ABSTRACT
PURPOSE: The aim of the study was to assess the occurrence of human papillomavirus (HPV) DNA in pterygium. METHODS: The study involved 89 patients undergoing surgical procedures at the Department of Ophthalmology, Medical University of Lublin, Poland. Group 1 included 58 patients with clinically diagnosed pterygium. Group 2 consisted of 31 individuals with normal conjunctiva. The material was collected during elective surgical procedures. The presence of HPV genome was determined using polymerase chain reaction (PCR). Once the presence of HPV DNA was confirmed, 28 HPV genotypes were determined using reverse hybridization. RESULTS: The determinations confirmed the presence of HPV DNA in pterygium. In the material collected from 58 cases of pterygium (group 1), HPV DNA was identified in 16 patients (27.6%). In the material from 31 diagnostic specimens of normal conjunctiva (group 2), the presence of HPV was demonstrated in three cases (9.7%). A statistically significant difference was found in the presence of HPV DNA between the patients from groups 1 and 2 (p = 0.041). HPV type 16 was most common and was demonstrated in 56% of HPV-positive cases of pterygium. HPV 16 and HPV 6 co-infections were found in 19% of cases, while HPV 18 and HPV 6 co-infections were observed in 13%. In group 2, all three patients with HPV showed HPV 18. CONCLUSION: It seems that HPV is not necessary to induce pterygium; however, it might play a synergistic role in the multi-stage process of its development.
Subject(s)
Human papillomavirus 16 , Human papillomavirus 18 , Human papillomavirus 6 , Papillomavirus Infections/epidemiology , Pterygium/virology , Adult , Aged , Aged, 80 and over , Conjunctiva/metabolism , Conjunctiva/virology , DNA, Viral/metabolism , Female , Human papillomavirus 16/genetics , Human papillomavirus 18/genetics , Human papillomavirus 6/genetics , Humans , Incidence , Male , Middle Aged , Polymerase Chain Reaction , Pterygium/metabolism , Retinal Detachment/metabolism , Retinal Detachment/virology , Retinal Perforations/metabolism , Retinal Perforations/virology , Strabismus/metabolism , Strabismus/virology , Young AdultABSTRACT
OBJECTIVE: To evaluate the efficacy and results of pars plana vitrectomy with endotamponade for retinal detachments caused by necrotizing retinitis in HIV patients. MATERIAL AND METHOD: The data of patients with HIV-related retinal detachment who underwent pars plana vitrectomy with silicone oil or gas endotamponade between January 2003 and June 2005 were retrospectively reviewed The outcome measures were demographic data, anatomical, and visual results. RESULTS: Of all 24 eyes from 20 patients, 19 eyes underwent pars plana vitrectomy with silicone oil tamponade and 5 eyes with long-acting gas tamponade. Mean follow up time was 13 months (range 2-33 months). The overall anatomical success was 83% (84% and 80% with silicone oil and gas tamponade, respectively). Final best corrected visual acuity was equal or better than 5/200 in 12 eyes (50%). Forty-six percent had stabled or improved vision at the end of follow-up. CONCLUSION: Pars plana vitrectomy with silicone oil or gas tamponade gives the high anatomical success rate in the repair of retinal detachments caused by necrotizing retinitis in HIV patients. There were the same reattachment rate and visual results between the two tamponade groups. However, the use of gas tamponade may be effective in patients with highly active antiretroviral therapy (HAART).
