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1.
Sci Rep ; 12(1): 1022, 2022 01 19.
Article in English | MEDLINE | ID: mdl-35046482

ABSTRACT

Adult hippocampal neurogenesis (AHN) plays an important role in hippocampus-dependent function. The number of doublecortin (Dcx)-positive immature neurons in the dentate gyrus decreases over time, especially in the early stages of Alzheimer's disease (AD), and is further reduced in later stages of AD. Obesity in midlife is associated with dementia later in life; however, the underlying mechanisms by which obesity results in the development of dementia later in life remain unknown. Here, we show that endoplasmic reticulum (ER) stress was activated in the hippocampus and processes of Dcx-expressing immature neurons were shortened, coexpressing CHOP in APP23 AD model mice with high-fat diet-induced long-term obesity and in aged Leprdb/db (db/db) mice. Moreover, in cells differentiating from hippocampal neurospheres, Dcx mRNA was rapidly degraded via a microRNA (miRNA) pathway after thapsigargin treatment in vitro. These results indicate that loss of Dcx mRNA induced by ER stress during AHN may cause memory impairment in obese individuals later in life.


Subject(s)
Doublecortin Domain Proteins/metabolism , Endoplasmic Reticulum Stress/physiology , Neurons/metabolism , Obesity/complications , Aging , Animals , Behavior, Animal/physiology , Cells, Cultured , Diet, High-Fat , Disease Models, Animal , Doublecortin Domain Proteins/genetics , Hippocampus , Memory , Mice , Mice, Inbred C57BL , Mice, Transgenic , Obesity/physiopathology , Thapsigargin/administration & dosage
2.
Aging (Albany NY) ; 11(21): 9875-9892, 2019 11 12.
Article in English | MEDLINE | ID: mdl-31714893

ABSTRACT

Pathophysiological states cause misfolded protein accumulation in the endoplasmic reticulum (ER). Then, ER stress and the unfolded protein response (UPR) are activated. Targeting ER stress may enhance the adaptive UPR and then protect the cell against pathogenic environments. In the present study, we utilized nanotechnology to synthesize thapsigargin nanoparticles (TG NPs) which induced ER stress and the UPR pathway, to study the role of ER stress and autophagy in chronic kidney disease (CKD). We found that the mRNA levels of ER stress- and autophagy-related molecules were elevated in the renal tissue of CKD patients compared to those of healthy individuals. Furthermore, TG NPs induced the UPR pathway and autophagy in HK-2 human kidney tubular epithelial cells. TG NPs protected HK-2 cells against oxidative stress-induced cell death through the activation of Nrf2 and FoxO1. The siRNA-mediated inhibition of Nrf2 or FoxO1 resulted in enhanced oxidative stress-induced cytotoxicity in HK-2 cells. In a mouse model of adenine diet-induced CKD, TG NPs and KIM-1-TG NPs ameliorated renal injury through the stimulation of ER stress and its downstream pathways. Our findings suggest that the induction of ER stress using pharmacological agents may offer a promising therapeutic strategy for preventing or interfering with CKD progression.


Subject(s)
Forkhead Box Protein O1/metabolism , NF-E2-Related Factor 2/metabolism , Nanoparticles/chemistry , Renal Insufficiency, Chronic/drug therapy , Thapsigargin/administration & dosage , Adenine , Animals , Autophagy/drug effects , Autophagy/genetics , Cell Line , Drug Evaluation, Preclinical , Endoplasmic Reticulum Stress/drug effects , Endoplasmic Reticulum Stress/genetics , Humans , Male , Mice , Mice, Inbred C57BL , Nanoparticles/therapeutic use , Nanoparticles/ultrastructure , Renal Insufficiency, Chronic/metabolism
3.
Ann Med ; 50(5): 381-390, 2018 08.
Article in English | MEDLINE | ID: mdl-29895209

ABSTRACT

Acute kidney injury (AKI) is a medical condition characterized by kidney damage with a rapid decline of renal function, which is associated with high mortality and morbidity. Recent research has further established an intimate relationship between AKI and chronic kidney disease. Perturbations of kidney cells in AKI result in the accumulation of unfolded and misfolded proteins in the endoplasmic reticulum (ER), leading to unfolded protein response (UPR) or ER stress. In this review, we analyze the role and regulation of ER stress in AKI triggered by renal ischemia-reperfusion and cisplatin nephrotoxicity. The balance between the two major components of UPR, the adaptive pathway and the apoptotic pathway, plays a critical role in determining the cell fate in ER stress. The adaptive pathway is evoked to attenuate translation, induce chaperones, maintain protein homeostasis and promote cell survival. Prolonged ER stress activates the apoptotic pathway, resulting in the elimination of dysfunctional cells. Therefore, regulating ER stress in kidney cells may provide a therapeutic target in AKI. KEY MESSAGES Perturbations of kidney cells in acute kidney injury result in the accumulation of unfolded and misfolded proteins in ER, leading to unfolded protein response (UPR) or ER stress. The balance between the adaptive pathway and the apoptotic pathway of UPR plays a critical role in determining the cell fate in ER stress. Modulation of ER stress in kidney cells may provide a therapeutic strategy for acute kidney injury.


Subject(s)
Acute Kidney Injury/pathology , Endoplasmic Reticulum Stress/drug effects , Kidney/pathology , Renal Insufficiency, Chronic/pathology , Acute Kidney Injury/chemically induced , Acute Kidney Injury/drug therapy , Animals , Apoptosis/drug effects , Autophagy/drug effects , Cisplatin/adverse effects , Disease Models, Animal , Disease Progression , Endoplasmic Reticulum/drug effects , Endoplasmic Reticulum/metabolism , Endoplasmic Reticulum/pathology , Humans , Ischemic Preconditioning/methods , Kidney/blood supply , Kidney/drug effects , Molecular Chaperones/administration & dosage , Protein Folding/drug effects , Renal Insufficiency, Chronic/prevention & control , Reperfusion Injury/complications , Thapsigargin/administration & dosage , Treatment Outcome , Tunicamycin/administration & dosage , Unfolded Protein Response/drug effects
4.
PLoS One ; 12(5): e0170792, 2017.
Article in English | MEDLINE | ID: mdl-28493876

ABSTRACT

AIM: Increased levels of circulating sphingosine-1-phosphate (S1P) have been reported in ulcerative colitis. The objective of this study was to examine the effect of S1P on colonic smooth muscle contractility and how is it affected by colitis. METHODS: Colonic inflammation was induced by intrarectal administration of trinitrobenzene sulfonic acid. Five days later colon segments were isolated and used for contractility experiments and immunoblotting. RESULTS: S1P contracted control and inflamed colon segments and the contraction was significantly greater in inflamed colon segments. S1P-induced contraction was mediated by S1PR1 and S1PR2 in control and S1PR2 in inflamed colon segments. S1PR3 did not play a significant role in S1P-induced contractions in control or inflamed colon. S1PR1, S1PR2 and S1PR3 proteins were expressed in colon segments from both groups. The expression of S1PR1 and S1PR2 was significantly enhanced in control and inflamed colon segments, respectively. S1PR3 levels however were not significantly different between the two groups. Nifedipine significantly reduced S1P-induced contraction in control but not inflamed colon segments. Thapsigargin significantly reduced S1P-induced contraction of the inflamed colon. GF 109203X and Y-27632, alone abolished S1P-induced contraction of the control but not inflamed colon segments. Combination of GF 109203X, Y-27632 and thapsigargin abolished S1P-induced contraction of inflamed colon segments. CONCLUSION: S1P contracted control colon via S1PR1 and S1PR2 and inflamed colon exclusively via S1PR2. Calcium influx (control) or release (inflamed) and calcium sensitization are involved in S1P-induced contraction. Exacerbated response to S1P in colitic colon segments may explain altered colonic motility reported in patients and experimental models of inflammatory bowel disease.


Subject(s)
Colitis, Ulcerative/genetics , Inflammation/genetics , Lysophospholipids/genetics , Receptors, Lysosphingolipid/genetics , Sphingosine/analogs & derivatives , Animals , Calcium/metabolism , Colitis, Ulcerative/chemically induced , Colitis, Ulcerative/drug therapy , Colitis, Ulcerative/physiopathology , Colon/drug effects , Colon/physiopathology , Disease Models, Animal , Humans , Inflammation/chemically induced , Inflammation/drug therapy , Inflammation/physiopathology , Muscle Contraction/drug effects , Muscle Contraction/genetics , Muscle, Smooth/metabolism , Muscle, Smooth/physiopathology , Rats , Sphingosine/genetics , Sphingosine-1-Phosphate Receptors , Thapsigargin/administration & dosage , Trinitrobenzenesulfonic Acid/toxicity
5.
Apoptosis ; 22(1): 72-85, 2017 01.
Article in English | MEDLINE | ID: mdl-27778132

ABSTRACT

Endoplasmic reticulum (ER) stress-mediated apoptosis is a well-known factor in the pathogenesis of age-related macular degeneration (AMD). ER stress leads to accumulation of misfolded proteins, which in turn activates unfolded protein response (UPR) of the cell for its survival. The prolonged UPR of ER stress promotes cell death; however, the transition between adaptation and ER stress-induced apoptosis has not been clearly understood. Hence, the present study investigates the regulatory effect of (-)-epigallocatechin gallate (EGCG) on ER stress-induced by hydrogen peroxide (H2O2) and disturbance of calcium homeostasis by thapsigargin (TG) in mouse retinal pigment epithelial (MRPE) cells. The oxidant molecules influenced MRPE cells showed an increased level of intracellular calcium [Ca2+]i in ER and transferred to mitochondria through ER-mitochondrial tether site then increased ROS production. EGCG restores [Ca2+]i homeostasis by decreasing ROS production through inhibition of prohibitin1 which regulate ER-mitochondrial tether site and inhibit apoptosis. Effect of EGCG on ER stress-mediated apoptosis was elucidated by exploring the UPR signalling pathways. EGCG downregulated GRP78, CHOP, PERK, ERO1α, IRE1α, cleaved PARP, cleaved caspase 3, caspase 12 and upregulated expression of calnexinin MRPE cells. In addition to this, inhibition of apoptosis by EGCG was also confirmed with expression of proteins Akt, PTEN and GSK3ß. MRPE cells with EGCG upregulates phosphorylation of Akt at ser473 and phospho ser380 of PTEN, but phosphorylation at ser9 of GSK3ß was inhibited. Further, constitutively active (myristoylated) CA-Akt transfected in MRPE cells had an increased Akt activity in EGCG influenced cells. These findings strongly suggest that antioxidant molecules inhibit cell death through the proper balancing of [Ca2+]i and ROS production in order to maintain UPR of ER in MRPE cells. Thus, modulation of UPR signalling may provide a potential target for the therapeutic approaches of AMD.


Subject(s)
Apoptosis/drug effects , Catechin/analogs & derivatives , Endoplasmic Reticulum Stress/drug effects , Macular Degeneration/drug therapy , Unfolded Protein Response/genetics , Animals , Antioxidants/metabolism , Calcium/metabolism , Calcium Signaling/genetics , Catechin/administration & dosage , Endoplasmic Reticulum Chaperone BiP , Endoplasmic Reticulum Stress/genetics , Humans , Hydrogen Peroxide/toxicity , Macular Degeneration/genetics , Mice , Mitochondria/drug effects , Mitochondria/genetics , Oxidative Stress/drug effects , Reactive Oxygen Species/metabolism , Thapsigargin/administration & dosage , Unfolded Protein Response/drug effects
6.
Theriogenology ; 88: 89-97, 2017 Jan 15.
Article in English | MEDLINE | ID: mdl-27865417

ABSTRACT

The apoptosis of granulosa cells is the main cause of follicular atresia, and endoplasmic reticulum (ER) stress is involved in the apoptosis of granulosa cells. Apoptosis inducing factor (AIF) mediates caspase-independent apoptosis and causes chromatin condensation and DNA fragmentation, but its role in ER stress-mediated granulosa cell apoptosis during goat follicular atresia remains largely unknown. The aim of this study was to investigate the function of AIF in the apoptosis of goat granulosa cells mediated by ER stress. The results of immunohistochemical and Western blot analyses demonstrated that AIF was mainly located in granulosa cells, and the expression of AIF significantly increased during follicular atresia. Then, AIF-short hairpin RNA recombinant lentiviral vectors were constructed successfully and transfected into human telomerase reverse transcriptase-goat granulosa cells (hTERT-GGCs). Real-time quantitative polymerase chain reaction and Western blot analysis confirmed that AIF was effectively knocked down in hTERT-GGCs. Flow cytometry results showed that the knockdown of AIF in hTERT-GGCs reduced apoptosis due to serum starvation or thapsigargin (Tg) treatment. In addition, AIF depletion changed the expression of related molecular marker molecules of ER stress under Tg treatment. In conclusion, AIF may serve as a key factor during follicular atresia, and AIF depletion protects ER stress-mediated goat granulosa cell apoptosis.


Subject(s)
Apoptosis Inducing Factor/metabolism , Endoplasmic Reticulum/physiology , Goats , Granulosa Cells/physiology , Animals , Cell Proliferation , Cell Survival , Enzyme Inhibitors/administration & dosage , Enzyme Inhibitors/pharmacology , Female , Gene Expression Regulation/drug effects , Gene Expression Regulation/physiology , Gene Knockdown Techniques , Stress, Physiological , Thapsigargin/administration & dosage , Thapsigargin/pharmacology
7.
Sci Rep ; 6: 38832, 2016 12 13.
Article in English | MEDLINE | ID: mdl-27958308

ABSTRACT

Radiation-induced bystander effect (RIBE) has important implications for secondary cancer risk assessment during cancer radiotherapy, but the defense and self-protective mechanisms of bystander normal cells are still largely unclear. The present study found that micronuclei (MN) formation could be induced in the non-irradiated HL-7702 hepatocyte cells after being treated with the conditioned medium from irradiated hepatoma HepG2 cells under either normoxia or hypoxia, where the ratio of the yield of bystander MN induction to the yield of radiation-induced MN formation under hypoxia was much higher than that of normoxia. Nonetheless, thapsigargin induced endoplasmic reticulum (ER) stress and dramatically suppressed this bystander response manifested as the decrease of MN and apoptosis inductions. Meanwhile, the interference of BiP gene, a major ER chaperone, amplified the detrimental RIBE. More precisely, thapsigargin provoked ER sensor of PERK to initiate an instantaneous and moderate ER stress thus defensed the hazard form RIBE, while BiP depletion lead to persistently destroyed homeostasis of ER and exacerbated cell injury. These findings provide new insights that the mild ER stress through BiP-PERK-p-eIF2α signaling pathway has a profound role in protecting cellular damage from RIBE and hence may decrease the potential secondary cancer risk after cancer radiotherapy.


Subject(s)
Bystander Effect/radiation effects , Endoplasmic Reticulum Stress , Hepatocytes/radiation effects , Activating Transcription Factor 6/metabolism , Apoptosis/radiation effects , Cell Hypoxia , Culture Media, Conditioned , Endoplasmic Reticulum Chaperone BiP , Enzyme Inhibitors/administration & dosage , Eukaryotic Initiation Factor-2/metabolism , Heat-Shock Proteins/metabolism , Hep G2 Cells , Hepatocytes/physiology , Humans , Signal Transduction , Thapsigargin/administration & dosage , X-Box Binding Protein 1 , eIF-2 Kinase/metabolism
8.
Sci Rep ; 6: 35196, 2016 10 12.
Article in English | MEDLINE | ID: mdl-27731378

ABSTRACT

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent for esophageal squamous cell carcinoma (ESCC). Forced expression of CHOP, one of the key downstream transcription factors during endoplasmic reticulum (ER) stress, upregulates the death receptor 5 (DR5) levels and promotes oxidative stress and cell death. In this study, we show that ER stress mediated by thapsigargin promoted CHOP and DR5 synthesis thus sensitizing TRAIL treatment, which induced ESCC cells apoptosis. These effects were reversed by DR5 siRNA in vitro and CHOP siRNA both in vitro and in vivo. Besides, chemically inhibition of AMPK by Compound C and AMPK siRNA weakened the anti-cancer effect of thapsigargin and TRAIL co-treatment. Therefore, our findings suggest ER stress effectively sensitizes human ESCC to TRAIL-mediated apoptosis via the TRAIL-DR5-AMPK signaling pathway, and that activation of ER stress may be beneficial for improving the efficacy of TRAIL-based anti-cancer therapy.


Subject(s)
AMP-Activated Protein Kinases/metabolism , Apoptosis/drug effects , Carcinoma, Squamous Cell/drug therapy , Esophageal Neoplasms/drug therapy , TNF-Related Apoptosis-Inducing Ligand/administration & dosage , Thapsigargin/administration & dosage , Antineoplastic Agents/administration & dosage , Carcinoma, Squamous Cell/metabolism , Carcinoma, Squamous Cell/pathology , Cell Adhesion/drug effects , Cell Line, Tumor , Cell Movement/drug effects , Cell Survival/drug effects , Drug Synergism , Drug Therapy, Combination , Endoplasmic Reticulum Stress/drug effects , Enzyme Activation/drug effects , Esophageal Neoplasms/metabolism , Esophageal Neoplasms/pathology , Esophageal Squamous Cell Carcinoma , Humans , Neoplasm Invasiveness/prevention & control , RNA, Small Interfering/genetics , Reactive Oxygen Species/metabolism , Receptors, TNF-Related Apoptosis-Inducing Ligand/antagonists & inhibitors , Receptors, TNF-Related Apoptosis-Inducing Ligand/genetics , Receptors, TNF-Related Apoptosis-Inducing Ligand/metabolism , Transcription Factor CHOP/antagonists & inhibitors , Transcription Factor CHOP/genetics , Transcription Factor CHOP/metabolism , Up-Regulation/drug effects
9.
Br J Cancer ; 114(9): 986-94, 2016 Apr 26.
Article in English | MEDLINE | ID: mdl-27115568

ABSTRACT

BACKGROUND: Mipsagargin (G-202; (8-O-(12-aminododecanoyl)-8-O-debutanoyl thapsigargin)-Asp-γ-Glu-γ-Glu-γ-GluGluOH)) is a novel thapsigargin-based targeted prodrug that is activated by PSMA-mediated cleavage of an inert masking peptide. The active moiety is an inhibitor of the sarcoplasmic/endoplasmic reticulum calcium adenosine triphosphatase (SERCA) pump protein that is necessary for cellular viability. We evaluated the safety of mipsagargin in patients with advanced solid tumours and established a recommended phase II dosing (RP2D) regimen. METHODS: Patients with advanced solid tumours received mipsagargin by intravenous infusion on days 1, 2 and 3 of 28-day cycles and were allowed to continue participation in the absence of disease progression or unacceptable toxicity. The dosing began at 1.2 mg m(-2) and was escalated using a modified Fibonacci schema to determine maximally tolerated dose (MTD) with an expansion cohort at the RP2D. Plasma was analysed for mipsagargin pharmacokinetics and response was assessed using RECIST criteria. RESULTS: A total of 44 patients were treated at doses ranging from 1.2 to 88 mg m(-2), including 28 patients in the dose escalation phase and 16 patients in an expansion cohort. One dose-limiting toxicity (DLT; Grade 3 rash) was observed in the dose escalation portion of the study. At 88 mg m(-2), observations of Grade 2 infusion-related reaction (IRR, 2 patients) and Grade 2 creatinine elevation (1 patient) led to declaration of 66.8 mg m(-2) as the recommended phase II dose (RP2D). Across the study, the most common treatment-related adverse events (AEs) were fatigue, rash, nausea, pyrexia and IRR. Two patients developed treatment-related Grade 3 acute renal failure that was reversible during the treatment-free portion of the cycle. To help ameliorate the IRR and creatinine elevations, a RP2D of 40 mg m(-2) on day 1 and 66.8 mg m(-2) on days 2 and 3 with prophylactic premedications and hydration on each day of infusion was established. Clinical response was not observed, but prolonged disease stabilisation was observed in a subset of patients. CONCLUSIONS: Mipsagargin demonstrated an acceptable tolerability and favourable pharmacokinetic profile in patients with solid tumours.


Subject(s)
Enzyme Inhibitors/therapeutic use , Neoplasms/drug therapy , Prodrugs/therapeutic use , Thapsigargin/therapeutic use , Aged , Aged, 80 and over , Enzyme Inhibitors/administration & dosage , Enzyme Inhibitors/pharmacokinetics , Humans , Male , Middle Aged , Thapsigargin/administration & dosage , Thapsigargin/pharmacokinetics
10.
Mol Cancer Res ; 14(4): 397-407, 2016 Apr.
Article in English | MEDLINE | ID: mdl-26869290

ABSTRACT

UNLABELLED: To assess the role of the serum and glucocorticoid-regulated kinase (SGK) kinase in multiple myeloma, we ectopically expressed wild type or a phosphomimetic version of SGK into multiple myeloma cell lines. These cells were specifically resistant to the ER stress inducers tunicamycin, thapsigargin, and bortezomib. In contrast, there was no alteration of sensitivity to dexamethasone, serum starvation, or mTORC inhibitors. Mining of genomic data from a public database indicated that low baseline SGK expression in multiple myeloma patients correlated with enhanced ability to undergo a complete response to subsequent bortezomib treatment and a longer time to progression and overall survival following treatment. SGK overexpressing multiple myeloma cells were also relatively resistant to bortezomib in a murine xenograft model. Parental/control multiple myeloma cells demonstrated a rapid upregulation of SGK expression and activity (phosphorylation of NDRG-1) during exposure to bortezomib and an SGK inhibitor significantly enhanced bortezomib-induced apoptosis in cell lines and primary multiple myeloma cells. In addition, a multiple myeloma cell line selected for bortezomib resistance demonstrated enhanced SGK expression and SGK activity. Mechanistically, SGK overexpression constrained an ER stress-induced JNK proapoptotic pathway and experiments with a SEK mutant supported the notion that SGK's protection against bortezomib was mediated via its phosphorylation of SEK (MAP2K4) which abated SEK/JNK signaling. These data support a role for SGK inhibitors in the clinical setting for myeloma patients receiving treatment with ER stress inducers like bortezomib. IMPLICATIONS: Enhanced SGK expression and activity in multiple myeloma cells contributes to resistance to ER stress, including bortezomib challenge.


Subject(s)
Bortezomib/administration & dosage , Drug Resistance, Neoplasm , Immediate-Early Proteins/genetics , Immediate-Early Proteins/metabolism , Multiple Myeloma/drug therapy , Protein Serine-Threonine Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , Animals , Apoptosis , Bortezomib/pharmacology , Cell Line, Tumor , Endoplasmic Reticulum Stress , Female , Gene Expression Regulation, Neoplastic , Humans , Mice , Multiple Myeloma/genetics , Multiple Myeloma/metabolism , Thapsigargin/administration & dosage , Thapsigargin/pharmacology , Tunicamycin/administration & dosage , Tunicamycin/pharmacology , Up-Regulation , Xenograft Model Antitumor Assays
11.
Neuropharmacology ; 103: 195-210, 2016 Apr.
Article in English | MEDLINE | ID: mdl-26631534

ABSTRACT

Cytoplasmic calcium (Ca(2+)) mobilization has been proposed to be an important factor in the induction of emesis. The selective sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase (SERCA) inhibitor thapsigargin, is known to deplete intracellular Ca(2+) stores, which consequently evokes extracellular Ca(2+) entry through cell membrane-associated channels, accompanied by a prominent rise in cytosolic Ca(2+). A pro-drug form of thapsigargin is currently under clinical trial as a targeted cancer chemotherapeutic. We envisioned that the intracellular effects of thapsigargin could cause emesis and planned to investigate its mechanisms of emetic action. Indeed, thapsigargin did induce vomiting in the least shrew in a dose-dependent and bell-shaped manner, with maximal efficacy (100%) at 0.5 mg/kg (i.p.). Thapsigargin (0.5 mg/kg) also caused increases in c-Fos immunoreactivity in the brainstem emetic nuclei including the area postrema (AP), nucleus tractus solitarius (NTS) and dorsal motor nucleus of the vagus (DMNX), as well as enhancement of substance P (SP) immunoreactivity in DMNX. In addition, thapsigargin (0.5 mg/kg, i.p.) led to vomit-associated and time-dependent increases in phosphorylation of Ca(2+)/calmodulin kinase IIα (CaMKIIα) and extracellular signal-regulated protein kinase 1/2 (ERK1/2) in the brainstem. We then explored the suppressive potential of diverse chemicals against thapsigargin-evoked emesis including antagonists of: i) neurokinin-1 receptors (netupitant), ii) the type 3 serotonin receptors (palonosetron), iii) store-operated Ca(2+) entry (YM-58483), iv) L-type Ca(2+) channels (nifedipine), and v) SER Ca(2+)-release channels inositol trisphosphate (IP3Rs) (2-APB)-, and ryanodine (RyRs) (dantrolene)-receptors. In addition, the antiemetic potential of inhibitors of CaMKII (KN93) and ERK1/2 (PD98059) were investigated. All tested antagonists/blockers attenuated emetic parameters to varying degrees except palonosetron, however a combination of non-effective doses of netupitant and palonosetron exhibited additive antiemetic efficacy. A low-dose combination of nifedipine and 2-APB plus dantrolene mixture completely abolished thapsigargin-evoked vomiting, CaMKII-ERK1/2 activation and SP elevation. In addition, pretreatment with KN93 or PD98059 suppressed thapsigargin-induced increases in SP and ERK1/2 activation. Intracerebroventricular injection of netupitant suppressed vomiting caused by thapsigargin which suggests that the principal site of evoked emesis is the brainstem. In sum, this is the first study to demonstrate that thapsigargin causes vomiting via the activation of the Ca(2+)-CaMKII-ERK1/2 cascade, which is associated with an increase in the brainstem tissue content of SP, and the evoked emesis occurs through SP-induced activation of neurokinin-1 receptors.


Subject(s)
Brain Stem/metabolism , Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism , MAP Kinase Signaling System , Substance P/metabolism , Thapsigargin/administration & dosage , Vomiting/chemically induced , Vomiting/metabolism , Animals , Calcium/metabolism , Female , Male , Phosphorylation , Receptors, Neurokinin-1/metabolism , Shrews
12.
PLoS One ; 10(9): e0137359, 2015.
Article in English | MEDLINE | ID: mdl-26352986

ABSTRACT

BACKGROUND: Atrial fibrillation (AF) is the most common arrhythmia in humans, yet; treatment has remained sub-optimal due to poor understanding of the underlying mechanisms. Cardiac alternans precede AF episodes, suggesting an important arrhythmia substrate. Recently, we demonstrated ventricular SERCA2a overexpression suppresses cardiac alternans and arrhythmias. Therefore, we hypothesized that atrial SERCA2a overexpression will decrease cardiac alternans and arrhythmias. METHODS: Adult rat isolated atrial myocytes where divided into three treatment groups 1) Control, 2) SERCA2a overexpression (Ad.SERCA2a) and 3) SERCA2a inhibition (Thapsigargin, 1µm). Intracellular Ca2+ was measured using Indo-1AM and Ca2+ alternans (Ca-ALT) was induced with a standard ramp pacing protocol. RESULTS: As predicted, SR Ca2+ reuptake was enhanced with SERCA2a overexpression (p< 0.05) and reduced with SERCA2a inhibition (p<0.05). Surprisingly, there was no difference in susceptibility to Ca-ALT with either SERCA2a overexpression or inhibition when compared to controls (p = 0.73). In contrast, SERCA2a overexpression resulted in increased premature SR Ca2+ (SCR) release compared to control myocytes (28% and 0%, p < 0.05) and concomitant increase in SR Ca2+ load (p<0.05). Based on these observations we tested in-vivo atrial arrhythmia inducibility in control and Ad.SERCA2a animals using an esophageal atrial burst pacing protocol. There were no inducible atrial arrhythmias in Ad.GFP (n = 4) animals though 20% of Ad.SERCA2a (n = 5) animals had inducible atrial arrhythmias (p = 0.20). CONCLUSIONS: Our findings suggest that unlike the ventricle, SERCA2a is not a key regulator of cardiac alternans in the atrium. Importantly, SERCA2a overexpression in atrial myocytes can increase SCR, which may be arrhythmogenic.


Subject(s)
Arrhythmias, Cardiac/genetics , Atrial Fibrillation/genetics , Calcium/metabolism , Sarcoplasmic Reticulum Calcium-Transporting ATPases/biosynthesis , Animals , Arrhythmias, Cardiac/metabolism , Arrhythmias, Cardiac/pathology , Atrial Fibrillation/metabolism , Atrial Fibrillation/pathology , Brugada Syndrome , Cardiac Conduction System Disease , Gene Expression Regulation , Glucans/metabolism , Heart Atria/metabolism , Heart Atria/pathology , Heart Conduction System/abnormalities , Heart Conduction System/metabolism , Heart Conduction System/pathology , Humans , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/metabolism , Myocytes, Cardiac/pathology , Patch-Clamp Techniques , Rats , Sarcoplasmic Reticulum Calcium-Transporting ATPases/antagonists & inhibitors , Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism , Thapsigargin/administration & dosage
13.
J Biol Chem ; 290(2): 1086-95, 2015 Jan 09.
Article in English | MEDLINE | ID: mdl-25451935

ABSTRACT

The organellar targeting of two-pore channels (TPCs) and their capacity to associate as homo- and heterodimers may be critical to endolysosomal signaling. A more detailed understanding of the functional association of vertebrate TPC1-3 is therefore necessary. We report here that when stably expressed in HEK293 cells, human (h) TPC1 and chicken (c) TPC3 were specifically targeted to different subpopulations of endosomes, hTPC2 was specifically targeted to lysosomes, and rabbit (r) TPC3 was specifically targeted to both endosomes and lysosomes. Intracellular dialysis of NAADP evoked a Ca(2+) transient in HEK293 cells that stably overexpressed hTPC1, hTPC2, and rTPC3, but not in cells that stably expressed cTPC3. The Ca(2+) transients induced in cells that overexpressed endosome-targeted hTPC1 were abolished upon depletion of acidic Ca(2+) stores by bafilomycin A1, but remained unaffected following depletion of endoplasmic reticulum stores by thapsigargin. In contrast, Ca(2+) transients induced via lysosome-targeted hTPC2 and endolysosome-targeted rTPC3 were abolished by bafilomycin A1 and markedly attenuated by thapsigargin. NAADP induced marked Ca(2+) transients in HEK293 cells that stably coexpressed hTPC2 with hTPC1 or cTPC3, but failed to evoke any such response in cells that coexpressed interacting hTPC2 and rTPC3 subunits. We therefore conclude that 1) all three TPC subtypes may support Ca(2+) signaling from their designate acidic stores, and 2) lysosome-targeted (but not endosome-targeted) TPCs support coupling to the endoplasmic reticulum.


Subject(s)
Calcium Channels/metabolism , Calcium Signaling/drug effects , Endosomes/metabolism , Animals , Calcium/metabolism , Calcium Channels/biosynthesis , Calcium Channels/chemistry , Chickens , Endoplasmic Reticulum/drug effects , Endoplasmic Reticulum/metabolism , Endosomes/drug effects , Gene Expression Regulation/drug effects , HEK293 Cells , Humans , Lysosomes/drug effects , Lysosomes/metabolism , Macrolides/administration & dosage , NADP/administration & dosage , NADP/analogs & derivatives , Rabbits , Thapsigargin/administration & dosage
14.
Mol Cancer ; 13: 148, 2014 Jun 12.
Article in English | MEDLINE | ID: mdl-24924916

ABSTRACT

BACKGROUND: Resistance to cell death in the presence of stressful stimuli is one of the hallmarks of cancer cells acquired during multistep tumorigenesis, and knowledge of the molecular mechanism of stress adaptation can be exploited to develop cancer-selective therapeutics. Mitochondria and the endoplasmic reticulum (ER) are physically interconnected organelles that can sense and exchange various stress signals. Although there have been many studies on stress propagation from the ER to mitochondria, reverse stress signals originating from mitochondria have not been well reported. METHODS: After inactivation of the proteins by pharmacologic and genetic methods, the signal pathways were analyzed by fluorescence microscopy, flow cytometry, MTT assay, and western blotting. A mouse xenograft model was used to examine synergistic anticancer activity and the action mechanism of drugs in vivo. RESULTS: We show in this study that mitochondrial heat shock protein 90 (Hsp90) suppresses mitochondria-initiated calcium-mediated stress signals propagating into the ER in cancer cells. Mitochondrial Hsp90 inhibition triggers the calcium signal by opening the mitochondrial permeability transition pore and, in turn, the ER ryanodine receptor, via calcium-induced calcium release. Subsequent depletion of ER calcium activates unfolded protein responses in the ER lumen, thereby increasing the expression of a pro-apoptotic transcription factor, CEBP homologous protein (CHOP). Combined treatment with the ER stressor thapsigargin and the mitochondrial Hsp90 inhibitor gamitrinib augmented interorganelle stress signaling by elevating CHOP expression, and showed synergistic cytotoxic activity exclusively in cancer cells in vitro and in vivo. CONCLUSIONS: Collectively, mitochondrial Hsp90s confer cell death resistance to cancer cells by suppressing the mitochondria-initiated calcium-mediated interorganelle stress response.


Subject(s)
Calcium Signaling , Endoplasmic Reticulum Stress , HSP90 Heat-Shock Proteins/genetics , Transcription Factor CHOP/biosynthesis , Animals , Calcium/metabolism , Cell Line, Tumor , Endoplasmic Reticulum/genetics , Endoplasmic Reticulum/metabolism , Gene Expression Regulation, Neoplastic , Guanidines/administration & dosage , HSP90 Heat-Shock Proteins/metabolism , Humans , Lactams, Macrocyclic/administration & dosage , Mice , Mitochondria/metabolism , Mitochondria/pathology , Thapsigargin/administration & dosage , Transcription Factor CHOP/genetics
15.
ScientificWorldJournal ; 2014: 605416, 2014.
Article in English | MEDLINE | ID: mdl-24688409

ABSTRACT

A series of experiments have been carried out to investigate the effects of different concentrations of thapsigargin (0, 0.001, 0.1, and 1 µM) on the proliferation and survival of human rheumatoid arthritis synovial cells (MH7A). The results showed that thapsigargin can block the cell proliferation in human rheumatoid arthritis synovial cells in a time- and dose-dependent manner. Results of Hoechst staining suggested that thapsigargin may induce cell apoptosis in MH7A cells in a time- and dose-dependent manner, and the percentages of cell death reached 44.6% at thapsigargin concentration of 1 µM treated for 4 days compared to the control. The protein and mRNA levels of cyclin D1 decreased gradually with the increasing of thapsigargin concentration and treatment times. Moreover, the protein levels of mTORC1 downstream indicators pS6K and p4EBP-1 were reduced by thapsigargin treatment at different concentrations and times, which should be responsible for the reduced cyclin D1 expressions. Our results revealed that thapsigargin may effectively impair the cell proliferation and survival of MH7A cells. The present findings will help to understand the molecular mechanism of fibroblast-like synoviocytes proliferations and suggest that thapsigargin is of potential for the clinical treatment of rheumatoid arthritis.


Subject(s)
Arthritis, Rheumatoid/pathology , Cell Proliferation/drug effects , Cell Survival/drug effects , Synovial Fluid/cytology , Synovial Fluid/drug effects , Thapsigargin/administration & dosage , Cell Line , Dose-Response Relationship, Drug , Humans
16.
Life Sci ; 92(1): 72-80, 2013 Jan 17.
Article in English | MEDLINE | ID: mdl-23154241

ABSTRACT

AIMS: Endoplasmic reticulum (ER) stress modulates gene expression and has been implicated in causing dyslipidemias. To determine if ER stress may contribute to hypoalphalipoproteinemia through suppression of apo A-I gene expression, human hepatoma cell line Hep G2 was treated with ER stress inducers and the changes in apo A-I gene expression were compared to albumin gene expression. MAIN METHODS: HepG2 cells were treated with tunicamycin (TM) and thapsigargin (TG), two potent inducers of ER stress, and apo A-I and albumin protein levels, mRNA levels, and promoter activity were measured. ER stress was measured using the ER stress-responsive alkaline phosphatase assay and by Western blot quantitation of ER stress markers such as glucose-regulated protein-78 (GRP-78), phosphorylated Jun N-terminal kinase (phospho-JNK), total JNK, phosphorylated eukaryotic initiation factor 2 alpha (phospho eIF2α), and total eIF2α. KEY FINDINGS: TM and TG induced ER stress in HepG2 cells and reduced apo A-I and albumin secretion in a dose-dependent manner. Intracellular albumin levels increased in cells treated with TM and TG while intracellular apo A-I levels decreased. Albumin mRNA and albumin gene promoter activity were reduced in proportion to the decrease in albumin secreted while changes in the apo A-I mRNA levels and promoter activity were modest and did not account for the reduction in apo A-I secretion. SIGNIFICANCE: These results indicate that apo A-I secretion is inhibited by ER stress possibly by affecting cellular degradation pathways. However, ER stress does not affect apo A-I secretion by regulating gene expression.


Subject(s)
Apolipoprotein A-I/metabolism , Endoplasmic Reticulum Stress/drug effects , Gene Expression Regulation/drug effects , Thapsigargin/pharmacology , Tunicamycin/pharmacology , Albumins/genetics , Albumins/metabolism , Apolipoprotein A-I/genetics , Biomarkers/metabolism , Blotting, Western , Dose-Response Relationship, Drug , Hep G2 Cells , Humans , Promoter Regions, Genetic/genetics , RNA, Messenger/metabolism , Thapsigargin/administration & dosage , Tunicamycin/administration & dosage
17.
Mol Cell Biochem ; 364(1-2): 59-70, 2012 May.
Article in English | MEDLINE | ID: mdl-22270541

ABSTRACT

Accumulation of misfolded proteins and alterations in calcium homeostasis induces endoplasmic reticulum (ER) stress, leading to apoptosis. In this study, we tested the hypothesis that ß-AR stimulation induces ER stress, and induction of ER stress plays a pro-apoptotic role in cardiac myocytes. Using thapsigargin and brefeldin A, we demonstrate that ER stress induces apoptosis in adult rat ventricular myocytes (ARVMs). ß-AR-stimulation (isoproterenol; 3h) significantly increased expression of ER stress proteins, such as GRP-78, Gadd-153, and Gadd-34, while activating caspase-12 in ARVMs. In most parts, these effects were mimicked by thapsigargin. ß-AR stimulation for 15 min increased PERK and eIF-2α phosphorylation. PERK phosphorylation remained higher, while eIF-2α phosphorylation declined thereafter, reaching to ~50% below basal levels at 3 h after ß-AR stimulation. This decline in eIF-2α phosphorylation was prevented by ß1-AR, not by ß2-AR antagonist. Forskolin, adenylyl cyclase activator, simulated the effects of ISO on eIF-2α phosphorylation. Salubrinal (SAL), an ER stress inhibitor, maintained eIF-2α phosphorylation and inhibited ß-AR-stimulated apoptosis. Furthermore, inhibition of caspase-12 using z-ATAD inhibited ß-AR-stimulated and thapsigargin-induced apoptosis. In vivo, ß-AR stimulation induced ER stress in the mouse heart as evidenced by increased expression of GRP-78 and Gadd-153, activation of caspase-12, and dephosphorylation of eIF-2α. SAL maintained phosphorylation of eIF-2α, inhibited activation of caspase-12, and decreased ß-AR-stimulated apoptosis in the heart. Thus, ß-AR stimulation induces ER stress in cardiac myocytes and in the heart, and induction of ER stress plays a pro-apoptotic role.


Subject(s)
Antigens, Differentiation/metabolism , Apoptosis/drug effects , Endoplasmic Reticulum Stress/drug effects , Gene Expression Regulation/drug effects , Isoproterenol/administration & dosage , Proto-Oncogene Proteins/metabolism , Receptors, Adrenergic, beta-1/metabolism , Receptors, Adrenergic, beta-2/metabolism , Transcription Factor CHOP/metabolism , Adenylyl Cyclases/metabolism , Adrenergic beta-1 Receptor Antagonists/pharmacology , Adrenergic beta-2 Receptor Antagonists/pharmacology , Animals , Antigens, Differentiation/genetics , Apoptosis/genetics , Brefeldin A/administration & dosage , Caspase 12/metabolism , Caspase Inhibitors , Cells, Cultured , Cinnamates/pharmacology , Colforsin/pharmacology , Eukaryotic Initiation Factor-2/metabolism , Heat-Shock Proteins/metabolism , Male , Mice , Myocytes, Cardiac/drug effects , Phosphorylation/drug effects , Proto-Oncogene Proteins/genetics , Rats , Rats, Sprague-Dawley , Receptors, Adrenergic, beta-1/genetics , Receptors, Adrenergic, beta-2/genetics , Signal Transduction , Thapsigargin/administration & dosage , Thiourea/analogs & derivatives , Thiourea/pharmacology , Transcription Factor CHOP/genetics , eIF-2 Kinase/metabolism
18.
Toxicol Appl Pharmacol ; 258(2): 208-15, 2012 Jan 15.
Article in English | MEDLINE | ID: mdl-22108590

ABSTRACT

The neuroblastoma-spinal motor neuron fusion cell line, NSC-34, in its differentiated form, NSC-34D, permits examining the effects of riluzole, a proven treatment for amyotrophic lateral sclerosis (ALS) on cell death induction by staurosporine (STS), thapsigargin (Thaps), hydrogen peroxide (H(2)O(2)) and homocysteine (HCy). These neurotoxins, applied exogenously, have mechanisms of action related to the various proposed molecular pathogenetic pathways in ALS and are differentiated from endogenous cell death that is associated with cytoplasmic aggregate formation in motor neurons. Nuclear morphology, caspase-3/7 activation and high content imaging were used to assess toxicity of these neurotoxins with and without co-treatment with riluzole, a benzothiazole compound with multiple pharmacological actions. STS was the most potent neurotoxin at killing NSC-34D cells with a toxic concentration at which 50% of maximal cell death is achieved (TC(50)=0.01µM), followed by Thaps (TC(50)=0.9µM) and H(2)O(2) (TC(50)=15µM) with HCy requiring higher concentrations to kill at the same level (TC(50)=2200µM). Riluzole provided neurorescue with a 20% absolute reduction (47.6% relative reduction) in apoptotic cell death against Thaps-induced NSC-34D cell (p≤0.05), but had no effect on STS-, H(2)O(2)- and HCy-induced NSC-34D cell death. This effect of riluzole on Thaps induction of cell death was independent of caspase-3/7 activation. Riluzole mitigated a toxin that can cause intracellular calcium dysregulation associated with endoplasmic reticulum (ER) stress but not toxins associated with other cell death mechanisms.


Subject(s)
Amyotrophic Lateral Sclerosis/drug therapy , Apoptosis/drug effects , Neuroprotective Agents/pharmacology , Neurotoxins/toxicity , Riluzole/pharmacology , Amyotrophic Lateral Sclerosis/physiopathology , Animals , Calcium/metabolism , Caspase 3/metabolism , Caspase 7/metabolism , Cell Line , Dose-Response Relationship, Drug , Endoplasmic Reticulum/metabolism , Homocysteine/administration & dosage , Homocysteine/toxicity , Hybrid Cells , Hydrogen Peroxide/administration & dosage , Hydrogen Peroxide/toxicity , Mice , Motor Neurons/drug effects , Motor Neurons/metabolism , Neuroblastoma/metabolism , Neurotoxins/administration & dosage , Staurosporine/administration & dosage , Staurosporine/toxicity , Thapsigargin/administration & dosage , Thapsigargin/toxicity
19.
J Cardiovasc Pharmacol ; 57(4): 434-8, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21242807

ABSTRACT

We investigated the effects of endoplasmic reticulum (ER) stress inducers thapsigargin (TG) and tunicamycin (Tm) on immunostimulant lipopolysaccharide/interferon (LPS/IFN)-induced expression of isoform of nitric oxide synthase (iNOS) and nitric oxide (NO) production in vascular smooth muscle cells. LPS/IFN-induced iNOS mRNA expression was markedly enhanced by TG, whereas iNOS mRNA expression was strongly attenuated by Tm. Similarly, production of iNOS protein was markedly upregulated by TG but virtually eliminated by Tm. LPS/IFN-induced guanosine triphosphate cyclohydrolase I mRNA expression was slightly reduced by TG and markedly inhibited by Tm. Similarly, LPS/IFN-mediated induction of cellular biopterin was modestly reduced by TG and markedly inhibited by Tm. TG modestly enhanced LPS/IFN-induced activation of NF-κB, whereas Tm had no effect on it. Cellular respiration was reduced by TG and Tm in a concentration-dependent manner, which was confirmed by apoptosis assay. Thus, TG and Tm-induced ER stress and differently modulated NO production through alterations in iNOS expression and activity independently of NF-κB activation and caused a similar degree of ER stress-induced apoptosis.


Subject(s)
Endoplasmic Reticulum/drug effects , Nitric Oxide/biosynthesis , Thapsigargin/pharmacology , Tunicamycin/pharmacology , Animals , Cell Respiration/drug effects , Dose-Response Relationship, Drug , Endoplasmic Reticulum/metabolism , GTP Cyclohydrolase/drug effects , GTP Cyclohydrolase/genetics , GTP Cyclohydrolase/metabolism , Gene Expression Regulation, Enzymologic/drug effects , Interferon-gamma/pharmacology , Lipopolysaccharides/pharmacology , Male , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/drug effects , Muscle, Smooth, Vascular/metabolism , Nitric Oxide Synthase Type II/drug effects , Nitric Oxide Synthase Type II/genetics , Nitric Oxide Synthase Type II/metabolism , RNA, Messenger/metabolism , Rats , Rats, Wistar , Thapsigargin/administration & dosage , Tunicamycin/administration & dosage
20.
Biophys J ; 96(11): 4514-24, 2009 Jun 03.
Article in English | MEDLINE | ID: mdl-19486674

ABSTRACT

The plasma membrane electrical activities of neurons that secrete gonadotropin-releasing hormone (GnRH) have been studied extensively. A couple of mathematical models have been developed previously to explain different aspects of these activities. The goal of this article is to develop a single model that accounts for the previously modeled experimental results and some more recent results that have not been accounted for. The latter includes two types of membrane potential bursting mechanisms and their associated cytosolic calcium oscillations. One bursting mechanism has not been reported in experiments and is thus regarded as a model prediction. Although the model is mainly based on data collected in immortalized GnRH cell lines, it is capable of explaining some properties of GnRH neurons observed in several other preparations including mature GnRH neurons in hypothalamic slices. We present a spatial model that incorporates a detailed description of calcium dynamics in a three-dimensional cell body with the ion channels evenly distributed on the cell surface. A phenomenological reduction of the spatial model into a simplified form is also presented. The simplified model will facilitate the study of the roles of plasma membrane electrical activities in the pulsatile release of GnRH.


Subject(s)
Calcium/metabolism , Gonadotropin-Releasing Hormone/metabolism , Membrane Potentials/physiology , Models, Neurological , Neurons/physiology , Action Potentials/drug effects , Action Potentials/physiology , Algorithms , Apamin/administration & dosage , Colforsin/administration & dosage , Cyclic AMP/administration & dosage , Enzyme Inhibitors/administration & dosage , Membrane Potentials/drug effects , Neurons/drug effects , Neurotoxins/administration & dosage , Sodium/metabolism , Thapsigargin/administration & dosage
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