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1.
ANZ J Surg ; 88(9): E639-E643, 2018 09.
Article in English | MEDLINE | ID: mdl-29569820

ABSTRACT

BACKGROUND: Rectal cancer care has become increasingly complex and requires accurate information. The pathology report is a vital tool for accessing information to gauge a patient's prognosis and to guide treatment decisions. The aim of this study was to assess the quality of histopathological reporting and surgery for rectal cancer in New Zealand using defined quality indicators. METHODS: This is a retrospective audit of pathological reports of all resected rectal cancer pathology reports submitted to the New Zealand Cancer Registry (NZCR) in 2015. The quality of reporting was assessed using specified criteria: synoptic report, adequate lymph node retrieval, reporting of circumferential resection margin (CRM) and mesorectal excision quality. Surgical outcomes were sphincter preservation rate, CRM clearance and complete mesorectal excision. RESULTS: A total of 803 patients with rectal cancer were reported to the NZCR in 2015, 505 underwent proctectomy. A total of 89.5% of reports were structured, 81.8% reported mesorectal excision quality and 86.7% reported CRM status. Adequate lymph node retrieval was obtained in 65.1%, complete mesorectal excision in 84.6% and positive CRM in 6.2% of cases. Quality varied between laboratories and district health boards. High-volume laboratories had higher quality reporting. Surgeon volume and training was related to adequate lymph node retrieval but not CRM clearance nor mesorectal excision quality. CONCLUSION: High-quality pathological reporting is associated with the use of synoptic reporting templates. Surgical outcomes for rectal cancer in New Zealand, especially the low rate of CRM involvement, compare favourably with international audits.


Subject(s)
Clinical Audit/methods , Pathology, Clinical/methods , Quality Indicators, Health Care/standards , Rectal Neoplasms/surgery , Aged , Female , Humans , Lymph Nodes/pathology , Male , Margins of Excision , Middle Aged , Neoplasm Staging , New Zealand/epidemiology , Pathology, Clinical/standards , Proctectomy/methods , Rectal Neoplasms/mortality , Rectal Neoplasms/ultrastructure , Retrospective Studies , Treatment Outcome
2.
Zhonghua Wei Chang Wai Ke Za Zhi ; 18(3): 252-6, 2015 Mar.
Article in Chinese | MEDLINE | ID: mdl-25809329

ABSTRACT

OBJECTIVE: To evaluate the accuracy of endorectal ultrasound (ERUS) in predicting the circumferential resection margin (CRM) and maximum tumor thickness (MTT) of in T3 rectal cancer. METHODS: Clinicl data of 53 patients with pT3 rectal cancer admitted to the Department of General Surgery in the Peking Union Medical College Hospital from June 2011 to January 2014 were retrospectively analyzed. CRM and MTT measured by ERUS were compared with corresponding pathologic measurements to assess the accuracy of ERUS diagnosis. RESULTS: ERUS correctly predicted CRM status in 52 patients (98.1%, 52/53), whose sensitivity was 100%, specificity was 97.8%, positive predictive value was 85.7%, and negative predictive value was 100%. ERUS correctly predicted MTT status in 51 patients (96.2%, 51/53), whose sensitivity was 100%, specificity was 95.5%, positive predictive value was 66.6%, and negative predictive value was 100%. In the Bland and Altman plot, the agreement between ERUS and pathology was good. CONCLUSION: Endorectal ultrasonography can accurately diagnose CRM and MTT, which can satisfy the clinical need for preoperative staging of rectal cancer.


Subject(s)
Rectal Neoplasms/ultrastructure , Colectomy , Humans , Neoplasm Staging , Peritoneum , Retrospective Studies
3.
Pathol Res Pract ; 209(4): 244-8, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23484779

ABSTRACT

Perivascular epithelioid cell tumor (PEComa) is a rare collection of tumors arising in a wide array of anatomic locations. It is characterized by the presence of a peculiar population of myomelanocytic marker-positive perivascular epithelioid cells, and is commonly detected in the uterus. The colorectal area is an uncommon site for primary PEComa. In this study, we describe a 17-year-old patient presenting with a rectal polyp. Histologically, the tumor consisted of sheets of round to polygonal epithelioid cells with clear and granular cytoplasm, and a prominent capillary network. Some of the tumor cells were positive for Fontana-Masson staining. Immunohistochemically, the tumor cells were positive for HMB-45, and were negative for cytokeratin, vimentin, S-100 protein, actin, desmin, EMA, CD34, and c-kit. After finding melanosomes or premelanosomes at the ultrastructural level, the diagnosis of PEComa was made. Although PEComa arising within the intestinal tract is unusual and clinically unexpected, PEComa should be considered in the differential diagnosis of rectal polypoid lesions.


Subject(s)
Intestinal Polyps/pathology , Perivascular Epithelioid Cell Neoplasms/pathology , Rectal Neoplasms/pathology , Adolescent , Adult , Biomarkers, Tumor/analysis , Biopsy , Child , Colonoscopy , Female , Humans , Immunohistochemistry , Intestinal Polyps/chemistry , Intestinal Polyps/surgery , Intestinal Polyps/ultrastructure , Male , Melanosomes/pathology , Middle Aged , Perivascular Epithelioid Cell Neoplasms/chemistry , Perivascular Epithelioid Cell Neoplasms/surgery , Perivascular Epithelioid Cell Neoplasms/ultrastructure , Predictive Value of Tests , Rectal Neoplasms/chemistry , Rectal Neoplasms/surgery , Rectal Neoplasms/ultrastructure
4.
Int. j. morphol ; 30(1): 25-29, mar. 2012. ilus
Article in English | LILACS | ID: lil-638754

ABSTRACT

The micropapillary carcinoma is regarded as an aggressive variant of adenocarcinoma in any location. Histologically is characterized by papillary cell clusters surrounded by clear spaces. The reported proportion of micropapillary carcinoma component to the entire tumor ranged from 5 to 80 percent and no pure cases has been reported. There are near of 130 cases reported to date in colorectum. We experienced a patient with a pure micropapillary carcinoma showing coexpression of CK7, CK20, and absence of CDX2, which had an aggressive neoplasm with extense perineural, vascular and lymphatic invasion also extensive nodal metastasis. The presence of a micropapillary carcinoma in the colorectum seemed to be closely related with nodal metastasis, similar to the case for micropapillary carcinomas in other organs. Therefore, if a micropapillary component is identified in a tumor, particularly in a biopsy specimen, even if the pre-operative diagnosis is a pedunculated early colorectal cancer, should be carefully consider the extent of surgical resection due to the high potential for nodal metastasis.


El carcinoma micropapilar es considerado como una variante agresiva del cáncer en cualquier localización. Histológicamente se caracteriza por grupos de células papilares rodeada de espacios libres. Se informó que la proporción del componente carcinoma micropapilar en la totalidad de un tumor varió entre 5 por ciento a 80 por ciento y no se han reportado casos puros. Existen cerca de 130 casos reportados hasta la fecha en colon y recto. Se describe el caso de un paciente con un carcinoma micropapilar puro que muestra coexpresión de CK7, CK20, y la ausencia de CDX2, que tenía un tumor agresivo con extensa invasión perineural, vascular y linfática además de metástasis nodular extensa. La presencia de un carcinoma micropapilar en la región colorrectal parece estar estrechamente relacionada con metástasis nodular, similar al caso del carcinomas micropapilar en otros órganos. Por lo tanto, si un componente micropapilar se identifica en un tumor, sobre todo en una muestra de biopsia, incluso si el diagnóstico pre-operatorio es un cáncer colorrectal temprano pediculado, se debe considerar cuidadosamente la extensión de la resección quirúrgica debido a la alta probabilidad de metástasis nodular.


Subject(s)
Aged , Carcinoma, Papillary , Rectal Neoplasms/enzymology , Rectal Neoplasms/ultrastructure , Adenocarcinoma/enzymology , Adenocarcinoma/ultrastructure , Gene Expression Regulation, Neoplastic
5.
Histochem Cell Biol ; 129(2): 223-32, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18034259

ABSTRACT

Recent studies suggest that the morphological and physiological properties of tight junctions (TJs) are determined by the combination and mixing ratios of claudin isoforms. In this study, we tried to characterize mouse cell lines by expression of claudin isoforms to use for studying epithelial TJs by overexpression or suppression of claudin(s) in the cells and found that claudin-2 was expressed in a few mouse rectum carcinoma cells, CMT93 cells. We have isolated CMT93-I and -II cells from CMT93 cells by immunohistochemical screening for the presence or absence of claudin-2 expression. Immunofluorescence and RT-PCR analyses showed that expression of claudin-4, -6, -7 and -12 was detected in both cell lines, but claudin-2 was only expressed in CMT93-II cells. There were no differences in paracellular permeability between CMT93-I and -II cells examined by 4 kDa FITC-dextran and fluorescein sodium, or in the number of TJ strands examined by freeze-fracture electron microscopy. However, the transepithelial electrical resistance (TER) of CMT93-I cells was approximately 6.5 times higher than that of CMT93-II cells, suggesting that expression of claudin-2 may be related to decreased TER. Comparative examinations of CMT93-I and -II cells provide a clue how the combination and mixing ratios of claudin isoforms regulate the paracellular permeability.


Subject(s)
Carcinoma/metabolism , Carcinoma/ultrastructure , Membrane Proteins/biosynthesis , Rectal Neoplasms/metabolism , Rectal Neoplasms/ultrastructure , Tight Junctions/metabolism , Tight Junctions/ultrastructure , Animals , Cell Line , Cell Membrane/physiology , Claudins , Cryoelectron Microscopy , Electric Impedance , Fibroblasts/metabolism , Fibroblasts/ultrastructure , Freeze Fracturing , Immunoblotting , Immunohistochemistry , Membrane Proteins/genetics , Mice , Protein Isoforms/biosynthesis , Protein Isoforms/genetics , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction
6.
Ultrastruct Pathol ; 31(4): 315-20, 2007.
Article in English | MEDLINE | ID: mdl-17786832

ABSTRACT

Rectal/anorectal malignant melanomas are highly aggressive tumors with a poor prognosis and low 5-year survival rate. They are also very rare. Of the well-known histological variants of malignant melanoma, the small cell subtype is also very uncommon; consequently, small cell anorectal malignant melanoma is an exceedingly rare occurrence. In this article, the authors provide a detailed clinicopathological description of small cell malignant melanoma of the rectum, documenting clinical, histological, immunohistochemical, and ultrastructural features, to add to the sparse references on this tumor in the literature. The patient was a 53-year-old woman with a mass 2 cm from the anus, which was surgically removed. In histological sections, the tumor was a small cell malignant melanoma, with a tumor cell diameter of 7.6+/-1.0 microm, and a range of 5.5-10.7 microm (N = 100). Tumor cells were positive for S-100 protein and HMB-45 and contained sparse but unambiguous type II melanosomes. This article is one of the few detailed clinicopathological documentations of a small cell malignant melanoma of the rectum (anorectum) and the first to have the diagnosis confirmed ultrastructurally by the identification of melanosomes. The present case adds to the 3 mainly or entirely small cell anorectal malignant melanomas described in the literature. There are also at least 12 other cases with less well-defined numbers of small tumor cells or with small cells admixed with other cell morphologies. Documentation of these unusual morphological variants is important for identifying any distinctive outcome they might exhibit compared with conventional malignant melanoma.


Subject(s)
Melanoma/ultrastructure , Rectal Neoplasms/ultrastructure , Antigens, Neoplasm , Female , Humans , Immunohistochemistry , Melanoma/metabolism , Melanoma-Specific Antigens , Microscopy, Electron, Transmission , Middle Aged , Neoplasm Proteins/biosynthesis , Rectal Neoplasms/metabolism
7.
World J Gastroenterol ; 12(11): 1757-60, 2006 Mar 21.
Article in English | MEDLINE | ID: mdl-16586547

ABSTRACT

AIM: To investigate the morphological characterization of tumor infiltrating dendritic cells (TIDCs) and tumor infiltrating lymphocytes (TILs) in human rectal cancer. METHODS: Light and electron microscopy as well as immunohistochemistry were used to observe the distributive and morphological changes of TIDCs and TILs. RESULTS: TIDCs were mainly located in tumor-surrounding tissue. The number of TIDCs in the earlier stage was higher than that in the later stage (P<0.01). TILs were mainly seen in adjacent tissue of cancers and tumor-surrounding tissue. There were more TILs in the earlier stage than that in the later stage (P<0.01). Under electron microscope, TIDCs were irregular in shape and exhibited many dendritic protrusions. It is not obvious that cancer cells perforated the basement membrane and TILs were arranged along the basement membrane in the earlier stage. In the later stage, it is explicit that cancer cells perforated the basement membrane and surrounded by TILs. There were contacts among TIDCs, TILs and tumor cell. One TIDCs contacted one or several TILs which clustered around TIDCs. Glycogen granules were seen between TIDCs and TILs. CONCLUSION: The number of TIDCs and TILs is related with tumor progression There exist close relationships among TIDCs, TILs and tumor cell.


Subject(s)
Dendritic Cells/pathology , Rectal Neoplasms/pathology , T-Lymphocytes/pathology , Basement Membrane/pathology , Basement Membrane/ultrastructure , Cytotoxicity, Immunologic , Dendritic Cells/ultrastructure , Disease Progression , Glycogen/analysis , Humans , Immunohistochemistry , Microscopy, Electron, Transmission , Neoplasm Staging , Rectal Neoplasms/chemistry , Rectal Neoplasms/ultrastructure , T-Lymphocytes/ultrastructure , T-Lymphocytes, Cytotoxic/pathology
8.
J Clin Pathol ; 59(10): 1039-43, 2006 Oct.
Article in English | MEDLINE | ID: mdl-16467161

ABSTRACT

BACKGROUND: The introduction of preoperative chemoradiation into the treatment protocol of rectal adenocarcinomas has affected the microscopical morphology in subsequent resection specimens. The constellation of histopathological changes is varied and well documented. AIM: To describe oncocytic change in rectal cancers that have been treated with chemoradiation before surgery. METHODS: 7 of 54 patients with rectal cancer were identified with a history of chemoradiation, specifically directed to the rectal tumours in fractions of 4500-5000 cGy of radiation and 5-fluorouracil. The rectal tumours in five of these seven patients were composed of oncocytes that constituted 30-80% of the cancers. The patients were three men and two women aged 65-73 years, all with T3 N0 tumours. The intervals between chemoradiation and resection varied from 3 to 12 weeks. RESULTS: The tumour cells conformed to oncocytes morphologically (large size with abundant, granular eosinophilic cytoplasm, vesicular nuclei and prominent acidophilic nucleoli), immunohistochemically (positive for carcinoembryonic antigen, cytokeratin 20 and caudal type homeo box transcription factor 2, but negative for both chromogranin and synaptophysin) and ultrastructurally (large cells showing tight junctions, cytoplasmic engorgement by mitochondria and absence of neurosecretory granules). CONCLUSIONS: The changes in these cells differ from those described previously in endocrine cells encountered in pretreated rectal cancers. Oncocytic change in this particular clinical context occurs as a reflection of cytotoxic damage or cellular hypoxia induced by chemoradiation resulting in degeneration of the cell and the oncocytic phenotype. Oncocytic change may be an under-recognised histopathological change in rectal cancers receiving preoperative chemoradiation.


Subject(s)
Adenocarcinoma/ultrastructure , Oxyphil Cells/ultrastructure , Rectal Neoplasms/ultrastructure , Adenocarcinoma/therapy , Aged , Antimetabolites, Antineoplastic/therapeutic use , Biomarkers, Tumor/analysis , Chemotherapy, Adjuvant , Female , Fluorouracil/therapeutic use , Follow-Up Studies , Humans , Male , Neoadjuvant Therapy , Radiotherapy, Adjuvant , Rectal Neoplasms/therapy
9.
Histopathology ; 44(3): 247-50, 2004 Mar.
Article in English | MEDLINE | ID: mdl-14987228

ABSTRACT

AIMS: To examine whether or not the tight junction-associated transmembrane protein occludin is expressed in rosette or gland-like structures in human rectal carcinoid tumours. The tight junction is crucial for the formation and maintenance of organized tubular structures in glandular epithelia. Previous studies have reported the presence of glandular structures in carcinoid tumours, though they are not believed to arise from glandular epithelium. METHODS AND RESULTS: The expression profiles of occludin in 40 carcinoid tumours were examined immunohistochemically, using an anti-occludin monoclonal antibody. In eight (20%) samples of typical carcinoid tumours, a small number of rosette-like tubular structures outlined by occludin were detected. CONCLUSIONS: Tight junction-associated molecules, including occludin, are thought to be one of the most characteristic structural markers of polarized glandular structures. The results of the present study provide supportive evidence that carcinoid tumour cells are capable of glandular differentiation.


Subject(s)
Carcinoid Tumor/pathology , Membrane Proteins/biosynthesis , Rectal Neoplasms/pathology , Tight Junctions/ultrastructure , Adult , Aged , Aged, 80 and over , Carcinoid Tumor/metabolism , Carcinoid Tumor/ultrastructure , Cell Differentiation , Female , Humans , Immunohistochemistry , Male , Middle Aged , Occludin , Rectal Neoplasms/metabolism , Rectal Neoplasms/ultrastructure
10.
Di Yi Jun Yi Da Xue Xue Bao ; 24(1): 79-80, 84, 2004 Jan.
Article in Chinese | MEDLINE | ID: mdl-14724105

ABSTRACT

OBJECTIVE: To observe the microscopic characteristics of laterally spreading tumor (LST) cell line in primary culture. METHODS: The cells isolated from a rectum LST specimen obtained by endoscopic mucosal resection was primary cultured, followed by observation with scanning and transmission electron microscope in comparison with the cells of adenocarcinoma and normal mucosa of the rectum. RESULTS: Scanning and transmission electron microscopes both revealed numerous microvilli covering the surface of the LST cells, and the cytoplasm contained large quantity of lysosomes, mitochondria and phagosomes. Obviously heterogeneous cell nuclei were present with abnormal nuclear fossa and huge nucleoli. CONCLUSION: The cultured LST cells are highly malignant.


Subject(s)
Adenocarcinoma/ultrastructure , Rectal Neoplasms/ultrastructure , Cell Line, Tumor , Humans , Microscopy, Electron
11.
Virchows Arch ; 443(4): 579-82, 2003 Oct.
Article in English | MEDLINE | ID: mdl-12955503

ABSTRACT

Several histological variants of colorectal carcinoma have been reported, some of them bearing prognostic significance, others only incidental findings showing unusual morphological features. The current report was aimed to describe the histological, immunohistochemical and ultrastructural features of an oncocytic adenocarcinoma of the rectum occurring in a 66-year-old woman. Histologically, it was a moderately differentiated adenocarcinoma composed by glandular structures lined by eosinophilic cells. The latter showed abundant granular cytoplasm and large nuclei with prominent nucleoli. Several glandular structures contained intraluminal, basophilic and non-birifrangent microcalcifications. The tumour cells displayed consistent anti-mitochondrial antigen, carcinoembryonic antigen, p53, CDX2 and cytokeratin 20 immunoreactivity. Ultrastructurally, more than 80% of the cytoplasmic area was occupied by abnormal mitochondria, while exocrine or endocrine granules were undetectable. The tumour infiltrated the intestinal wall through the subserosal tissue, but lymph node or distant metastases were absent. The patient is disease free 22 months after surgery. Based on the above features, this case could be appropriately named oncocytic adenocarcinoma with intraluminal microcalcifications. Like gastric neoplasms showing similar morphologic features, this tumour might have a better prognosis, and the presence of microcalcifcations could help its proper recognition at a pre-operative stage.


Subject(s)
Adenocarcinoma/pathology , Calcinosis/pathology , Rectal Neoplasms/pathology , Adenocarcinoma/chemistry , Adenocarcinoma/ultrastructure , Aged , Female , Humans , Immunohistochemistry , Rectal Neoplasms/chemistry , Rectal Neoplasms/ultrastructure
12.
Anal Quant Cytol Histol ; 25(4): 215-20, 2003 Aug.
Article in English | MEDLINE | ID: mdl-12961828

ABSTRACT

OBJECTIVE: To investigate the prognostic value of nuclear features in rectal carcinoma. STUDY DESIGN: High-resolution imagery of 3,635 nuclei from 51 patients operated on for rectal cancer at various Dukes' stages was digitally recorded. A set of 93 features descriptive of the spatial and statistical distribution of nuclear chromatin was computed for each nucleus to derive a digital signature. Karyometric features were analyzed for correlation with progression of disease and death. RESULTS: Multivariate analysis of main karyometric features in comparison with cancer staging demonstrated that total optical density and clumpness, as well as average nuclear signature, had significant prognostic value in predicting cancer-related death. CONCLUSION: Digital signature seems to have a role as prognostic factor in rectal cancer. The method could be a useful parameter in deciding whether to perform adjuvant therapy in particular subgroups of patients, independently of tumor staging. However, these observations need to be substantiated with additional studies, including larger numbers of patients.


Subject(s)
Carcinoma/ultrastructure , Chromatin/ultrastructure , Cytogenetic Analysis/methods , Rectal Neoplasms/ultrastructure , Adult , Aged , Carcinoma/mortality , Female , Humans , Male , Middle Aged , Prognosis , Rectal Neoplasms/mortality , Survival Analysis
13.
Ann Surg ; 233(6): 778-85, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11371736

ABSTRACT

OBJECTIVE: To examine clinical outcomes in patients receiving neoadjuvant chemoradiation for locally advanced rectal adenocarcinoma. SUMMARY BACKGROUND DATA: Preoperative radiation therapy, either alone or in combination with 5-fluorouracil-based chemotherapy, has proven both safe and effective in the treatment of rectal cancer. However, data are lacking regarding which subgroups of patients benefit from the therapy in terms of decreased local recurrence and increased survival rates. METHODS: A retrospective chart review was performed on 141 consecutive patients who received neoadjuvant chemoradiation (5-fluorouracil +/- cisplatin and 4,500-5,040 cGy) for biopsy-proven locally advanced adenocarcinoma of the rectum. Surgery was performed 4 to 8 weeks after completion of chemoradiation. Standard statistical methods were used to analyze recurrence and survival. RESULTS: Median follow-up was 27 months, and mean age was 59 years (range 28-81). Mean tumor distance from the anal verge was 6 cm (range 1-15). Of those staged before surgery with endorectal ultrasound or magnetic resonance imaging, 57% of stage II patients and 82% of stage III patients were downstaged. The chemotherapeutic regimens were well tolerated, and resections were performed on 140 patients. The percentage of sphincter-sparing procedures increased from 20% before 1996 to 76% after 1996. On pathologic analysis, 24% of specimens were T0. However, postoperative pathologic T stage had no effect on either recurrence or survival. Positive lymph node status predicted increased local recurrence and decreased survival. CONCLUSIONS: Neoadjuvant chemoradiation is safe, effective, and well tolerated. Postoperative lymph node status is the only independent predictor of recurrence and survival.


Subject(s)
Adenocarcinoma/radiotherapy , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Preoperative Care , Rectal Neoplasms/radiotherapy , Adenocarcinoma/pathology , Adenocarcinoma/ultrastructure , Adult , Aged , Aged, 80 and over , Cisplatin/administration & dosage , Combined Modality Therapy , Digestive System Surgical Procedures , Female , Fluorouracil/administration & dosage , Humans , Magnetic Resonance Imaging , Male , Middle Aged , Prognosis , Rectal Neoplasms/pathology , Rectal Neoplasms/ultrastructure , Regression Analysis , Retrospective Studies , Treatment Outcome
14.
Pathol Oncol Res ; 5(3): 205-10, 1999.
Article in English | MEDLINE | ID: mdl-10491018

ABSTRACT

Intestinal carcinoids are potentially malignant neoplasms. Their histogenesis and pathogenesis are currently uncertain. The morphological and histochemical characteristics of twenty intestinal carcinoids are studied. The primary sites of three mucin-producing tumors were examined by electron microscope. Furthermore 11 appendiceal carcinoids were analysed by the polymerase chain reaction (PCR) for the detection of ras and p53 point mutations. Microscopically all carcinoids were of mixed type. Focal mucin production was evident in three carcinoids that metastasised to regional lymph nodes. HID-Alcian blue staining proved that mucin in both primary and secondary foci did not belong to the sulphated group. The secretory granules and mucin droplets found in a single neoplastic cell suggest that carcinoids of the small intestine and some of the appendix arise from the endoderm. Neither ras nor p53 mutations were detected. It seems that ras oncogenes are probably not involved in the pathogenesis of appendiceal carcinoids.


Subject(s)
Appendiceal Neoplasms/pathology , Carcinoid Tumor/pathology , Intestinal Neoplasms/pathology , ras Proteins/metabolism , Adolescent , Adult , Aged , Aged, 80 and over , Appendiceal Neoplasms/genetics , Appendiceal Neoplasms/metabolism , Carcinoid Tumor/genetics , Carcinoid Tumor/metabolism , Child , DNA Mutational Analysis , Female , Humans , Ileal Neoplasms/genetics , Ileal Neoplasms/pathology , Ileal Neoplasms/ultrastructure , Intestinal Neoplasms/genetics , Intestinal Neoplasms/metabolism , Male , Microscopy, Electron , Middle Aged , Mucins/metabolism , Mutation , Polymerase Chain Reaction , Rectal Neoplasms/genetics , Rectal Neoplasms/pathology , Rectal Neoplasms/ultrastructure , Tumor Suppressor Protein p53/genetics , Tumor Suppressor Protein p53/metabolism , ras Proteins/genetics
15.
Am J Surg Pathol ; 23(7): 838-43, 1999 Jul.
Article in English | MEDLINE | ID: mdl-10403308

ABSTRACT

A 62-year-old man with a history of a resected rectal polyp was diagnosed 14 years later with right liver and multiple bone metastases. The liver biopsy showed a malignant epithelial tumor that was positive for neuron-specific enolase immunostaining and negative for chromogranin. Electron microscopy was characteristic of that for an endocrine tumor. Most circulating hormonal peptide levels were within normal ranges and only motilin level was elevated. On the right hepatectomy, the three large metastases had a histologic picture suggestive of an endocrine tumor. Immunohistochemistry revealed in some areas numerous tumor cells expressing motilin, and a few cells were strongly positive for pancreatic polypeptide and somatostatin. The retrospective analysis of the rectal polyp showed a similar histology and immunohistochemical profile, indicating that this lesion was the primary tumor. Motilin-positive cells from one of the hepatic lesions were identified on semithin sections and further processed for electron microscopy. Neurosecretory granules were numerous in all cells. Immunoelectron localization enabled us to characterize the motilin-containing neurosecretory granules, which had a mean diameter of 168.3x38.1 nm. Although not all tumor cells were motilin-positive, a diagnosis of motilinoma for the rectal polyp and its hepatic and bone metastases was proposed.


Subject(s)
Bone Neoplasms/metabolism , Carcinoid Tumor/metabolism , Liver Neoplasms/metabolism , Motilin/biosynthesis , Polyps/metabolism , Rectal Neoplasms/metabolism , Biopsy , Bone Neoplasms/pathology , Bone Neoplasms/secondary , Bone Neoplasms/ultrastructure , Carcinoid Tumor/pathology , Carcinoid Tumor/secondary , Carcinoid Tumor/surgery , Carcinoid Tumor/ultrastructure , Cytoplasmic Granules/metabolism , Cytoplasmic Granules/ultrastructure , Humans , Immunohistochemistry , Liver Neoplasms/pathology , Liver Neoplasms/secondary , Liver Neoplasms/ultrastructure , Male , Microscopy, Immunoelectron , Middle Aged , Polyps/pathology , Polyps/surgery , Polyps/ultrastructure , Rectal Neoplasms/pathology , Rectal Neoplasms/surgery , Rectal Neoplasms/ultrastructure , Time
16.
Dis Colon Rectum ; 42(3): 386-92, 1999 Mar.
Article in English | MEDLINE | ID: mdl-10223762

ABSTRACT

PURPOSE: In many types of cancer, certain morphometric characteristic of tumor cells correlate with patient survival. Our observations suggested that the survival of patients with colorectal carcinomas is negatively correlated with tumor-cell nucleus size. METHODS: We investigated relationships between postsurgery survival and nucleus morphometrics in 90 patients who had undergone resection for a colorectal tumor. The nucleus-size variables considered were maximum diameter, minimum diameter, perimeter, area, and form factor (means for 100 nuclei from each patient were used in all cases). RESULTS: Our results confirmed that patients with large maximum nucleus diameter (where large = greater than the first quartile) have significantly worse survival than patients with smaller maximum nucleus diameter (mean survival, 28 vs. 43 months). Similar results were obtained for the other nucleus-size variables. Stepwise Cox regression analysis was then performed, with postsurgery survival time as the dependent variable and the following candidate independent variables: age, gender, Dukes class, degree of histologic differentiation, the various nucleus-size variables, and relative frequencies of different nucleus shapes (spherical, oval, cylindrical, fusiform, and irregular). The variables selected for the prognostic model were Dukes class, relative frequency of irregular nuclei, and maximum nucleus diameter. CONCLUSIONS: These findings indicated that nucleus size and shape are useful predictors of survival. Even if Dukes class is known, consideration of nucleus size and shape significantly improves prediction of survival.


Subject(s)
Cell Nucleus/ultrastructure , Colonic Neoplasms/ultrastructure , Intestinal Mucosa/ultrastructure , Rectal Neoplasms/ultrastructure , Colonic Neoplasms/mortality , Colonic Neoplasms/pathology , Humans , Intestinal Mucosa/pathology , Prognosis , Proportional Hazards Models , Rectal Neoplasms/mortality , Rectal Neoplasms/pathology , Regression Analysis , Survival Analysis
17.
Ultrastruct Pathol ; 22(5): 393-400, 1998.
Article in English | MEDLINE | ID: mdl-9887482

ABSTRACT

Samples from rectal plasmacytoma in three adult dogs that were diagnosed by light microscopy and immunohistochemistry were examined by electron microscopy. The most common cell type had typical plasmacytoid features. A second cell type was a plasmacytoid giant cell with single or multiple eccentric nuclei, irregular nuclear membrane, abundant and dilated rough endoplasmic reticulum, and numerous electron-dense granules. The third cell type was a histiocytic giant cell that intermingled with plasmacytoid cells. All three tumors had abundant amyloid, mainly in the interstitium but also within histiocytic cells and less commonly in plasma cells or plasmacytoid giant cells. Extracellular and intracellular amyloid fibrils and the contents of membrane-bound electron-dense bodies of plasma cells reacted with antibody to lambda-light chain of immunoglobulins by immunogold staining.


Subject(s)
Amyloid/metabolism , Dog Diseases/pathology , Microscopy, Immunoelectron/veterinary , Plasmacytoma/veterinary , Rectal Neoplasms/veterinary , Amyloid/ultrastructure , Animals , Dog Diseases/metabolism , Dogs , Organelles/ultrastructure , Plasma Cells/ultrastructure , Plasmacytoma/metabolism , Plasmacytoma/ultrastructure , Rectal Neoplasms/metabolism , Rectal Neoplasms/ultrastructure
18.
G Chir ; 18(8-9): 433-6, 1997.
Article in Italian | MEDLINE | ID: mdl-9471221

ABSTRACT

The Authors report a case of recurrent leiomyoma of the rectum treated by Transanal Endoscopic Microsurgery (T.E.M.). Leiomyoma of the rectum is a rare entity (0.1-0.3%) (the incidence of smooth muscle tumours being 7% in the digestive tract). Benign leiomyomas are usually asymptomatic; discomfort or pain, related or not to defecation, sensation of foreign body, change in bowel habits, rectal bleeding are rarely reported. The distinction between a benign leiomyoma and a leiomyosarcoma is often difficult and requires an accurate microscopic study. In most cases rectal leiomyoma is detected incidentally in the course of a rectal examination. Endoscopic examination of the rectum with biopsies and endorectal ultrasonography are useful for the diagnosis, while rarely a plain radiologic examination is sufficient. Leiomyoma of the rectum also presents a high tendency to local recurrence (31%). Therefore the choice of an adequate treatment is often difficult. The Authors believe that the treatment of rectal leiomyoma by T.E.M. may substitute conventional methodics (transanal excision, proctectomy with or without amputation of the sphincter and coloanal anastomosis, endoscopic electroexcision of the neoplasm). T.E.M. allows short-term hospitalization and implies minimal surgical trauma.


Subject(s)
Leiomyoma/surgery , Rectal Neoplasms/surgery , Anal Canal , Female , Humans , Intestinal Mucosa/ultrastructure , Leiomyoma/diagnostic imaging , Leiomyoma/ultrastructure , Microsurgery/methods , Middle Aged , Rectal Neoplasms/diagnostic imaging , Rectal Neoplasms/ultrastructure , Ultrasonography
19.
Arkh Patol ; 59(1): 37-41, 1997.
Article in Russian | MEDLINE | ID: mdl-9139606

ABSTRACT

Results of combined treatment of 113 patients with colon carcinoma receiving a single irradiation (7.5 Gy) before the operation were analysed. The single irradiation was well tolerated by all the patients without complications during the operation and in the postoperative period. It is found at the ultrastructural level that from 70 to 85% of the cells in the tumors removed were irreversibly damaged.


Subject(s)
Preoperative Care/methods , Rectal Neoplasms/ultrastructure , Combined Modality Therapy , Dose-Response Relationship, Radiation , Humans , Microcirculation/physiology , Microscopy, Electron , Radiation Tolerance , Rectal Neoplasms/radiotherapy , Rectal Neoplasms/surgery
20.
South Med J ; 89(9): 921-4, 1996 Sep.
Article in English | MEDLINE | ID: mdl-8790320

ABSTRACT

Primary small cell undifferentiated carcinomas (SCUCs) are unusual tumors of the colon and rectum. Histologically, these lesions represent a spectrum of neuroendocrine differentiation, with oat cell carcinoma being the most primitive subtype and carcinoid tumors being the most differentiated. This observation is supported by immunohistochemical and ultrastructural findings. We report a case of SCUC of the rectum in a patient with ulcerative colitis. To date, there have been only two reported cases of primary SCUC associated with ulcerative colitis. Recent theories of tumorigenesis attribute most colorectal cancers to a single, pluripotential mucosal stem cell, regardless of the tumor's histologic type.


Subject(s)
Carcinoma, Small Cell/complications , Colitis, Ulcerative/complications , Rectal Neoplasms/complications , Carcinoma, Neuroendocrine/complications , Carcinoma, Neuroendocrine/secondary , Carcinoma, Neuroendocrine/ultrastructure , Carcinoma, Small Cell/secondary , Carcinoma, Small Cell/ultrastructure , Colitis, Ulcerative/pathology , Fatal Outcome , Female , Humans , Immunohistochemistry , Intestinal Mucosa/pathology , Keratins/analysis , Middle Aged , Neoplastic Stem Cells/pathology , Phosphopyruvate Hydratase/analysis , Rectal Neoplasms/ultrastructure , Rectum/pathology , Synaptophysin/analysis , Tumor Suppressor Protein p53/analysis
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