the induction of IL-10 and suppression of IL-17.


Subject(s)
Coinfection , Influenza A Virus, H1N1 Subtype/immunology , Interleukins/immunology , Lung/immunology , Orthomyxoviridae Infections/immunology , Pneumonia, Staphylococcal/immunology , Staphylococcus aureus/immunology , Animals , Bacterial Load , Disease Models, Animal , Host-Pathogen Interactions , Immunity, Cellular , Influenza A Virus, H1N1 Subtype/pathogenicity , Interleukin-10/deficiency , Interleukin-10/genetics , Interleukin-17/immunology , Lung/microbiology , Lung/virology , Male , Mice, Inbred C57BL , Mice, Knockout , Orthomyxoviridae Infections/virology , Pneumonia, Staphylococcal/genetics , Pneumonia, Staphylococcal/microbiology , Receptors, Cytokine/deficiency , Receptors, Cytokine/genetics , Receptors, Interleukin , Staphylococcus aureus/pathogenicity , Th17 Cells/immunology , Th17 Cells/microbiology , Th17 Cells/virology , Time Factors , Viral Load
13.
Cell Physiol Biochem ; 35(1): 160-74, 2015.
Article in English | MEDLINE | ID: mdl-25591759

ABSTRACT

AIMS: Thymic stromal lymphopoietin (TSLP) plays an important role in inflammatory diseases and is over-expressed in human atherosclerotic artery specimens. The present study investigated the role of TSLP in platelet activation and thrombosis models in vitro and in vivo, as well as the underlying mechanism and signaling pathway. METHODS AND RESULTS: Western blotting and flow cytometry demonstrated that the TSLP receptor was expressed on murine platelets. According to flow cytometry, platelet stimulation with TSLP induced platelet degranulation and integrin αIIbß3 activation. A TSLPR deficiency caused defective platelet aggregation, defective platelet secretion and markedly blunted thrombus growth in perfusion chambers at both low and high shear rates. TSLPR KO mice exhibited defective carotid artery thrombus formation after exposure to FeCl3. TSLP increased Akt phosphorylation, an effect that was abrogated by the PI3K inhibitors wortmannin and LY294002. The PI3K inhibitors further diminished TSLP-induced platelet activation. TSLP-mediated platelet degranulation, integrin αIIbß3 activation and Akt phosphorylation were blunted in platelets that lacked the TSLP receptor. CONCLUSION: This study demonstrated that the functional TSLPR was surface-expressed on murine platelets. The inflammatory cytokine TSLP triggered platelet activation and thrombus formation via TSLP-dependent PI3K/Akt signaling, which suggests an important role for TSLP in linking vascular inflammation and thrombo-occlusive diseases.


Subject(s)
Blood Platelets/metabolism , Cytokines/pharmacology , Immunoglobulins/metabolism , Platelet Activation/drug effects , Platelet Aggregation/drug effects , Receptors, Cytokine/metabolism , Signal Transduction/drug effects , Androstadienes/pharmacology , Animals , Blood Platelets/drug effects , Carotid Artery Thrombosis/chemically induced , Carotid Artery Thrombosis/metabolism , Carotid Artery Thrombosis/pathology , Chlorides/toxicity , Chromones/pharmacology , Disease Models, Animal , Ferric Compounds/toxicity , Humans , Immunoglobulins/deficiency , Immunoglobulins/genetics , Mice , Mice, Inbred C57BL , Mice, Knockout , Morpholines/pharmacology , P-Selectin/metabolism , Phosphatidylinositol 3-Kinases/metabolism , Phosphoinositide-3 Kinase Inhibitors , Phosphorylation/drug effects , Platelet Glycoprotein GPIIb-IIIa Complex/metabolism , Proto-Oncogene Proteins c-akt/metabolism , Receptors, Cytokine/deficiency , Receptors, Cytokine/genetics , Wortmannin , Thymic Stromal Lymphopoietin
14.
Proc Natl Acad Sci U S A ; 111(46): 16472-7, 2014 Nov 18.
Article in English | MEDLINE | ID: mdl-25267651

ABSTRACT

An elusive goal of cellular immune vaccines is the generation of large numbers of antigen-specific T cells in response to subunit immunization. A broad spectrum of cytokines and cell-surface costimulatory molecules are known to shape the programming, magnitude, and repertoire of T cells responding to vaccination. We show here that the majority of innate immune receptor agonist-based vaccine adjuvants unexpectedly depend on IL-27 for eliciting CD4(+) and CD8(+) T-cell responses. This is in sharp contrast to infectious challenge, which generates T-cell responses that are IL-27-independent. Mixed bone marrow chimera experiments demonstrate that IL-27 dependency is T cell-intrinsic, requiring T-cell expression of IL-27Rα. Further, we show that IL-27 dependency not only dictates the magnitude of vaccine-elicited T-cell responses but also is critical for the programming and persistence of high-affinity T cells to subunit immunization. Collectively, our data highlight the unexpected central importance of IL-27 in the generation of robust, high-affinity cellular immune responses to subunit immunization.


Subject(s)
Adaptive Immunity/immunology , Bacterial Vaccines/immunology , Interleukins/physiology , T-Lymphocyte Subsets/immunology , Vaccination , Adjuvants, Immunologic , Adoptive Transfer , Animals , CD40 Antigens/immunology , Female , Immunologic Memory , Listeriosis/immunology , Listeriosis/prevention & control , Lymphocyte Activation , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Ovalbumin/immunology , Poly I-C/immunology , Radiation Chimera , Receptors, Cytokine/deficiency , Receptors, Cytokine/physiology , Receptors, Interleukin , STAT Transcription Factors/deficiency , STAT Transcription Factors/physiology , Smallpox Vaccine/immunology , T-Cell Antigen Receptor Specificity , Toll-Like Receptors/immunology , Transcription, Genetic/immunology , Vaccines, Subunit/immunology , Vaccinia/immunology , Vaccinia/prevention & control
15.
Biochem Biophys Res Commun ; 451(2): 215-21, 2014 Aug 22.
Article in English | MEDLINE | ID: mdl-25088998

ABSTRACT

IL-27 is a heterodimeric cytokine that regulates both innate and adaptive immunity. The immunosuppressive effect of IL-27 largely depends on induction of IL-10-producing Tr1 cells. To date, however, effects of IL-27 on regulation of immune responses via mediators other than cytokines remain poorly understood. To address this issue, we examined immunoregulatory effects of conditional medium of bone marrow-derived macrophages (BMDMs) from WSX-1 (IL-27Rα)-deficient mice and found enhanced IFN-γ and IL-17A secretion by CD4(+) T cells as compared with that of control BMDMs. We then found that PGE2 production and COX-2 expression by BMDMs from WSX-1-deficient mice was increased compared to control macrophages in response to LPS. The enhanced production of IFN-γ and IL-17A was abolished by EP2 and EP4 antagonists, demonstrating PGE2 was responsible for enhanced cytokine production. Murine WSX-1-expressing Raw264.7 cells (mWSX-1-Raw264.7) showed phosphorylation of both STAT1 and STAT3 in response to IL-27 and produced less amounts of PGE2 and COX-2 compared to parental RAW264.7 cells. STAT1 knockdown in parental RAW264.7 cells and STAT1-deficiency in BMDMs showed higher COX-2 expression than their respective control cells. Collectively, our result indicated that IL-27/WSX-1 regulated PGE2 secretion via STAT1-COX-2 pathway in macrophages and affected helper T cell response in a PGE2-mediated fashion.


Subject(s)
Dinoprostone/biosynthesis , Interleukins/metabolism , Macrophages/immunology , Macrophages/metabolism , T-Lymphocytes, Helper-Inducer/immunology , Animals , Cell Line , Culture Media, Conditioned , Cyclooxygenase 2/metabolism , Female , Gene Knockdown Techniques , Interferon-gamma/biosynthesis , Interleukin-17/biosynthesis , Mice , Mice, Inbred C57BL , Mice, Knockout , Receptors, Cytokine/deficiency , Receptors, Cytokine/genetics , Receptors, Cytokine/metabolism , Receptors, Interleukin , STAT1 Transcription Factor/deficiency , STAT1 Transcription Factor/genetics , STAT1 Transcription Factor/metabolism , Signal Transduction , T-Lymphocytes, Helper-Inducer/metabolism
16.
Exp Eye Res ; 125: 107-13, 2014 Aug.
Article in English | MEDLINE | ID: mdl-24929202

ABSTRACT

Murine experimental autoimmune uveitis (EAU) is a model for human autoimmune uveitis, whose pathogenesis is caused by both Th1 and Th17 cell responses. Epstein-Barr virus-induced gene 3 (EBI3) is a component of the heterodimeric cytokines: interleukin (IL)-27 and IL-35. Although IL-27 was shown to initiate Th1 cell development, it is also recognized as a negative regulator of fully activated CD4+ T cells, including Th17 cells. Recently, IL-35 also has also been reported to play immunosuppressive roles in autoimmunity. To investigate the roles of EBI3 in EAU, EBI3(-/-) mice were immunized with human interphotoreceptor retinoid binding protein peptide 1-20 (IRBP) to induce EAU. We observed that the clinical score in EBI3(-/-) mice was diminished compared with that in EBI3(+/+) mice up to day 22 after immunization, whereas the score in EBI3(-/-) mice reached the same levels as that of EBI3(+/+) mice after day 28. Histological analysis revealed a significant reduction of cellular infiltration into the retina in EBI3(-/-) mice on day 16. Although Th1 cell responses and IRBP-specific IL-10 production were reduced in EBI3(-/-) mice, the development of Th17 cell responses was unaffected on day 9. On day 21, Th1 cell responses and IRBP-specific IL-10 production was restored to the same levels as that in EBI3(+/+) mice, and Th17 cell responses significantly increased in EBI3(-/-) mice. Furthermore, Foxp3 expression in CD4+ T cells was comparable between EBI3(+/+) and EBI3(-/-) mice on days 9 and 21. Therefore, these results indicate that EBI3 may be important in EAU initiation by Th1 cell responses and may suppress EAU by inhibition of both Th1 and Th17 cell responses in the late/maintenance phase.


Subject(s)
Autoimmune Diseases , Receptors, Cytokine/physiology , Uveitis , Animals , Autoimmune Diseases/immunology , Autoimmune Diseases/metabolism , Disease Models, Animal , Female , Forkhead Transcription Factors/metabolism , Gene Expression Regulation , Interleukins/metabolism , Mice , Mice, Inbred C57BL , Minor Histocompatibility Antigens , Receptors, Cytokine/deficiency , Th1 Cells/immunology , Th17 Cells/immunology , Uveitis/immunology , Uveitis/metabolism
17.
EMBO Mol Med ; 6(1): 120-40, 2014 01.
Article in English | MEDLINE | ID: mdl-24408967

ABSTRACT

Postinfluenza pneumococcal pneumonia is a common cause of death in humans. However, the role of IL-27 in the pathogenesis of secondary pneumococcal pneumonia after influenza is unknown. We now report that influenza infection induced pulmonary IL-27 production in a type I IFN-α/ß receptor (IFNAR) signalling-dependent manner, which sensitized mice to secondary pneumococcal infection downstream of IFNAR pathway. Mice deficient in IL-27 receptor were resistant to secondary pneumococcal infection and generated more IL-17A-producing γδ T cells but not αß T cells, thereby leading to enhanced neutrophil response during the early phase of host defence. IL-27 treatment could suppress the development of IL-17A-producing γδ T cells activated by Streptococcus pneumoniae and dendritic cells. This suppressive activity of IL-27 on γδ T cells was dependent on transcription factor STAT1. Finally, neutralization of IL-27 or administration of IL-17A restored the role of γδ T cells in combating secondary pneumococcal infection. Our study defines what we believe to be a novel role of IL-27 in impairing host innate immunity against pneumococcal infection.


Subject(s)
Interleukin-27/metabolism , Orthomyxoviridae Infections/complications , Pneumonia, Pneumococcal/etiology , Pneumonia, Pneumococcal/metabolism , Signal Transduction , Animals , Antibodies, Neutralizing/immunology , Cells, Cultured , Dendritic Cells/immunology , Humans , Influenza A Virus, H1N1 Subtype/physiology , Interleukin-17/genetics , Interleukin-17/immunology , Interleukin-17/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Orthomyxoviridae Infections/virology , Pneumonia, Pneumococcal/mortality , Receptors, Cytokine/deficiency , Receptors, Cytokine/genetics , Receptors, Cytokine/metabolism , Receptors, Interleukin , STAT1 Transcription Factor/metabolism , Streptococcus pneumoniae/physiology , Survival Rate , T-Lymphocytes/immunology , T-Lymphocytes/metabolism , T-Lymphocytes/microbiology
18.
Scand J Immunol ; 78(5): 408-18, 2013 Nov.
Article in English | MEDLINE | ID: mdl-24033709

ABSTRACT

The 4T1 mammary carcinoma cell line produces TSLP. We had hypothesized that TSLP promotes the development of a permissive environment for the growth and metastasis of primary tumour and that this is associated with a Th2-polarized antitumour immune response. We found that, in Tslpr(-/-) mice, the mean tumour diameters were smaller from days 27 to 40, and relatively fewer tumour cells were present in the lung, compared with wild-type mice. Polarization of the Th2 cytokine profile was also diminished in Tslpr(-/-) mice. These findings confirmed those reported previously by others. Here, we further show that primary tumours are established less often in Tslpr(-/-) mice and that, unexpectedly, the relative number of tumour cells in the brain is greater in Tslpr(-/-) mice compared with wild-type mice. Findings from our cytotoxicity assays show that 4T1-directed lysis is undetectable in both WT and Tslpr(-/-) mice, ruling out the possibility that altered cytotoxic responses in Tslpr(-/-) mice are responsible for the differences we observed. In a human tissue microarray, positive staining for TSLP was seen in tumour cells from breast cancer tissue, but it was also seen in normal glandular epithelial cells from normal breast tissue, which has not been shown before. Thus, our findings provide new insight into the effects of TSLP in metastatic breast cancer.


Subject(s)
Brain Neoplasms/metabolism , Breast Neoplasms/metabolism , Immunoglobulins/genetics , Lung Neoplasms/metabolism , Receptors, Cytokine/genetics , Th2 Cells/immunology , Animals , Cell Line, Tumor , Cell Proliferation , Cytokines/metabolism , Female , Humans , Immunoglobulins/deficiency , Mice , Mice, Inbred BALB C , Mice, Knockout , Receptors, Cytokine/deficiency , Th2 Cells/metabolism , Tissue Array Analysis
19.
J Immunol ; 191(4): 1657-65, 2013 Aug 15.
Article in English | MEDLINE | ID: mdl-23836062

ABSTRACT

IL-27 is an APC-derived IL-6/IL-12 family composite cytokine with multiple functions such as regulation of Th1, Th17, and regulatory T cell differentiation, B cell proliferation, and Ig class switching. The IL-27 complex is formed by the association of the cytokine p28 with the soluble cytokine receptor EBV-induced gene 3 (EBI3). The IL-27 cytokine and soluble receptor subunits p28 and EBI3 can be secreted independently. The p28 subunit has been shown to have IL-27-independent biological activities. We previously demonstrated that p28 can form an alternative composite cytokine with the EBI3 homolog cytokine-like factor 1 (CLF; CRLF1). p28/CLF modulates NK cell activity and CD4 T cell cytokine production in vitro. In this study we used IL-6-dependent plasmacytoma cell line B9 and CD4 T cells from IL-27Rα-deficient mice to demonstrate that p28/CLF activates IL-27-unresponsive cells, indicating that p28/CLF and IL-27 signal through different receptors. The observation that p28/CLF, unlike IL-27, sustains B9 plasmacytoma cell proliferation prompted us to investigate the effects of p28/CLF on mouse B cells. We observed that p28/CLF induces IgM, IgG2c, and IgG1 production and plasma cell differentiation. p28/CLF therefore has the potential to contribute to B and plasma cell function, differentiation, and proliferation in normal and pathological conditions such as Castelman's disease and multiple myeloma.


Subject(s)
B-Lymphocytes/cytology , Interleukins/immunology , Lymphopoiesis/physiology , Plasma Cells/cytology , Animals , B-Lymphocytes/immunology , Cell Division , Cell Line , Female , Immunoglobulins/biosynthesis , Interleukins/genetics , Janus Kinases/physiology , Lipopolysaccharides/immunology , Mice , Mice, Inbred C57BL , Mice, Knockout , Phosphorylation , Plasma Cells/immunology , Protein Processing, Post-Translational , Receptors, Cytokine/deficiency , Receptors, Cytokine/genetics , Receptors, Interleukin , Recombinant Fusion Proteins/immunology , STAT Transcription Factors/physiology , Signal Transduction , Th2 Cells/immunology , Transfection
20.
J Interferon Cytokine Res ; 33(11): 700-8, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23772952

ABSTRACT

To ensure survival, our immune system must overcome the action of pathogen-encoded immune antagonists, such as influenza A nonstructural protein-1 (NS1). NS1 subverts the host interferon (IFN) response at multiple levels and blocks the induction of IFN-ß, a critical antiviral cytokine. This immune antagonism can be overcome in some cases. It has been shown that IFN-ß is upregulated by 48 h in the lungs of wild-type C57BL/6 mice infected with influenza A. In contrast, it is shown here that IFNB1 continues to be repressed in IFNAR1(-/-) IL28Rα(-/-) mice, which are deficient in Type-I and III IFN signaling, implying induction of IFNB1 depends on effective IFN signaling. Despite the complete lack of IFN signaling in this system, some IFN stimulated genes (ISGs) were induced following infection with a Flu strain lacking NS1. While the expression of these viral stress-inducible genes (VSIGs) was initially repressed following infection with wild-type Flu, many of these genes became upregulated by 48 h postinfection. These results demonstrate the existence of IFN-independent mechanisms that can overcome NS1-mediated immune antagonism of VSIGs.


Subject(s)
Immune Evasion , Influenza A virus/immunology , Interferons/immunology , Viral Nonstructural Proteins/metabolism , Animals , Interferons/genetics , Mice , Mice, Inbred C57BL , Mice, Knockout , Receptor, Interferon alpha-beta/deficiency , Receptor, Interferon alpha-beta/genetics , Receptor, Interferon alpha-beta/immunology , Receptors, Cytokine/deficiency , Receptors, Cytokine/genetics , Receptors, Cytokine/immunology , Signal Transduction/genetics , Signal Transduction/immunology
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