ABSTRACT
Six previously undescribed compounds, named monaxanthones A and B, monaphenol A, monathioamide A, monaprenylindole A, and monavalerolactone A, were isolated from the culture of a marine-sourced bacterium Pseudomonas sp. ZZ820R in rice medium. Their structures were elucidated based on the HRESIMS data, NMR and MS-MS spectroscopic analyses, optical rotation and ECD calculations. Monathioamide A is an unprecedented sulfur-contained compound and monavalerolactone A represents the first example of this type of natural products. Monaprenylindole A showed antibacterial activity against methicillin-resistant Staphylococcus aureus.
Subject(s)
Anti-Bacterial Agents/pharmacology , Lactones/pharmacology , Methicillin-Resistant Staphylococcus aureus/drug effects , Pseudomonas/chemistry , Thioamides/pharmacology , Anti-Bacterial Agents/isolation & purification , Aquatic Organisms/chemistry , Cell Line, Tumor , China , Escherichia coli/drug effects , Humans , Lactones/isolation & purification , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Molecular Structure , Soil Microbiology , Tandem Mass Spectrometry , Thioamides/isolation & purificationSubject(s)
Antineoplastic Agents/isolation & purification , Antineoplastic Agents/metabolism , Biosynthetic Pathways , Multigene Family , Peptides, Cyclic/biosynthesis , Peptides, Cyclic/isolation & purification , Streptomyces lividans/metabolism , Thioamides/isolation & purification , Antineoplastic Agents/chemistry , Humans , Magnetic Resonance Spectroscopy , Molecular Structure , Peptides, Cyclic/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Streptomyces lividans/genetics , Thioamides/chemistryABSTRACT
A sorbent extraction procedure for lead, iron, cadmium and manganese ions on Sepabeads SP70 adsorption resin has been presented prior to their flame atomic absorption spectrometric determinations. By the passage of aqueous samples including analyte ions-rubeanic acid chelates through Sepabeads SP70 column, metal chelates adsorb quantitatively and almost all matrix elements will pass through the column to drain. The influence of potential interfering ions was also studied. The validation of the method was made though the analysis of LGC 6010 Hard drinking water, SRM 1577b Bovine liver and GBW 07603 Bush branches and leaves standard reference materials (SRM). The method was applied to the determination of analyte ions from various water, wastewater, cow meat and milk, red wine, and tobacco samples with successfully results.
