ABSTRACT
OBJECTIVE: The study aims to evaluate the effect of a glass ionomer cement (GIC; Fuji 9 Gold Label, GC) with added calcium orthophosphate particles and a calcium silicate cement (CSC; Biodentine, Septodont) regarding ion release, degradation in water, mineral content, and mechanical properties of demineralized dentin samples. METHODS: GIC, GIC + 5% DCPD (dicalcium phosphate dihydrate), GIC + 15% DCPD, GIC + 5% ß-TCP (tricalcium phosphate), GIC + 15% ß-TCP (by mass), and CSC were evaluated for Ca2+/Sr2+/F- release in water for 56 days. Cement mass loss was evaluated after 7-day immersion in water. Partially demineralized dentin disks were kept in contact with materials while immersed in simulated body fluid (SBF) at 37 °C for 56 days. The "mineral-to-matrix ratio" (MMR) was determined by ATR-FTIR spectroscopy. Dentin hardness and elastic modulus were obtained by nanoindentation. Samples were observed under scanning and transmission electron microscopy. Data were analyzed by ANOVA/Tukey test (α = 0.05). RESULTS: Ca2+ release from CSC and GIC (µg/cm2) were 4737.0 ± 735.9 and 13.6 ± 1.6, respectively. In relation to the unmodified GIC, the addition of DCPD or ß-TCP increased ion release (p < 0.001). Only the dentin disks in contact with CSC presented higher MMR (p < 0.05) and mechanical properties than those restored with a resin composite used as control (p < 0.05). Mass loss was similar for GIC and CSC; however, the addition of DCPD or ß-TCP increased GIC degradation (p < 0.05). CONCLUSION: Despite the increase in ion release, the additional Ca2+ sources did not impart remineralizing capability to GIC. Both unmodified GIC and CSC showed similar degradation in water. CLINICAL RELEVANCE: CSC was able to promote dentin remineralization.
Subject(s)
Calcium Compounds , Calcium Phosphates , Calcium , Glass Ionomer Cements , Silicates , Glass Ionomer Cements/pharmacology , Glass Ionomer Cements/chemistry , Calcium/analysis , Phosphates/analysis , Silicate Cement/analysis , Silicate Cement/pharmacology , Dentin , Water/chemistry , Materials TestingABSTRACT
AIM: To verify the in vitro cytocompatibility of iRoot BP Plus (iRoot) and to compare it with White ProRoot MTA (MTA). METHODOLOGY: Thirty-six human maxillary incisor root canals were prepared using a step-back flaring technique. The apical 3 mm was resected perpendicular to the long axis at the roots, and root-end cavities were prepared with the aid of an ultrasonic device plus a diamond retrotip with continuous irrigation using water, producing standardized preparations. After that, the root-end cavities were filled with iRoot or MTA, and each root was exposed to cell culture media for 24 or 48 h. Human osteoblast cells were exposed to the extracts thus obtained, and a multiparametric cell viability assay was performed, evaluating mitochondrial activity, membrane integrity and cell density. The results were analysed by one-way analysis of variance, complemented with the Duncan post-test (P < 0.05). RESULTS: Cells exposed to MTA revealed a cytocompatibility pattern similar to the untreated cells (negative control), at both experimental times (P > 0.05). iRoot, however, promoted a significantly poorer viability than MTA and the control, after 48 h of exposure (P < 0.001). Nevertheless, iRoot did not induce critical cytotoxic effects because cell viability remained higher than 70% of the control group in most tests performed. CONCLUSION: iRoot and MTA were biocompatible and did not induce critical cytotoxic effects.
Subject(s)
Biocompatible Materials/pharmacology , Calcium Compounds/pharmacology , Osteoblasts/drug effects , Silicate Cement/pharmacology , Silicates/pharmacology , Aluminum Compounds/pharmacology , Cell Count , Cell Culture Techniques , Cell Membrane/drug effects , Cell Shape/drug effects , Cell Survival/drug effects , Culture Media, Conditioned , Drug Combinations , Humans , Mitochondria/drug effects , Oxides/pharmacology , Retrograde Obturation/methods , Root Canal Preparation/methods , Time Factors , Tissue Culture Techniques , Zinc Oxide-Eugenol Cement/toxicityABSTRACT
Os autores ralizaram uma investigaçäo clínica para avaliar a capacidade de inibiçäo de cáries do cimento de sílico-fosfato em comparaçäo com o amálgama. Foram realizadas restauraçöes de classe I nos pré-molares de 30 pacientes que apresentavam cáries incipientes oclusais, exclusivamente. Os pacientes estäo sendo controlados através de radiografias interproximais e clinicamente para verificar o aparecimento de novas lesöes cariosas. Nos resultados de 1 ano näo se observou diferença significante entre os materiais testados