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1.
Cerebrovasc Dis ; 50(3): 347-355, 2021.
Article in English | MEDLINE | ID: mdl-33730735

ABSTRACT

INTRODUCTION: Sodium MRI (23Na MRI) derived biomarkers such as tissue sodium concentration (TSC) provide valuable information on cell function and brain tissue viability and has become a reliable tool for the assessment of brain tumors and ischemic stroke beyond pathoanatomical morphology. Patients with major stroke often suffer from different degrees of underlying white matter lesions (WMLs) attributed to chronic small vessel disease. This study aimed to evaluate the WM TSC in patients with an acute ischemic stroke and to correlate the TSC with the extent of small vessel disease. Furthermore, the reliability of relative TSC (rTSC) compared to absolute TSC in these patients was analyzed. METHODOLOGY: We prospectively examined 62 patients with acute ischemic stroke (73 ± 13 years) between November 2016 and August 2019 from which 18 patients were excluded and thus 44 patients were evaluated. A 3D 23Na MRI was acquired in addition to a T2-TIRM and a diffusion-weighted image. Coregistration and segmentation were performed with SPM 12 based on the T2-TIRM image. The extension of WM T2 hyperintense lesions in each patient was classified using the Fazekas scale of WMLs. The absolute TSC in the WM region was correlated to the Fazekas grades. The stroke region was manually segmented on the coregistered absolute diffusion coefficient image and absolute, and rTSC was calculated in the stroke region and compared to nonischemic WM region. Statistical significance was evaluated using the Student t-test. RESULTS: For patients with Fazekas grade I (n = 25, age: 68.5 ± 15.1 years), mean TSC in WM was 55.57 ± 7.43 mM, and it was not statistically significant different from patients with Fazekas grade II (n = 7, age: 77.9 ± 6.4 years) with a mean TSC in WM of 53.9 ± 6.4 mM, p = 0.58. For patients with Fazekas grade III (n = 9, age: 81.4 ± 7.9 years), mean TSC in WM was 68.7 ± 10.5 mM, which is statistically significantly higher than the TSC in patients with Fazekas grade I and II (p < 0.001 and p = 0.05, respectively). There was a positive correlation between the TSC in WM and the Fazekas grade with r = 0.48 p < 0.001. The rTSC in the stroke region was statistically significant difference between low (0 and I) and high (2 and 3) Fazekas grades (p = 0.0353) whereas there was no statistically significant difference in absolute TSC in the stroke region between low (0 and I) and high (2 and 3) Fazekas grades. CONCLUSION: The significant difference in absolute TSC in WM in patients with severe small vessel disease; Fazekas grade 3 can lead to inaccuracies using rTSC quantification for evaluation of acute ischemic stroke using 23 Na MRI. The study, therefore, emphasizes the importance of absolute tissue sodium quantification.


Subject(s)
Cerebral Small Vessel Diseases/diagnostic imaging , Ischemic Stroke/diagnostic imaging , Leukoencephalopathies/diagnostic imaging , Magnetic Resonance Imaging , Sodium Isotopes/metabolism , White Matter/diagnostic imaging , Aged , Aged, 80 and over , Cerebral Small Vessel Diseases/metabolism , Diffusion Magnetic Resonance Imaging , Female , Humans , Image Interpretation, Computer-Assisted , Ischemic Stroke/metabolism , Leukoencephalopathies/metabolism , Male , Middle Aged , Predictive Value of Tests , Prospective Studies , Reproducibility of Results , White Matter/metabolism
2.
J Magn Reson Imaging ; 50(2): 435-444, 2019 08.
Article in English | MEDLINE | ID: mdl-30681221

ABSTRACT

BACKGROUND: Triple-quantum (TQ) filtered sequences have become more popular in sodium MR due to the increased usage of scanners with field strengths exceeding 3T. Disagreement as to whether TQ signal can provide separation of intra- and extracellular compartments persists. PURPOSE: To provide insight into TQ signal behavior on a cellular level. STUDY TYPE: Prospective. PHANTOM/SPECIMEN: Cell-phantoms in the form of liposomes, encapsulated 0 mM, 145 mM, 154 mM Na+ in a double-lipid membrane similar to cells. Poly(lactic-co-glycolic acid) nanoparticles encapsulated 154 mM Na+ within a single-layer membrane structure. Two microcavity chips with each 6 × 106 human HEP G2 liver cells were measured in an MR-compatible bioreactor. FIELD STRENGTH/SEQUENCE: Spectroscopic TQ sequence with time proportional phase-increments at 9.4T. ASSESSMENT: The TQ signal of viable, dead cells, and cell-phantoms was assessed by a fit in the time domain and by the amplitude in the frequency domain. STATISTICAL TESTS: The noise variance (σ) was evaluated to express the deviation of the measured TQ signal amplitude from noise. RESULTS: TQ signal >20σ was found for liposomes encapsulating sodium ions. Liposomal encapsulation of 0 mM Na+ and 154 mM Na+ encapsulation in the nanoparticles resulted in <2σ TQ signal. Cells under normal perfusion resulted in >9σ TQ signal. Compared with TQ signal under normal perfusion, a 56% lower TQ signal of was observed (25σ) during perfusion stop. TQ signal returned to 92% of the initial signal after reperfusion. DATA CONCLUSION: Our measurements indicate that TQ signal in liposomes was observed due to the trapping of ions within the double-lipid membrane rather than from the intraliposomal space. Transfer to the cell results suggests that TQ signal was observed from motion restriction equivalent to trapping. LEVEL OF EVIDENCE: 1 Technical Efficacy: Stage 3 J. Magn. Reson. Imaging 2019;50:435-444.


Subject(s)
Liposomes/metabolism , Liver/metabolism , Magnetic Resonance Spectroscopy/methods , Nanoparticles/metabolism , Signal Processing, Computer-Assisted , Sodium Isotopes/metabolism , Cell Survival , Cells, Cultured , Humans , In Vitro Techniques , Prospective Studies
3.
Kidney Int ; 93(5): 1191-1197, 2018 05.
Article in English | MEDLINE | ID: mdl-29455909

ABSTRACT

Long-term elevated blood sugar levels result in tissue matrix compositional changes in patients with diabetes mellitus type 2 (T2DM). We hypothesized that hemodialysis patients with T2DM might accumulate more tissue sodium than control hemodialysis patients. To test this, 23Na magnetic resonance imaging (23Na MRI) was used to estimate sodium in skin and muscle tissue in hemodialysis patients with or without T2DM. Muscle fat content was estimated by 1H MRI and tissue sodium content by 23Na MRI pre- and post-hemodialysis in ten hemodialysis patients with T2DM and in 30 matched control hemodialysis patients. We also assessed body fluid distribution with the Body Composition Monitor. 1H MRI indicated a tendency to higher muscle fat content in hemodialysis patients with T2DM compared to non-diabetic hemodialysis patients. 23Na MRI indicated increased sodium content in muscle and skin tissue of hemodialysis patients with T2DM compared to control hemodialysis patients. Multi-frequency bioimpedance was used to estimate extracellular water (ECW), and excess ECW in T2DM hemodialysis patients correlated with HbA1c levels. Sodium mobilization during hemodialysis lowered muscle sodium content post-dialysis to a greater degree in T2DM hemodialysis patients than in control hemodialysis patients. Thus, our findings provide evidence that increased sodium accumulation occurs in hemodialysis patients with T2DM and that impaired serum glucose metabolism is associated with disturbances in tissue sodium and water content.


Subject(s)
Body Composition , Diabetes Mellitus, Type 2/diagnostic imaging , Diabetic Nephropathies/therapy , Magnetic Resonance Imaging , Muscle, Skeletal/diagnostic imaging , Radiopharmaceuticals/metabolism , Renal Dialysis , Skin/diagnostic imaging , Sodium Isotopes/metabolism , Adiposity , Aged , Blood Glucose/metabolism , Body Fluid Compartments/diagnostic imaging , Body Fluid Compartments/metabolism , Case-Control Studies , Diabetes Mellitus, Type 2/metabolism , Diabetes Mellitus, Type 2/physiopathology , Diabetic Nephropathies/diagnostic imaging , Diabetic Nephropathies/metabolism , Diabetic Nephropathies/physiopathology , Electric Impedance , Female , Homeostasis , Humans , Male , Middle Aged , Muscle, Skeletal/metabolism , Muscle, Skeletal/physiopathology , Predictive Value of Tests , Skin/metabolism , Skin/physiopathology , Tissue Distribution
4.
J Food Sci ; 82(7): 1563-1568, 2017 Jul.
Article in English | MEDLINE | ID: mdl-28556249

ABSTRACT

The effects of formulation and processing parameters on sodium availability in a model lipid/protein-based emulsion gel were studied for purposes of sodium reduction. Heat-set model gels were prepared with varying levels of protein, lipid, and NaCl contents and high pressure homogenization treatments. Single quantum and double quantum-filtered 23 Na NMR spectroscopy experiments were used to characterize sodium mobility, structural order around "bound" (restricted mobility) sodium, and sodium binding, which have been correlated to saltiness perception in food systems previously. Total sodium mobility was lower in gels with higher protein or fat content, and was not affected by changes in homogenization pressure. The gels with increased protein, fat, or homogenization pressure had increased structure surrounding "bound" sodium and more relative "bound" sodium due to increased interfacial protein interactions. The data obtained in this study provide information on factors affecting sodium availability, which can be applied towards sodium reduction in lipid/protein-based foods.


Subject(s)
Emulsions/chemistry , Magnetic Resonance Spectroscopy/methods , Sodium Isotopes/metabolism , Sodium/metabolism , Diet, Sodium-Restricted , Gels/chemistry , Ions , Lipids , Sodium Chloride/chemistry , Sodium, Dietary , Taste
5.
Mult Scler ; 22(8): 1040-7, 2016 07.
Article in English | MEDLINE | ID: mdl-26453681

ABSTRACT

BACKGROUND: Advanced magnetic resonance imaging (MRI) techniques provide a window into pathological processes in multiple sclerosis (MS). Nevertheless, to date only few studies have performed sodium MRI in MS. OBJECTIVES: We analysed total sodium concentration (TSC) in hyperacute, acute and chronic lesions in MS with (23)Na MRI. METHODS: (23)Na MRI and (1)H MRI were performed in 65 MS patients and 10 healthy controls (HC). Mean TSC was quantified in all MS lesions with a diameter of >5 mm and in the normal appearing white and grey matter (NAWM, NAGM). RESULTS: TSC in the NAWM and the NAGM of MS patients was significantly higher compared to HC (WM: 37.51 ± 2.65 mM versus 35.17 ± 3.40 mM; GM: 43.64 ± 2.75 mM versus 40.09 ± 4.64 mM). Acute and chronic MS lesions showed elevated TSC levels of different extent (contrast-enhancing lesions (49.07 ± 6.99 mM), T1 hypointense lesions (45.06 ± 6.26 mM) and remaining T1 isointense lesions (39.88 ± 5.54 mM)). However, non-enhancing hyperacute lesions with a reduced apparent diffusion coefficient showed a TSC comparable to the NAWM (37.22 ± 4.62 mM). CONCLUSIONS: TSC is not only a sensitive marker of the severity of chronic tissue abnormalities in MS but is also highly sensitive to opening of the blood-brain barrier and vasogenic tissue oedema in contrast-enhancing lesions.


Subject(s)
Diffusion Magnetic Resonance Imaging/methods , Gray Matter/diagnostic imaging , Multiple Sclerosis/diagnostic imaging , Sodium Isotopes/metabolism , White Matter/diagnostic imaging , Adolescent , Adult , Case-Control Studies , Contrast Media/administration & dosage , Cross-Sectional Studies , Female , Gray Matter/metabolism , Gray Matter/pathology , Humans , Male , Middle Aged , Multiple Sclerosis/metabolism , Multiple Sclerosis/pathology , Predictive Value of Tests , Sodium Isotopes/administration & dosage , White Matter/metabolism , White Matter/pathology , Young Adult
6.
Magn Reson Med ; 75(4): 1605-16, 2016 Apr.
Article in English | MEDLINE | ID: mdl-25989746

ABSTRACT

PURPOSE: To reduce noise and artifacts in (23)Na MRI with a Compressed Sensing reconstruction and a learned dictionary as sparsifying transform. METHODS: A three-dimensional dictionary-learning compressed sensing reconstruction algorithm (3D-DLCS) for the reconstruction of undersampled 3D radial (23)Na data is presented. The dictionary used as the sparsifying transform is learned with a K-singular-value-decomposition (K-SVD) algorithm. The reconstruction parameters are optimized on simulated data, and the quality of the reconstructions is assessed with peak signal-to-noise ratio (PSNR) and structural similarity (SSIM). The performance of the algorithm is evaluated in phantom and in vivo (23)Na MRI data of seven volunteers and compared with nonuniform fast Fourier transform (NUFFT) and other Compressed Sensing reconstructions. RESULTS: The reconstructions of simulated data have maximal PSNR and SSIM for an undersampling factor (USF) of 10 with numbers of averages equal to the USF. For 10-fold undersampling, the PSNR is increased by 5.1 dB compared with the NUFFT reconstruction, and the SSIM by 24%. These results are confirmed by phantom and in vivo (23)Na measurements in the volunteers that show markedly reduced noise and undersampling artifacts in the case of 3D-DLCS reconstructions. CONCLUSION: The 3D-DLCS algorithm enables precise reconstruction of undersampled (23)Na MRI data with markedly reduced noise and artifact levels compared with NUFFT reconstruction. Small structures are well preserved.


Subject(s)
Brain/diagnostic imaging , Imaging, Three-Dimensional/methods , Machine Learning , Magnetic Resonance Imaging/methods , Sodium Isotopes/chemistry , Adult , Algorithms , Female , Humans , Male , Phantoms, Imaging , Sodium Isotopes/administration & dosage , Sodium Isotopes/metabolism , Young Adult
7.
Clin Exp Immunol ; 168(1): 125-34, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22385247

ABSTRACT

Pulmonary oedema is a hallmark of acute lung injury (ALI), consisting of various degrees of water and proteins. Physiologically, sodium enters through apical sodium channels (ENaC) and is extruded basolaterally by a sodium-potassium-adenosine-triphosphatase pump (Na(+) /K(+) -ATPase). Water follows to maintain iso-osmolar conditions and to keep alveoli dry. We postulated that the volatile anaesthetic sevoflurane would impact oedema resolution positively in an in-vitro and in-vivo model of ALI. Alveolar epithelial type II cells (AECII) and mixed alveolar epithelial cells (mAEC) were stimulated with 20 µg/ml lipopolysaccharide (LPS) and co-exposed to sevoflurane for 8 h. In-vitro active sodium transport via ENaC and Na(+) /K(+) -ATPase was determined, assessing (22) sodium and (86) rubidium influx, respectively. Intratracheally applied LPS (150 µg) was used for the ALI in rats under sevoflurane or propofol anaesthesia (8 h). Oxygenation index (PaO(2) /FiO(2) ) was calculated and lung oedema assessed determining lung wet/dry ratio. In AECII LPS decreased activity of ENaC and Na(+) /K(+) -ATPase by 17·4% ± 13·3% standard deviation and 16·2% ± 13·1%, respectively. These effects were reversible in the presence of sevoflurane. Significant better oxygenation was observed with an increase of PaO(2) /FiO(2) from 189 ± 142 mmHg to 454 ± 25 mmHg after 8 h in the sevoflurane/LPS compared to the propofol/LPS group. The wet/dry ratio in sevoflurane/LPS was reduced by 21·6% ± 2·3% in comparison to propofol/LPS-treated animals. Sevoflurane has a stimulating effect on ENaC and Na(+) /K(+) -ATPase in vitro in LPS-injured AECII. In-vivo experiments, however, give strong evidence that sevoflurane does not affect water reabsorption and oedema resolution, but possibly oedema formation.


Subject(s)
Acute Lung Injury/pathology , Lung/pathology , Methyl Ethers/pharmacology , Pulmonary Alveoli/metabolism , Pulmonary Edema/pathology , Acute Lung Injury/drug therapy , Anesthetics/pharmacology , Animals , Biological Transport, Active/drug effects , Cell Line , Cell Survival/drug effects , Lipopolysaccharides/pharmacology , Lung/drug effects , Male , Oxygen/analysis , Pulmonary Alveoli/drug effects , Pulmonary Edema/metabolism , Rats , Rats, Wistar , Respiratory Distress Syndrome/metabolism , Rubidium/metabolism , Sevoflurane , Sodium/metabolism , Sodium Channels/drug effects , Sodium Isotopes/metabolism , Sodium-Potassium-Exchanging ATPase/metabolism
8.
J Membr Biol ; 241(3): 145-54, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21584679

ABSTRACT

This study examined the role of cell volume modulation in plasma membrane rupture and death documented in ouabain-treated renal epithelial cells. Long-term exposure to ouabain caused massive death of C11-MDCK (Madin-Darby canine kidney) epithelial cells, documented by their detachment, chromatin cleavage and complete loss of lactate dehydrogenase (LDH), but did not affect the survival of vascular smooth muscle cells (VSMCs) from the rat aorta. Unlike the distinct impact on cell survival, 2-h exposure to ouabain led to sharp elevation of the [Na⁺](i)/[K⁺](i) ratio in both cell types. A similar increment of Na⁺(i) content was evoked by sustained inhibition of Na⁺,K⁺-ATPase in K⁺-free medium. However, in contrast to ouabain, C11-MDCK cells survived perfectly during 24-h exposure to K⁺-free medium. At 3 h, the volume of ouabain-treated C11-MDCK cells and VSMCs, measured by the recently developed dual-image surface reconstruction technique, was increased by 16 and 12%, respectively, whereas 5-10 min before the detachment of ouabain-treated C11-MDCK cells, their volume was augmented by ~30-40%. To examine the role of modest swelling in the plasma membrane rupture of ouabain-treated cells, we compared actions of hypotonic medium on volume and LDH release. We observed that LDH release from hypoosmotically swollen C11-MDCK cells was triggered when their volume was increased by approximately fivefold. Thus, our results showed that the rupture of plasma membranes in ouabain-treated C11-MDCK cells was not directly caused by cell volume modulation evoked by Na⁺,K⁺-ATPase inhibition and inversion of the [Na⁺](i)/[K⁺](i) ratio.


Subject(s)
Cell Membrane/metabolism , Cell Size/drug effects , Epithelial Cells/metabolism , Ion Transport/drug effects , Kidney/metabolism , Muscle, Smooth, Vascular/metabolism , Ouabain/pharmacology , Sodium-Potassium-Exchanging ATPase , Animals , Cell Adhesion/drug effects , Cell Death/drug effects , Cell Line , Cell Survival/drug effects , Chromatin/metabolism , Dogs , Epithelial Cells/cytology , Kidney/cytology , L-Lactate Dehydrogenase/metabolism , Muscle, Smooth, Vascular/cytology , Osmosis/drug effects , Osmotic Pressure/drug effects , Potassium/metabolism , Rats , Rubidium/metabolism , Sodium Isotopes/metabolism , Sodium Radioisotopes/metabolism , Sodium-Potassium-Exchanging ATPase/antagonists & inhibitors , Sodium-Potassium-Exchanging ATPase/metabolism
9.
NMR Biomed ; 23(8): 907-12, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20878968

ABSTRACT

Preterm birth occurs in 12.5% of births in the United States and can lead to risk of infant death or to lifelong serious health complications. A greater understanding by which the two main processes, uterine contraction and cervical remodeling are regulated is required to reduce rates of preterm birth. The cervix must undergo extensive remodeling through pregnancy in preparation for parturition, the process of labor and delivery of young. One key aspect of this dynamic process is a change in the composition and abundance of glycosaminoglycans (GAGs) and proteoglycans within the extracellular matrix, which influences the loss of tensile strength or stiffness of the cervix during labor. 23Na NMR spectroscopy has previously been validated as a method to quantify GAGs in tissues. In the current study, the Na+ concentration was measured at several time points through pregnancy in mouse cervices using 23Na NMR spectroscopy. The Na+ concentration increased progressively during pregnancy and peaked one day before birth followed by a rapid decline after birth. The same trend was seen in GAGs as measured by a biochemical assay using independent cervix samples over the course of pregnancy. We suggest that monitoring the Na+ concentration via 23Na NMR spectroscopy can serve as an informative physiological marker in evaluating the stages of cervical remodeling ex vivo and warrants further investigation to determine its utility as a diagnostic tool for the identification of women at risk for impending preterm birth.


Subject(s)
Biomarkers/analysis , Cervix Uteri , Glycosaminoglycans/analysis , Pregnancy, Animal , Sodium Isotopes/metabolism , Animals , Cervix Uteri/anatomy & histology , Cervix Uteri/chemistry , Cervix Uteri/physiology , Extracellular Matrix/chemistry , Female , Gestational Age , Mice , Mice, Inbred C57BL , Parturition/physiology , Pregnancy , Premature Birth/prevention & control , Proteoglycans/analysis , Water/metabolism
10.
J Magn Reson Imaging ; 30(1): 219-23, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19557742

ABSTRACT

PURPOSE: To demonstrate the use of sodium MRI for measuring the time course of tissue sodium concentration (TSC) in a nonhuman primate model of reversible focal brain ischemia. MATERIALS AND METHODS: Reversible endovascular focal brain ischemia was induced in nonhuman primates (n = 4), and sodium MRI was performed on a 3 Tesla scanner for monitoring changes in TSC during both the middle cerebral artery (MCA) occlusion and MCA reperfusion portions of the experiment. RESULTS: The TSC increased linearly in the ischemic tissue during MCA occlusion (ranging from a mean TSC increase of 5.44%/h to 7.15%/h across the four subjects), and then there was a statistically significant change from a positive TSC slope during MCA occlusion to a TSC slope after MCA reperfusion that was not statistically different from zero. The linear increase in sodium MRI during brain ischemia was used to estimate the stroke onset time to within 0.45 h in each of the four subjects (with a maximum 95% confidence interval of +/- 1.147 h). CONCLUSION: The data indicate that sodium MRI increases linearly during brain ischemia, and that this increase is stopped by tissue reperfusion within 5.4 h after stroke onset.


Subject(s)
Brain Ischemia/metabolism , Magnetic Resonance Imaging/methods , Sodium/metabolism , Animals , Brain Ischemia/pathology , Disease Models, Animal , Haplorhini , Image Processing, Computer-Assisted/methods , Sodium Isotopes/metabolism , Time Factors
11.
J Magn Reson Imaging ; 30(1): 18-24, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19557842

ABSTRACT

PURPOSE: To test the hypotheses that (i) the regional heterogeneity of brain sodium concentration ([Na(+)](br)) provides a parameter for ischemic progression not available from apparent diffusion coefficient (ADC) data, and (ii) [Na(+)](br) increases more in ischemic cortex than in the caudate putamen (CP) with its lesser collateral circulation after middle cerebral artery occlusion in the rat. MATERIALS AND METHODS: (23)Na twisted projection MRI was performed at 3 Tesla. [Na(+)](br) was independently determined by flame photometry. The ischemic core was localized by ADC, by microtubule-associated protein-2 immunohistochemistry, and by changes in surface reflectivity. RESULTS: Within the ischemic core, the ADC ratio relative to the contralateral tissue was homogeneous (0.63 +/- 0.07), whereas the rate of [Na(+)](br) increase (slope) was heterogeneous (P < 0.005): 22 +/- 4%/h in the sites of maximum slope versus 14 +/- 1%/h elsewhere (here 100% is [Na(+)](br) in the contralateral brain). Maximum slopes in the cortex were higher than in CP (P < 0.05). In the ischemic regions, there was no slope/ADC correlation between animals and within the same brain (P > 0.1). Maximum slope was located at the periphery of ischemic core in 8/10 animals. CONCLUSION: Unlike ADC, (23)Na MRI detected within-core ischemic lesion heterogeneity.


Subject(s)
Brain Ischemia/metabolism , Brain/metabolism , Magnetic Resonance Imaging/methods , Sodium/metabolism , Animals , Biomarkers/metabolism , Brain/pathology , Brain Mapping/methods , Disease Models, Animal , Disease Progression , Image Processing, Computer-Assisted/methods , Male , Photometry , Rats , Rats, Sprague-Dawley , Sodium Isotopes/metabolism
12.
Appl Environ Microbiol ; 67(9): 4390-2, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11526055

ABSTRACT

Sodium gradients (DeltapNa) were measured in resting cells of Fibrobacter succinogenes by in vivo 23Na nuclear magnetic resonance using Tm(DOTP)5- [thulium(III) 1,4,7,10-tetraazacyclododecane-N',N",N"'-tetramethylenephosphonate] as the shift reagent. This bacterium was able to maintain a DeltapNa of -55 to -40 mV for extracellular sodium concentrations ranging from 30 to 200 mM. Depletion of Na+ ions during the washing steps led to irreversible damage (modification of glucose metabolism and inability to maintain a sodium gradient).


Subject(s)
Bacteria, Anaerobic/physiology , Rumen/microbiology , Sodium Chloride/metabolism , Animals , Magnetic Resonance Spectroscopy/methods , Organometallic Compounds/metabolism , Organophosphorus Compounds/metabolism , Sodium Isotopes/metabolism
13.
Pflugers Arch ; 436(4): 515-20, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9683723

ABSTRACT

Ca2+ paradox damage has been suggested to be determined by Na+ entry during Ca2+ depletion and exchange of Na+ for Ca2+ during Ca2+ repletion. Since previously a Ca2+ paradox without prior increase of total intracellular [Na+] ([Na+]i) has been observed, we investigated whether local accumulation of Na+ close to the inner side of the sarcolemma during Ca2+ depletion plays a role in the Ca2+ paradox by replacing all extracellular Na+ by Li+ 5 min before and during 10 min Ca2+-free perfusion (37°C) in isolated rat hearts (group I). Subsequently, hearts were perfused with a standard, Na+- and Ca2+-containing solution. Verapamil was used to prevent contracture due to the absence of Na+/Ca2+ exchange during Na+-free perfusion in the presence of Ca2+. In group II, the Ca2+-free period was omitted, and in group III normal extracellular [Na+] was used throughout. 23Na-NMR was used to monitor intra- and extracellular Na+ signals. Total creatine kinase release was 2,977±413, 36±24 and 3170±297 IU/g dry weight in groups I, II and III respectively, indicating a full Ca2+ paradox in groups I and III. [Na+]i decreased from 11.3±0.6 mM during control perfusion to 1.2±0.4 mM after 10 min Ca2+ depletion in group I, whereas in group III [Na+]i was 10.9±2.2 mM during control perfusion and did not change significantly after 10 min Ca2+-free perfusion. It is concluded that accumulation of Na+ close to the inner side of the sarcolemma during Ca2+ depletion is not a prerequisite for the Ca2+ paradox.


Subject(s)
Calcium/metabolism , Myocardium/metabolism , Sodium/metabolism , Animals , Male , Myocardium/cytology , Nuclear Magnetic Resonance, Biomolecular/methods , Rats , Rats, Wistar , Sarcolemma/metabolism , Sodium Isotopes/metabolism
14.
Can J Microbiol ; 21(1): 43-50, 1975 Jan.
Article in English | MEDLINE | ID: mdl-1116038

ABSTRACT

Sodium ion is required for transport of L-alanine and alpha-aminoisobutyric acid (AIB) into cells and membrane vesicles of a marine pseudomonad. Initial rate data obtained at various Naplus and amino acid concentrations were tested for fit by least squares analysis to sequential and Ping-Pong equations. The sequential case is preferred statistically at the 99% confidence limit. Cotransport of AIB and Naplus in cells could not be detected, perhaps explained by efflux of Naplus shown to occur from these cells. The kinetic analysis is consistent with formation of a ternary complex involving Naplus and the amino acid.


Subject(s)
Alanine/metabolism , Aminoisobutyric Acids/metabolism , Pseudomonas/metabolism , Sodium/pharmacology , Water Microbiology , Biological Transport, Active , Carbon Radioisotopes , Cell Fractionation , Cell Membrane/metabolism , Computers , Inulin/metabolism , Kinetics , Oxygen Consumption , Radioisotopes , Seawater , Sodium Isotopes/metabolism , Stereoisomerism , Sucrose/metabolism , Tritium
17.
J Clin Invest ; 52(5): 975-90, 1973 May.
Article in English | MEDLINE | ID: mdl-4573355

ABSTRACT

In this study we use the multiple indicator dilution technique to outline the kinetic mechanisms underlying the uptake of rubidium, a cation which, in the steady state, is concentrated by hepatic parenchymal cells. We inject a mixture of (51)Cr-labeled red blood cells (a vascular reference substance), (22)Na (which is confined to the extracellular space, the expected extravascular distribution space for rubidium, in the absence of cellular uptake), and (86)Rb into the portal vein and obtain normalized outflow patterns, expressed as outflowing fractions of each injected mass per milliliter vs. time. The labeled red cell curve rises to the highest and earliest peak and decays rapidly. That for labeled sodium rises to a later and lower peak, and decays less rapidly. Its extrapolated recovery is equal to that for the red cells. The observed (86)Rb curve consists of two parts: an early clearly defined peak of reduced area, related to the (22)Na peak in timing; and a later tailing, obscured by recirculation, so that total outflow recovery cannot be defined (even though it would be expected to be the same). We model the concentrative uptake of (86)Rb and find two corresponding outflow fractions: throughput material, which sweeps past the cell surface as a wave delayed with respect to the vascular reference (tracer which has not entered cells); and exchanging material (tracer which has entered cells and later returns to the circulation). We find that the outflow form of the rubidium curve, the presence of both a relatively clearly defined throughput component and a relatively prolonged low-in-magnitude tailing, is consequent to the concentrative character of the transport mechanism, to the presence of an influx rate constant many times the efflux rate constant. The modeling which we develop is general, and has potential application in situations where transport is nonconcentrative.


Subject(s)
Liver/metabolism , Rubidium/metabolism , Animals , Biological Transport, Active , Cell Membrane Permeability , Chromium Isotopes , Dogs , Erythrocytes/metabolism , Extracellular Space , Liver/blood supply , Liver Circulation , Microcirculation , Models, Biological , Portal Vein , Radioisotope Dilution Technique , Sodium Isotopes/metabolism , Time Factors
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