ABSTRACT
The enantioseparation of acidic and basic compounds can be successfully achieved in nonaqueous capillary electrophoresis using single-isomer charged ß-cyclodextrin (ß-CD) derivatives of opposite charge to that of the analytes. This chapter describes how to separate the enantiomers of three basic substances selected as model compounds, i.e., alprenolol, bupranolol, and terbutaline, using the negatively charged heptakis(2,3-di-O-acetyl-6-O-sulfo)-ß-CD. The enantiomers of three acidic drugs (tiaprofenic acid, suprofen, and flurbiprofen) are resolved using a monosubstituted amino ß-CD derivative, namely, 6-monodeoxy-6-mono(3-hydroxy)propylamino-ß-CD.
Subject(s)
Electrophoresis, Capillary/methods , beta-Cyclodextrins/analysis , beta-Cyclodextrins/chemistry , Alprenolol/analysis , Alprenolol/chemistry , Bupranolol/analysis , Bupranolol/chemistry , Flurbiprofen/analysis , Flurbiprofen/chemistry , Propionates/analysis , Propionates/chemistry , Stereoisomerism , Suprofen/analysis , Suprofen/chemistry , Temperature , Terbutaline/analysis , Terbutaline/chemistry , Ultraviolet RaysABSTRACT
Ionic liquids (ILs) appear really attractive as electrolyte additives in nonaqueous capillary electrophoresis (NACE). These salts may offer new possibilities of interactions to modulate analyte effective mobilities. The presence of 1-n-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide (BMIM NTf2) in acetonitrile/alcohol background electrolytes (BGEs) was investigated in this work. The aim of this study was to elucidate the influence of the IL concentration on the electrophoretic behavior of four arylpropionic acids and to identify the interactions between the analytes and the IL cation. The influence on mobility of the IL concentration, the nature and the proportion of the organic solvents, and the concentration of the ionic components of the BGE was first studied by a univariate approach. A four-factor D-optimal experimental design was then applied to provide a deeper insight into analyte interaction with IL cation present both free in BGE and adsorbed onto the capillary wall.
Subject(s)
Electrophoresis, Capillary/methods , Ionic Liquids/chemistry , Phenylpropionates/analysis , Propionates/analysis , Carbazoles/analysis , Carbazoles/chemistry , Electrolytes/chemistry , Ketoprofen/analysis , Ketoprofen/chemistry , Models, Chemical , Molecular Structure , Multivariate Analysis , Naproxen/analysis , Naproxen/chemistry , Phenylpropionates/chemistry , Propionates/chemistry , Stereoisomerism , Suprofen/analysis , Suprofen/chemistryABSTRACT
A rapid, simple, stability-indicating assay procedure for suprofen, a new analgesic agent, in suprofen drug substance and in capsules was developed using high-performance liquid chromatography. Suprofen was extracted from the sample matrix with methanol and diluted with internal standard solution, and an aliquot was chromatographed on a reversed-phased column using acetonitrile-pH 3.0 buffer solution as the mobile phase. The selectivity of te chromatographic system for intact suprofen was demonstrated by resolving suprofen from synthetic intermediates, potential impurities, and reaction products resulting from accelerated stress conditions. The method is linear, quantitative, and reproducible. Either peak height or peak area ratios can be used for quantitation.
