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1.
Eur J Nutr ; 59(2): 685-697, 2020 Mar.
Article in English | MEDLINE | ID: mdl-30820652

ABSTRACT

PURPOSE: Dietary biomarkers allow the accurate and objective determination of the dietary intake of humans and can thus be valuable for investigating the relation between consumption of foods and biochemical as well as physiological responses. The objective of this study was the identification of potential urinary biomarkers for consumption of tomato juice. METHODS: In the course of a dietary intervention study, the human urine metabolome of a study cohort was compared between a tomato-free diet and after intake of tomato juice by application of an LC-HRMS-based metabolomics approach. The data acquisition was achieved using an orbitrap mass spectrometer, followed by multistage data processing and univariate as well as multivariate statistical analysis to identify discriminating features. RESULTS: Statistical analysis revealed several unique features detectable after tomato juice intake. The most discriminating markers were putatively identified as hydroxylated and sulfonated metabolites of esculeogenin B, aglycone of the steroidal glycoalkaloid esculeoside B recently found in tomato juice. Furthermore, the ß-carboline alkaloids tangutorid E and F and glucuronidated derivatives thereof were identified in urine. CONCLUSIONS: Steroidal glycoalkaloids in tomato juice are cleaved after ingestion, and hydroxylated and sulfonated metabolites of their aglycones might serve as urinary biomarkers for tomato juice intake. Similarly, ß-carboline alkaloids and glucuronidated derivatives were identified as potential urinary biomarkers. Both the aglycones of the steroidal alkaloids and the ß-carboline alkaloids might exhibit biological activities worth investigating.


Subject(s)
Diet/methods , Fruit and Vegetable Juices/statistics & numerical data , Mass Spectrometry/methods , Metabolomics/methods , Solanum lycopersicum , Adult , Biomarkers/urine , Carbolines/urine , Diet/statistics & numerical data , Female , Humans , Male , Sapogenins/urine , Young Adult
2.
J Pharm Biomed Anal ; 88: 497-508, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24184656

ABSTRACT

20(S)-Protopanaxatriol (PPT), one of the aglycones of ginsenosides, has been shown to exert cardioprotective effects against myocardial ischemic injury. However, studies on PPT metabolism have rarely been reported. This study is the first to investigate the in vivo metabolism of PPT following oral administration by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-Q/TOF-MS) and nuclear magnetic resonance (NMR) spectroscopy. The structures of the metabolites were identified based on the characteristics of their MS data, MS(2) data, and chromatographic retention times. A total of 22 metabolites, including 17 phase I and 5 phase II metabolites, were found and tentatively identified by comparing their mass spectrometry profiles with those of PPT. Two new monooxygenation metabolites, (20S,24S)-epoxy-dammarane-3,6,12,25-tetraol and (20S,24R)-epoxy-dammarane-3,6,12,25-tetraol, were chemicallly synthesized and unambiguously characterized according to the NMR spectroscopic data. The metabolic pathways of PPT were proposed accordingly for the first time. Results revealed that oxidation of (1) double bonds at Δ((24,25)) to form 24,25-epoxides, followed by rearrangement to yield 20,24-oxide forms; and (2) vinyl-methyl at C-26/27 to form corresponding carboxylic acid were the predominant metabolic pathways. Phase II metabolic pathways were proven for the first time to consist of glucuronidation and cysteine conjugation. This study provides valuable and new information on the metabolism of PPT, which is indispensable for understanding the safety and efficacy of PPT, as well as its corresponding ginsenosides.


Subject(s)
Sapogenins/urine , Administration, Oral , Animals , Chromatography, High Pressure Liquid , Chromatography, Ion Exchange , Cysteine/chemistry , Epoxy Compounds/chemistry , Feces , Ginsenosides/urine , Magnetic Resonance Spectroscopy , Male , Molecular Structure , Oxygen/chemistry , Rats , Rats, Sprague-Dawley , Sapogenins/pharmacokinetics , Software , Spectrometry, Mass, Electrospray Ionization , Tandem Mass Spectrometry
3.
Article in English | MEDLINE | ID: mdl-23877070

ABSTRACT

In this study, ultra-performance liquid chromatography (UPLC)/quadrupole-time-of-flight mass spectrometry (QTOF-MS) was applied to the rapid analysis of 20(S)-protopanaxadiol (PPD) metabolites in rats after oral administration, enabling the structural characterization of 23 metabolites in plasma, bile, urine, and feces. 16 of these, including M1-M5, M9, and M11-M15, have not been previously reported. The results also indicated that demethylation, dehydration, dehydrogenation, oxidation, deoxidation, and glucuronidation were the major metabolic reactions of PPD in vivo. This study provides important information about the metabolism of PPD which will be helpful for fully understanding its mechanism of action. Furthermore, structural modification of PPD in vivo may aid in obtaining new chemical derivatives for pharmacological screening.


Subject(s)
Chromatography, High Pressure Liquid/methods , Drugs, Chinese Herbal/analysis , Drugs, Chinese Herbal/metabolism , Mass Spectrometry/methods , Panax/chemistry , Sapogenins/analysis , Sapogenins/metabolism , Animals , Bile/chemistry , Bile/metabolism , Drugs, Chinese Herbal/administration & dosage , Feces/chemistry , Male , Rats , Rats, Sprague-Dawley , Sapogenins/blood , Sapogenins/urine
4.
J Chromatogr B Analyt Technol Biomed Life Sci ; 879(22): 2011-7, 2011 Jul 15.
Article in English | MEDLINE | ID: mdl-21704572

ABSTRACT

Ginsenoside Re (G-Re) improved the memory function of experimental animals in a preclinical study. Several types of saponins including G-Rg1, G-Rg2, G-F1, G-Rh1, and protopanaxatriol (PPT) may be the metabolites of G-Re according to reports from preclinical trials. In order to support a study of the pharmacokinetics of G-Re, an analytical method for G-Re and the co-detection of its probable metabolites using liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed and validated. Solid phase extraction was utilized in the sample preparation. Separation of the analytes was achieved using a gradient elution (0.05% formic acid-methanol-acetonitrile, each organic phase containing 0.05% formic acid) at a flow rate of 0.3 mL/min with a retention time of approximately 2.88 min for G-Re. Data were acquired in the multiple reaction mode (MRM) and the linear range of the standard curve of plasma and urine samples for G-Re was 0.05-20 ng/mL with r(2)≥0.99. In the analysis of probable metabolites, G-Re, G-Rg1, G-F1, G-Rh1 and PPT were all detected in samples; however, G-Rg2 was not detected.


Subject(s)
Chromatography, Liquid/methods , Ginsenosides/blood , Ginsenosides/urine , Sapogenins/blood , Sapogenins/urine , Tandem Mass Spectrometry/methods , Drug Stability , Ginsenosides/metabolism , Ginsenosides/pharmacokinetics , Humans , Linear Models , Reproducibility of Results , Sapogenins/metabolism , Sapogenins/pharmacokinetics , Sensitivity and Specificity , Solid Phase Extraction
5.
Drug Metab Dispos ; 31(8): 1065-71, 2003 Aug.
Article in English | MEDLINE | ID: mdl-12867496

ABSTRACT

Even though the degradation of ginsenosides has been thoroughly studied in animals and in vitro using acids, enzymes, and intestinal bacteria, knowledge concerning the systemic availability of ginsenosides and their degradation products in humans is generally lacking. Therefore, the attention in this article is focused on the identification of ginsenosides and their hydrolysis products reaching the systemic circulation in man. This is of great importance in understanding clinical effects, preventing herb-drug interactions, and optimizing the biopharmaceutical properties of ginseng preparations. Using a sensitive mass spectrometric method, which is specific for the identification of ginsenosides in complex biological matrices, the degradation pathway of ginsenosides in the gastrointestinal tract of humans could be elucidated following the oral administration of ginseng. Within the frame of a pilot study, human plasma and urine samples of two subjects were screened for ginsenosides and their possible degradation products. In general, the urine data coincided well with the plasma data. In both volunteers the same hydrolysis products, which are not originally present in the Ginsana extract (Pharmaton S.A., Lugano, Switzerland) ingested, were identified in plasma and urine. It was shown that two hydrolysis products of the protopanaxatriol ginsenosides, namely G-Rh1 and G-F1 may reach the systemic circulation. In addition, compound-K, the main intestinal bacterial metabolite of the protopanaxadiol ginsenosides, was detected in plasma and urine. These products are probably responsible for the action of ginseng in humans. In opposition to previous reports, G-Rb1 was identified in plasma and urine of one subject.


Subject(s)
Ginsenosides/administration & dosage , Ginsenosides/metabolism , Administration, Oral , Biotransformation , Capsules , Ginsenosides/blood , Ginsenosides/chemistry , Ginsenosides/pharmacokinetics , Ginsenosides/urine , Humans , Mass Spectrometry , Sapogenins/blood , Sapogenins/chemistry , Sapogenins/urine , Triterpenes/blood , Triterpenes/chemistry , Triterpenes/urine
6.
J Chromatogr B Biomed Sci Appl ; 689(2): 349-55, 1997 Feb 21.
Article in English | MEDLINE | ID: mdl-9080321

ABSTRACT

An improved gas chromatographic-mass spectrometric method (GC-MS) with a fast solid-phase extraction on a newly introduced C18 microcolumn, was applied to study the urinary excretion 20(S)-protopanaxadiol and 20(S)-protopanaxatriol glycosides in man after oral administration of ginseng preparations. Using panaxatriol as internal standard, 20(S)-protopanaxadiol and 20(S)-protopanaxatriol (the aglycones of ginsenosides) could be determined at a detection level of a few ng per ml urine by GC-MS with selected-ion monitoring after their release from glycosides which occur in urine. The extraction recovery of ginsenosides from urine was more than 80% and the intra-assay coefficient of variation was less than 5.0%. The results after intake of single doses of ginseng preparations demonstrated a linear relation between the amounts of ginsenosides consumed and the 20(S)-protopanaxatriol glycosides excreted in urine. About 1.2% of the dose was recovered in five days.


Subject(s)
Gas Chromatography-Mass Spectrometry/methods , Panax/metabolism , Plants, Medicinal , Sapogenins/urine , Saponins/metabolism , Triterpenes/urine , Ginsenosides , Humans
7.
Scand J Clin Lab Invest ; 56(2): 151-60, 1996 Apr.
Article in English | MEDLINE | ID: mdl-8743108

ABSTRACT

Recently developed gas chromatographic and gas chromatographic-mass spectrometric methods were used to characterize 17 different commercial ginseng preparations sold in Sweden. The contents of total ginsenosides per capsule or per tablet varied from 2.1 to 13.3 mg. Unlike the other preparations, a red ginseng and three liquid ginseng preparations (after releasing the sugar moieties from ginsenosides) were shown also to contain significant amounts of 20-epimers of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol as well as their corresponding 24,25-hydrated compounds. In addition to the genuine and artificial sapogenins mentioned above, two epimeric pairs of prosapogenines (ginsenoside Rg3 and 20(S)-Rg3, ginsenoside Rh1 and 20(R)-Rh1) were also found in the liquid formulations. These results suggest that hydrolysis, epimerization and hydration in the side-chain of the aglycone moiety of ginsenosides may occur in the liquid formulations under weak acidic conditions (pH 3.0-3.5 with 9-10% of alcohol at room temperature). The new method was also used to determine the aglycones of ginsenosides in urine samples from Swedish athletes stating that they had consumed ginseng preparations within 10 days before urine collection. Out of the 65 samples analysed, 60 were found to contain 20(S)-protopanaxatriol. The concentrations of 20(S)-protopanaxatriol ginsenosides varied from 2 to 35 ng ml-1 urine. This is the first demonstration of uptake of ginsenosides in humans after oral administration of ginseng preparations.


Subject(s)
Glycosides/chemistry , Panax/chemistry , Plants, Medicinal , Sapogenins/urine , Saponins/chemistry , Sports , Triterpenes , Ginsenosides , Glycosides/urine , Humans , Sapogenins/chemistry , Saponins/urine , Sweden
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