ABSTRACT
Pulp tissue from three experimental dogs was tested for its antigenicity before and after incubation with Kerr (Rickert's) sealer (KS). Reactions to Arthus skin tests showed an increased response to pulp that had been incubated in KS, washed, and then injected, as compared with the material alone. Hemagglutinating antibody titers (1/360--1/600) were obtained from pulp tissue altered by KS. Inhibition tests showed the specificity of the antibody to be at a dilution of titer of 1:300. Therefore, a mixture of Kerr (Rickert's) sealer altered dog pulp tissue and rendered it antigenically active, and a specific humoral response was produced.
Subject(s)
Antibody Formation , Dental Pulp/immunology , Root Canal Filling Materials/immunology , Animals , Antibodies/analysis , Antigens , Arthus Reaction/immunology , Dental Pulp/drug effects , Dental Pulp Cavity/immunology , Dogs , Immunization , Male , Root Canal Filling Materials/pharmacology , Skin TestsABSTRACT
1. N2 paste when incubated with dog pulp tissue renders it antigenically active. 2. Specific systemic circulating antibody to dog pulp tissue altered by N2 paste was obtained. 3. When dog pulp tissue was altered by N2 paste a specific lymphocyte stimulation resulted. 4. The root canal is an effective mode of immunization.
