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1.
Nature ; 613(7945): 775-782, 2023 01.
Article in English | MEDLINE | ID: mdl-36442503

ABSTRACT

CRISPR-associated transposons (CAST) are programmable mobile genetic elements that insert large DNA cargos using an RNA-guided mechanism1-3. CAST elements contain multiple conserved proteins: a CRISPR effector (Cas12k or Cascade), a AAA+ regulator (TnsC), a transposase (TnsA-TnsB) and a target-site-associated factor (TniQ). These components are thought to cooperatively integrate DNA via formation of a multisubunit transposition integration complex (transpososome). Here we reconstituted the approximately 1 MDa type V-K CAST transpososome from Scytonema hofmannii (ShCAST) and determined its structure using single-particle cryo-electon microscopy. The architecture of this transpososome reveals modular association between the components. Cas12k forms a complex with ribosomal subunit S15 and TniQ, stabilizing formation of a full R-loop. TnsC has dedicated interaction interfaces with TniQ and TnsB. Of note, we observe TnsC-TnsB interactions at the C-terminal face of TnsC, which contribute to the stimulation of ATPase activity. Although the TnsC oligomeric assembly deviates slightly from the helical configuration found in isolation, the TnsC-bound target DNA conformation differs markedly in the transpososome. As a consequence, TnsC makes new protein-DNA interactions throughout the transpososome that are important for transposition activity. Finally, we identify two distinct transpososome populations that differ in their DNA contacts near TniQ. This suggests that associations with the CRISPR effector can be flexible. This ShCAST transpososome structure enhances our understanding of CAST transposition systems and suggests ways to improve CAST transposition for precision genome-editing applications.


Subject(s)
CRISPR-Cas Systems , DNA Transposable Elements , Gene Editing , Holoenzymes , Multiprotein Complexes , RNA, Guide, CRISPR-Cas Systems , Transposases , Clustered Regularly Interspaced Short Palindromic Repeats/genetics , DNA Transposable Elements/genetics , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/metabolism , DNA-Binding Proteins/ultrastructure , Gene Editing/methods , Transposases/chemistry , Transposases/metabolism , Transposases/ultrastructure , RNA, Guide, CRISPR-Cas Systems/genetics , Holoenzymes/chemistry , Holoenzymes/metabolism , Holoenzymes/ultrastructure , Multiprotein Complexes/chemistry , Multiprotein Complexes/metabolism , Multiprotein Complexes/ultrastructure , Cryoelectron Microscopy , Ribosome Subunits/chemistry , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure , Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Bacterial Proteins/ultrastructure
2.
Mol Cell ; 82(4): 756-769.e8, 2022 02 17.
Article in English | MEDLINE | ID: mdl-35120588

ABSTRACT

The superkiller (SKI) complex is the cytoplasmic co-factor and regulator of the RNA-degrading exosome. In human cells, the SKI complex functions mainly in co-translational surveillance-decay pathways, and its malfunction is linked to a severe congenital disorder, the trichohepatoenteric syndrome. To obtain insights into the molecular mechanisms regulating the human SKI (hSKI) complex, we structurally characterized several of its functional states in the context of 80S ribosomes and substrate RNA. In a prehydrolytic ATP form, the hSKI complex exhibits a closed conformation with an inherent gating system that effectively traps the 80S-bound RNA into the hSKI2 helicase subunit. When active, hSKI switches to an open conformation in which the gating is released and the RNA 3' end exits the helicase. The emerging picture is that the gatekeeping mechanism and architectural remodeling of hSKI underpin a regulated RNA channeling system that is mechanistically conserved among the cytoplasmic and nuclear helicase-exosome complexes.


Subject(s)
Exoribonucleases/metabolism , Exosome Multienzyme Ribonuclease Complex/metabolism , RNA Helicases/metabolism , RNA Processing, Post-Transcriptional , RNA Stability , RNA/metabolism , Ribosome Subunits/metabolism , Adenosine Triphosphate/metabolism , Binding Sites , Exoribonucleases/genetics , Exoribonucleases/ultrastructure , Exosome Multienzyme Ribonuclease Complex/genetics , Exosome Multienzyme Ribonuclease Complex/ultrastructure , HEK293 Cells , Humans , Models, Molecular , Nucleic Acid Conformation , Protein Conformation , RNA/genetics , RNA/ultrastructure , RNA Helicases/genetics , RNA Helicases/ultrastructure , Ribosome Subunits/genetics , Ribosome Subunits/ultrastructure , Structure-Activity Relationship
3.
RNA ; 27(9): 981-990, 2021 09.
Article in English | MEDLINE | ID: mdl-34117118

ABSTRACT

Many antibiotics that bind to the ribosome inhibit translation by blocking the movement of tRNAs and mRNA or interfering with ribosome dynamics, which impairs the formation of essential translocation intermediates. Here we show how translocation inhibitors viomycin (Vio), neomycin (Neo), paromomycin (Par), kanamycin (Kan), spectinomycin (Spc), hygromycin B (HygB), and streptomycin (Str, an antibiotic that does not inhibit tRNA movement), affect principal motions of the small ribosomal subunits (SSU) during EF-G-promoted translocation. Using ensemble kinetics, we studied the SSU body domain rotation and SSU head domain swiveling in real time. We show that although antibiotics binding to the ribosome can favor a particular ribosome conformation in the absence of EF-G, their kinetic effect on the EF-G-induced transition to the rotated/swiveled state of the SSU is moderate. The antibiotics mostly inhibit backward movements of the SSU body and/or the head domains. Vio, Spc, and high concentrations of Neo completely inhibit the backward movements of the SSU body and head domain. Kan, Par, HygB, and low concentrations of Neo slow down both movements, but their sequence and coordination are retained. Finally, Str has very little effect on the backward rotation of the SSU body domain, but retards the SSU head movement. The data underscore the importance of ribosome dynamics for tRNA-mRNA translocation and provide new insights into the mechanism of antibiotic action.


Subject(s)
Anti-Bacterial Agents/pharmacology , Escherichia coli/drug effects , Protein Biosynthesis/drug effects , RNA, Messenger/metabolism , RNA, Transfer/metabolism , Ribosome Subunits/drug effects , Biological Transport , Cinnamates/pharmacology , Escherichia coli/genetics , Escherichia coli/metabolism , Hygromycin B/analogs & derivatives , Hygromycin B/pharmacology , Kanamycin/pharmacology , Kinetics , Neomycin/pharmacology , Paromomycin/pharmacology , Peptide Elongation Factor G/genetics , Peptide Elongation Factor G/metabolism , RNA, Messenger/chemistry , RNA, Messenger/genetics , RNA, Transfer/antagonists & inhibitors , RNA, Transfer/chemistry , RNA, Transfer/genetics , Ribosome Subunits/genetics , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure , Spectinomycin/pharmacology , Streptomycin/pharmacology , Viomycin/pharmacology
4.
Elife ; 92020 04 14.
Article in English | MEDLINE | ID: mdl-32286223

ABSTRACT

Taking control of the cellular apparatus for protein production is a requirement for virus progression. To ensure this control, diverse strategies of cellular mimicry and/or ribosome hijacking have evolved. The initiation stage of translation is especially targeted as it involves multiple steps and the engagement of numerous initiation factors. The use of structured RNA sequences, called Internal Ribosomal Entry Sites (IRES), in viral RNAs is a widespread strategy for the exploitation of eukaryotic initiation. Using a combination of electron cryo-microscopy (cryo-EM) and reconstituted translation initiation assays with native components, we characterized how a novel IRES at the 5'-UTR of a viral RNA assembles a functional initiation complex via an uAUG intermediate. The IRES features a novel extended, multi-domain architecture, that circles the 40S head. The structures and accompanying functional data illustrate the importance of 5'-UTR regions in translation regulation and underline the relevance of the untapped diversity of viral IRESs.


Subject(s)
Dicistroviridae , Eukaryotic Initiation Factor-3/ultrastructure , Internal Ribosome Entry Sites , Models, Molecular , RNA, Viral/ultrastructure , 5' Untranslated Regions , Animals , Cryoelectron Microscopy , Eukaryotic Initiation Factor-3/chemistry , Eukaryotic Initiation Factor-3/metabolism , Humans , Protein Biosynthesis/physiology , Protein Conformation , RNA, Messenger/chemistry , RNA, Messenger/metabolism , RNA, Messenger/ultrastructure , RNA, Viral/chemistry , RNA, Viral/metabolism , Ribosome Subunits/chemistry , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure
5.
Nat Methods ; 16(8): 757-762, 2019 08.
Article in English | MEDLINE | ID: mdl-31363205

ABSTRACT

Cryo-focused ion beam milling of frozen-hydrated cells has recently provided unprecedented insights into the inner space of cells. In combination with cryo-electron tomography, this method allows access to native structures deep inside cells, enabling structural studies of macromolecules in situ. However, this approach has been mainly limited to individual cells that can be completely vitrified by plunge-freezing. Here, we describe a preparation method that is based on the targeted extraction of material from high-pressure-frozen bulk specimens with a cryo-gripper tool. This lift-out technique enables cryo-electron tomography to be performed on multicellular organisms and tissue, extending the range of applications for in situ structural biology. We demonstrate the potential of the lift-out technique with a structural study of cytosolic 80S ribosomes in a Caenorhabditis elegans worm. The preparation quality allowed for subtomogram analysis with sufficient resolution to distinguish individual ribosomal translocation states and revealed significant cell-to-cell variation in ribosome structure.


Subject(s)
Caenorhabditis elegans/ultrastructure , Cryoelectron Microscopy/methods , Electron Microscope Tomography/methods , Macromolecular Substances/ultrastructure , Ribosome Subunits/ultrastructure , Animals
6.
RNA ; 25(5): 600-606, 2019 05.
Article in English | MEDLINE | ID: mdl-30733327

ABSTRACT

The 70S ribosome is a major target for antibacterial drugs. Two of the classical antibiotics, chloramphenicol (CHL) and erythromycin (ERY), competitively bind to adjacent but separate sites on the bacterial ribosome: the catalytic peptidyl transferase center (PTC) and the nascent polypeptide exit tunnel (NPET), respectively. The previously reported competitive binding of CHL and ERY might be due either to a direct collision of the two drugs on the ribosome or due to a drug-induced allosteric effect. Because of the resolution limitations, the available structures of these antibiotics in complex with bacterial ribosomes do not allow us to discriminate between these two possible mechanisms. In this work, we have obtained two crystal structures of CHL and ERY in complex with the Thermus thermophilus 70S ribosome at a higher resolution (2.65 and 2.89 Å, respectively) allowing unambiguous placement of the drugs in the electron density maps. Our structures provide evidence of the direct collision of CHL and ERY on the ribosome, which rationalizes the observed competition between the two drugs.


Subject(s)
Anti-Bacterial Agents/chemistry , Chloramphenicol/chemistry , Erythromycin/chemistry , Ribosome Subunits/drug effects , Thermus thermophilus/drug effects , Anti-Bacterial Agents/pharmacology , Binding Sites , Binding, Competitive , Chloramphenicol/pharmacology , Crystallography, X-Ray , Erythromycin/pharmacology , Escherichia coli/chemistry , Escherichia coli/drug effects , Escherichia coli/genetics , Escherichia coli/metabolism , Models, Molecular , Peptidyl Transferases/antagonists & inhibitors , Peptidyl Transferases/chemistry , Peptidyl Transferases/genetics , Peptidyl Transferases/metabolism , Protein Binding , Protein Biosynthesis , Protein Conformation , Ribosome Subunits/genetics , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure , Thermus thermophilus/chemistry , Thermus thermophilus/genetics , Thermus thermophilus/metabolism
7.
Nature ; 564(7736): 444-448, 2018 12.
Article in English | MEDLINE | ID: mdl-30518861

ABSTRACT

Orthogonal ribosomes are unnatural ribosomes that are directed towards orthogonal messenger RNAs in Escherichia coli, through an altered version of the 16S ribosomal RNA of the small subunit1. Directed evolution of orthogonal ribosomes has provided access to new ribosomal function, and the evolved orthogonal ribosomes have enabled the encoding of multiple non-canonical amino acids into proteins2-4. The original orthogonal ribosomes shared the pool of 23S ribosomal RNAs, contained in the large subunit, with endogenous ribosomes. Selectively directing a new 23S rRNA to an orthogonal mRNA, by controlling the association between the orthogonal 16S rRNAs and 23S rRNAs, would enable the evolution of new function in the large subunit. Previous work covalently linked orthogonal 16S rRNA and a circularly permuted 23S rRNA to create orthogonal ribosomes with low activity5,6; however, the linked subunits in these ribosomes do not associate specifically with each other, and mediate translation by associating with endogenous subunits. Here we discover engineered orthogonal 'stapled' ribosomes (with subunits linked through an optimized RNA staple) with activities comparable to that of the parent orthogonal ribosome; they minimize association with endogenous subunits and mediate translation of orthogonal mRNAs through the association of stapled subunits. We evolve cells with genomically encoded stapled ribosomes as the sole ribosomes, which support cellular growth at similar rates to natural ribosomes. Moreover, we visualize the engineered stapled ribosome structure by cryo-electron microscopy at 3.0 Å, revealing how the staple links the subunits and controls their association. We demonstrate the utility of controlling subunit association by evolving orthogonal stapled ribosomes which efficiently polymerize a sequence of monomers that the natural ribosome is intrinsically unable to translate. Our work provides a foundation for evolving the rRNA of the entire orthogonal ribosome for the encoded cellular synthesis of non-canonical biological polymers7.


Subject(s)
Directed Molecular Evolution , Escherichia coli , Protein Biosynthesis , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure , Ribosomes/metabolism , Ribosomes/ultrastructure , Base Sequence , Cross-Linking Reagents/chemistry , Cryoelectron Microscopy , Escherichia coli/classification , Escherichia coli/cytology , Escherichia coli/genetics , Escherichia coli/growth & development , Models, Molecular , Peptides/genetics , Peptides/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Ribosomal, 16S/chemistry , RNA, Ribosomal, 16S/genetics , RNA, Ribosomal, 16S/metabolism , RNA, Ribosomal, 16S/ultrastructure , RNA, Ribosomal, 23S/chemistry , RNA, Ribosomal, 23S/genetics , RNA, Ribosomal, 23S/metabolism , RNA, Ribosomal, 23S/ultrastructure , Ribosome Subunits/chemistry , Ribosomes/chemistry , Ribosomes/genetics
8.
Nat Methods ; 15(11): 955-961, 2018 11.
Article in English | MEDLINE | ID: mdl-30349041

ABSTRACT

Macromolecular complexes are intrinsically flexible and often challenging to purify for structure determination by single-particle cryo-electron microscopy (cryo-EM). Such complexes can be studied by cryo-electron tomography (cryo-ET) combined with subtomogram alignment and classification, which in exceptional cases achieves subnanometer resolution, yielding insight into structure-function relationships. However, it remains challenging to apply this approach to specimens that exhibit conformational or compositional heterogeneity or are present in low abundance. To address this, we developed emClarity ( https://github.com/bHimes/emClarity/wiki ), a GPU-accelerated image-processing package featuring an iterative tomographic tilt-series refinement algorithm that uses subtomograms as fiducial markers and a 3D-sampling-function-compensated, multi-scale principal component analysis classification method. We demonstrate that our approach offers substantial improvement in the resolution of maps and in the separation of different functional states of macromolecular complexes compared with current state-of-the-art software.


Subject(s)
Cryoelectron Microscopy/methods , Electron Microscope Tomography/methods , Image Processing, Computer-Assisted/methods , Ribosome Subunits/ultrastructure , Software , gag Gene Products, Human Immunodeficiency Virus/ultrastructure , Humans , Saccharomyces cerevisiae
9.
Nat Commun ; 8(1): 629, 2017 09 20.
Article in English | MEDLINE | ID: mdl-28931821

ABSTRACT

The orientation distribution of a single-particle electron cryomicroscopy specimen limits the resolution of the reconstructed density map. Here we define a statistical quantity, the efficiency, E od, which characterises the orientation distribution via its corresponding point spread function. The efficiency measures the ability of the distribution to provide uniform information and resolution in all directions of the reconstruction, independent of other factors. This metric allows rapid and rigorous evaluation of specimen preparation methods, assisting structure determination to high resolution with minimal data.A number of parameters influence the resolution of a cryo-EM structure. Here the authors investigate the effects of specimen orientation in single particle cryo-EM and present open-source software for rapidly assessing orientation distributions to improve data collection.


Subject(s)
Cryoelectron Microscopy/methods , Imaging, Three-Dimensional/methods , Ribosome Subunits/ultrastructure , Algorithms , Efficiency , Fourier Analysis , Orientation, Spatial , Software , Specimen Handling
10.
Nature ; 546(7656): 113-117, 2017 06 01.
Article in English | MEDLINE | ID: mdl-28538735

ABSTRACT

Gene translation depends on accurate decoding of mRNA, the structural mechanism of which remains poorly understood. Ribosomes decode mRNA codons by selecting cognate aminoacyl-tRNAs delivered by elongation factor Tu (EF-Tu). Here we present high-resolution structural ensembles of ribosomes with cognate or near-cognate aminoacyl-tRNAs delivered by EF-Tu. Both cognate and near-cognate tRNA anticodons explore the aminoacyl-tRNA-binding site (A site) of an open 30S subunit, while inactive EF-Tu is separated from the 50S subunit. A transient conformation of decoding-centre nucleotide G530 stabilizes the cognate codon-anticodon helix, initiating step-wise 'latching' of the decoding centre. The resulting closure of the 30S subunit docks EF-Tu at the sarcin-ricin loop of the 50S subunit, activating EF-Tu for GTP hydrolysis and enabling accommodation of the aminoacyl-tRNA. By contrast, near-cognate complexes fail to induce the G530 latch, thus favouring open 30S pre-accommodation intermediates with inactive EF-Tu. This work reveals long-sought structural differences between the pre-accommodation of cognate and near-cognate tRNAs that elucidate the mechanism of accurate decoding.


Subject(s)
Cryoelectron Microscopy , Protein Biosynthesis , Ribosomes/metabolism , Ribosomes/ultrastructure , Anticodon/chemistry , Anticodon/genetics , Anticodon/ultrastructure , Codon/chemistry , Codon/genetics , Codon/ultrastructure , Escherichia coli/chemistry , Escherichia coli/genetics , Escherichia coli/ultrastructure , GTP Phosphohydrolases/metabolism , GTP Phosphohydrolases/ultrastructure , Guanosine Triphosphate/metabolism , Hydrolysis , Models, Molecular , Peptide Elongation Factor Tu/metabolism , Peptide Elongation Factor Tu/ultrastructure , Protein Domains , RNA, Ribosomal, 16S/genetics , RNA, Ribosomal, 16S/metabolism , RNA, Ribosomal, 16S/ultrastructure , RNA, Transfer, Amino Acyl/genetics , RNA, Transfer, Amino Acyl/metabolism , RNA, Transfer, Amino Acyl/ultrastructure , Ribosome Subunits/chemistry , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure , Ribosomes/chemistry
11.
Nucleic Acids Res ; 45(3): 1027-1040, 2017 02 17.
Article in English | MEDLINE | ID: mdl-28180306

ABSTRACT

Cryo-electron microscopy (cryo-EM) had played a central role in the study of ribosome structure and the process of translation in bacteria since the development of this technique in the mid 1980s. Until recently cryo-EM structures were limited to ∼10 Šin the best cases. However, the recent advent of direct electron detectors has greatly improved the resolution of cryo-EM structures to the point where atomic resolution is now achievable. This improved resolution will allow cryo-EM to make groundbreaking contributions in essential aspects of ribosome biology, including the assembly process. In this review, we summarize important insights that cryo-EM, in combination with chemical and genetic approaches, has already brought to our current understanding of the ribosomal assembly process in bacteria using previous detector technology. More importantly, we discuss how the higher resolution structures now attainable with direct electron detectors can be leveraged to propose precise testable models regarding this process. These structures will provide an effective platform to develop new antibiotics that target this fundamental cellular process.


Subject(s)
Bacteria/ultrastructure , Cryoelectron Microscopy/methods , Ribosomes/ultrastructure , Bacteria/chemistry , Bacteria/genetics , Models, Molecular , Protein Multimerization , Ribosomal Proteins/chemistry , Ribosome Subunits/chemistry , Ribosome Subunits/genetics , Ribosome Subunits/ultrastructure , Ribosomes/chemistry , Ribosomes/genetics
12.
Lett Appl Microbiol ; 64(1): 79-85, 2017 Jan.
Article in English | MEDLINE | ID: mdl-27739094

ABSTRACT

The ability of the ribosome to assist in folding of proteins both in vitro and in vivo is well documented and is a nontranslational function of the ribosome. The interaction of the unfolded protein with the peptidyl transferase centre (PTC) of the bacterial large ribosomal subunit is followed by release of the protein in the folding competent state and rapid dissociation of ribosomal subunits. Our study demonstrates that the PTC-specific antibiotics, chloramphenicol and blasticidin S inhibit unfolded protein-mediated subunit dissociation. During post-termination stage of translation in bacteria, ribosome recycling factor (RRF) is used together with elongation factor G to recycle the 30S and 50S ribosomal subunits for the next round of translation. Ribosome dissociation mediated by RRF and induced at low magnesium concentration was also inhibited by the antibiotics indicating that the PTC antibiotics exert an associative effect on ribosomal subunits. In vivo, the antibiotics can also reduce the ribosomal degradation during carbon starvation, a process requiring ribosome subunit dissociation. This study reveals a new mode of action of the broad-spectrum antibiotics chloramphenicol and blasticidin. SIGNIFICANCE AND IMPACT OF THE STUDY: Ribosome synthesizes protein in all organisms and is the target for multiple antimicrobial agents. Our study demonstrates that chloramphenicol and blasticidin S that target the peptidyl transferase centre of the bacterial ribosome can then inhibit dissociation of 70S ribosome mediated by (i) unfolded protein, (ii) translation factors or (iii) low Mg+2 concentrations in vitro and thereby suppresses ribosomal degradation during carbon starvation in vivo. The demonstration of this new mode of action furthers the understanding of these broad-spectrum antibiotics that differentially inhibit protein synthesis in prokaryotic and eukaryotic cells.


Subject(s)
Anti-Bacterial Agents/pharmacology , Chloramphenicol/pharmacology , Escherichia coli/drug effects , Protein Synthesis Inhibitors/pharmacology , Ribosomal Proteins/antagonists & inhibitors , Ribosome Subunits/metabolism , Crystallography, X-Ray , Nucleosides/pharmacology , Peptide Elongation Factor G , Peptidyl Transferases/antagonists & inhibitors , Prokaryotic Initiation Factor-3 , Protein Binding , Ribosome Subunits/drug effects , Ribosome Subunits/ultrastructure
13.
Annu Rev Biochem ; 85: 103-32, 2016 Jun 02.
Article in English | MEDLINE | ID: mdl-27023846

ABSTRACT

Mitochondrial ribosomes (mitoribosomes) perform protein synthesis inside mitochondria, the organelles responsible for energy conversion and adenosine triphosphate production in eukaryotic cells. Throughout evolution, mitoribosomes have become functionally specialized for synthesizing mitochondrial membrane proteins, and this has been accompanied by large changes to their structure and composition. We review recent high-resolution structural data that have provided unprecedented insight into the structure and function of mitoribosomes in mammals and fungi.


Subject(s)
DNA, Mitochondrial/genetics , Mitochondria/genetics , Mitochondrial Proteins/genetics , Mitochondrial Ribosomes/ultrastructure , Protein Biosynthesis , Ribosome Subunits/ultrastructure , Animals , Anti-Bacterial Agents/pharmacology , Biological Evolution , Carboxylic Ester Hydrolases/genetics , Carboxylic Ester Hydrolases/metabolism , DNA, Mitochondrial/metabolism , Mammals , Mitochondria/metabolism , Mitochondrial Membranes/metabolism , Mitochondrial Proteins/metabolism , Mitochondrial Ribosomes/chemistry , Mitochondrial Ribosomes/metabolism , Models, Molecular , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Transfer/genetics , RNA, Transfer/metabolism , Ribosome Subunits/chemistry , Ribosome Subunits/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism
14.
Structure ; 23(6): 977-8, 2015 Jun 02.
Article in English | MEDLINE | ID: mdl-26039346

ABSTRACT

In this issue of Structure, Chen et al. (2015) report the use of a mixing-spraying method of time-resolved cryogenic electron microscopy, which allowed the progression of ribosomal subunit association to be visualized on the millisecond timescale.


Subject(s)
Cryoelectron Microscopy/methods , Escherichia coli/chemistry , Models, Molecular , Ribosome Subunits/chemistry , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure
15.
Structure ; 23(6): 1097-105, 2015 Jun 02.
Article in English | MEDLINE | ID: mdl-26004440

ABSTRACT

Ribosomal subunit association is a key checkpoint in translation initiation but its structural dynamics are poorly understood. Here, we used a recently developed mixing-spraying, time-resolved, cryogenic electron microscopy (cryo-EM) method to study ribosomal subunit association in the sub-second time range. We have improved this method and increased the cryo-EM data yield by tenfold. Pre-equilibrium states of the association reaction were captured by reacting the mixture of ribosomal subunits for 60 ms and 140 ms. We also identified three distinct ribosome conformations in the associated ribosomes. The observed proportions of these conformations are the same in these two time points, suggesting that ribosomes equilibrate among the three conformations within less than 60 ms upon formation. Our results demonstrate that the mixing-spraying method can capture multiple states of macromolecules during a sub-second reaction. Other fast processes, such as translation initiation, decoding, and ribosome recycling, are amenable to study with this method.


Subject(s)
Cryoelectron Microscopy/methods , Escherichia coli/chemistry , Models, Molecular , Ribosome Subunits/chemistry , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure , Protein Conformation , Time Factors
16.
Nature ; 520(7549): 640-5, 2015 Apr 30.
Article in English | MEDLINE | ID: mdl-25901680

ABSTRACT

Ribosomes are translational machineries that catalyse protein synthesis. Ribosome structures from various species are known at the atomic level, but obtaining the structure of the human ribosome has remained a challenge; efforts to address this would be highly relevant with regard to human diseases. Here we report the near-atomic structure of the human ribosome derived from high-resolution single-particle cryo-electron microscopy and atomic model building. The structure has an average resolution of 3.6 Å, reaching 2.9 Å resolution in the most stable regions. It provides unprecedented insights into ribosomal RNA entities and amino acid side chains, notably of the transfer RNA binding sites and specific molecular interactions with the exit site tRNA. It reveals atomic details of the subunit interface, which is seen to remodel strongly upon rotational movements of the ribosomal subunits. Furthermore, the structure paves the way for analysing antibiotic side effects and diseases associated with deregulated protein synthesis.


Subject(s)
Cryoelectron Microscopy , Ribosomes/chemistry , Ribosomes/ultrastructure , Binding Sites , Electrons , Humans , Models, Molecular , RNA, Ribosomal/chemistry , RNA, Ribosomal/metabolism , RNA, Ribosomal/ultrastructure , RNA, Transfer/chemistry , RNA, Transfer/metabolism , RNA, Transfer/ultrastructure , Ribosomal Proteins/chemistry , Ribosomal Proteins/metabolism , Ribosomal Proteins/ultrastructure , Ribosome Subunits/chemistry , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure , Ribosomes/metabolism
17.
Science ; 346(6210): 718-722, 2014 Nov 07.
Article in English | MEDLINE | ID: mdl-25278503

ABSTRACT

Human mitochondrial ribosomes are highly divergent from all other known ribosomes and are specialized to exclusively translate membrane proteins. They are linked with hereditary mitochondrial diseases and are often the unintended targets of various clinically useful antibiotics. Using single-particle cryogenic electron microscopy, we have determined the structure of its large subunit to 3.4 angstrom resolution, revealing 48 proteins, 21 of which are specific to mitochondria. The structure unveils an adaptation of the exit tunnel for hydrophobic nascent peptides, extensive remodeling of the central protuberance, including recruitment of mitochondrial valine transfer RNA (tRNA(Val)) to play an integral structural role, and changes in the tRNA binding sites related to the unusual characteristics of mitochondrial tRNAs.


Subject(s)
Mitochondria/metabolism , RNA, Transfer, Val/chemistry , Ribosome Subunits/chemistry , Ribosome Subunits/ultrastructure , Binding Sites , Cryoelectron Microscopy , Humans , Mitochondria/genetics , Mitochondrial Proteins/chemistry , Mitochondrial Proteins/ultrastructure , Mutation , Nucleic Acid Conformation , Protein Conformation , RNA, Transfer, Val/analysis , Ribosome Subunits/genetics
18.
Nature ; 505(7484): 515-9, 2014 Jan 23.
Article in English | MEDLINE | ID: mdl-24362565

ABSTRACT

Mitochondrial ribosomes synthesize a number of highly hydrophobic proteins encoded on the genome of mitochondria, the organelles in eukaryotic cells that are responsible for energy conversion by oxidative phosphorylation. The ribosomes in mammalian mitochondria have undergone massive structural changes throughout their evolution, including ribosomal RNA shortening and acquisition of mitochondria-specific ribosomal proteins. Here we present the three-dimensional structure of the 39S large subunit of the porcine mitochondrial ribosome determined by cryo-electron microscopy at 4.9 Å resolution. The structure, combined with data from chemical crosslinking and mass spectrometry experiments, reveals the unique features of the 39S subunit at near-atomic resolution and provides detailed insight into the architecture of the polypeptide exit site. This region of the mitochondrial ribosome has been considerably remodelled compared to its bacterial counterpart, providing a specialized platform for the synthesis and membrane insertion of the highly hydrophobic protein components of the respiratory chain.


Subject(s)
Mitochondria/chemistry , Ribosome Subunits/chemistry , Animals , Cattle , Cryoelectron Microscopy , Hydrophobic and Hydrophilic Interactions , Mass Spectrometry , Mitochondria/ultrastructure , Mitochondrial Proteins/chemistry , Mitochondrial Proteins/ultrastructure , Models, Molecular , Nucleic Acid Conformation , Protein Conformation , RNA, Ribosomal, 16S/chemistry , RNA, Ribosomal, 16S/ultrastructure , Ribosomal Proteins/chemistry , Ribosomal Proteins/ultrastructure , Ribosome Subunits/ultrastructure , Swine
19.
Nature ; 497(7447): 80-5, 2013 May 02.
Article in English | MEDLINE | ID: mdl-23636399

ABSTRACT

Protein synthesis in all cells is carried out by macromolecular machines called ribosomes. Although the structures of prokaryotic, yeast and protist ribosomes have been determined, the more complex molecular architecture of metazoan 80S ribosomes has so far remained elusive. Here we present structures of Drosophila melanogaster and Homo sapiens 80S ribosomes in complex with the translation factor eEF2, E-site transfer RNA and Stm1-like proteins, based on high-resolution cryo-electron-microscopy density maps. These structures not only illustrate the co-evolution of metazoan-specific ribosomal RNA with ribosomal proteins but also reveal the presence of two additional structural layers in metazoan ribosomes, a well-ordered inner layer covered by a flexible RNA outer layer. The human and Drosophila ribosome structures will provide the basis for more detailed structural, biochemical and genetic experiments.


Subject(s)
Drosophila melanogaster/chemistry , Ribosomes/chemistry , Ribosomes/ultrastructure , Animals , Cryoelectron Microscopy , DNA-Binding Proteins/chemistry , Drosophila melanogaster/ultrastructure , Eukaryotic Cells , Evolution, Molecular , Humans , Models, Molecular , Molecular Conformation , Molecular Weight , Peptide Elongation Factor 2/metabolism , RNA, Ribosomal/chemistry , RNA, Ribosomal/metabolism , RNA, Ribosomal/ultrastructure , RNA, Transfer/chemistry , RNA, Transfer/metabolism , RNA, Transfer/ultrastructure , Ribosomal Proteins/chemistry , Ribosomal Proteins/isolation & purification , Ribosomal Proteins/metabolism , Ribosomal Proteins/ultrastructure , Ribosome Subunits/chemistry , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure , Ribosomes/metabolism , Saccharomyces cerevisiae Proteins/chemistry
20.
Nature ; 485(7399): 526-9, 2012 May 06.
Article in English | MEDLINE | ID: mdl-22622583

ABSTRACT

Bacterial ribosomes stalled at the 3' end of malfunctioning messenger RNAs can be rescued by transfer-messenger RNA (tmRNA)-mediated trans-translation. The SmpB protein forms a complex with the tmRNA, and the transfer-RNA-like domain (TLD) of the tmRNA then enters the A site of the ribosome. Subsequently, the TLD-SmpB module is translocated to the P site, a process that is facilitated by the elongation factor EF-G, and translation is switched to the mRNA-like domain (MLD) of the tmRNA. Accurate loading of the MLD into the mRNA path is an unusual initiation mechanism. Despite various snapshots of different ribosome-tmRNA complexes at low to intermediate resolution, it is unclear how the large, highly structured tmRNA is translocated and how the MLD is loaded. Here we present a cryo-electron microscopy reconstruction of a fusidic-acid-stalled ribosomal 70S-tmRNA-SmpB-EF-G complex (carrying both of the large ligands, that is, EF-G and tmRNA) at 8.3 Å resolution. This post-translocational intermediate (TI(POST)) presents the TLD-SmpB module in an intrasubunit ap/P hybrid site and a tRNA(fMet) in an intrasubunit pe/E hybrid site. Conformational changes in the ribosome and tmRNA occur in the intersubunit space and on the solvent side. The key underlying event is a unique extra-large swivel movement of the 30S head, which is crucial for both tmRNA-SmpB translocation and MLD loading, thereby coupling translocation to MLD loading. This mechanism exemplifies the versatile, dynamic nature of the ribosome, and it shows that the conformational modes of the ribosome that normally drive canonical translation can also be used in a modified form to facilitate more complex tasks in specialized non-canonical pathways.


Subject(s)
Escherichia coli/chemistry , Peptide Elongation Factor G/metabolism , Protein Biosynthesis , RNA, Bacterial/chemistry , RNA, Bacterial/metabolism , RNA-Binding Proteins/metabolism , Ribosomes/metabolism , Base Sequence , Cryoelectron Microscopy , Fusidic Acid/metabolism , Ligands , Models, Molecular , Nucleic Acid Conformation , Peptide Elongation Factor G/chemistry , Peptide Elongation Factor G/ultrastructure , Protein Binding , Protein Conformation , RNA, Bacterial/genetics , RNA, Bacterial/ultrastructure , RNA-Binding Proteins/chemistry , RNA-Binding Proteins/ultrastructure , Ribosome Subunits/chemistry , Ribosome Subunits/genetics , Ribosome Subunits/metabolism , Ribosome Subunits/ultrastructure , Ribosomes/chemistry , Ribosomes/genetics , Ribosomes/ultrastructure
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