ABSTRACT
BACKGROUND: Candida is a fungus that causes various types of candidemia, which is the fourth major infectious disease of the blood system. MALDI-TOF-MS is a simple and rapid detection instrument. The aim of the present study was to verify the accuracy of MALDI-TOF-MS in detecting Candida. METHOD: A pooled analysis of articles on MALDI-TOF-MS for diagnosis of candidemia was performed. The quality of original research was assessed using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) guidelines. Stata 12.0 software was used to merge the correct identification rates of Candida and Candida subspecies and obtain pooled sensitivity and specificity of the diagnostic methods. Heterogeneity was found in the subgroup analysis of the included articles. Hence, we explored the factors causing the heterogeneity and its impact on the overall situation. Sensitivity analysis was used to examine the effect of Candida level on total response. Egger's test was used to evaluate the publication bias of the included articles. RESULTS: A total of 16 articles in Pubmed, 79 articles in Embase, 1 article in Cochrane Library, 30 articles in Web of Science and 3 from other sources were identified, of which 10 articles were included based on the inclusion and exclusion criteria. The overall identification accuracy was 100%. CONCLUSION: The accuracy of MALDI-TOF-MS for the identification of Candida was 100%. Further research is necessary to determine whether MALDI-TOF-MS can be used as a clinical diagnostic standard for the identification of Candida.
Subject(s)
Candida/metabolism , Candidiasis/diagnosis , Candidiasis/metabolism , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/classification , Candida/classification , HumansABSTRACT
La espectrometría de masas MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) se ha establecido en los últimos años como una herramienta diagnóstica de primera línea en la identificación de microorganismos, incluyendo los que producen infecciones en el ser humano. La detección rápida de resistencias antimicrobianas es una de las aplicaciones futuras con más expectativas de éxito de esta técnica. En esta revisión se va a tratar de hacer una aproximación a los trabajos más relevantes que se han publicado en este campo, discutiendo los potenciales retos futuros y su aplicación clínica en los próximos años
In recent years, MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry has become established as a first-line diagnostic tool in the identification of microorganisms, including those producing human infections. Rapid detection of antimicrobial resistance is one of the future applications of this technique with the greatest likelihood of success. This review describes the most important studies published in this field and discusses potential future challenges and the clinical application of this technique in the next few years
Subject(s)
Humans , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/classification , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Drug Resistance, Bacterial , Drug Resistance, Multiple, Bacterial , Bacterial Infections/diagnosis , Bacterial Infections/microbiology , Bacterial Infections/classification , Computational Biology/methods , Bacterial Infections/drug therapyABSTRACT
La espectrometría de masas MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) se ha convertido rápidamente en una herramienta de indiscutible utilidad para la identificación y caracterización de microorganismos en numerosos laboratorios de microbiología clínica. La técnica es rápida, fiable y económica y ofrece múltiples posibilidades en distintas áreas de la microbiología. En esta revisión se ofrece una visión general de su utilidad para los estudios de tipificación y epidemiología bacteriana detallando las aproximaciones metodológicas que pueden emplearse tanto para su realización como para el análisis de resultados que se obtienen. Por último, se resumen los estudios existentes sobre su uso en la caracterización de distintas especies bacterianas
MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry has emerged as a potential tool for microbial characterization and identification in many microbiology departments. The technology is rapid, sensitive, and relatively inexpensive in terms of both the labour and costs involved. This review provides an overview on its utility for strain typing and epidemiological studies and explains the methodological approaches that can be used both for the performance of the technique and for the analysis of results. Finally, the review summarizes studies on the characterization of distinct bacterial species
