Subject(s)
Epitopes/analysis , Leukemia Virus, Murine/immunology , Sarcoma Viruses, Murine/immunology , Viral Proteins/immunology , Electrophoresis, Polyacrylamide Gel , Gene Products, gag , Moloney murine leukemia virus/enzymology , Peptide Hydrolases/metabolism , Protein Precursors/metabolism , Sarcoma Viruses, Murine/ultrastructure , Viral Proteins/metabolismABSTRACT
HTG2 hamster cells produce a defective murine sarcoma virus lacking gp70 and, consequently, viral surface projections (knobs), but the lack of knobs appears to have no effect on intramembrane particle distribution. In addition, it has been noted that the core of the virus remains in the "immature" form as a result of the failure of the polyprotein precursor (p65) to undergo cleavage. However, incubation of HTG2 virus with avian myoblastosis virus was found to yield specific cleavage products of p65.
Subject(s)
Defective Viruses/ultrastructure , Sarcoma Viruses, Murine/ultrastructure , Viral Proteins/metabolism , Animals , Avian Myeloblastosis Virus/physiology , Cell Line , Cricetinae , Electrophoresis, Polyacrylamide Gel , Helper Viruses/physiology , Microscopy, Electron , Sarcoma Viruses, Murine/metabolism , Thymus GlandABSTRACT
This study describes an unusual type of virus seen when a feline leukemia virus (FeLV) pseudotype of murine sarcoma virus (MuSV) obtained by cocentrifugation procedures infected feline embryo cells (FEF) and two Crandell cat cell lines (CrFK1, CrFK2). When all three cell cultures were infected with MuSV-FeLV, only FEF and CrFK2 were transformed and only these showed normal and aberrant virus. The CrFK1 infected with MuSV-FeLV did not transform but did replicate normal type-C virus with a 50-A intermediate coat. The virus replicated in the two transformed lines showed three particles; a normal particle with a 50-A intermediate coat, a normal particle with a 100-A intermediate coat, and an aberrant particle with a 100-A intermediate coat.
