Subject(s)
Herpesvirus 4, Human , Infectious Mononucleosis/complications , Serositis/virology , Serous Membrane/virology , Acute Disease , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Infant , Infectious Mononucleosis/diagnosis , Male , Middle Aged , Young AdultABSTRACT
Peritonitis of viral etiology is rarely reported in the literature; a prior report described a patient undergoing continuous ambulatory peritoneal dialysis who had the disease. We report a case of primary herpetic peritonitis (the agent of which was typed by polymerase chain reaction as herpes simplex virus biotype 1), which caused intestinal perforation, and we review the current literature and provide possible pathophysiologic mechanisms.
Subject(s)
Herpes Simplex/complications , Herpesvirus 1, Human , Intestinal Perforation/etiology , Peritonitis/virology , Female , Herpesvirus 1, Human/isolation & purification , Humans , Immunoenzyme Techniques , Inclusion Bodies, Viral , Middle Aged , Peritonitis/complications , Serous Membrane/virologyABSTRACT
The prevalence and cellular distribution of human herpesvirus 7 (HHV-7) in archival labial salivary glands was analysed for virus-specific DNA sequences by polymerase chain reaction (PCR) and in situ hybridization signals. In addition, the cellular expression of HHV-7-encoded protein was detected by immunohistochemical staining with a virus-specific monoclonal antibody. Eleven of 20 samples were positive for the HHV-7 DNA sequence by PCR. Eighteen of 20 tissues analysed by in situ hybridization showed signals in ductal, serous and mucous cells. Some nuclei of these cells and also the myoepithelial population were positive. In immunolocalization studies, all 20 salivary glands consistently showed HHV-7-expressed protein in the cytoplasm of ductal cuboidal and columnar cells. The protein was also found in the cytoplasm of mucous and serous acinar cells that were immunopositive for HHV-7. The observations are consistent with the suggestion that the labial salivary gland is a site for virus replication, potential persistence and a source of infective HHV-7 in saliva.
Subject(s)
Herpesvirus 7, Human/isolation & purification , Salivary Glands/virology , Adolescent , Adult , Antibodies, Monoclonal , Blotting, Southern , Cell Nucleus/ultrastructure , Cell Nucleus/virology , Child , Cytoplasm/ultrastructure , Cytoplasm/virology , DNA, Viral/analysis , Epithelial Cells/cytology , Epithelial Cells/virology , Female , Gene Expression Regulation, Viral , Herpesviridae Infections , Herpesvirus 7, Human/genetics , Humans , Immunohistochemistry , In Situ Hybridization , Male , Mucous Membrane/cytology , Mucous Membrane/virology , Polymerase Chain Reaction , Saliva/virology , Salivary Ducts/cytology , Salivary Ducts/virology , Salivary Glands/cytology , Sequence Analysis, DNA , Serous Membrane/cytology , Serous Membrane/virology , Viral Proteins/analysis , Viral Proteins/genetics , Virus Latency , Virus ReplicationABSTRACT
Reports connect Kaposi's sarcoma-associated herpesvirus (KSHV) with various types of Kaposi's sarcoma (KS) and body-cavity-based lymphomas (primary effusion lymphomas). KSHV DNA sequences have also been detected in a few non-KS malignant vascular tumors. To determine whether KSHV is associated with malignant vascular tumors involving the body cavities, 11 primary vascular tumors of the serous membranes (4 angiosarcomas, 7 hemangioendotheliomas) were tested for KSHV sequences by polymerase chain reaction and Southern blot hybridization. The tumors were from patients who were not known to be immunocompromised. KSHV sequences were detected in the penile KS control but were absent in all 11 specimens studied. Our results were consistent with previous reports that KSHV sequences were rarely detected in non-KS vascular lesions. The results also suggested that although KSHV sequences might be present in primary effusion tumors such as primary effusion lymphoma, they might not be found in other tumors related to body cavities or to serous membranes.