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1.
J Vet Diagn Invest ; 32(4): 594-597, 2020 Jul.
Article in English | MEDLINE | ID: mdl-32495719

ABSTRACT

We assessed the causes of polyserositis in pigs, categorized by causative agents and ages of animals affected. In a 3-y study, 246 pigs from 80 different farms with recurrent problems of polyserositis, in a high-density breeding area, were submitted for autopsy; 154 pigs with typical fibrinous serosal lesions were sampled for further bacterial and viral investigation. The most common gross lesions were pleuritis and pericarditis (141 of 154; 92%). The animals most affected were weaned pigs (139 of 154; 90%). Haemophilus parasuis and Mycoplasma hyorhinis were the most common bacteria detected and were present at the same rate (85 of 154; 55%). Other bacteria isolated were Streptococcus sp. (44 of 154; 29%), Pasteurella multocida (21 of 154; 14%), Escherichia coli (19 of 154; 12%), Actinobacillus pleuropneumoniae (7 of 154; 5%), and Trueperella pyogenes (4 of 154; 3%). Porcine reproductive and respiratory syndrome virus (PRRSV; 119 of 154; 77%) predominated among the viruses detected, followed, with lesser prevalence, by porcine circovirus 2 (40 of 154; 26%) and swine influenza A virus (19 of 154; 12%). Bacterial coinfection and coinfection of bacteria and viruses were common (128 of 154; 83%). A strong positive correlation was found between coinfection by H. parasuis and M. hyorhinis and also by H. parasuis with PRRSV.


Subject(s)
Serositis/veterinary , Swine Diseases/epidemiology , Animals , Bacterial Infections/classification , Bacterial Infections/epidemiology , Bacterial Infections/microbiology , Bacterial Infections/veterinary , Italy/epidemiology , Serositis/epidemiology , Serositis/microbiology , Serositis/virology , Sus scrofa , Swine , Swine Diseases/microbiology , Swine Diseases/virology , Virus Diseases/classification , Virus Diseases/epidemiology , Virus Diseases/veterinary , Virus Diseases/virology
2.
Sci Rep ; 9(1): 1480, 2019 02 06.
Article in English | MEDLINE | ID: mdl-30728364

ABSTRACT

A definitive diagnosis of tuberculosis serositis (TS) is still challenging. Our preliminary practice found that Serous Fluid Drainage Flocky Precipitate (SFDFP) was a useful testing sample to diagnose TS. We designed this study to assess the diagnostic performance of SFDPF for TS compared with conventional bacteriology methods on serous fluid (SF). A cohort study was conducted from July 2014 to April 2016. Patients with suspected TS were consecutively screened. SF and SFDFP were collected and tested by Ziehl-Neelsen stain, MTB culture, and Xpert/RIF assay. We compared the diagnostic performance of SF and SFDFP in several test settings. Through this study, 85 patients were enrolled, of whom 70 (82.4%) were confirmed TS or highly probable TS, 13 (15.3%) were none-TS and 2 (2.4%) indeterminate results were ruled out. The overall sensitivity using both SFDFP and SF was significantly higher than each (60% vs. 48% and 41%, p < 0.05). SFDFP and SF samples had similar diagnostic performance (p < 0.05). No false positive was detected in this study. We concluded that SFDFP is a reliable testing sample for diagnosing tuberculous serositis. SFDFP may significantly improve the diagnostic yield as a supplement to conventional tests.


Subject(s)
Molecular Diagnostic Techniques/methods , Serositis/metabolism , Tuberculosis, Cardiovascular/diagnosis , Adolescent , Adult , Aged , Aged, 80 and over , Body Fluids/diagnostic imaging , Body Fluids/metabolism , Child , Child, Preschool , China , Cohort Studies , Female , Humans , Male , Middle Aged , Mycobacterium tuberculosis/genetics , Sensitivity and Specificity , Serositis/diagnosis , Serositis/microbiology , Specimen Handling/methods , Sputum/microbiology , Tuberculosis, Meningeal/diagnosis
3.
Biomed Mater Eng ; 26 Suppl 1: S2123-32, 2015.
Article in English | MEDLINE | ID: mdl-26405991

ABSTRACT

The Yinzhihuang injection, a traditional Chinese medicine, has been the recent target of increasing interest due to its anti-inflammatory properties. The molecular basis by which Yinzhihuang injection could cure Riemerella anatipestifer (RA) serositis in ducks is unclear. This study evaluated the antibacterial, anti-inflammatory and antioxidant effects of Yinzhihuang injection, using disease models of RA-induced infectious serositis in ducks and heptane-induced inflammation in mice and rats. The duck mortality rate was reduced from 60% to 20% and both the inflammatory response and histological damage were ameliorated by treatment with Yinzhihuang injection (0.02 g/kg). Further studies indicated that superoxide dismutase (SOD), nitric oxide synthase (NOS), and inducible nitric oxide synthase (iNOS) were elevated while malondialdehyde (MDA), nitric oxide (NO) and RA growth were inhibited when the ducks were treated by Yinzhihuang injection. In addition, Yinzhihuang injection (0.04 g/ml) effectively inhibited xylene-induced auricle swelling in mice, (demonstrating an inhibition rate of 35.21%), egg albumen-induced paw metatarsus swelling in rats, (demonstrating an inhibition rate of 22.30%), and agar-induced formation of granulation tissue. These results suggest that Yinzhihuang injection ameliorates RA-induced infectious serositis in ducks by modulation of inflammatory mediators and antioxidation.


Subject(s)
Anti-Bacterial Agents/therapeutic use , Anti-Inflammatory Agents/therapeutic use , Antioxidants/therapeutic use , Drugs, Chinese Herbal/therapeutic use , Ducks/microbiology , Flavobacteriaceae Infections/veterinary , Riemerella/drug effects , Serositis/veterinary , Animals , Anti-Bacterial Agents/administration & dosage , Anti-Inflammatory Agents/administration & dosage , Antioxidants/administration & dosage , Drugs, Chinese Herbal/administration & dosage , Ducks/metabolism , Flavobacteriaceae Infections/drug therapy , Flavobacteriaceae Infections/metabolism , Flavobacteriaceae Infections/microbiology , Injections , Male , Malondialdehyde/metabolism , Mice , Mice, Inbred BALB C , Nitric Oxide Synthase/metabolism , Poultry Diseases/drug therapy , Poultry Diseases/metabolism , Poultry Diseases/microbiology , Rats , Rats, Wistar , Serositis/drug therapy , Serositis/metabolism , Serositis/microbiology , Superoxide Dismutase/metabolism
4.
Inmunología (1987) ; 34(2): 24-26, abr.-jun. 2015. ilus
Article in Spanish | IBECS | ID: ibc-143017

ABSTRACT

Describimos el caso de una paciente con historia de pericarditis recurrentes de 16 años de evolución sin fiebre objetivable ni de patrón recurrente. Se le realizan múltiples estudios tratando de averiguar la etiología de la enfermedad. Finalmente, surge la sospecha de fiebre mediterránea familiar con la confirmación diagnostica de la amplificación genética específica de los exones 2 y 10 del gen MEFV. Destacamos la importancia de la sospecha diagnóstica en casos atípicos (AU)


No disponible


Subject(s)
Adult , Female , Humans , Familial Mediterranean Fever/epidemiology , Familial Mediterranean Fever/immunology , Familial Mediterranean Fever/prevention & control , Diagnosis, Differential , Colchicine/immunology , Colchicine/therapeutic use , Familial Mediterranean Fever/etiology , Medical History Taking , Serositis/immunology , Serositis/microbiology , Electrocardiography/methods , Chest Pain/etiology , Chest Pain , Pleural Effusion/immunology , Pleural Effusion
5.
Curr Microbiol ; 70(5): 631-6, 2015 May.
Article in English | MEDLINE | ID: mdl-25561407

ABSTRACT

Fibrinopurulent polyserositis is of utmost importance in commercial broiler production worldwide. This multifactorial endemic disease is marked by severe clinical alterations post-mortem, yet its effects on food safety and processing hygiene criteria remain unclear. Current considerations presume that bacteraemia lead to meat being unfit for consumption. In the present study, we evaluated some microbiological criteria of affected broiler carcasses in comparison to unaffected control broiler carcasses. The results thereof indicated that the lesions did not result in higher bacterial counts or in an increased percentage of contaminated meat. The carry-over of associated zoonotic pathogens into the food chain seems to be not more prevalent in birds affected with non-systemic affections of polyserositis.


Subject(s)
Bacteria/isolation & purification , Food Safety , Meat/microbiology , Poultry Diseases/microbiology , Serositis/microbiology , Animals , Bacterial Load
6.
Transbound Emerg Dis ; 60(2): 140-2, 2013 Apr.
Article in English | MEDLINE | ID: mdl-22494561

ABSTRACT

From January 2007 to December 2011, a total of 106 Haemophilus parasuis strains isolated from pigs were serotyped by agar gel diffusion test (DG). Serovar 4 was the most prevalent (24.5%), followed by serovar 13 (19.8%) and serovar 5 (11.3%). Twenty-nine strains were non-typeable (27.3%). The strains were divided into two groups, depending on whether they were isolated from specific pathological lesions of systemic disease such as polyserositis, arthritis or meningitis (73 cases of 106) or from the lower respiratory tract of pigs suffering from bronchopneumonia (33 cases of 106). Serovars 4 and 13 had a higher prevalence in systemic infection (polyserositis) than in respiratory disease only. Pasteurella multocida (14/106), Streptococcus suis (7/106), Actinobacillus pleuropneumoniae (4/106), Bordetella bronchiseptica (3/106) and Arcanobacterium pyogenes (3/106) were isolated in association with H. parasuis.


Subject(s)
Haemophilus Infections/veterinary , Haemophilus parasuis/isolation & purification , Swine Diseases/epidemiology , Animals , Arthritis, Infectious/microbiology , Arthritis, Infectious/pathology , Arthritis, Infectious/veterinary , Bronchopneumonia/microbiology , Bronchopneumonia/pathology , Bronchopneumonia/veterinary , Haemophilus Infections/epidemiology , Haemophilus Infections/pathology , Haemophilus parasuis/pathogenicity , Italy/epidemiology , Meningitis, Bacterial/microbiology , Meningitis, Bacterial/pathology , Meningitis, Bacterial/veterinary , Prevalence , Seroepidemiologic Studies , Serositis/microbiology , Serositis/pathology , Serositis/veterinary , Serotyping , Swine , Swine Diseases/microbiology , Swine Diseases/pathology
7.
Rom J Morphol Embryol ; 53(3 Suppl): 835-40, 2012.
Article in English | MEDLINE | ID: mdl-23188450

ABSTRACT

A 41-year-old female patient was admitted into Surgery Clinic accusing abdominal pain, diarrhea, fever and chills. Based on clinical, biological and imaging data, it was established a diagnosis of pelviperitonitis and it was initiated an antibiotic and anti-inflammatory treatment. As fever and abdominal pain continued, it was decided to go on with surgery that revealed suppurated and perforated mesenteric adenopathy. Pus was sampled for bacteriological exam and also biopsy was performed for pathological exam. The result of pathological exam was suggestive for a specific granulomatous lesion (TB lesion). It was established diagnosis of TB mesenteric adenopathy and it was initiated specific anti-TB treatment according to WHO guidelines. After three, respectively five months of treatment, patient developed a right laterocervical adenopathy that fistulized in both cases, despite the correct treatment administered. No resistant TB strain and no atypical mycobacteria was discovered.


Subject(s)
Lymphatic Diseases/microbiology , Serositis/diagnosis , Serositis/microbiology , Tuberculosis/pathology , Adult , Female , Humans , Lymphatic Diseases/pathology , Mesentery
8.
J Bacteriol ; 194(12): 3270-1, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22628503

ABSTRACT

Riemerella anatipestifer is an infectious pathogen causing serositis in ducks. We had the genome of the R. anatipestifer reference strain ATCC 11845 sequenced. The completed draft genome consists of one circular chromosome with 2,164,087 bp. There are 2,101 genes in the draft, and its GC content is 35.01%.


Subject(s)
DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Genome, Bacterial , Riemerella/genetics , Animals , Bird Diseases/microbiology , Ducks , Molecular Sequence Data , Riemerella/isolation & purification , Sequence Analysis, DNA , Serositis/microbiology , Serositis/veterinary
9.
Can J Vet Res ; 76(3): 195-200, 2012 Jul.
Article in English | MEDLINE | ID: mdl-23277698

ABSTRACT

An optimized protocol was developed for the simultaneous detection and differentiation of Haemophilus parasuis, Streptococcus suis, and Mycoplasma hyorhinis in formalin-fixed, paraffin-embedded (FFPE) tissues with multiplex nested polymerase chain reaction (PCR). This method also determines the prevalence of these bacteria in pigs with polyserositis. DNA extraction with a combination of a commercial reagent and proteinase K resulted in more frequent detection of the pathogens than DNA extraction with proteinase K alone. Among FFPE tissue samples from 312 cases of polyserositis in which at least 1 bacterial species was detected, multiplex nested PCR detected H. parasuis in 239 (77%), S. suis in 124 (40%), and M. hyorhinis in 40 (13%). The disease was caused by a single pathogen in 224 (72%) of the cases and multiple pathogens in 88 (28%). Among the pigs positive for H. parasuis, S. suis, and M. hyorhinis by multiplex nested PCR, the pathogen was isolated from only 11%, 35%, and 28%, respectively. Therefore, the PCR protocol developed in this study is a useful diagnostic method when samples are negative after isolation methods and even for samples in which only 1 pathogen was isolated.


Subject(s)
Haemophilus parasuis/isolation & purification , Multiplex Polymerase Chain Reaction/veterinary , Mycoplasma hyorhinis/isolation & purification , Serositis/veterinary , Streptococcus suis/isolation & purification , Swine Diseases/microbiology , Animals , Formaldehyde , Haemophilus Infections/microbiology , Haemophilus Infections/veterinary , Multiplex Polymerase Chain Reaction/methods , Mycoplasma Infections/microbiology , Mycoplasma Infections/veterinary , Paraffin Embedding , Sensitivity and Specificity , Serositis/microbiology , Streptococcal Infections/microbiology , Streptococcal Infections/veterinary , Swine , Swine Diseases/diagnosis , Tissue Fixation
10.
J Vet Med Sci ; 72(9): 1225-7, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20424392

ABSTRACT

The aim of this study was to develop in situ hybridization for detection of Mycoplasma hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from pigs with polyserositis. M. hyorhinis was isolated from the spleen (2 pigs) and pericardium (1 pig). M. hyorhinis DNA was detected 16 out of 20 pigs with polyserositis. In situ hybridization produced a distinct positive signal for the M. hyorhinis p37 gene in inflammatory cells in the polyserositis. In situ hybridization developed in the present study present diagnostic tools capable of detection of M. hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from the naturally infected pigs.


Subject(s)
Mycoplasma Infections/veterinary , Mycoplasma hyorhinis/isolation & purification , Serositis/veterinary , Swine Diseases/microbiology , Animals , Base Pairing , DNA Primers , DNA, Bacterial/isolation & purification , Heart/microbiology , In Situ Hybridization/veterinary , Mycoplasma Infections/diagnosis , Mycoplasma hyorhinis/genetics , Polymerase Chain Reaction , Serositis/microbiology , Swine , Swine Diseases/diagnosis
11.
Can J Vet Res ; 74(4): 314-6, 2010 Oct.
Article in English | MEDLINE | ID: mdl-21197232

ABSTRACT

The objective of this study was to determine the capsular serotypes and potential virulence factors of Streptococcus suis isolated from pigs with polyserositis. Among the 24 isolates evaluated, serotype 3 [7 (29%) of the isolates] and serotype 4 [5 (21%)] were the most common. The isolates were also studied for the presence of the genes mrp, epf, and sly, which encode muramidase-released protein (MRP), extracellular factor (EF), and suilysin (SLY), respectively. Of the 24 isolates, 8 carried mrp: 4 of serotype 3, 2 of serotype 2, and 2 of serotype 4. One mrp(+) isolate (serotype 2) also carried the epf gene. All 24 isolates carried the sly gene. The serotype and genotype distribution greatly differed from that reported for isolates from pigs with other clinical manifestations of S. suis infection in other countries.


Subject(s)
Bacterial Capsules/metabolism , Serositis/veterinary , Streptococcal Infections/veterinary , Streptococcus suis/pathogenicity , Swine Diseases/microbiology , Agglutination Tests/veterinary , Animals , Antigens, Bacterial/genetics , Antigens, Bacterial/metabolism , Bacterial Capsules/genetics , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Hemolysin Proteins/genetics , Hemolysin Proteins/metabolism , Polymerase Chain Reaction/veterinary , Serositis/microbiology , Serotyping/veterinary , Streptococcal Infections/immunology , Streptococcal Infections/microbiology , Streptococcus suis/genetics , Swine , Swine Diseases/immunology , Virulence
12.
Rev Port Pneumol ; 15(4): 721-7, 2009.
Article in Portuguese | MEDLINE | ID: mdl-19547902

ABSTRACT

It is a case report of young female that had diagnostic criteria of Systemic Lupus Erithematosus in activity, according American Rheumatology Association. The patient had fever, anemia, arthritis, cellular casts, positive LE cells, positive antinuclear antibody. She has evolved to bilateral pleural effusion and pericardic effusion that both have been initially attributed to lupus. Due to she has also maintained low fever, sudoresis, loss of weight and a persistent serositis, a thoracocenthesis with pleural biopsy has been done and the result of it has revealed granulomatous chronic pleuritis, diagnosticing pleural tuberculosis. A pericardiocenthesis has also been done. After six months of anti -tuberculosis therapy, there was a regression of radiologic imaging. Some concepts referring to tuberculosis and systemic lupus erythematosus are discussed, including symptoms, diagnosis and specific situations. It is emphasized the necessity of early diagnosis and appropriate management of tuberculosis disease in lupic patients, in areas where tuberculosis is endemic.


Subject(s)
Lupus Erythematosus, Systemic/complications , Serositis/microbiology , Tuberculosis, Pleural/complications , Adolescent , Female , Humans
13.
Trans R Soc Trop Med Hyg ; 103(11): 1180-2, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19251293

ABSTRACT

Salmonella enterica serovar Enteritidis (S. Enteritidis), a non-typhoid Salmonella, is an important emerging pathogen that usually causes gastroenteritis. Here, we report polyserositis with right-sided pleural effusion and loculated collection of fluid in the peritoneum caused by S. Enteritidis in a 60-year-old man from southern India. The patient was immunocompetent and did not have preceding gastroenteritis or any local structural abnormality. Malnutrition and old age might have been the predisposing factors. The patient received intravenous ceftriaxone for 2 weeks followed by oral ciprofloxacin. Pleurocentesis and abdominal paracentesis were also done. The patient was cured as evidenced by clinical improvement and radiological disappearance of the fluid collection.


Subject(s)
Pleural Effusion/microbiology , Salmonella Infections/microbiology , Salmonella enteritidis/isolation & purification , Serositis/microbiology , Humans , India , Male , Malnutrition/complications , Middle Aged
15.
Ultrastruct Pathol ; 31(6): 373-7, 2007.
Article in English | MEDLINE | ID: mdl-18098054

ABSTRACT

Bartonella spp. constitute emerging pathogens of worldwide distribution. Bacillary angiomatosis is the most frequent skin manifestation of bartonelloses; nevertheless, B. henselae infection should always be considered systemic, especially in immunodeficient individuals. The authors report the case of an AIDS patient with bacillary angiomatosis, who had concurrent severe anemia, hepatitis, peritonitis, pleuritis, and pericarditis. Clinical manifestation, electronic microscopic examination of erythrocytes, and histopathology of a papule biopsy suggested a Bartonella sp. infection. Multiple genes were target by PCR and B. henselae DNA was amplified and sequenced (GenBank accession number EF196804) from the angiomatous papule. Treatment with clarithromycin resulted in resolution of the bacillary angiomatosis, fever, anemia, panserosites, and hepatitis.


Subject(s)
Anemia/microbiology , Angiomatosis, Bacillary/microbiology , Bartonella henselae/isolation & purification , HIV Infections/microbiology , Hepatitis/microbiology , Serositis/microbiology , Skin Diseases/microbiology , AIDS-Related Opportunistic Infections/drug therapy , AIDS-Related Opportunistic Infections/microbiology , AIDS-Related Opportunistic Infections/pathology , Adult , Anemia/drug therapy , Anemia/pathology , Angiomatosis, Bacillary/drug therapy , Angiomatosis, Bacillary/pathology , Anti-Bacterial Agents/therapeutic use , Bartonella henselae/genetics , Base Sequence , Clarithromycin/therapeutic use , DNA, Bacterial/analysis , Erythrocytes/microbiology , Erythrocytes/ultrastructure , Hepatitis/drug therapy , Hepatitis/pathology , Humans , Immunocompromised Host , Male , Microscopy, Electron, Transmission/methods , Molecular Sequence Data , Serositis/drug therapy , Serositis/pathology , Skin Diseases/drug therapy , Skin Diseases/pathology , Treatment Outcome
16.
Scand J Infect Dis ; 39(10): 911-3, 2007.
Article in English | MEDLINE | ID: mdl-17852886

ABSTRACT

We report a case of laboratory-acquired meningococcal disease in a 31-y-old female research assistant. The clinical presentation of the case was atypical with polyserositis affecting knees, pleura and pericardium, without septicaemia or meningitis. The diagnosis was made by positive PCR for Neisseria meningitidis (genogroup C, genosubtype P1.7, 16, 35 and without mutations of the penA gene) in the patient's right knee. Serology confirmed the diagnosis after recovery. This case had an atypical clinical picture, exemplifies the use of non-culture methods for diagnosis and characterization, and reminds us about the importance of safe routines for the laboratory work.


Subject(s)
Laboratory Infection , Medical Laboratory Personnel , Meningococcal Infections , Neisseria meningitidis, Serogroup C/isolation & purification , Serositis , Adult , Female , Humans , Knee Joint/microbiology , Laboratory Infection/diagnosis , Laboratory Infection/microbiology , Laboratory Infection/physiopathology , Meningococcal Infections/diagnosis , Meningococcal Infections/microbiology , Meningococcal Infections/physiopathology , Neisseria meningitidis, Serogroup C/classification , Neisseria meningitidis, Serogroup C/genetics , Serositis/diagnosis , Serositis/microbiology , Serositis/physiopathology
17.
Vet Rec ; 151(17): 502-5, 2002 Oct 26.
Article in English | MEDLINE | ID: mdl-12430998

ABSTRACT

The efficacy of a new Haemophilus parasuis vaccine for pigs was investigated. The vaccine contains H parasuis serotype 5 cells and is adjuvanted with Diluvac Forte (Intervet). Groups of pigs were vaccinated at five and seven weeks with 2 ml and their littermates served as unvaccinated controls. The vaccinated pigs were protected against a challenge with another strain of Hparasuis serotype 5 at two, eight and 17 weeks after the second vaccination, whereas the controls became very ill. The susceptibility of the pigs to the infection decreased with increasing age. After a heterologous challenge with H parasuis serotypes 1, 12, 13 and 14, two weeks after the second vaccination, the vaccine also gave clear protection. The severity of the illness among the control pigs differed with the different serotypes.


Subject(s)
Haemophilus Infections/veterinary , Haemophilus Vaccines , Haemophilus/immunology , Serositis/veterinary , Swine Diseases/prevention & control , Adjuvants, Immunologic/administration & dosage , Animals , Haemophilus/classification , Haemophilus/pathogenicity , Haemophilus Infections/microbiology , Haemophilus Infections/pathology , Haemophilus Infections/prevention & control , Random Allocation , Serositis/microbiology , Serositis/prevention & control , Serotyping/veterinary , Severity of Illness Index , Swine , Swine Diseases/microbiology , Swine Diseases/pathology , Treatment Outcome , Vaccines, Inactivated
18.
Vet Rec ; 151(1): 18-21, 2002 Jul 06.
Article in English | MEDLINE | ID: mdl-12137419

ABSTRACT

Ten pregnant gilts were divided into two groups of five and one group was vaccinated at 80 and 95 days of pregnancy with a commercial bacterin containing Haemophilus parasuis serovars 2, 3 and 5. Half the piglets born to each group of gilts were vaccinated at seven and 21 days of age with the same bacterin, and one week after they were weaned at five weeks, all the piglets were inoculated intratracheally with 10(6) colony-forming units of Hparasuis serovar 5. At slaughter, a significantly smaller percentage of the lungs of the pigs born to the vaccinated gilts was affected by pneumonic lesions, and significantly fewer of them had arthritic joint changes. The average daily liveweight gain of the pigs born to the vaccinated gilts was significantly greater than that of those born to the unvaccinated gilts, but the vaccination of the piglets had no effect. There was no significant difference between the feed conversion ratios of the four groups of piglets, and none between the average times they took to reach slaughter weight. The pigs born to the vaccinated gilts had higher ELISA titres to Hparasuis than those born to the unvaccinated gilts.


Subject(s)
Arthritis/veterinary , Haemophilus Infections/veterinary , Serositis/veterinary , Swine Diseases/prevention & control , Vaccination/veterinary , Animals , Animals, Newborn , Antibodies, Bacterial/analysis , Arthritis/microbiology , Arthritis/prevention & control , Enzyme-Linked Immunosorbent Assay , Female , Haemophilus Infections/microbiology , Haemophilus Infections/prevention & control , Lung/microbiology , Lung/pathology , Male , Pregnancy , Serositis/microbiology , Serositis/prevention & control , Swine , Swine Diseases/microbiology
19.
FEMS Microbiol Lett ; 179(1): 11-9, 1999 Oct 01.
Article in English | MEDLINE | ID: mdl-10481080

ABSTRACT

Riemerella anatipestifer is the causative agent of polyserositis of ducks and geese. We have previously reported that a 3.9-kb plasmid, pCFC1, carries protein genes (vapD1 and vapD2) that are similar to virulence-associated genes of other bacteria. In the present study, we report the complete sequence of a second plasmid of 5.6 kb, pCFC2. pCFC2 has a 28% G-C content and three large open reading frames (ORFs). One of the ORFs (designated asVapD1) encodes a polypeptide that shares 53.9, 53.9, 48.3, 48.3 and 46.1% identity with virulence-associated proteins of Dichelobacter nodosus, Actinobacillus actinomycetemcomitans, Neisseria gonorrhoeae, Helicobacter pylori and Haemophilus influenzae, respectively. The second ORF encodes a putative DNA replication protein (RepA3) with 309 amino acids and a molecular mass of approximately 36 kDa. A novel insertion sequence (IS) element, designated ISRa1, was found on the plasmid pCFC2. ISRa1 was flanked by 15-bp imperfect inverted repeats (only one mismatched nucleotide). ISRa1 contained an ORF encoding a putative transposase of 292 amino acids. Southern blot analysis indicated that in R. anatipestifer strains examined, ISRa1 was present with 2-20 copies (at least). ISRa1 displayed a sequence approximately 35% homologous to the putative IS982 and RSBst-alpha from Lactococcus lactis ssp. cremoris SK11 and Bacillus stearothermophilus CU21. Three hybridization patterns of genomic DNA of eight R. anatipestifer strains with an ISRa1 probe indicated that ISRa1 might be a useful tool for epidemiological studies.


Subject(s)
Gram-Negative Bacteria/genetics , Gram-Negative Bacteria/pathogenicity , Plasmids , Poultry Diseases/microbiology , Serositis/veterinary , Amino Acid Sequence , Animals , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Base Sequence , Ducks , Geese , Gram-Negative Bacterial Infections/microbiology , Gram-Negative Bacterial Infections/veterinary , Membrane Glycoproteins/chemistry , Membrane Glycoproteins/genetics , Molecular Sequence Data , Restriction Mapping , Sequence Alignment , Sequence Homology, Amino Acid , Serositis/microbiology , Virulence/genetics
20.
Med Microbiol Immunol ; 187(2): 71-8, 1998 Oct.
Article in English | MEDLINE | ID: mdl-9832320

ABSTRACT

To characterize the role of specific lymphocyte subsets in Chlamydia trachomatis infection, we established a murine model using the mouse pneumonitis agent (MoPn) of C. trachomatis and C.B-17 scid/scid (SCID) mice which lack functional B and T cells. After intraperitoneal inoculation with the bacteria, SCID mice developed polyserositis with pleuritis, pericarditis, and perihepatitis. Within 8 weeks post infection, SCID mice succumbed to the disease, whereas immunocompetent congenic C.B-17+/+ mice resolved the infection. Adoptive transfer of immune spleen cells into MoPn-infected SCID mice resulted in a complete elimination of the agent and prevention of polyserositis as measured by quantitative chlamydial culture, direct immunofluorescence and histopathological analysis. Selective reconstitution of MoPn-infected SCID mice with immune B lymphocytes, CD4+ T cells or CD8+ T cells alone did not influence the chlamydial load in the lung and liver of infected SCID animals, resulting in a polyserositis as observed in untreated MoPn-infected SCID mice. However, co-transfer of both CD4+ T cells and CD8+ T cells led to a significant reduction of chlamydiae in quantitative organ culture coupled with unremarkable histopathology. These data confirm that T cell-mediated immune responses are essential for immune protection in chlamydial infection, although total eradication of the agent could not be achieved. Further experiments are needed to stress the importance of a concerted action of B and T lymphocytes, as indicated by the complete protective efficacy of transferred splenocytes.


Subject(s)
CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Chlamydia Infections/immunology , Chlamydia trachomatis , Serositis/immunology , Adoptive Transfer , Animals , Antibodies, Bacterial/blood , Antibody Specificity , B-Lymphocytes/transplantation , Blotting, Western , CD4-Positive T-Lymphocytes/transplantation , CD8-Positive T-Lymphocytes/transplantation , Cell Line , Chlamydia Infections/pathology , Chlamydia Infections/prevention & control , Chlamydia trachomatis/immunology , Female , Fluorescent Antibody Technique, Indirect , Immunohistochemistry , Liver/immunology , Liver/microbiology , Lung/immunology , Lung/microbiology , Male , Mice , Mice, SCID , Serositis/microbiology , Serositis/pathology , Specific Pathogen-Free Organisms
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