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1.
PLoS One ; 12(3): e0174694, 2017.
Article in English | MEDLINE | ID: mdl-28350844

ABSTRACT

The sigma1 receptor acts as a chaperone at the endoplasmic reticulum, associates with multiple proteins in various cellular systems, and involves in a number of diseases, such as addiction, pain, cancer and psychiatric disorders. The sigma1 receptor is encoded by the single copy SIGMAR1 gene. The current study identifies five alternatively spliced variants of the mouse sigma1 receptor gene using a polymerase chain reaction cloning approach. All the splice variants are generated by exon skipping or alternative 3' or 5' splicing, producing the truncated sigma1 receptor. Similar alternative splicing has been observed in the human SIGMAR1 gene based on the molecular cloning or genome sequence prediction, suggesting conservation of alternative splicing of SIGMAR1 gene. Using quantitative polymerase chain reactions, we demonstrate differential expression of several splice variants in mouse tissues and brain regions. When expressed in HEK293 cells, all the splice variants fail to bind sigma ligands, implicating that each truncated region in these splice variants is important for ligand binding. However, co-immunoprecipitation (Co-IP) study in HEK293 cells co-transfected with tagged constructs reveals that all the splice variants maintain their ability to physically associate with a mu opioid receptor (mMOR-1), providing useful information to correlate the motifs/sequences necessary for their physical association. Furthermore, a competition Co-IP study showed that all the variants can disrupt in a dose-dependent manner the dimerization of the original sigma1 receptor with mMOR-1, suggesting a potential dominant negative function and providing significant insights into their function.


Subject(s)
Alternative Splicing , Exons/genetics , Gene Expression Profiling/methods , Receptors, sigma/genetics , Amino Acid Sequence , Animals , Cloning, Molecular , HEK293 Cells , Humans , Male , Mice, Inbred C57BL , Models, Genetic , Polymerase Chain Reaction , Protein Binding , Protein Isoforms/genetics , Protein Isoforms/isolation & purification , Protein Isoforms/metabolism , Receptors, sigma/isolation & purification , Receptors, sigma/metabolism , Sequence Homology, Amino Acid , Sigma-1 Receptor
2.
Protein Expr Purif ; 89(2): 203-9, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23562661

ABSTRACT

Sigma 1 receptor (S1R) is a eukaryotic membrane protein that functions as an inter-organelle signaling modulator and chaperone. Here we report an improved expression of S1R in Escherichia coli as a fusion to maltose binding protein (MBP) and a high-yield purification. Variants with linking amino acid sequences consisting of 0-5 alanine residues between MBP and S1R were created and tested in several E. coli expression strains in order to determine the best combination of construct and host for production of active MBP-S1R. Among the linker variations, the protein containing a 4-Ala linker exhibited superior expression characteristics (MBP-4A-S1R); this construct was most productively paired with E. coli B834-pRARE2 and a chemically defined growth and expression medium. A 3-step purification was developed, including extraction from the E. coli membrane fraction using a mixture of Triton X-100 and n-dodecyl-beta-D-maltopyranoside identified by screening constrainted by retention of binding function, and purification by amylose affinity and gel filtration chromatographies. This procedure yields ∼3.5mg of purified fusion protein per L of bacterial culture medium. Purified MBP-4A-S1R showed a 175-fold purification from the starting cellular lysate with respect to specific ligand binding activity, and is stable during concentration and freeze-thaw cycling.


Subject(s)
Cloning, Molecular , Escherichia coli/genetics , Maltose-Binding Proteins/genetics , Maltose-Binding Proteins/isolation & purification , Receptors, sigma/genetics , Receptors, sigma/isolation & purification , Amino Acid Sequence , Animals , Base Sequence , Genetic Vectors/genetics , Guinea Pigs , Maltose-Binding Proteins/chemistry , Molecular Sequence Data , Plasmids/genetics , Receptors, sigma/chemistry , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/isolation & purification , Sigma-1 Receptor
3.
J Biol Chem ; 287(44): 37021-9, 2012 Oct 26.
Article in English | MEDLINE | ID: mdl-22952230

ABSTRACT

The sigma-1 receptor (Sig1R) is up-regulated in many human tumors and plays a role in the control of cancer cell proliferation and invasiveness. At the molecular level, the Sig1R modulates the activity of various ion channels, apparently through a direct interaction. We have previously shown using atomic force microscopy imaging that the Sig1R binds to the trimeric acid-sensing ion channel 1A with 3-fold symmetry. Here, we investigated the interaction between the Sig1R and the Nav1.5 voltage-gated Na(+) channel, which has also been implicated in promoting the invasiveness of cancer cells. We show that the Sig1R and Nav1.5 can be co-isolated from co-transfected cells, consistent with an intimate association between the two proteins. Atomic force microscopy imaging of the co-isolated proteins revealed complexes in which Nav1.5 was decorated by Sig1Rs. Frequency distributions of angles between pairs of bound Sig1Rs had two peaks, at ∼90° and ∼180°, and the 90° peak was about twice the size of the 180° peak. These results demonstrate that the Sig1R binds to Nav1.5 with 4-fold symmetry. Hence, each set of six transmembrane regions in Nav1.5 likely constitutes a Sig1R binding site, suggesting that the Sig1R interacts with the transmembrane regions of its partners. Interestingly, two known Sig1R ligands, haloperidol and (+)-pentazocine, disrupted the Nav1.5/Sig1R interaction both in vitro and in living cells. Finally, we show that endogenously expressed Sig1R and Nav1.5 also functionally interact.


Subject(s)
NAV1.5 Voltage-Gated Sodium Channel/metabolism , Receptors, sigma/metabolism , Cell Line , Chromatography, Affinity , Gene Knockdown Techniques , Haloperidol/chemistry , Humans , Ligands , Membrane Potentials , Microscopy, Atomic Force , NAV1.5 Voltage-Gated Sodium Channel/chemistry , NAV1.5 Voltage-Gated Sodium Channel/isolation & purification , Pentazocine/chemistry , Protein Binding , Protein Multimerization , Protein Structure, Quaternary , RNA Interference , Receptors, sigma/chemistry , Receptors, sigma/genetics , Receptors, sigma/isolation & purification , Single-Cell Analysis , Sigma-1 Receptor
4.
Protein Expr Purif ; 51(2): 283-92, 2007 Feb.
Article in English | MEDLINE | ID: mdl-16962337

ABSTRACT

Sigma receptors once considered as a class of opioid receptors are now regarded as unique orphan receptors, distinguished by the ability to bind various pharmacological agents such as the progesterone (steroid), haloperidol (anti-psychotic), and drugs of abuse such as cocaine and methamphetamine. The sigma-1 receptor is a 223 amino acid protein, proposed to have two transmembrane segments. We have developed a scheme for the purification of the guinea pig sigma-1 receptor following overexpression in Escherichia coli as a maltose binding protein (MBP) fusion and extraction with Triton X-100. Affinity chromatography using an amylose column and Ni2+ affinity column was used to purify the sigma-1 receptor. The sigma-1 receptor purified by this method is a 26 kDa polypeptide as assessed by SDS-PAGE, binds sigma ligands with high affinity and can be specifically photoaffinity labeled with the sigma-1 receptor photoprobe, [125I]-iodoazidococaine. Ligand binding using [3H]-(+)-pentazocine indicated that approximately half of the purified protein in Triton X-100 bound to radioligand. The MBP-sigma-1 receptor and the sigma-1 receptor in 0.5% triton were maximally stable for approximately two weeks at -20 degrees C in buffer containing 30% glycerol.


Subject(s)
Receptors, sigma/isolation & purification , Receptors, sigma/metabolism , Animals , Carrier Proteins/genetics , Drug Stability , Escherichia coli/metabolism , Factor Xa/metabolism , Guinea Pigs , Ligands , Maltose-Binding Proteins , Pentazocine/metabolism , Receptors, sigma/immunology , Recombinant Fusion Proteins/isolation & purification , Recombinant Fusion Proteins/metabolism , Sigma-1 Receptor
5.
Eur J Pharmacol ; 493(1-3): 19-28, 2004 Jun 16.
Article in English | MEDLINE | ID: mdl-15189760

ABSTRACT

We have previously shown that sigma-2 receptors are relatively difficult to solubilize (Eur. J. Pharmacol. 304 (1996) 201), suggesting possible localization in detergent-resistant lipid raft domains. Rat liver membranes were treated on ice with 1% Triton X-100 or 20 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), and the extract subjected to centrifugation on a discontinuous gradient of 5%, 38%, and 40% sucrose. Gradient fractions were analyzed for sigma-1 receptors using [3H]+-pentazocine and for sigma-2 receptors using [3H]1,3-di-o-tolylguanidine ([3H]DTG), in the presence of dextrallorphan. Flotillin-2 was assessed by immunoblotting as a marker for lipid rafts. Sigma-2 receptors were found to discretely co-localize with flotillin-2 in lipid raft fractions. However, sigma-1 receptors were found throughout the gradient. Rafts prepared in CHAPS had sigma-2 receptors with normal pharmacological characteristics, whereas those in Triton X-100-prepared rafts had about seven-fold lower affinity for [3H]DTG and other ligands. Thus, sigma-2 receptors are resident in membrane lipid rafts, whereas sigma-1 receptors appear in both raft and non-raft membrane domains. Lipid rafts may play an important role in the mechanism of sigma-2 receptor-induced apoptosis.


Subject(s)
Cell Membrane/pathology , Liver/cytology , Membrane Microdomains/physiology , Receptors, sigma/physiology , Animals , Cell Membrane/chemistry , Cholic Acids/chemistry , Cholic Acids/pharmacology , Endothelial Cells/chemistry , Guanidines/pharmacology , Humans , Immunoblotting/methods , Levallorphan/pharmacology , Liver Extracts/chemistry , Male , Membrane Microdomains/chemistry , Membrane Proteins/chemistry , Membrane Proteins/isolation & purification , National Institutes of Health (U.S.) , Nerve Tissue Proteins/chemistry , Nerve Tissue Proteins/isolation & purification , Octoxynol/chemistry , Octoxynol/pharmacology , Pentazocine/pharmacology , Radioligand Assay , Rats , Rats, Sprague-Dawley , Receptors, sigma/chemistry , Receptors, sigma/isolation & purification , Tritium , United States , Sigma-1 Receptor
6.
Proc Natl Acad Sci U S A ; 93(15): 8072-7, 1996 Jul 23.
Article in English | MEDLINE | ID: mdl-8755605

ABSTRACT

Sigma-ligands comprise several chemically unrelated drugs such as haloperidol, pentazocine, and ditolylguanidine, which bind to a family of low molecular mass proteins in the endoplasmic reticulum. These so-called sigma-receptors are believed to mediate various pharmacological effects of sigma-ligands by as yet unknown mechanisms. Based on their opposite enantioselectivity for benzomorphans and different molecular masses, two subtypes are differentiated. We purified the sigma1-binding site as a single 30-kDa protein from guinea pig liver employing the benzomorphan(+)[3H]pentazocine and the arylazide (-)[3H]azidopamil as specific probes. The purified (+)[3H]pentazocine-binding protein retained its high affinity for haloperidol, pentazocine, and ditolylguanidine. Partial amino acid sequence obtained after trypsinolysis revealed no homology to known proteins. Radiation inactivation of the pentazocine-labeled sigma1-binding site yielded a molecular mass of 24 +/- 2 kDa. The corresponding cDNA was cloned using degenerate oligonucleotides and cDNA library screening. Its open reading frame encoded a 25.3-kDa protein with at least one putative transmembrane segment. The protein expressed in yeast cells transformed with the cDNA showed the pharmacological characteristics of the brain and liver sigma1-binding site. The deduced amino acid sequence was structurally unrelated to known mammalian proteins but it shared homology with fungal proteins involved in sterol synthesis. Northern blots showed high densities of the sigma1-binding site mRNA in sterol-producing tissues. This is also in agreement with the known ability of sigma1-binding sites to interact with steroids, such as progesterone.


Subject(s)
Brain/metabolism , Microsomes, Liver/metabolism , Microsomes/metabolism , Receptors, sigma/metabolism , Amino Acid Sequence , Animals , Base Sequence , Binding Sites , Binding, Competitive , Chromatography, Affinity , Chromatography, Ion Exchange , Cloning, Molecular , DNA Primers , DNA, Complementary , DNA-Binding Proteins/chemistry , Guinea Pigs , Intracellular Membranes/metabolism , Kinetics , Ligands , Mammals , Molecular Sequence Data , Pentazocine/metabolism , Polymerase Chain Reaction , Receptors, sigma/chemistry , Receptors, sigma/isolation & purification , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Recombinant Proteins/isolation & purification , Saccharomyces cerevisiae , Sequence Homology, Amino Acid , Trans-Activators/chemistry , Transcriptional Regulator ERG
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