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1.
Int J Syst Evol Microbiol ; 70(8): 4637-4645, 2020 Aug.
Article in English | MEDLINE | ID: mdl-32672529

ABSTRACT

Six Staphylococcus strains were isolated from healthy black bears (Ursus americanus) in the Great Smoky Mountains National Park, Tennessee, USA. Phylogenetic analysis based on complete genome, 16S rRNA, dnaJ, hsp60, rpoB and sodA genes, and MALDI-TOF-MS main spectral profiles revealed that the strains belonged to one species and showed the closest relatedness to members of the 'Staphylococcus intermedius group' (SIG), which include Staphylococcus intermedius, Staphylococcus pseudintermedius, Staphylococcus delphini and Staphyloccoccus cornubiensis. The strains were positive in SIG-specific and negative in individual species-specific PCR assays for the nuc gene. The strains can be differentiated from the other SIG species by the absence of sucrose fermentation, from S. intermedius DSM 20373T, S. pseudintermedius CCUG 49543T and S. cornubiensis DSM 105366T by the absence of methyl ß-d-glucopyranoside fermentation and from S. delphini DSM 20771T by fermentation of trehalose. DNA relatedness of the type strain MI 10-1553T with the type strains of S. delphini, S. pseudintermedius, S. intermedius and S. cornubiensis was ≤48.2 % by digital DNA-DNA hybridization and ≤92.3 % by average nucleotide identity calculations. Iso-C15:0, anteiso-C15 : 0 and anteiso-C17 : 0 were the most common fatty acids. Polar lipids consisted of phosphadidylglycerols, phospholipids, glycolipid, diphosphatidylglycerol and aminophospholipid. Cell-wall peptidoglycan was of type A3α l-Lys-Gly3 (Ser; similar to A11.2 and A11.3). The respiratory quinone belonged to menaquinone 7 (MK-7). The G+C content of MI 10-1553T was 39.3 mol%. The isolated strains represent a novel species of the genus Staphylococcus, for which we propose the name Staphylococcus ursi sp. nov. The type strain is MI 10-1553T (=ATCC TSD-55T=CCOS 1900T).


Subject(s)
Phylogeny , Staphylococcus/classification , Ursidae/microbiology , Animals , Bacterial Typing Techniques , Base Composition , DNA, Bacterial/genetics , Fatty Acids/chemistry , Genes, Bacterial , Nucleic Acid Hybridization , Peptidoglycan/chemistry , Phospholipids/chemistry , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Staphylococcus/isolation & purification , Staphylococcus intermedius/genetics , Tennessee , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistry
2.
Folia Microbiol (Praha) ; 64(2): 231-236, 2019 Mar.
Article in English | MEDLINE | ID: mdl-30238302

ABSTRACT

A group of 59 putative strains of Staphylococcus intermedius/Staphylococcus pseudintermedius deposited in the Czech National Collection of Type Cultures (CNCTC, National Institute for Public Health, Prague, Czech Republic) and the National Reference Laboratory for Staphylococci (NRL for Staphylococci, National Institute for Public Health, Prague, Czech Republic) was reclassified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). There the biggest human collection of S. pseudintermedius in Europe was analysed; 44 samples (75%) were of human origin. Twenty-two percent (n = 13) of the strains were isolated from animals, and two staphylococci were of unknown origin. This study revealed the prevalence of Staphylococcus pseudintermedius (94%, n = 53) vs. Staphylococcus intermedius (6%, n = 6) in the collection of human and veterinary staphylococci after reclassification. Results of PCR-RFLP analysis were verified by comparison with a repetitive element sequence-based polymerase chain reaction (Rep-PCR) analysis on 26 (44%) randomly selected strains. Due to a low-resolution ability of PCR-RFLP to separate Staphylococcus intermedius from Staphylococcus delphini, four isolates of Staphylococcus intermedius were biochemically verified further to exclude the presence of Staphylococcus delphini in the collection. Our results indicate that S. intermedius and S. pseudintermedius have occurred independently over an age-long period of their co-evolution.


Subject(s)
Biological Evolution , Staphylococcal Infections/microbiology , Staphylococcal Infections/veterinary , Staphylococcus/classification , Animals , Bacterial Proteins/genetics , Bacteriological Techniques , DNA, Bacterial/genetics , Humans , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Repetitive Sequences, Nucleic Acid/genetics , Sequence Analysis, DNA , Staphylococcus/genetics , Staphylococcus/isolation & purification , Staphylococcus intermedius/classification , Staphylococcus intermedius/genetics , Staphylococcus intermedius/isolation & purification
3.
J Comp Pathol ; 156(2-3): 152-157, 2017.
Article in English | MEDLINE | ID: mdl-28041618

ABSTRACT

A 40-day-old male, blue heeler puppy with hindlimb ataxia, nystagmus, apathy, motor incoordination and hyperaesthesia of the forelimbs died 3 days after the onset of clinical signs. Significant gross findings included cerebellar herniation, cerebral oedema and dilation of the third and right lateral cerebral ventricles due to the accumulation of a purulent exudate. Histopathological examination revealed pyogenic ventriculitis and purulent meningoencephalitis. Pure colonies of a coagulase-positive Staphylococcus were isolated from the purulent cerebral exudate. A polymerase chain reaction assay that targeted the 16S rRNA gene of bacteria amplified the desired product from bacterial colonies. Direct sequencing revealed the organism to be Staphylococcus pseudintermedius. Phylogenetic analyses showed that the organism was antigenically similar to Staphylococcus intermedius and Staphylococcus delphini, being part of the S. intermedius group of bacteria. These findings confirmed the participation of S. pseudintermedius in the development of the pathological manifestations and lesions observed in this puppy.


Subject(s)
Cerebral Ventriculitis/veterinary , Empyema/veterinary , Staphylococcal Infections/veterinary , Animals , Dogs , Male , Phylogeny , RNA, Ribosomal, 16S , Staphylococcus intermedius/genetics
4.
Vet Surg ; 45(4): 499-506, 2016 May.
Article in English | MEDLINE | ID: mdl-27079435

ABSTRACT

OBJECTIVE: To evaluate the expression of biofilm-associated genes in Staphylococcus pseudintermedius on multiple clinically relevant surfaces. STUDY DESIGN: In vitro experimental study. SAMPLE POPULATION: Two strains of methicillin-resistant S. pseudintermedius isolated from clinical infections representing the most common international isolates. METHODS: A quantitative polymerase chain reaction (qPCR) assay for expression of genes related to biofilm initial adhesion, formation/maturation, antimicrobial resistance, and intracellular communication was developed and validated. S. pseudintermedius biofilms were grown on 8 clinically relevant surfaces (polymethylmethacrylate, stainless steel, titanium, latex, silicone, polydioxanone, polystyrene, and glass) and samples of logarithmic and stationary growth phases were collected. Gene expression in samples was measured by qPCR. RESULTS: Significant differences in gene expression were identified between surfaces and between bacterial strains for most gene/strain/surface combinations studied. Expression of genes responsible for production of extracellular matrix were increased in biofilms. Expression of genes responsible for initial adhesion and intracellular communication was markedly variable. Antimicrobial resistance gene expression was increased on multiple surfaces, including stainless steel and titanium. CONCLUSION: A method for evaluation of expression of multiple biofilm-associated genes in S. pseudintermedius was successfully developed and applied to the study of biofilms on multiple surfaces. Variations in expression of these genes have a bearing on understanding the development and treatment of implant-associated biofilm infections and will inform future clinical research.


Subject(s)
Methicillin Resistance , Prostheses and Implants/microbiology , Staphylococcal Infections/veterinary , Staphylococcus intermedius/isolation & purification , Animals , Biofilms , Gene Expression Regulation, Bacterial , Polymerase Chain Reaction/veterinary , Polymethyl Methacrylate , Stainless Steel , Staphylococcal Infections/microbiology , Staphylococcus intermedius/genetics
5.
Pol J Vet Sci ; 19(1): 7-14, 2016.
Article in English | MEDLINE | ID: mdl-27096782

ABSTRACT

The aim of this study was to characterize Staphylococcus pseudintermedius for its antimicrobial resistance and virulence factors with a special focus on methicillin-resistant (MRSP) strains isolated from sick dogs in Lithuania. Clinically sick adult dogs suffering from infections (n=214) and bitches with reproductive disorders (n=36) from kennels were selected for the study. Samples (n=192) from the 250 tested (76.8%) dogs were positive for Staphylococcus spp. Molecular profiling using the species-specific nuc gene identified 51 isolates as S. pseudintermedius (26.6% from a total number of isolated staphylococci) of which 15 isolates were identified as MRSP. Ten MRSP isolates were isolated from bitches with reproductive disorders from two large breeding kennels. Data on susceptibility of S. pseudintermedius to different antimicrobials revealed that all isolates were susceptible to vancomycin, daptomycin and linezolid. Two isolates (3.9%) were resistant to rifampicin. A high resistance was seen towards penicillin G (94.1%), tetracycline (64.7%) and macrolides (68.7%). Resistance to fluoroquinolones ranged from 25.5% (gatifloxacin) to 31.4% (ciprofloxacin). The most prevalent genes encoding resistance included blaZ, aac(6')-Ie-aph(2'')-Ia, mecA, and tet(M). The Luk-I gene encoding a leukotoxin was detected in 29% of the isolates, whereas the siet gene encoding exfoliative toxin was detected in 69% of the S. pseudintermedius isolates. This report of MRSP in companion animals represents a major challenge for veterinarians in terms of antibiotic therapy and is a concern for both animal and public health.


Subject(s)
Anti-Bacterial Agents/pharmacology , Dog Diseases/microbiology , Staphylococcal Skin Infections/veterinary , Staphylococcus intermedius/drug effects , Animals , Dog Diseases/epidemiology , Dogs , Drug Resistance, Multiple, Bacterial , Gene Expression Regulation, Bacterial/physiology , Lithuania/epidemiology , Staphylococcal Skin Infections/epidemiology , Staphylococcal Skin Infections/microbiology , Staphylococcus intermedius/genetics , Staphylococcus intermedius/metabolism , Virulence Factors/genetics , Virulence Factors/metabolism
6.
Vet Dermatol ; 26(5): 334-8, e70-1, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26216025

ABSTRACT

BACKGROUND: Using the US Clinical and Laboratory Standards Institute (CLSI) human tetracycline breakpoints to predict minocycline and doxycycline susceptibility of Staphylococcus pseudintermedius (SP) isolates from dogs is not appropriate because they are too high to meet pharmacokinetic/pharmacodynamic data using a standard dose. New breakpoints have been approved for doxycycline and proposed for minocycline. Revised breakpoints are four dilutions lower than tetracycline breakpoints, providing a more conservative standard for classification of isolates. HYPOTHESIS/OBJECTIVES: The objectives of this study were to measure minimum inhibitory concentrations (MICs) of minocycline and doxycycline of 100 canine meticillin-resistant SP clinical isolates, compare their susceptibilities to minocycline and doxycycline based on current and revised standards, and document their tetracycline resistance genes. METHODS: E-test strips were used to determine MICs. PCR was used to identify tet genes. RESULTS: Using the human tetracycline breakpoint of MIC ≤ 4 µg/mL, 76 isolates were susceptible to minocycline and 36 isolates were susceptible to doxycycline. In contrast, using the proposed minocycline breakpoint (MIC ≤ 0.25 µg/mL) and approved doxycycline breakpoint (MIC ≤ 0.125 µg/mL), 31 isolates were susceptible to both minocycline and doxycycline. Thirty-one isolates carried no tet genes, two had tet(K) and 67 had tet(M). CONCLUSIONS AND CLINICAL IMPORTANCE: Use of the human tetracycline breakpoints misclassified 45 and five of the isolates as susceptible to minocycline and doxycycline, respectively. PCR analysis revealed that 43 and five of the isolates classified as susceptible to minocycline and doxycycline, respectively, possessed the tetracycline resistance gene, tet(M), known to confer resistance to both drugs. These results underscore the importance of utilizing the proposed minocycline and approved doxycycline canine breakpoints in place of human tetracycline breakpoints.


Subject(s)
Anti-Bacterial Agents/therapeutic use , Dog Diseases/drug therapy , Doxycycline/therapeutic use , Methicillin-Resistant Staphylococcus aureus/drug effects , Minocycline/therapeutic use , Staphylococcal Skin Infections/veterinary , Staphylococcus intermedius/drug effects , Animals , Dog Diseases/microbiology , Dogs/microbiology , Methicillin Resistance , Microbial Sensitivity Tests/veterinary , Staphylococcal Skin Infections/drug therapy , Staphylococcal Skin Infections/microbiology , Staphylococcus intermedius/genetics
7.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 33(6): 391-396, jun.-jul. 2015. tab
Article in English | IBECS | ID: ibc-142121

ABSTRACT

INTRODUCTION: Staphylococcus aureus and Staphylococcus pseudintermedius are highly important due to their capacity for producing diseases in humans and animals, respectively. The aim of the study was to investigate and characterize the coagulase positive Staphylococcus (CoPS) carriage in a Primary Healthcare Center population. METHODS: Nasal swabs were obtained from 281 non-infectious patients. The CoPS isolates recovered were typed, and their resistance phenotype and genotype, as well as their virulence profiles, were analyzed. RESULTS: CoPS isolates were recovered from 56/281 patients (19.9%). Fifty-five were S. aureus (19.6%), 54 were methicillin susceptible (MSSA) and one was methicillin resistant (MRSA). The remaining isolate was S. pseudintermedius (0.4%). A high diversity of spa-types (n = 40) was detected, with 6 of them being new ones. The multi-locus-sequence-typing of 13 MSSA and one MRSA selected isolates was performed and the STs detected were: ST8, ST15, ST30, ST34, ST121, ST146, ST398, ST554, ST942, ST2499, and ST2500 (the last two STs being new). One MSSA isolate was typed as t1197-ST398-(Clonal complex)CC398. The MRSA isolate was typed as t002-ST146-CC5-SCCmec-IVc, and exhibited a multiresistance phenotype. The detected resistances were: penicillin (76%), macrolides (7%), tetracycline (7%), trimethoprim-sulfamethoxazole (7%), quinolones (7%), and lincosamides (5%). Five isolates contained lukF/lukS-PV genes, 17 tst gene, one eta gene, and two etb gene. The S. pseudintermedius isolate presented a new spa-type (t57) (belonging to a new ST180) and the geneslukS/F-I, siet, se-int, and expB. CONCLUSIONS: A high genetic diversity of S. aureus was detected. Mention must be made of the identification of MSSA CC398 and S. pseudintermedius isolates in two patients, one of them with animal contact. The detection of the genes lukF/lukS-PV and tst should be noted


INTRODUCCIÓN: Staphylococcus aureus y Staphylococcus pseudintermedius son 2 especies de gran importancia que pueden producir enfermedades tanto en personas como en animales. El objetivo del trabajo fue estudiar el estado de portador nasal de aislados de Staphylococcus coagulasa positiva (SCoP) en pacientes de un centro de atención primaria. MÉTODOS: Se analizaron muestras nasales de 281 pacientes sin patología infecciosa. Se tiparon los aislados SCoP y se estudiaron sus fenotipos y genotipos de resistencia y sus perfiles de virulencia. RESULTADOS: Se aislaron SCoP en 56/281 pacientes (19,9%): 55 de los aislados fueron S. aureus (19,6%), 54 sensibles a la meticilina (SASM) y uno resistente a la meticilina (SARM). El aislado restante correspondió a S. pseudintermedius (0,4%). Se detectó una alta diversidad de tipos de spa (n = 40), identificándose 6 nuevos tipos. Se realizó el multi-locus-sequence-typing de 13 cepas SASM y una cepa SARM seleccionadas y se detectaron los siguientes STs: ST8, ST15, ST30, ST34, ST121, ST146, ST398, ST554, ST942, ST2499 y ST2500 (los 2 últimos nuevos). Una de las cepas SASM se tipó como t1197-ST398-(Clonal Complex)CC398. La cepa SARM se tipó como t002-ST146-CC5-SCCmec-IVc y mostró un fenotipo de multirresistencia. Se detectó resistencia a: penicilina (76%), macrólidos (7%), tetraciclina (7%), trimetoprim-sulfametoxazol (7%), quinolonas (7%) y lincosamidas (5%). Se identificaron los genes (número de cepas): lukF/lukS-PV (5), tst (17), eta (1) y etb (2). La cepa de S. pseudintermedius presentó un spa nuevo (t057), una secuencia tipo nueva (ST180), y contenía los genes lukS/F-I, siet, se-int y expB. CONCLUSIONES: Se detectó una alta diversidad genética entre los aislados de SASM. Destaca la identificación de una cepa SASM CC398 (en un veterinario) y otra de S. pseudintermedius, y la frecuente detección de los genes lukF/lukS-PV, tst, eta o etb entre las cepas SASM


Subject(s)
Female , Humans , Male , Staphylococcus aureus/isolation & purification , Staphylococcus aureus/genetics , Staphylococcus aureus/pathogenicity , Methicillin-Resistant Staphylococcus aureus/isolation & purification , Methicillin-Resistant Staphylococcus aureus/genetics , Methicillin-Resistant Staphylococcus aureus/pathogenicity , Staphylococcus intermedius/isolation & purification , Staphylococcus intermedius/pathogenicity , Staphylococcus intermedius/genetics , Epidemiological Monitoring/trends , Primary Health Care , Health Centers , Nasal Mucosa/microbiology , Carrier State , Molecular Typing , Drug Resistance, Microbial , Spain/epidemiology
8.
J Vet Diagn Invest ; 27(2): 231-5, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25680922

ABSTRACT

It has been proposed, based on taxonomic and molecular studies, that all canine isolates belonging to Staphylococcus intermedius group (SIG) should be renamed Staphylococcus pseudintermedius. However, isolates of SIG and other coagulase-positive staphylococci share many phenotypic characteristics, which could lead to misidentification. The accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identifying S. pseudintermedius isolates obtained from canine infections was evaluated, using a polymerase chain reaction (PCR)-based identification as the gold standard. In addition, MALDI-TOF MS was compared with conventional biochemical tests. A central problem was the incorrect identification of S. pseudintermedius isolates as S. intermedius by either MALDI-TOF MS or biochemical identification. From the 49 S. pseudintermedius isolates identified by the molecular method, only 21 could be assigned to this species by the biochemical approach and only 12 by MALDI-TOF MS. The 6 S. aureus isolates were correctly identified by all 3 techniques. However, using biochemical tests, 9 S. pseudintermedius were mistakenly classified as S. aureus, indicating a reduced specificity relative to the MALDI-TOF MS system. Analysis with the MALDI-TOF MS platform allowed rapid and accurate identification of the 49 isolates to the S. intermedius group but the approach was very limited in identifying S. pseudintermedius isolates, as only 12 of 49 isolates were correctly identified, a sensitivity of 0.24 (95% confidence interval: 0.13-0.39).


Subject(s)
Dog Diseases/diagnosis , Staphylococcal Infections/veterinary , Staphylococcus intermedius/isolation & purification , Animals , DNA, Bacterial/analysis , Dog Diseases/microbiology , Dogs , Polymerase Chain Reaction/veterinary , Sensitivity and Specificity , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/veterinary , Staphylococcal Infections/diagnosis , Staphylococcus intermedius/genetics
9.
Braz J Microbiol ; 45(1): 235-7, 2014.
Article in English | MEDLINE | ID: mdl-24948938

ABSTRACT

The study evaluated cefoxitin disk diffusion tests breakpoints and their correlation to mecA gene PCR results for detecting Methicillin-resistant Staphylococcus intermedius Group (MRSP) isolates from dogs in Brazil. Agreement using proposed breakpoint (resistant ≤ 30 mm) was encouraging. The current study reinforces that an epidemiological breakpoint can be established to predict presence of MRSP.


Subject(s)
Anti-Bacterial Agents/pharmacology , Cefoxitin/pharmacology , Genes, Bacterial , Methicillin Resistance , Polymerase Chain Reaction/methods , Staphylococcus intermedius/drug effects , Staphylococcus intermedius/genetics , Animals , Brazil , Dogs , Microbial Sensitivity Tests/methods
10.
Vet Microbiol ; 159(3-4): 351-3, 2012 Oct 12.
Article in English | MEDLINE | ID: mdl-22542523

ABSTRACT

According to the current taxonomy, the Staphylococcus intermedius group (SIG) comprises of at least three distinct species. While S. intermedius and S. pseudintermedius are associated with specific hosts (pigeons and dogs, respectively), the natural host of S. delphini remains unclear. We analysed 158 SIG isolates from less studied animal species belonging to the order Carnivora, including mink (n=118), fox (n=33), badger (n=6) and ferret (n=1). Species identification was performed by nuc PCR in combination with sodA sequence analysis and pta PCR restriction fragment length polymorphism (RFLP). The results showed a consistent association between host and bacterial species. All isolates from minks, ferret and badgers belonged to S. delphini group A, whereas all fox isolates except one were identified as S. pseudintermedius. The remaining fox isolate belonged to S. delphini group A. The results indicate that Mustelidae such as minks, ferrets and badgers are natural hosts of S. delphini group A. This is in contrast with Canidae, which are primarily colonized and infected with S. pseudintermedius. These findings suggest that coagulase-positive staphylococcal species may have evolved and diverged through host adaptation.


Subject(s)
Mustelidae/genetics , Mustelidae/microbiology , Staphylococcal Infections/veterinary , Staphylococcus intermedius/genetics , Staphylococcus intermedius/isolation & purification , Animals , Biological Evolution , Canidae/genetics , Canidae/microbiology , Polymerase Chain Reaction/veterinary , Polymorphism, Restriction Fragment Length , Staphylococcal Infections/microbiology , Staphylococcus intermedius/classification
12.
New Microbiol ; 34(4): 403-8, 2011 Oct.
Article in English | MEDLINE | ID: mdl-22143814

ABSTRACT

Among 97 bacterial isolates, 74 strains of Staphylococcus spp developed from 95 swabs taken from skin lesions in dogs. Twenty-eight staphylococcal strains resistant to methicillin and/or oxacillin were identified and mecA expression was confirmed for 14 of these strains. S. aureus and S. intermedius group (SIG) strains were particularly relevant in our cases due to their antibiotic resistance leading to an increased veterinary and public health risk. We suggest a diagnostic protocol based on cytological examination, bacterial identification to species level, and antibiotic sensitivity testing prior to prescribing antibiotic treatment for canine skin diseases.


Subject(s)
Anti-Bacterial Agents/pharmacology , Dermatitis , Dog Diseases/drug therapy , Dog Diseases/microbiology , Staphylococcal Skin Infections , Staphylococcus , Animals , Bacterial Proteins/genetics , Dermatitis/drug therapy , Dermatitis/microbiology , Dermatitis/veterinary , Dogs , Drug Resistance, Bacterial/genetics , Penicillin-Binding Proteins , Staphylococcal Skin Infections/drug therapy , Staphylococcal Skin Infections/microbiology , Staphylococcal Skin Infections/veterinary , Staphylococcus/drug effects , Staphylococcus/genetics , Staphylococcus/isolation & purification , Staphylococcus aureus/drug effects , Staphylococcus aureus/genetics , Staphylococcus aureus/isolation & purification , Staphylococcus epidermidis/drug effects , Staphylococcus epidermidis/genetics , Staphylococcus epidermidis/isolation & purification , Staphylococcus hominis/drug effects , Staphylococcus hominis/genetics , Staphylococcus hominis/isolation & purification , Staphylococcus intermedius/drug effects , Staphylococcus intermedius/genetics , Staphylococcus intermedius/isolation & purification
13.
J Vet Sci ; 12(3): 221-6, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21897094

ABSTRACT

The Staphylococcus (S.) intermedius group (SIG) has been a main research subject in recent years. S. pseudintermedius causes pyoderma and otitis in companion animals as well as foodborne diseases. To prevent SIG-associated infection and disease outbreaks, identification of both staphylococcal exotoxins and staphylococcal cassette chromosome mec (SCCmec) types among SIG isolates may be helpful. In this study, it was found that a single isolate (one out of 178 SIG isolates examined) harbored the canine enterotoxin SEC gene. However, the S. intermedius exfoliative toxin gene was found in 166 SIG isolates although the S. aureus-derived exfoliative toxin genes, such as eta, etb and etd, were not detected. SCCmec typing resulted in classifying one isolate as SCCmec type IV, 41 isolates as type V (including three S. intermedius isolates), and 10 isolates as non-classifiable. Genetic relatedness of all S. pseudintermedius isolates recovered from veterinary staff, companion animals, and hospital environments was determined by pulsed-field gel electrophoresis. Strains having the same band patterns were detected in S. pseudintermedius isolates collected at 13 and 18 months, suggesting possible colonization and/or expansion of a specific S. pseudintermedius strain in a veterinary hospital.


Subject(s)
Cat Diseases/microbiology , Dog Diseases/microbiology , Exotoxins/genetics , Staphylococcal Infections/veterinary , Staphylococcus intermedius/genetics , Staphylococcus intermedius/isolation & purification , Animals , Bacterial Toxins/genetics , Bacterial Toxins/metabolism , Cat Diseases/epidemiology , Cats , Chromosomes, Bacterial/genetics , Chromosomes, Bacterial/metabolism , Dog Diseases/epidemiology , Dogs , Electrophoresis, Gel, Pulsed-Field/veterinary , Enterotoxins/genetics , Enterotoxins/metabolism , Exfoliatins/genetics , Exfoliatins/metabolism , Exotoxins/metabolism , Hospitals, Animal , Humans , Medical Staff, Hospital , Molecular Sequence Data , Pets/microbiology , Polymerase Chain Reaction/veterinary , Republic of Korea/epidemiology , Staphylococcal Infections/epidemiology , Staphylococcal Infections/microbiology , Staphylococcus/genetics , Staphylococcus/isolation & purification
14.
Folia Microbiol (Praha) ; 56(5): 415-22, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21874595

ABSTRACT

The aim of this study was to determine the species distribution among 44 randomly selected clinical isolates (30 mecA-positive and 14 mecA-negative) of animal origin previously identified as Staphylococcus intermedius by phenotypic tests and species-specific PCR amplification of the 16S rRNA gene. For this purpose, we used a multiplex PCR for the detection of the nuc gene and restriction fragment length polymorphism analysis of pta gene amplified by PCR. Both methods allow discrimination of Staphylococcus pseudintermedius from the other closely related members of the S. intermedius group and other coagulase-positive staphylococci isolated from animals. Genetic diversity of S. pseudintermedius strains was analyzed by staphylococcal protein A-encoding gene (spa) typing. Multiplex PCR method was used to identify staphylococcal cassette chromosome mec (SCCmec) type in mecA-positive strains. All isolates previously identified as S. intermedius were shown to belong to S. pseudintermedius. According to PCR-based SCCmec typing, SCCmecIII was the most prevalent type (n = 23), and solely seven isolates were designated as non-typeable. Furthermore, the assessment of spa-typing results revealed that the majority of all strains (n = 27) harbored spa type t02, and 17 strains were classified as non-typeable.


Subject(s)
Animal Diseases/microbiology , Bacterial Typing Techniques , RNA, Ribosomal, 16S/analysis , Staphylococcal Infections/microbiology , Staphylococcal Infections/veterinary , Staphylococcus intermedius/isolation & purification , Animal Diseases/epidemiology , Animals , Bacterial Proteins/analysis , Bacterial Proteins/genetics , Body Fluids/microbiology , Cats , Cattle , Dogs , Drug Resistance, Bacterial , Gerbillinae , Micrococcal Nuclease/analysis , Micrococcal Nuclease/genetics , Multiplex Polymerase Chain Reaction , Penicillin-Binding Proteins , Poland/epidemiology , Polymorphism, Restriction Fragment Length , Rabbits , Staphylococcal Infections/epidemiology , Staphylococcal Protein A/analysis , Staphylococcal Protein A/genetics , Staphylococcus intermedius/classification , Staphylococcus intermedius/genetics , Staphylococcus intermedius/pathogenicity
15.
Infect Immun ; 79(8): 3074-86, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21576333

ABSTRACT

Cell wall-associated (CWA) proteins made by Gram-positive pathogens play a fundamental role in pathogenesis. Staphylococcus pseudintermedius is a major animal pathogen responsible for the canine skin disease bacterial pyoderma. Here, we describe the bioinformatic analysis of the family of 18 predicted CWA proteins encoded in the genome of S. pseudintermedius strain ED99 and determine their distribution among a phylogenetically diverse panel of S. pseudintermedius clinical isolates and closely related species of the Staphylococcus intermedius group. In parallel, we employed a proteomic approach to identify proteins presented on the surface of strain ED99 in vitro, revealing a total of 60 surface-localized proteins in one or more phases of growth, including 6 of the 18 genome-predicted CWA proteins. Based on these analyses, we selected two CWA proteins (SpsD and SpsL) encoded by all strains examined and investigated their capacity to mediate adherence to extracellular matrix proteins. We discovered that SpsD and SpsL mediated binding of a heterologous host, Lactococcus lactis, to fibrinogen and fibronectin and that SpsD mediated binding to cytokeratin 10, a major constituent of mammalian skin. Of note, the interaction with fibrinogen was host-species dependent, suggestive of a role for SpsD and SpsL in the host tropism of S. pseudintermedius. Finally, we identified IgG specific for SpsD and SpsL in sera from dogs with bacterial pyoderma, implying that both proteins are expressed during infection. The combined genomic and proteomic approach employed in the current study has revealed novel host-pathogen interactions which represent candidate therapeutic targets for the control of bacterial pyoderma.


Subject(s)
Adhesins, Bacterial/metabolism , Bacterial Proteins/analysis , Extracellular Matrix Proteins/metabolism , Host-Pathogen Interactions , Proteome/analysis , Staphylococcus intermedius/chemistry , Staphylococcus intermedius/genetics , Adhesins, Bacterial/genetics , Animals , Bacterial Proteins/genetics , Cell Wall/chemistry , Computational Biology , Dogs , Proteome/genetics
16.
J Vet Diagn Invest ; 23(2): 268-74, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21398446

ABSTRACT

The Staphylococcus intermedius bacterial group (SIG) includes 3 distinct genetically heterogenous species: S. intermedius, S. pseudintermedius, and S. delphini. This pathogen group is associated with many opportunistic skin and ear infections in companion animals. Human infections with S. intermedius and S. pseudintermedius isolates and the emergence of methicillin-resistant isolates have been recently reported, which emphasizes the importance of nationwide identification of SIG isolate prevalence and antibiotic resistance in veterinary clinics. In the present study, a total of 178 SIG isolates were obtained from veterinary staff (n  =  40), companion animals (n  =  115), and the local environment (n  =  23) in 8 Korean veterinary hospitals. Isolates were differentiated into 167 S. pseudintermedius (93.8%) and 11 S. intermedius (6.2%) isolates; S. delphini isolates were not identified. The most effective antibiotics against these isolates included amoxicillin-clavulanic acid, amikacin, nitrofloxacin, imipenem, and vancomycin; whereas ampicillin, penicillin, tetracycline, erythromycin, and trimethoprim-sulfamethoxazole were not effective. Surprisingly, the 128 SIG isolates (71.9%) displayed multiple drug resistance (MDR) against 3 or more antibiotic classes. Out of 52 SIG isolates carrying the methicillin-resistance gene (mecA), only 34 (65.4%) were oxacillin-resistant, and 49 (94.2%) methicillin-resistant SIG were multidrug resistant. This finding suggests the presence of greater numbers of MDR phenotypes than other isolates (P < 0.05).


Subject(s)
Cat Diseases/microbiology , Dog Diseases/microbiology , Pets/microbiology , Staphylococcal Infections/veterinary , Staphylococcus intermedius/isolation & purification , Zoonoses/microbiology , Animals , Cat Diseases/epidemiology , Cats , Chi-Square Distribution , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Dog Diseases/epidemiology , Dogs , Drug Resistance, Multiple, Bacterial , Hospitals, Animal , Humans , Korea/epidemiology , Microbial Sensitivity Tests/veterinary , Polymerase Chain Reaction/veterinary , Prevalence , Staphylococcal Infections/microbiology , Staphylococcus intermedius/genetics , Zoonoses/epidemiology
17.
Article in English | WPRIM (Western Pacific) | ID: wpr-108030

ABSTRACT

The Staphylococcus (S.) intermedius group (SIG) has been a main research subject in recent years. S. pseudintermedius causes pyoderma and otitis in companion animals as well as foodborne diseases. To prevent SIG-associated infection and disease outbreaks, identification of both staphylococcal exotoxins and staphylococcal cassette chromosome mec (SCCmec) types among SIG isolates may be helpful. In this study, it was found that a single isolate (one out of 178 SIG isolates examined) harbored the canine enterotoxin SEC gene. However, the S. intermedius exfoliative toxin gene was found in 166 SIG isolates although the S. aureus-derived exfoliative toxin genes, such as eta, etb and etd, were not detected. SCCmec typing resulted in classifying one isolate as SCCmec type IV, 41 isolates as type V (including three S. intermedius isolates), and 10 isolates as non-classifiable. Genetic relatedness of all S. pseudintermedius isolates recovered from veterinary staff, companion animals, and hospital environments was determined by pulsed-field gel electrophoresis. Strains having the same band patterns were detected in S. pseudintermedius isolates collected at 13 and 18 months, suggesting possible colonization and/or expansion of a specific S. pseudintermedius strain in a veterinary hospital.


Subject(s)
Animals , Cats , Dogs , Humans , Bacterial Toxins/genetics , Cat Diseases/epidemiology , Chromosomes, Bacterial/genetics , Dog Diseases/epidemiology , Electrophoresis, Gel, Pulsed-Field/veterinary , Enterotoxins/genetics , Exfoliatins/genetics , Exotoxins/genetics , Hospitals, Animal , Medical Staff, Hospital , Molecular Sequence Data , Pets/microbiology , Polymerase Chain Reaction/veterinary , Republic of Korea/epidemiology , Staphylococcal Infections/epidemiology , Staphylococcus/genetics , Staphylococcus intermedius/genetics
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