ABSTRACT
The scyphozoan jellyfish Cyanea capillata and Nemopilema nomurai are common blooming species in China. They possess heterogeneous nematocysts and produce various types of venom that can elicit diverse sting symptoms in humans. However, the differences in venom composition between the two species remain unclear. In this study, a combined transcriptomic and proteomic approach was used to identify and compare putative toxins in penetrant nematocysts isolated from C. capillata and N. nomurai. A total of 53 and 69 putative toxins were identified in C. capillata nematocyst venom (CnV) and N. nomurai nematocyst venom (NnV), respectively. These sting-related toxins from both CnV and NnV could be grouped into 10 functional categories, including proteinases, phospholipases, neurotoxins, cysteine-rich secretory proteins (CRISPs), lectins, pore-forming toxins (PFTs), protease inhibitors, ion channel inhibitors, insecticidal components, and other toxins, but the constituent ratio of each toxin category varied between CnV and NnV. Metalloproteinases, proteases, and pore-forming toxins were predominant in NnV, representing 27.5%, 18.8%, and 8.7% of the identified venom proteins, respectively, while phospholipases, neurotoxins, and proteases were the top three identified venom proteins in CnV, accounting for 22.6%, 17.0%, and 11.3%, respectively. Our findings provide comprehensive information on the molecular diversity of toxins from two common blooming and stinging species of jellyfish in China. Furthermore, the results reveal a possible relationship between venom composition and sting consequences, guiding the development of effective treatments for different jellyfish stings.
Subject(s)
Cnidaria/chemistry , Cnidarian Venoms/chemistry , Scyphozoa/chemistry , Toxins, Biological/chemistry , Animals , Bites and Stings , China , Cnidaria/genetics , Cnidaria/pathogenicity , Gene Expression Profiling , Proteomics , Scyphozoa/genetics , Scyphozoa/pathogenicityABSTRACT
Jellyfish are a type of poisonous cnidarian invertebrate that secrete lethal venom for predation or defense. Human beings often become victims of jellyfish stings accidentally while swimming or fishing and suffer severe pain, itching, swelling, inflammation, shock, and even death. Jellyfish venom is composed of various toxins, and the lethal toxin is the most toxic and hazardous component of the venom, which is responsible for deaths caused by jellyfish stings and envenomation. Our previous study revealed many toxins in jellyfish venom, including phospholipase A2, metalloproteinase, and protease inhibitors. However, it is still unknown which type of toxin is lethal and how it works. Herein a combined toxicology analysis, proteome strategy, and purification approach was employed to investigate the lethality of the venom of the jellyfish Cyanea nozakii. Toxicity analysis revealed that cardiotoxicity including acute myocardial infarction and a significant decrease in both heart rate and blood pressure is the primary cause of death. Purified lethal toxin containing a fraction of jellyfish venom was subsequently subjected to proteome analysis and bioinformation analysis. A total of 316 and 374 homologous proteins were identified, including phospholipase A2-like toxins and metalloprotease-like toxins. Furthermore, we confirmed that the lethality of the jellyfish venom is related to metalloproteinase activity but without any phospholipase A2 activity or hemolytic activity. Altogether, this study not only provides a comprehensive understanding of the lethal mechanism of jellyfish venom but also provides very useful information for the therapeutic or rescue strategy for severe jellyfish stings.
Subject(s)
Cnidarian Venoms/chemistry , Metalloproteases/isolation & purification , Myocardial Infarction/chemically induced , Phospholipases A2/isolation & purification , Proteome/isolation & purification , Scyphozoa/chemistry , Animals , Blood Pressure/drug effects , Brain/drug effects , Brain/physiopathology , Chromatography, Liquid , Cnidarian Venoms/toxicity , Female , Gene Ontology , Heart/drug effects , Heart/physiopathology , Heart Rate/drug effects , Kidney/drug effects , Kidney/physiopathology , Liver/drug effects , Liver/physiopathology , Lung/drug effects , Lung/physiopathology , Male , Mass Spectrometry , Metalloproteases/chemistry , Metalloproteases/toxicity , Mice , Molecular Sequence Annotation , Myocardial Infarction/physiopathology , Phospholipases A2/chemistry , Phospholipases A2/toxicity , Proteome/chemistry , Proteome/classification , Proteome/toxicity , Proteomics/methods , Scyphozoa/pathogenicity , Scyphozoa/physiology , Spleen/drug effects , Spleen/physiopathologyABSTRACT
Modified handling is often claimed to reduce (sub-)lethal impacts among organisms caught-and-released in fisheries. Improving welfare of discarded fish warrants investigation, when their survival is of both economic and ecological importance. In this study, juvenile yellowfin bream (Acanthopagrus australis) were trawled in an Australian penaeid fishery and then discarded after on-board sorting in either dry or water-filled (modified) trays and with delays in starting sorting of either 2 or 15 mins. Blood plasma cortisol, glucose and potassium were sampled immediately from some yellowfin bream, while others were placed into cages (with controls) and sampled after five days. Irrespective of their on-board handling, all trawled fish incurred a relatively high acute stress response (i.e. an increase in Mean ± SE cortisol from a baseline of <4 to 122.0 ± 14.9 ng/mL) that was mostly attributed to the trawling process, and exacerbated by variation in key parameters (low salinity, changes in water temperature and the presence of jellyfish Catostylus mosaicus in catches). When C. mosaicus was present, the potassium concentrations of fish sampled immediately after sorting were significantly elevated, possibly due to nematocyst contact and subsequent inhibition of ion pumps or cytolysis. Stress also increased during handling in response to warmer air temperatures and longer exposure. While most fish had substantially recovered by 120 hours after discarding, deploying selective trawls (to reduce jellyfish) for short periods and then quickly sorting catches in water would benefit discard welfare.
Subject(s)
Animal Welfare , Fisheries , Perciformes/physiology , Stress, Physiological , Animals , Australia , Blood Glucose/metabolism , Hydrocortisone/blood , Perciformes/blood , Perciformes/parasitology , Potassium/blood , Salinity , Scyphozoa/pathogenicity , Seawater/chemistry , TemperatureSubject(s)
Anaphylaxis/etiology , Food Hypersensitivity/etiology , Scyphozoa/immunology , Soy Foods/adverse effects , Anaphylaxis/immunology , Animals , Bites and Stings/immunology , Food Hypersensitivity/immunology , Humans , Male , Middle Aged , Polyglutamic Acid/analogs & derivatives , Polyglutamic Acid/immunology , Scyphozoa/pathogenicityABSTRACT
Jellyfish are efficient predators which prey on crabs, fish larvae, and small fish. Their venoms consist of various toxins including neurotoxins that paralyse prey organisms immediately. One possible mode of action of neurotoxins is the blockage of voltage-gated sodium (Na(v)) channels. A novel polypeptide with Na(v) channel blocking activity was isolated from the northern Scyphozoa Cyanea capillata (L., 1758). For that purpose, a bioactivity-guided multidimensional liquid chromatographic purification method has been developed. A neurotoxic activity of resulting chromatographic fractions was demonstrated by a bioassay, which based on the mouse neuroblastoma cell line Neuro2A. The purification process yielded one fraction containing a single polypeptide with proven activity. The molecular weight of 8.22 kDa was determined by matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-ToF MS). Utilising Laser Microdissection and Pressure Catapulting (LMPC) for the separation of different nematocyst types in combination with direct MALDI-ToF MS analysis of the intact capsules, the neurotoxin was found to be present in all types of fishing tentacle isorhizas (A-isorhizas, a-isorhizas, O-isorhizas) of C. capillata medusae.
Subject(s)
Neurotoxins/isolation & purification , Scyphozoa/pathogenicity , Sodium Channel Blockers/isolation & purification , Animals , Cell Line, Tumor , Mice , Molecular Weight , Neurotoxins/toxicity , Sodium Channel Blockers/toxicity , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
The present study, for the first time, comparatively investigated the enzymatic activities (proteases and hyaluronidases) in the venoms of four Scyphozoan jellyfish species, including Nemopilema nomurai, Rhopilema esculenta, Cyanea nozakii, and Aurelia aurita. For this, various zymographic analyses were performed using assay specific substrates. Interestingly, all the four jellyfish venoms showed gelatinolytic, caseinolytic, and fibrinolytic activities, each of which contains a multitude of enzyme components with molecular weights between 17 and 130 kDa. These four jellyfish venoms demonstrated a huge variation in their proteolytic activities in quantitative and qualitative manner depending on the species. Most of these enzymatic activities were disappeared by the treatment of 1,10-phenanthroline, suggesting they might be belonged to metalloproteinases. Toxicological significance of these venom proteases was examined by comparing their proteolytic activity and the cytotoxicity in NIH 3T3 cells. The relative cytotoxic potency was C. nozakii > N. nomurai > A. aurita > R. esculenta. The cytotoxicity of jellyfish venom shows a positive correlation with its overall proteolytic activity. The metalloproteinases appear to play an important role in the induction of jellyfish venom toxicities. In conclusion, the present report proposes a novel finding of Scyphozoan jellyfish venom metalloproteinases and their potential role in the cytotoxicity.
Subject(s)
Cnidarian Venoms/toxicity , Cytotoxins/toxicity , Metalloproteases/toxicity , Scyphozoa/pathogenicity , Animals , Cnidarian Venoms/enzymology , Hyaluronoglucosaminidase/metabolism , Metalloendopeptidases/metabolism , Mice , NIH 3T3 Cells , Scyphozoa/enzymologyABSTRACT
Swarms or blooms of jellyfish are increasingly problematic and can result in high mortality rates of farmed fish. Small species of jellyfish, such as Phialella quadrata (13 mm in diameter), are capable of passing through the mesh of sea cages and being sucked into the mouth of fish during respiration. Results of the current study show that the initial damage to gills of farmed Atlantic salmon, likely produced by nematocyst-derived toxins from the jellyfish, was compounded by secondary bacterial infection with Tenacibaculum maritimum. Results also demonstrate that these filamentous bacteria were present on the mouth of the jellyfish and that their DNA sequences were almost identical to those of bacteria present on the salmon gills. This suggests that the bacterial lesions were not the result of an opportunistic infection of damaged tissue, as previously thought. Instead, P. quadrata is probably acting as a vector for this particular bacterial pathogen, and it is the first time that evidence to support such a link has been presented. No prior literature describing the presence of bacteria associated with jellyfish, except studies about their decay, could be found. It is not known if all jellyfish of this and other species carry similar bacteria or the relationship to each other. Their source, the role they play under other circumstances, and indeed whether the jellyfish were themselves diseased are also not known. The high proteolytic capabilities of T. maritimum mean that partially digested gill tissues were readily available to the jellyfish, which rely heavily on intracellular digestion for their nutrition.
Subject(s)
Bacterial Infections/veterinary , Disease Vectors/classification , Fish Diseases/microbiology , Scyphozoa/microbiology , Animals , Bacterial Infections/epidemiology , Bacterial Infections/pathology , Bacterial Infections/transmission , Conserved Sequence , DNA Primers , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Fish Diseases/epidemiology , Fish Diseases/pathology , Gills/microbiology , Gills/pathology , Polymerase Chain Reaction , RNA, Bacterial/chemistry , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/chemistry , RNA, Ribosomal, 16S/genetics , Salmo salar , Scotland/epidemiology , Scyphozoa/genetics , Scyphozoa/pathogenicityABSTRACT
Las medusas son animales marinos invertebrados, de poca movilidadque son transportados por las corrientes marinas. Su composición es deun 95% de agua y por tanto, se camuflan fácilmente. El contacto conlos tentáculos es urticante. El cambio climático que está experimentandoel planeta en los últimos años ha supuesto un aumento de la temperaturadel agua del mar que ha provocado a su vez un aumento notablede la población de medusas en verano.Existen variedad de tratamientos populares para la picadura de medusa,como por ejemplo, aplicar vinagre, alcohol yodado o povidona yodada,alcohol, amoníaco, pomada de hidrocortisona o cremas antiinflamatorias.Nuestra experiencia de varios años, así como el contenido de publicacionesconsultadas, sugiere que el uso de povidona yodada al 10% es eficazcomo antiséptico en el tratamiento de las lesiones producidas porlas picaduras de medusa.El objetivo de este trabajo es dar a conocer la intervención enfermera quellevamos a cabo en nuestro centro ante la picadura de medusa y proponerel uso de povidona yodada al 10%, como antiséptico de elección (AU)
Jellyfish are invertebrate marine animals with little mobility that aretransported by ocean currents. Their composition is 95% water andthus they are easily camouflaged. Skin contact with their tentacles causesskin itching (urticary). The climate change that has been taking placeon the planet for the past few years has led to an increase in sea temperature,which in turn has triggered considerable growth of jellyfishpopulations during the summer season.There are many popular treatment options for jellyfish stings, such as applyingvinegar, alcohol-iodine or povidone-iodine, alcohol, ammonia, hydrocortisonepomade or anti-inflammatory creams.Our several years of experience, as well as the content of consulted publications,suggests the use of 10% povidone-iodine as an effective antisepticoption in the treatment of lesions caused by jellyfish stings.This work aims to describe the nursing intervention that is carried outin our centre when we are faced with jellyfish stings and to propose theuse of 10% povidone-iodine as the antiseptic solution of choice (AU)
Subject(s)
Humans , Cnidarian Venoms/adverse effects , Poisoning/nursing , Scyphozoa/pathogenicity , Urticaria/etiology , Hypersensitivity/nursingABSTRACT
Over the past few years, populations of the giant jellyfish Nemopilema nomurai (Scyphozoa: Rhizostomeae) have increased dramatically in the waters of China, Korea, and Japan without any definitive reason. This has resulted in severe damage to fisheries in the areas. During a pilot study, we observed that the venom of N. nomurai produced a functional cardiac depression in mice. However, the mechanism of action was not examined. In the present study, we investigated the cardiovascular effects of nematocyst-derived venom from N. nomurai in anesthetized rats. Venom (0.1-2.4 mg protein/kg, i.v.) produced dose-dependent hypotension (65+/-12% of initial at a cumulative dose of 3 mg/kg) and bradycardia (80+/-5% of initial at a cumulative dose of 3 mg/kg). At the highest dose, this was characterized by a transient decrease in blood pressure (phase 1) followed by a return to basal level and then a slower decrease in blood pressure (phase 2). Venom also produced a decrease in rate and force of contraction in the rat isolated atria. Interestingly, venom induced a contraction of isolated aortic rings which was blocked by felodipine but not by prazosin, suggesting the contraction is mediated by calcium channel activation. These results suggest that the negative inotropic and chronotropic effects of the venom of N. nomurai may be due to a direct effect on the heart.
Subject(s)
Blood Pressure/drug effects , Cnidarian Venoms/toxicity , Heart Rate/drug effects , Scyphozoa/pathogenicity , Animals , Male , Myocardial Contraction/drug effects , Pulse , Rats , Rats, Sprague-Dawley , Vasoconstriction/drug effectsABSTRACT
Three edible jellyfish Rhopilema hispidum, R. esculentum and Nemopilema nomurai are virulent to humans. We monitored one patient that was stung sequentially by these three species of jellyfish. The first species caused a persistent eruption, the second produced significant pruritus and the last induced only cutaneous symptoms rather than severe systemic disorders reported for its Chinese counterpart. The lesions of these jellyfish species are characteristic and common in workers harvesting medusae. There is no significant incidence of symptoms by ingesting these animals.
Subject(s)
Bites and Stings/pathology , Scyphozoa/pathogenicity , Animals , Bites and Stings/diagnosis , Humans , Japan , Oceans and Seas , Scyphozoa/classification , SeafoodABSTRACT
Las lesiones por picaduras de medusa son un motivo de consulta frecuente en las zonas costeras en época de verano. Se relacionan con una toxina que inyectan las medusas al contacto con la piel. En general, suelen ocasionar cuadros de predominio dermatológico. Pero, en ocasiones, aparecen complicaciones por sobreinfección de las lesiones cutáneas, que deben ser controladas con tratamiento antibiótico y oclusión hasta que el tejido afectado se regenere y cicatrice. Presentamos el caso de una niña de 6 años de edad, que acudió al servicio de Urgencias por presentar dos lesiones en antebrazo izquierdo, una de ellas lineal y la otra puntiforme, de borde eritematoso y elevado (AU)
Subject(s)
Female , Child , Humans , Bites and Stings/drug therapy , Cnidarian Venoms/poisoning , Bites and Stings/diagnosis , Coasts , Forearm , Erythema/etiology , Fibrinolysin/pharmacology , Fibrinolysin/administration & dosage , Ointments/pharmacology , Scyphozoa/pathogenicityABSTRACT
A espécie Physalia sp é o tipo de caravela mais freqüente e de maior atividade tóxica do litoral brasileiro, causando acidentes por contatos, com sintomatologia de dor, eritema, prurido, contrações musculares, vômitos, podendo ocorrer também hemólise, insuficiência renal e choque anafilático. Analisou-se a frequencia destes acidentes, notificados ao centro de Informações Toxicológicas (CIT/SC) no período de 1984 a 1996....
Subject(s)
Humans , Male , Female , Adult , Epidemiology/trends , Scyphozoa/pathogenicity , Poisoning/microbiology , Scyphozoa/virologyABSTRACT
Existen muy escasas referencias bibliográficas de envenenamiento por Cnidaria (celenterados) de las costas del Oceáno Atlántico Medio. En este trabajo se describen tres casos producidos por el veneno del Hidrozoa Syphonophora Physalia physalis (L.) de la costa oceánica uruguaya
