Antagonism of the ruthenium red-induced paralysis in mice by 4-aminopyridine, guanidine and lanthanum.

4-Aminopyridine and guanidine were administered intraperitoneally to mice during the complete flaccid paralysis induced by treatment with ruthenium red (RuR). At 1-8 min after 4-aminopyridine or guanidine the animals had recovered completely from the paralysis, whereas the control mice injected only with RuR remained paralytic for at least 60 min. Intraperitoneal injections of LaCl3 had no apparent effects on animal motility and did not reverse the paralysis produced by RuR. However, when La3 + was administered 30 min prior to RuR the occurrence of flaccid paralysis was totally prevented. The results obtained are discussed in terms of the possible antagonist effects of the compounds used on acetylcholine release at neuromuscular junctions.