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1.
Nat Commun ; 5: 5467, 2014 Nov 17.
Article in English | MEDLINE | ID: mdl-25399868

ABSTRACT

Telomeres protect the ends of linear genomes, and the gradual loss of telomeres is associated with cellular ageing. Telomere protection involves the insertion of the 3' overhang facilitated by telomere repeat-binding factor 2 (TRF2) into telomeric DNA, forming t-loops. We present evidence suggesting that t-loops can also form at interstitial telomeric sequences in a TRF2-dependent manner, forming an interstitial t-loop (ITL). We demonstrate that TRF2 association with interstitial telomeric sequences is stabilized by co-localization with A-type lamins (lamin A/C). We also find that lamin A/C interacts with TRF2 and that reduction in levels of lamin A/C or mutations in LMNA that cause an autosomal dominant premature ageing disorder--Hutchinson Gilford Progeria Syndrome (HGPS)-lead to reduced ITL formation and telomere loss. We propose that cellular and organismal ageing are intertwined through the effects of the interaction between TRF2 and lamin A/C on chromosome structure.


Subject(s)
Chromosomes, Human/physiology , Lamin Type A/physiology , TATA Box Binding Protein-Like Proteins/physiology , Cellular Senescence/physiology , Fibroblasts/physiology , Humans , In Situ Hybridization, Fluorescence , Progeria/etiology , Telomere/physiology
2.
J Biol ; 8(11): 97, 2009.
Article in English | MEDLINE | ID: mdl-19951399

ABSTRACT

The complexity of the core promoter transcription machinery has emerged as an additional level of transcription regulation that is used during vertebrate development. Recent studies, including one published in BMC Biology, provide mechanistic insights into how the TATA binding protein (TBP) and its vertebrate-specific paralog TBP2 (TRF3) switch function during the transition from the oocyte to the embryo. See research article http://www.biomedcentral.com/1741-7007/7/45.


Subject(s)
Germ Cells/physiology , Nuclear Proteins/physiology , Oocytes/physiology , TATA Box Binding Protein-Like Proteins/physiology , Transcription Factors/physiology , Animals , Female , Germ Cells/metabolism , Humans , Nuclear Proteins/genetics , Oocytes/metabolism , TATA Box Binding Protein-Like Proteins/genetics , Transcription Factors/genetics
3.
Article in English | MEDLINE | ID: mdl-19022758

ABSTRACT

Sequence-specific enhancer-binding transcription factors and chromatin-modifying proteins are well recognized for their potential contributions to cell-type-specific gene regulation. In contrast, the role of core promoter recognition factors, such as TFIID in modulating gene- and cell-type-specific programs of transcription has been less understood. In general, the so-called basal factors have largely been relegated to a supporting role as invariant components of the preinitiation complex. To dissect the potential contributions of TFIID to cell-type-specific transcription, we have studied the developmental process of skeletal myogenesis. Terminal differentiation during myogenesis involves an intricate reprogramming of transcription that is thought to be directed by cell-type-specific transcription regulatory factors. Here, we summarize our findings that the canonical TFIID complex must first be dismantled as a requisite step during the differentiation of myoblasts into myotubes and subsequently substituted by a novel core transcription complex composed of TAF3 and TRF3. Although this remarkable mechanism of completely switching core promoter recognition complexes to drive terminal differentiation has not been previously documented, it may eventually prove to be the rule rather than the exception as we learn more about cell-type-specific gene regulation.


Subject(s)
Muscle Development/genetics , Muscle Development/physiology , Muscle, Skeletal/growth & development , Transcription Factors/genetics , Transcription Factors/physiology , Animals , Cell Differentiation/genetics , Cell Differentiation/physiology , Gene Expression Regulation, Developmental , Genes, Switch , Humans , Models, Biological , Muscle Fibers, Skeletal/cytology , Muscle Fibers, Skeletal/physiology , Muscle, Skeletal/physiology , Myoblasts, Skeletal/cytology , Myoblasts, Skeletal/physiology , Promoter Regions, Genetic , TATA Box Binding Protein-Like Proteins/genetics , TATA Box Binding Protein-Like Proteins/physiology , Transcription Factor TFIID/genetics , Transcription Factor TFIID/physiology
4.
J Hypertens ; 25(11): 2185-92, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17921808

ABSTRACT

Aging is a major risk factor for hypertension and associated cardiovascular disease. In most proliferative tissues, aging is characterized by shortening of the DNA component of telomeres, the specialized genetic segments that cap the end of eukaryotic chromosomes and protect them from end-to-end fusions. By inducing genomic instability, replicative senescence and apoptosis, telomere shortening is thought to contribute to organismal aging and to the development of age-related diseases. Here, we review animal and human studies that have investigated the possible links between telomere ablation and the pathogenesis of hypertension and related target organ damage. Although evidence is mounting that alterations in telomerase activity and telomere shortening may play a role in the pathogenesis of hypertension, additional studies are required to understand the molecular mechanisms by which telomere dysfunction and hypertension are functionally connected. As our knowledge on this emerging field grows, the challenge will be to ascertain whether all this information might translate into clinical applications.


Subject(s)
Hypertension/genetics , Telomere , Aging/physiology , Aspartic Acid Endopeptidases/genetics , Blood Pressure , Endothelin-Converting Enzymes , Humans , Hypertension/complications , Hypertension/etiology , Leukocytes/metabolism , Metalloendopeptidases/genetics , Muscle, Smooth, Vascular/pathology , Nuclear Proteins/physiology , Oxidative Stress , TATA Box Binding Protein-Like Proteins/physiology , Telomerase/physiology , Telomeric Repeat Binding Protein 2
5.
Genes Dev ; 21(17): 2137-49, 2007 Sep 01.
Article in English | MEDLINE | ID: mdl-17704303

ABSTRACT

Transcriptional mechanisms that govern cellular differentiation typically include sequence-specific DNA-binding proteins and chromatin-modifying activities. These regulatory factors are assumed necessary and sufficient to drive both divergent programs of proliferation and terminal differentiation. By contrast, potential contributions of the basal transcriptional apparatus to orchestrate cell-specific gene expression have been poorly explored. In order to probe alternative mechanisms that control differentiation, we have assessed the fate of the core promoter recognition complex, TFIID, during skeletal myogenesis. Here we report that differentiation of myoblast to myotubes involves the disruption of the canonical holo-TFIID and replacement by a novel TRF3/TAF3 (TBP-related factor 3/TATA-binding protein-associated factor 3) complex. This required switching of core promoter complexes provides organisms a simple yet effective means to selectively turn on one transcriptional program while silencing many others. Although this drastic but parsimonious transcriptional switch had previously escaped our attention, it may represent a more general mechanism for regulating cell type-specific terminal differentiation.


Subject(s)
Cell Differentiation , Gene Expression Regulation, Developmental , Muscle Development/genetics , Nuclear Proteins/physiology , TATA Box Binding Protein-Like Proteins/physiology , Transcription Factor TFIID/physiology , Transcription, Genetic , Animals , Cell Line , Humans , Mice , Models, Genetic , Muscle Fibers, Skeletal/metabolism , Muscle, Skeletal/embryology , Myofibrils/metabolism , RNA Interference , TATA-Binding Protein Associated Factors
6.
Proc Natl Acad Sci U S A ; 104(32): 13068-73, 2007 Aug 07.
Article in English | MEDLINE | ID: mdl-17670947

ABSTRACT

TRF2 (telomeric repeat binding factor 2) is an essential component of the telomeric cap, where it forms and stabilizes the T-loop junctions. TRF2 forms the T-loops by stimulating strand invasion of the 3' overhang into duplex DNA. TRF2 also has been shown to localize to nontelomeric DNA double-strand breaks, but its functional role in DNA repair has not been examined. Here, we present evidence that TRF2 is involved in homologous recombination (HR) repair of nontelomeric double-strand breaks. Depletion of TRF2 strongly inhibited HR and delayed the formation of Rad51 foci after gamma-irradiation, whereas overexpression of TRF2 stimulated HR. Depletion of TRF2 had no effect on nonhomologous end-joining, and overexpression of TRF2 inhibited nonhomologous end-joining. We propose, based on our results and on the ability of TRF2 to mediate strand invasion, that TRF2 plays an essential role in HR by facilitating the formation of early recombination intermediates.


Subject(s)
DNA Breaks, Double-Stranded , DNA Repair , Nuclear Proteins/physiology , Recombination, Genetic , TATA Box Binding Protein-Like Proteins/physiology , Cells, Cultured , Humans , Rad51 Recombinase/analysis , Telomeric Repeat Binding Protein 2
7.
Reproduction ; 134(1): 51-62, 2007 Jul.
Article in English | MEDLINE | ID: mdl-17641088

ABSTRACT

Gametogenesis, the process during which germ cells are generated is essential for reproduction. In mammals, maternal mRNA and proteins present in the oocyte are required to ensure the progression of development until the embryo activates its genome after fertilisation. It is well established that the oocyte synthesises these maternal factors during oocyte growth and then undergoes a quiescent transcriptional period that will be resumed only after fertilisation. However, the mechanisms that govern transcriptional regulation and subsequent silencing during oogenesis are not well understood. Here, we have examined the expression and localisation of the TATA-binding protein (TBP) and the related protein TBP2 (also called TRF3, TBP-related factor 3) during oogenesis and in early mouse embryos. We show that TBP is expressed in the oocytes at the beginning of folliculogenesis, but it is undetectable during further stages of oocyte development, and becomes abundant again only after fertilisation. In contrast to TBP, we found that TBP2 is highly expressed in growing oocytes during folliculogenesis, declines upon ovulation, and is almost undetectable after fertilisation by the two-cell stage. The mirroring localisation profile of TBP and TBP2 suggests different roles for the two proteins in establishing specialised programs of gene expression during oocyte development and in early mouse embryos. Analysis of mutant mouse ovaries in which oocyte-specific factors have been knocked-out suggests that TBP2 is a potential candidate for regulating transcriptional control of oogenesis. Moreover, our results obtained with oocytes lacking the oocyte-specific nuclear chaperone nucleoplasmin 2 suggest that TBP2 function may be related to non-condensed chromatin conformation.


Subject(s)
Gene Expression Regulation, Developmental , Oogenesis/physiology , TATA Box Binding Protein-Like Proteins/physiology , TATA-Box Binding Protein/physiology , 3T3 Cells , Animals , Antibodies, Monoclonal/isolation & purification , Antibodies, Monoclonal/pharmacology , Blotting, Western , Embryonic Development/genetics , Female , Fluorescent Antibody Technique , Gene Expression , Mice , Mice, Inbred C57BL , Mice, Knockout , Oocytes/metabolism , Ovarian Follicle/physiology , Transcription, Genetic/physiology , Transfection/methods
8.
Nat Struct Mol Biol ; 14(8): 754-61, 2007 Aug.
Article in English | MEDLINE | ID: mdl-17632522

ABSTRACT

The shelterin complex at mammalian telomeres contains the single-stranded DNA-binding protein Pot1, which regulates telomere length and protects chromosome ends. Pot1 binds Tpp1, the shelterin component that connects Pot1 to the duplex telomeric DNA-binding proteins Trf1 and Trf2. Control of telomere length requires that Pot1 binds Tpp1 as well as the single-stranded telomeric DNA, but it is not known whether the protective function of Pot1 depends on Tpp1. Alternatively, Pot1 might function similarly to the Pot1-like proteins of budding and fission yeast, which have no known Tpp1-like connection to the duplex telomeric DNA. Using mutant mouse cells with diminished Tpp1 levels, RNA interference directed to mouse Tpp1 and Pot1, and complementation of mouse Pot1 knockout cells with human and mouse Pot1 variants, we show here that Tpp1 is required for the protective function of mammalian Pot1 proteins.


Subject(s)
DNA-Binding Proteins/physiology , Telomere-Binding Proteins/metabolism , Telomere-Binding Proteins/physiology , Telomere/metabolism , Animals , Cells, Cultured , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Humans , Mice , Nuclear Proteins/antagonists & inhibitors , Nuclear Proteins/physiology , RNA Interference , Shelterin Complex , TATA Box Binding Protein-Like Proteins/antagonists & inhibitors , TATA Box Binding Protein-Like Proteins/physiology , Telomere-Binding Proteins/genetics , Telomeric Repeat Binding Protein 2
9.
Mol Cell ; 26(3): 323-34, 2007 May 11.
Article in English | MEDLINE | ID: mdl-17499040

ABSTRACT

The mechanisms by which telomeres are distinguished from DNA double-strand breaks are poorly understood. Here we have defined the minimal requirements for the protection of telomeric DNA ends from nonhomologous end-joining (NHEJ). Neither long, single-stranded overhangs nor t loop formation is essential to prevent NHEJ-mediated ligation of telomeric ends in vitro. Instead, a tandem array of 12 telomeric repeats is sufficient to impede illegitimate repair in a highly directional manner at nearby DNA ends. The polarity of end protection is consistent with the orientation of naturally occurring telomeres and is well suited to minimize interference between chromosome capping and the repair of DNA double-strand breaks in subtelomeric sequences. Biochemical fractionation and reconstitution revealed that telomere protection is mediated by a RAP1/TRF2 complex, providing evidence for a direct role for human RAP1 in the protection of telomeric DNA from NHEJ.


Subject(s)
DNA Breaks, Double-Stranded , DNA Repair , Directed Molecular Evolution/methods , Nuclear Proteins/physiology , TATA Box Binding Protein-Like Proteins/physiology , Telomere-Binding Proteins/physiology , Telomere/metabolism , Cells, Cultured , Chromosomal Instability/physiology , DNA-Binding Proteins/metabolism , Evolution, Molecular , HeLa Cells , Humans , Models, Biological , Shelterin Complex , Telomere-Binding Proteins/metabolism , Telomeric Repeat Binding Protein 2
10.
Mech Ageing Dev ; 128(4): 340-5, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17395247

ABSTRACT

Repair of single strand breaks in telomeric DNA is less efficient than in other genomic regions. This leads to an increased vulnerability of telomeric DNA towards damage induced by reactive oxygen species (ROS) and to accelerated telomere shortening under oxidative stress. The causes for the diminished repair efficacy in telomeres are unknown. We show here that overexpression of the telomere-binding protein TRF2 further reduces telomeric, but not genomic, single strand break repair. This suggests the possibility of strand break repair in telomeres being sterically hindered by the three-dimensional structure of the telomere DNA-protein complex and explains the effect of TRF2 on telomere shortening rates in telomerase-negative cells.


Subject(s)
DNA Breaks, Single-Stranded , DNA Repair/genetics , Fibroblasts/metabolism , Nuclear Proteins/biosynthesis , Nuclear Proteins/genetics , TATA Box Binding Protein-Like Proteins/biosynthesis , TATA Box Binding Protein-Like Proteins/genetics , Telomere/genetics , Cell Line , Humans , Nuclear Proteins/physiology , TATA Box Binding Protein-Like Proteins/physiology , Telomere/metabolism , Telomeric Repeat Binding Protein 2
11.
Proc Natl Acad Sci U S A ; 103(32): 11874-9, 2006 Aug 08.
Article in English | MEDLINE | ID: mdl-16880378

ABSTRACT

Mammalian telomeric proteins function through dynamic interactions with each other and telomere DNA. We previously reported the formation of a high-molecular-mass telomeric complex (the mammalian telosome) that contains the six core proteins TRF1, TRF2, RAP1, TIN2, POT1, and TPP1 (formerly named PTOP/PIP1/TINT1) and mediates telomere end-capping and length control. In this report, we sought to elucidate the mechanism of six-protein complex (or shelterin) formation and the function of this complex. Through reconstitution experiments, we demonstrate here that TIN2 and TPP1 are key components in mediating the six-protein complex assembly. We demonstrate that not only TIN2 but also TPP1 are required to bridge the TRF1 and TRF2 subcomplexes. Specifically, TPP1 helps to stabilize the TRF1-TIN2-TRF2 interaction and promote six-protein complex formation. Consistent with this model, overexpression of TPP1 enhanced TIN2-TRF2 association. Conversely, knocking down TPP1 reduced the ability of endogenous TRF1 to associate with the TRF2 complex. Our results suggest that coordinated interactions among TPP1, TIN2, TRF1, and TRF2 may ensure robust assembly of the telosome, telomere targeting of its subunits, and, ultimately, regulated telomere maintenance.


Subject(s)
Telomere-Binding Proteins/physiology , Telomere/metabolism , Animals , HeLa Cells , Humans , Insecta , Models, Biological , Nuclear Proteins/physiology , Protein Binding , Protein Structure, Tertiary , Shelterin Complex , TATA Box Binding Protein-Like Proteins/physiology , Telomere/chemistry , Telomere/ultrastructure , Telomere-Binding Proteins/chemistry , Telomeric Repeat Binding Protein 1/physiology , Telomeric Repeat Binding Protein 2
13.
PLoS Biol ; 2(8): E240, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15314656

ABSTRACT

The telomeric protein TRF2 is required to prevent mammalian telomeres from activating DNA damage checkpoints. Here we show that overexpression of TRF2 affects the response of the ATM kinase to DNA damage. Overexpression of TRF2 abrogated the cell cycle arrest after ionizing radiation and diminished several other readouts of the DNA damage response, including phosphorylation of Nbs1, induction of p53, and upregulation of p53 targets. TRF2 inhibited autophosphorylation of ATM on S1981, an early step in the activation of this kinase. A region of ATM containing S1981 was found to directly interact with TRF2 in vitro, and ATM immunoprecipitates contained TRF2. We propose that TRF2 has the ability to inhibit ATM activation at telomeres. Because TRF2 is abundant at chromosome ends but not elsewhere in the nucleus, this mechanism of checkpoint control could specifically block a DNA damage response at telomeres without affecting the surveillance of chromosome internal damage.


Subject(s)
Cell Cycle Proteins/metabolism , DNA Damage , DNA-Binding Proteins/metabolism , Nuclear Proteins/physiology , Protein Serine-Threonine Kinases/metabolism , TATA Box Binding Protein-Like Proteins/physiology , Telomere/ultrastructure , Tumor Suppressor Proteins/metabolism , Ataxia Telangiectasia Mutated Proteins , Cell Cycle , Cell Line, Tumor , Chromosomes/ultrastructure , Dimerization , Enzyme Activation , Glutathione Transferase/metabolism , Humans , Immunoblotting , Immunoprecipitation , Phosphorylation , Protein Binding , Radiation, Ionizing , Telomeric Repeat Binding Protein 2 , Transfection , Tumor Suppressor Protein p53/metabolism , Up-Regulation
14.
J Biol Chem ; 279(15): 15339-47, 2004 Apr 09.
Article in English | MEDLINE | ID: mdl-14726532

ABSTRACT

The general transcription factor TFIID sets the mRNA start site and consists of TATA-binding protein and associated factors (TAF(II)s), some of which are also present in SPT-ADA-GCN5 (SAGA)-related complexes. In yeast, results of multiple studies indicate that TFIID-specific TAF(II)s are not required for the transcription of most genes, implying that intact TFIID may have a surprisingly specialized role in transcription. Relatively little is known about how TAF(II)s contribute to metazoan transcription in vivo, especially at developmental and tissue-specific genes. Previously, we investigated functions of four shared TFIID/SAGA TAF(II)s in Caenorhabditis elegans. Whereas TAF-4 was required for essentially all embryonic transcription, TAF-5, TAF-9, and TAF-10 were dispensable at multiple developmental and other metazoan-specific promoters. Here we show evidence that in C. elegans embryos transcription of most genes requires TFIID-specific TAF-1. TAF-1 is not as universally required as TAF-4, but it is essential for a greater proportion of transcription than TAF-5, -9, or -10 and is important for transcription of many developmental and other metazoan-specific genes. TAF-2, which binds core promoters with TAF-1, appears to be required for a similarly substantial proportion of transcription. C. elegans TAF-1 overlaps functionally with the coactivator p300/CBP (CBP-1), and at some genes it is required along with the TBP-like protein TLF(TRF2). We conclude that during C. elegans embryogenesis TAF-1 and TFIID have broad roles in transcription and development and that TFIID and TLF may act together at certain promoters. Our findings imply that in metazoans TFIID may be of widespread importance for transcription and for expression of tissue-specific genes.


Subject(s)
Caenorhabditis elegans Proteins/physiology , Caenorhabditis elegans/embryology , Chromosomal Proteins, Non-Histone/physiology , Transcription Factor TFIID/chemistry , Transcription Factors/physiology , Transcription, Genetic , Animals , Caenorhabditis elegans Proteins/metabolism , Chromosomal Proteins, Non-Histone/metabolism , DNA-Binding Proteins , Gene Expression Regulation, Developmental , Green Fluorescent Proteins , Histone Chaperones , Humans , Immunoblotting , Luminescent Proteins/metabolism , Microscopy, Fluorescence , Models, Biological , Models, Genetic , Nuclear Proteins/metabolism , Phenotype , Phosphorylation , Phylogeny , Promoter Regions, Genetic , Protein Structure, Tertiary , RNA Interference , Recombinant Fusion Proteins/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/metabolism , TATA Box Binding Protein-Like Proteins/physiology , TATA-Binding Protein Associated Factors/metabolism , Trans-Activators/metabolism , Transcription Factor TFIID/metabolism , Transcription Factors/metabolism
15.
Proc Natl Acad Sci U S A ; 100(25): 14887-91, 2003 Dec 09.
Article in English | MEDLINE | ID: mdl-14634207

ABSTRACT

TATA-box-binding protein (TBP) is a highly conserved RNA polymerase II general transcription factor that binds to the core promoter and initiates assembly of the preinitiation complex. Two proteins with high homology to TBP have been found: TBP-related factor 1 (TRF1), described only in Drosophila melanogaster, and TRF2, which is broadly distributed in metazoans. Here, we report the identification and characterization of an additional TBP-related factor, TRF3. TRF3 is virtually identical to TBP in the C-terminal core domain, including all residues involved in DNA binding and interaction with other general transcription factors. Like other TBP family members, the N-terminal region of TRF3 is divergent. The TRF3 gene is present and expressed in vertebrates, from fish through humans, but absent from the genomes of the urochordate Ciona intestinalis and the lower eukaryotes D. melanogaster and Caenorhabditis elegans. TRF3 is a nuclear protein that is present in all human and mouse tissues and cell lines examined. Despite the highly homologous TBP-like C-terminal core domain, gel filtration analysis indicates that the native molecular weight of TRF3 is substantially less than that of TFIID. Interestingly, after mitosis, reimport of TRF3 into the nucleus occurs subsequent to TBP and other basal transcription factors. In summary, TRF3 is a highly conserved vertebrate-specific TRF whose phylogenetic conservation, expression pattern, and other properties are distinct from those of TBP and all other TRFs.


Subject(s)
TATA Box Binding Protein-Like Proteins/metabolism , TATA Box Binding Protein-Like Proteins/physiology , Transcription Factors/metabolism , Transcription Factors/physiology , Amino Acid Sequence , Animals , Caenorhabditis elegans/metabolism , Cell Line , Cell Nucleus/metabolism , Chromatography, Gel , Ciona intestinalis/metabolism , Computational Biology , DNA/chemistry , Drosophila melanogaster/metabolism , Fluorescent Antibody Technique, Indirect , HeLa Cells , Humans , Mice , Mitosis , Molecular Sequence Data , Nuclear Proteins , Phylogeny , Protein Biosynthesis , Protein Structure, Tertiary , RNA Polymerase II/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Sequence Homology, Amino Acid , TATA Box Binding Protein-Like Proteins/chemistry , Tissue Distribution , Transcription Factor TFIID/chemistry , Transcription Factors/chemistry , Transcription, Genetic
16.
Mol Cell Biol ; 23(12): 4107-20, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12773555

ABSTRACT

The TATA-binding protein (TBP) is a universal transcription factor required for all of the eukaryotic RNA polymerases. In addition to TBP, metazoans commonly express a distantly TBP-related protein referred to as TBP-like protein (TLP/TRF2/TLF). Although the function of TLP in transcriptional regulation is not clear, it is known that TLP is required for embryogenesis and spermiogenesis. In the present study, we investigated the cellular functions of TLP by using TLP knockout chicken DT40 cells. TLP was found to be dispensable for cell growth. Unexpectedly, TLP-null cells exhibited a 20% elevated cell cycle progression rate that was attributed to shortening of the G(2) phase. This indicates that TLP functions as a negative regulator of cell growth. Moreover, we found that TLP mainly existed in the cytoplasm and was translocated to the nucleus restrictedly at the G(2) phase. Ectopic expression of nuclear localization signal-carrying TLP resulted in an increase (1.5-fold) in the proportion of cells remaining in the G(2)/M phase and apoptotic state. Notably, TLP-null cells showed an insufficient G(2) checkpoint when the cells were exposed to stresses such as UV light and methyl methanesulfonate, and the population of apoptotic cells after stresses decreased to 40%. These phenomena in G(2) checkpoint regulation are suggested to be p53 independent because p53 does not function in DT40 cells. Moreover, TLP was transiently translocated to the nucleus shortly (15 min) after stress treatment. The expression of several stress response and cell cycle regulatory genes drifted in a both TLP- and stress-dependent manner. Nucleus-translocating TLP is therefore thought to work by checking cell integrity through its transcription regulatory ability. TLP is considered to be a signal-transducing transcription factor in cell cycle regulation and stress response.


Subject(s)
Cell Cycle , G2 Phase , TATA Box Binding Protein-Like Proteins/physiology , Active Transport, Cell Nucleus , Animals , Apoptosis , Blotting, Northern , Cell Division , Cell Line , Cell Nucleus/metabolism , Cell Separation , Chickens , Cytoplasm/metabolism , Flow Cytometry , Immunohistochemistry , Nuclear Localization Signals , Plasmids/metabolism , Protein Transport , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Stress, Physiological , Time Factors , Transcription Factors/metabolism , Transcription, Genetic , Transfection , Ultraviolet Rays
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