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1.
Arch Virol ; 165(6): 1501-1504, 2020 Jun.
Article in English | MEDLINE | ID: mdl-32266551

ABSTRACT

We provide the complete sequence of a virus tentatively named "Tetranychus urticae-associated picorna-like virus 1PK13" (TuaPV1-PK13) obtained from the high-throughput sequencing of a symptomless apple leaf sample. Although the virus sequence was originally derived from apple leaves, the data suggest that the virus is associated with the two-spotted mite Tetranychus urticae.


Subject(s)
Malus/virology , Picornaviridae/classification , Plant Leaves/virology , Tetranychidae/virology , Animals , High-Throughput Nucleotide Sequencing , Picornaviridae/isolation & purification , Whole Genome Sequencing
2.
J Invertebr Pathol ; 166: 107228, 2019 09.
Article in English | MEDLINE | ID: mdl-31386829

ABSTRACT

As a polyphagous herbivore, the two-spotted spider mite Tetranychus urticae is engaged with various plant hosts and interacts with diverse organisms that share the same ecological niche. Thus, T. urticae faces frequent challenges from viral infections. However, the RNA viruses of T. urticae are still unknown. Here, we constructed two libraries (~8 Gb for RNA and ~10 Mb for small RNA) from a strain of T. urticae using deep sequencing, and identified three novel RNA viruses from the families Kitaviridae, Dicistroviridae, and Chuviridae. Among them, the Kitaviridae and Dicistroviridae viruses presented a possible interaction pattern with the host RNA interference pathway.


Subject(s)
Host-Parasite Interactions/physiology , RNA Viruses/genetics , Tetranychidae/virology , Animals , RNA Interference
3.
Viruses ; 11(3)2019 03 07.
Article in English | MEDLINE | ID: mdl-30866521

ABSTRACT

Viral metagenomics and high throughput sequence mining have revealed unexpected diversity, and the potential presence, of parvoviruses in animals from all phyla. Among arthropods, this diversity highlights the poor knowledge that we have regarding the evolutionary history of densoviruses. The aim of this study was to explore densovirus diversity in a small arthropod pest belonging to Acari, the two-spotted spider mite Tetranychus urticae, while using viral metagenomics based on virus-enrichment. Here, we present the viromes obtained from T. urticae laboratory populations made of contigs that are attributed to nine new potential viral species, including the complete sequence of a novel densovirus. The genome of this densovirus has an ambisens genomic organization and an unusually compact size with particularly small non-structural proteins and a predicted major capsid protein that lacks the typical PLA2 motif that is common to all ambidensoviruses described so far. In addition, we showed that this new densovirus had a wide prevalence across populations of mite species tested and a genomic diversity that likely correlates with the host phylogeny. In particular, we observed a low densovirus genomic diversity between the laboratory and natural populations, which suggests that virus within-species evolution is probably slower than initially thought. Lastly, we showed that this novel densovirus can be inoculated to the host plant following feeding by infected mites, and circulate through the plant vascular system. These findings offer new insights into densovirus prevalence, evolution, and ecology.


Subject(s)
Densovirus/genetics , Densovirus/isolation & purification , Genetic Variation , Microbiota , Tetranychidae/virology , Animals , Capsid Proteins/genetics , Female , Genome, Viral , Metagenomics , Phylogeny , Plants/virology , Prevalence , Viral Nonstructural Proteins/genetics
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