ABSTRACT
BACKGROUND: The detection rate of human papillomavirus (HPV) in Bowen's disease (BD) varies greatly. OBJECTIVES: To detect HPV DNA in BD samples using next-generation sequencing (NGS) and compare HPV detection rates between pelvic and non-pelvic BD. MATERIALS AND METHODS: We evaluated 99 patients with BD in our institution. DNA was extracted from formalin-fixed and paraffin embedded (FFPE) tissue blocks. The presence of HPV DNA material was detected using special kit-based NGS technology. Clinical characteristics and HPV detection rates were then compared between pelvic and non-pelvic BD samples. RESULTS: HPV was detected in 26 (26.3%) BD samples. A total of 10 types of α-HPV was detected: HPV 16, 53, 31, 58, 66, 26, 27, 57, 45, and 72. The most common HPV type was 16 (12.1%). Only two types (27 and 57) were frequently classified as cutaneous type, and the rest were mucosal types. The HPV detection rate was significantly higher in pelvic BD (45.2%) compared to non-pelvic BD (17.6%). CONCLUSION: The present study suggests that sexually transmitted mucosal α-HPV plays a significant role in the pathogenesis of BD, especially in the pelvic region.
Subject(s)
Alphapapillomavirus/isolation & purification , Bowen's Disease/virology , DNA, Viral/isolation & purification , Papillomavirus Infections/virology , Sexually Transmitted Diseases, Viral/virology , Skin/virology , Abdominal Wall/pathology , Abdominal Wall/virology , Aged , Aged, 80 and over , Alphapapillomavirus/genetics , Biopsy , Bowen's Disease/etiology , Buttocks , DNA, Viral/genetics , Female , Genotype , Genotyping Techniques , Groin/pathology , Groin/virology , High-Throughput Nucleotide Sequencing , Human papillomavirus 16/genetics , Human papillomavirus 16/isolation & purification , Humans , Male , Middle Aged , Mucous Membrane/pathology , Mucous Membrane/virology , Papillomavirus Infections/complications , Papillomavirus Infections/genetics , Retrospective Studies , Sexually Transmitted Diseases, Viral/complications , Skin/pathology , Thigh/pathology , Thigh/virologySubject(s)
Herpes Zoster/diagnosis , Neck/pathology , Neck/virology , Thigh/pathology , Thigh/virology , Aged, 80 and over , Female , HumansSubject(s)
Cytomegalovirus Infections/virology , Fever/complications , HIV Infections/complications , Lymphoma, AIDS-Related/drug therapy , Neutropenia/complications , Antineoplastic Combined Chemotherapy Protocols , Antiviral Agents/therapeutic use , Cyclophosphamide , Cytomegalovirus Infections/diagnosis , Cytomegalovirus Infections/drug therapy , Doxorubicin , Fever/blood , Fever/diagnosis , Foscarnet/therapeutic use , HIV Infections/blood , Humans , Immunohistochemistry , Lymphoma, AIDS-Related/blood , Lymphoma, AIDS-Related/complications , Lymphoma, AIDS-Related/virology , Male , Middle Aged , Necrosis/pathology , Necrosis/virology , Neutropenia/diagnosis , Prednisone , Skin/pathology , Skin/virology , Thigh/pathology , Thigh/virology , VincristineABSTRACT
Latency within the nervous system is a characteristic feature of herpesviridae infection. It is reactivated by triggering factors such as UV exposure, stress, and trauma. Simultaneous reactivation of herpes simplex and herpes zoster is uncommon, however, an observation provably explained by differences in the triggering mechanism. Concurrent reactivation of herpes simplex virus (HSV) and varicella zoster virus (VZV) is occasionally encountered in immunosuppressed patients; on the other hand, it is rarely reported in immunocompetent individuals. We present the case of an immunocompetent 8-yr-old female patient with concurrent reactivation of HSV on the face and VZV on the right L2 dermatome.
Subject(s)
Herpes Simplex/virology , Herpes Zoster/virology , Herpesvirus 3, Human/physiology , Simplexvirus/physiology , Virus Activation , Buttocks/pathology , Buttocks/virology , Child , Face , Female , Herpes Simplex/complications , Herpes Simplex/diagnosis , Herpes Simplex/pathology , Herpes Zoster/complications , Herpes Zoster/diagnosis , Herpes Zoster/pathology , Humans , Immunocompetence , Thigh/pathology , Thigh/virologyABSTRACT
We report a case of a 24-year-old female patient with a history of giant and multiple lesions of condylomata acuminata on the vulva and breasts which were treated by surgical excision.
