Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 99
Filter
1.
Histochem Cell Biol ; 156(6): 523-526, 2021 Dec.
Article in English | MEDLINE | ID: mdl-34799748

ABSTRACT

One hundred and twenty years ago, the Hungarian physician Julius von Kossa developed a now classical staining method for detecting mineral deposits in animal tissues. Since then, this method has been widely adapted and combined with different counterstains, but still bears the name of its original inventor, who, if alive, would have turned 150 in 2015. As a rather inexpensive technique that does not require special instrumentation, von Kossa's staining method became extremely popular for demonstrating mineralized tissues in vivo and in vitro. This article pays tribute to von Kossa and to his handy staining method.


Subject(s)
Staining and Labeling/history , Animals , History, 19th Century , History, 20th Century , Humans
3.
Am J Dermatopathol ; 42(10): 731-738, 2020 Oct.
Article in English | MEDLINE | ID: mdl-32675471

ABSTRACT

Joseph von Gerlach was an eminent German anatomist and pioneer of histology. He devised various techniques to assess the fine structure of tissues, most notably a procedure of staining histologic sections that marked the beginning of routine staining in histology. Gerlach was also one of the pioneers of microphotography.


Subject(s)
Histological Techniques/history , Histology/history , Photography/history , Anatomy/history , Coloring Agents , Germany , Histological Techniques/methods , History, 19th Century , Photography/methods , Staining and Labeling/history
4.
Anat Histol Embryol ; 48(6): 552-556, 2019 Nov.
Article in English | MEDLINE | ID: mdl-31679158

ABSTRACT

In the early days of plastination, plastinate Color was the usual grey/brown familiar to formalin-fixed biological specimens. Initially, trials with Kaiserling's, Klotz, Jore's and McCormick's fixative solutions were disappointing. Vascular injections with Colored epoxy were a great breakthrough in the 1980s. Biodur AC10® stain was the first stain of note to be applied to gross specimens to be plastinated and was applied in the last acetone bath. As plastination became more popular, specimen Color became an important and necessary aspect. Reactivation of the normal Color of red blood cells within a formalin-fixed specimen was introduced as a mechanism to restore Color to plastinated specimens. Painting of plastinated vessels was tried with some success, and finally, a superior new proprietary type of silicone coloration was developed. More recently, a versatile red pigment stain was developed. All of these have added aesthetically to the plastination processes and will certainly be a reality in the years to come. The various methodologies to Color plastinates are presented. Time will tell how effective these may or may not be.


Subject(s)
Coloring Agents/history , Plastination/methods , Animals , Coloring Agents/chemistry , History, 20th Century , History, 21st Century , Humans , Models, Anatomic , Silicones , Staining and Labeling/history , Staining and Labeling/methods
5.
Int J Surg Pathol ; 27(1): 4-14, 2019 Feb.
Article in English | MEDLINE | ID: mdl-30001639

ABSTRACT

Hematoxylin is a basic dye derived from the heartwood of Palo de Campeche ( Haematoxylum campechianum), the logwood tree native to Mexico and Central America. Haematoxylum means "bloodwood" in reference to its dark-red heartwood and campechianum refers to its site of origin, the coastal city of Campeche on the Yucatan Peninsula, Mexico. Hematoxylin is colorless but it turns into the color dye hematein after oxidation (ripening). The dyeing property of logwood was well-known to the natives of the Yucatan Peninsula before the arrival of the Spaniards who brought it to Europe shortly after the discovery of the Americas. An important trade soon developed related to growing and preparing hematoxylin for dyeing fabrics. Pirates discovered that one shipload of logwood was equivalent to a year's value from any other cargo, and by 1563, more than 400 pirate vessels wandered the Atlantic Ocean and attacked Spanish galleons transporting gold, silver, and logwood from the Americas to Europe. Hematoxylin and eosin is a staining method that dates back to the late 19th century. In 1865 and 1891, Böhmer and Meyer, respectively, first used hematoxylin in combination with a mordant (alum). Later, with the use of anilines by Ehrlich, the repertoire of stains expanded rapidly resulting in the microscopic descriptions of multiple diseases that were defined by their stainable features. Today hematoxylin, along with eosin, remains the most popular stain in histology.


Subject(s)
Hematoxylin/history , Staining and Labeling/history , History, 15th Century , History, 16th Century , History, 17th Century , History, 18th Century , History, 19th Century , History, 20th Century , History, 21st Century , North America , Trees
6.
Biosci Rep ; 39(1)2019 01 31.
Article in English | MEDLINE | ID: mdl-30567726

ABSTRACT

Staining with Congo Red (CR) is a qualitative method used for the identification of amyloids in vitro and in tissue sections. However, the drawbacks and artefacts obtained when using this dye can be found both in vitro and in vivo Analysis of scientific data from previous studies shows that CR staining alone is not sufficient for confirmation of the amyloid nature of protein aggregates in vitro or for diagnosis of amyloidosis in tissue sections. In the present paper, we describe the characteristics and limitations of other methods used for amyloid studies. Our historical review on the use of CR staining for amyloid studies may provide insight into the pitfalls and caveats related to this technique for researchers considering using this dye.


Subject(s)
Amyloid/analysis , Amyloidosis/diagnosis , Coloring Agents/chemistry , Congo Red/chemistry , Staining and Labeling/methods , Amyloid/history , Amyloidosis/history , Amyloidosis/pathology , Benzothiazoles/chemistry , Benzothiazoles/history , Coloring Agents/history , Congo Red/history , History, 17th Century , History, 18th Century , History, 19th Century , History, 20th Century , History, 21st Century , Humans , Immunohistochemistry/history , Immunohistochemistry/methods , Protein Aggregates , Staining and Labeling/history
8.
Semin Diagn Pathol ; 35(6): 354-359, 2018 Nov.
Article in English | MEDLINE | ID: mdl-30366791

ABSTRACT

Histochemistry has a history which, in some ways, goes back to ancient times. The desire for humans to understand the workings of their bodies, and the roles that various chemicals have in them, is long-standing. This review considers the evolution of histochemistry and cytochemistry as scientific disciplines, culminating in the pairing of those techniques with basic biochemistry. They have served as the bases for a synthesis of microscopy, chemistry, immunology, and molecular biology, particularly in the practice of anatomic pathology.


Subject(s)
Histocytochemistry/history , Pathology/history , Staining and Labeling/history , Biopsy/history , Diffusion of Innovation , History, 19th Century , History, 20th Century , History, 21st Century , Humans , Predictive Value of Tests , Reproducibility of Results
9.
Int Arch Allergy Immunol ; 176(1): 55-60, 2018.
Article in English | MEDLINE | ID: mdl-29597213

ABSTRACT

The specificity of several staining methods for mast cells provides the pathologist with a useful means for the differential diagnosis of mast cell tumors. Mast cells stain metachromatically with toluidine blue with greater intensity in cells containing smaller granules. Most stains for mast cells rely on the cell's content of heparin, other glycosaminoglycans, and esterase. As an alternative to histochemical stains, different antibodies have been used to identify mast cells in humans.


Subject(s)
Mast Cells , Staining and Labeling/history , Animals , Biomarkers/metabolism , Europe , History, 19th Century , History, 20th Century , Humans , Mast Cells/immunology , Mast Cells/metabolism , Mast Cells/pathology , Rats , Staining and Labeling/methods , United States
12.
Microbiol Spectr ; 5(2)2017 03.
Article in English | MEDLINE | ID: mdl-28337966

ABSTRACT

Acid-fast (AF) staining, also known as Ziehl-Neelsen stain microscopic detection, developed over a century ago, is even today the most widely used diagnostic method for tuberculosis. Herein we present a short historical review of the evolution of AF staining methods and discuss Koch's paradox, in which non-AF tubercle bacilli can be detected in tuberculosis patients or in experimentally infected animals. The conversion of Mycobacterium tuberculosis from an actively growing, AF-positive form to a nonreplicating, AF-negative form during the course of infection is now well documented. The mechanisms of loss of acid-fastness are not fully understood but involve important metabolic processes, such as the accumulation of triacylglycerol-containing intracellular inclusions and changes in the composition and spatial architecture of the cell wall. Although the precise component(s) responsible for the AF staining method remains largely unknown, analysis of a series of genetically defined M. tuberculosis mutants, which are attenuated in mice, pointed to the primary role of mycolic acids and other cell wall-associated (glyco)lipids as molecular markers responsible for the AF property of mycobacteria. Further studies are now required to better describe the cell wall reorganization that occurs during dormancy and to develop new staining procedures that are not affected by such cell wall alterations and that are capable of detecting AF-negative cells.


Subject(s)
Bacteriological Techniques/methods , Mycobacterium tuberculosis/cytology , Mycobacterium tuberculosis/growth & development , Staining and Labeling/methods , Tuberculosis/microbiology , Animals , Bacteriological Techniques/history , History, 19th Century , History, 20th Century , History, 21st Century , Humans , Mycobacterium tuberculosis/metabolism , Mycolic Acids/metabolism , Staining and Labeling/history
13.
Biotech Histochem ; 92(1): 29-35, 2017.
Article in English | MEDLINE | ID: mdl-28098484

ABSTRACT

I give an historical account and analysis of the scientific priority of the discovery of the polychrome staining of microscopic biological preparations provided by mixtures of eosin plus methylene blue and its derivatives, especially azure B. I maintain that both the formal priority for the discovery of the polychrome staining phenomenon and credit for initiating the development of a technique of polychrome staining properly belong to D. L. Romanowsky. His scientific work demonstrated the possibility of using a simple technique to stain hematological preparations selectively to give good contrast, high resolution and the ability to identify malaria parasites. Romanowsky's approach constituted the starting point for the development of a family of polychrome stains for microscopic investigation of hematological preparations by a number of his contemporaries.


Subject(s)
Azure Stains/history , Eosine Yellowish-(YS)/chemistry , Methylene Blue/chemistry , Staining and Labeling/history , Staining and Labeling/methods , Coloring Agents/chemistry , Eosine Yellowish-(YS)/history , History, 19th Century , Humans , Malaria/diagnosis , Malaria/parasitology , Plasmodium/cytology
16.
J Histochem Cytochem ; 63(8): 543-58, 2015 Aug.
Article in English | MEDLINE | ID: mdl-26216133

ABSTRACT

Before the middle of the previous century, cell types of the pancreatic islets of Langerhans were identified primarily on the basis of their color reactions with histological dyes. At that time, the chemical basis for the staining properties of islet cells in relation to the identity, chemistry and structure of their hormones was not fully understood. Nevertheless, the definitive islet cell types that secrete glucagon, insulin, and somatostatin (A, B, and D cells, respectively) could reliably be differentiated from each other with staining protocols that involved variations of one or more tinctorial techniques, such as the Mallory-Heidenhain azan trichrome, chromium hematoxylin and phloxine, aldehyde fuchsin, and silver impregnation methods, which were popularly used until supplanted by immunohistochemical techniques. Before antibody-based staining methods, the most bona fide histochemical techniques for the identification of islet B cells were based on the detection of sulfhydryl and disulfide groups of insulin. The application of the classical islet tinctorial staining methods for pathophysiological studies and physiological experiments was fundamental to our understanding of islet architecture and the physiological roles of A and B cells in glucose regulation and diabetes.


Subject(s)
Islets of Langerhans/cytology , Langerhans Cells/cytology , Staining and Labeling/history , Diabetes Mellitus/history , Diabetes Mellitus/metabolism , Glucose/history , Glucose/metabolism , Histocytochemistry/history , History, 19th Century , History, 20th Century , History, 21st Century , Homeostasis , Humans , Islets of Langerhans/metabolism , Langerhans Cells/metabolism
18.
Methods Mol Biol ; 1266: 1-6, 2015.
Article in English | MEDLINE | ID: mdl-25560064

ABSTRACT

The biarsenical-tetracysteine tagging system was the first of (and inspiration for) the now numerous methods for site-specifically labeling proteins in living cells with small molecules such as fluorophores. This historical recollection describes its conception and the trial-and-error chemical development required to become a versatile technique.


Subject(s)
Arsenicals/chemistry , Cysteine/chemistry , Recombinant Fusion Proteins/chemistry , History, 20th Century , Staining and Labeling/history
SELECTION OF CITATIONS
SEARCH DETAIL
...