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1.
Sci Rep ; 11(1): 8150, 2021 04 14.
Article in English | MEDLINE | ID: mdl-33854148

ABSTRACT

Cortical spreading depolarization (CSD) is a propagating wave of tissue depolarization characterized by a large increase of extracellular potassium concentration and prolonged subsequent electrical silencing of neurons. Waves of CSD arise spontaneously in various acute neurological settings, including migraine aura and ischemic stroke. Recently, we have reported that pan-inhibition of adrenergic receptors (AdRs) facilitates the normalization of extracellular potassium after acute photothrombotic stroke in mice. Here, we have extended that mechanistic study to ask whether AdR antagonists also modify the dynamics of KCl-induced CSD and post-CSD recovery in vivo. Spontaneous neural activity and KCl-induced CSD were visualized by cortex-wide transcranial Ca2+ imaging in G-CaMP7 transgenic mice. AdR antagonism decreased the recurrence of CSD waves and accelerated the post-CSD recovery of neural activity. Two-photon imaging revealed that astrocytes exhibited aberrant Ca2+ signaling after passage of the CSD wave. This astrocytic Ca2+ activity was diminished by the AdR antagonists. Furthermore, AdR pan-antagonism facilitated the normalization of the extracellular potassium level after CSD, which paralleled the recovery of neural activity. These observations add support to the proposal that neuroprotective effects of AdR pan-antagonism arise from accelerated normalization of extracellular K+ levels in the setting of acute brain injury.


Subject(s)
Adrenergic Antagonists/administration & dosage , Cortical Spreading Depression/drug effects , Potassium Chloride/adverse effects , Thrombotic Stroke/drug therapy , Adrenergic Antagonists/pharmacology , Animals , Calcium/metabolism , Disease Models, Animal , Female , Male , Mice , Mice, Transgenic , Potassium Chloride/pharmacology , Recovery of Function , Thrombotic Stroke/etiology , Thrombotic Stroke/metabolism , Thrombotic Stroke/physiopathology
2.
Front Immunol ; 11: 1746, 2020.
Article in English | MEDLINE | ID: mdl-33013828

ABSTRACT

Innate immune memory is a part of the innate immune system that facilitates the elimination of pathogens. However, it may exacerbate neuropathology. In this study, we found that innate immune memory is detrimental in stroke, because it promotes the acute immune response and exacerbates ischemic infarcts. Mesenchymal stem cell therapy has been widely studied for its therapeutic potential in various diseases including stroke, but whether it diminishes innate immune memory has not been studied. Here, our study demonstrates that, after the activation of innate immune memory by lipopolysaccharide, mesenchymal stem cell therapy can diminish innate immune memory though down-regulation of H3 methylation and subsequently protect against stroke. Our results demonstrate that innate immune memory is detrimental in stroke, and we describe a novel potential therapeutic target involving the use of mesenchymal stem cells to treat stroke patients.


Subject(s)
Brain/drug effects , Immunity, Innate/drug effects , Immunologic Memory/drug effects , Ischemic Stroke/surgery , Lipopolysaccharides/toxicity , Mesenchymal Stem Cell Transplantation , Thrombotic Stroke/surgery , Umbilical Cord/cytology , Animals , Brain/immunology , Brain/metabolism , Brain/pathology , Cells, Cultured , Cytokines/metabolism , Disease Models, Animal , Humans , Inflammation Mediators/metabolism , Ischemic Stroke/immunology , Ischemic Stroke/metabolism , Ischemic Stroke/pathology , Lipopolysaccharides/immunology , Male , Mice, Inbred C57BL , Microglia/drug effects , Microglia/immunology , Microglia/metabolism , Microglia/pathology , Thrombotic Stroke/immunology , Thrombotic Stroke/metabolism , Thrombotic Stroke/pathology
3.
Circ Res ; 127(8): 1023-1035, 2020 09 25.
Article in English | MEDLINE | ID: mdl-32762491

ABSTRACT

RATIONALE: Ischemic stroke is a leading cause of morbidity and mortality worldwide. Recanalization of the occluded vessel is essential but not sufficient to guarantee brain salvage. Experimental and clinical data suggest that infarcts often develop further due to a thromboinflammatory process critically involving platelets and T cells, but the underlying mechanisms are unknown. OBJECTIVE: We aimed to determine the role of CD (cluster of differentiation)-84 in acute ischemic stroke after recanalization and to dissect the underlying molecular thromboinflammatory mechanisms. METHODS AND RESULTS: Here, we show that mice lacking CD84-a homophilic immunoreceptor of the SLAM (signaling lymphocyte activation molecule) family-on either platelets or T cells displayed reduced cerebral CD4+ T-cell infiltration and thrombotic activity following experimental stroke resulting in reduced neurological damage. In vitro, platelet-derived soluble CD84 enhanced motility of wild-type but not of Cd84-/- CD4+ T cells suggesting homophilic CD84 interactions to drive this process. Clinically, human arterial blood directly sampled from the ischemic cerebral circulation indicated local shedding of platelet CD84. Moreover, high platelet CD84 expression levels were associated with poor outcome in patients with stroke. CONCLUSIONS: These results establish CD84 as a critical pathogenic effector and thus a potential pharmacological target in ischemic stroke.


Subject(s)
Blood Platelets/metabolism , CD4-Positive T-Lymphocytes/metabolism , Infarction, Middle Cerebral Artery/metabolism , Inflammation/metabolism , Signaling Lymphocytic Activation Molecule Family/metabolism , Thrombotic Stroke/metabolism , Aged , Aged, 80 and over , Animals , Blood Coagulation , CD4-Positive T-Lymphocytes/immunology , Chemotaxis, Leukocyte , Cytokines/metabolism , Disease Models, Animal , Female , Homeodomain Proteins/genetics , Homeodomain Proteins/metabolism , Humans , Infarction, Middle Cerebral Artery/genetics , Infarction, Middle Cerebral Artery/immunology , Inflammation/genetics , Inflammation/immunology , Male , Mice, Inbred C57BL , Mice, Knockout , Middle Aged , Prospective Studies , Signal Transduction , Signaling Lymphocytic Activation Molecule Family/genetics , Thrombotic Stroke/genetics , Thrombotic Stroke/immunology
4.
Neuroreport ; 31(13): 971-978, 2020 09 09.
Article in English | MEDLINE | ID: mdl-32694311

ABSTRACT

We recently reported that upregulation of the constitutively active ras homolog enriched in brain [Rheb(S16H)], which induces the activation of the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway, can protect adult neurons, mediated by the induction of neurotrophic factors, such as brain-derived neurotrophic factor (BDNF), in animal models of neurodegenerative diseases. Here we show that neuronal transduction of Rheb(S16H) using adeno-associated virus serotype 1 provides neuroprotection in a mouse model of photothrombosis-induced ischemic stroke. Rheb(S16H)-expressing neurons exhibited neurotrophic effects, such as mTORC1 activation, increases in neuronal size, and BDNF production, in mouse cerebral cortex. Moreover, the upregulation of neuronal Rheb(S16H) significantly attenuated ischemic damage and behavioral impairments as compared to untreated mice, suggesting that Rheb(S16H) upregulation in cortical neurons may be a useful strategy to treat ischemic stroke.


Subject(s)
Motor Cortex/metabolism , Neurons/metabolism , Neuroprotection/genetics , Ras Homolog Enriched in Brain Protein/genetics , Thrombotic Stroke/genetics , Animals , Brain-Derived Neurotrophic Factor/metabolism , Cell Enlargement , Dependovirus , Disease Models, Animal , Fluorescent Dyes , Gait Analysis , Gene Transfer Techniques , Genetic Vectors , Ischemic Stroke/genetics , Ischemic Stroke/metabolism , Ischemic Stroke/pathology , Ischemic Stroke/physiopathology , Mice , Motor Cortex/pathology , Mutation , Neurons/pathology , Rose Bengal , Thrombotic Stroke/metabolism , Thrombotic Stroke/pathology , Thrombotic Stroke/physiopathology , Transduction, Genetic
5.
Mar Drugs ; 18(7)2020 Jul 21.
Article in English | MEDLINE | ID: mdl-32708077

ABSTRACT

Emerging literature suggests that after a stroke, the peri-infarct region exhibits dynamic changes in excitability. In rodent stroke models, treatments that enhance excitability in the peri-infarct cerebral cortex promote motor recovery. This increase in cortical excitability and plasticity is opposed by increases in tonic GABAergic inhibition in the peri-infarct zone beginning three days after a stroke in a mouse model. Maintenance of a favorable excitatory-inhibitory balance promoting cerebrocortical excitability could potentially improve recovery. Brevetoxin-2 (PbTx-2) is a voltage-gated sodium channel (VGSC) gating modifier that increases intracellular sodium ([Na+]i), upregulates N-methyl-D-aspartate receptor (NMDAR) channel activity and engages downstream calcium (Ca2+) signaling pathways. In immature cerebrocortical neurons, PbTx-2 promoted neuronal structural plasticity by increasing neurite outgrowth, dendritogenesis and synaptogenesis. We hypothesized that PbTx-2 may promote excitability and structural remodeling in the peri-infarct region, leading to improved functional outcomes following a stroke. We tested this hypothesis using epicortical application of PbTx-2 after a photothrombotic stroke in mice. We show that PbTx-2 enhanced the dendritic arborization and synapse density of cortical layer V pyramidal neurons in the peri-infarct cortex. PbTx-2 also produced a robust improvement of motor recovery. These results suggest a novel pharmacologic approach to mimic activity-dependent recovery from stroke.


Subject(s)
Cerebral Cortex/drug effects , Excitatory Amino Acid Agonists/administration & dosage , Marine Toxins/administration & dosage , Motor Activity/drug effects , Neuronal Plasticity/drug effects , Oxocins/administration & dosage , Thrombotic Stroke/drug therapy , Animals , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Cerebral Cortex/metabolism , Cerebral Cortex/pathology , Cerebral Cortex/physiopathology , Disease Models, Animal , Injections , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Mice, Transgenic , Recovery of Function , Thrombotic Stroke/metabolism , Thrombotic Stroke/pathology , Thrombotic Stroke/physiopathology
6.
Mol Neurobiol ; 57(7): 3219-3227, 2020 Jul.
Article in English | MEDLINE | ID: mdl-32506381

ABSTRACT

Stroke is one of the leading reasons of human death. Ischemic penumbra that surrounds the stroke-induced infarction core is potentially salvageable, but molecular mechanisms of its formation are poorly known. Histone acetylation induces chromatin decondensation and stimulates gene expression. We studied the changes in the levels and localization of histone acetyltransferases HAT1 and PCAF in penumbra after photothrombotic stroke (PTS, a stroke model). In PTS, laser irradiation induces local occlusion of cerebral vessels after photosensitization by Rose Bengal. HAT1 and PCAF are poorly expressed in normal cortical neurons and astrocytes, but they are overexpressed 4-24 h after PTS. Their predominant localization in neuronal nuclei did not change after PTS, but their levels in the astrocyte nuclei significantly increased. Western blotting showed the increase of HAT1 and PCAF levels in the cytoplasmic fraction of the PTS-induced penumbra. In the nuclear fraction, PCAF level did not change, and HAT1 was overexpressed only at 24 h post-PTS. PTS-induced upregulation of HAT1 and PCAF in the penumbra was mainly associated with overexpression in the cytoplasm of neurons and especially astrocytes. HAT1 and PCAF did not co-localize with TUNEL-positive cells that indicated their nonparticipation in PTS-induced apoptosis.


Subject(s)
Astrocytes/metabolism , Cerebral Cortex/metabolism , Histone Acetyltransferases/metabolism , Neurons/metabolism , Thrombotic Stroke/metabolism , p300-CBP Transcription Factors/metabolism , Animals , Astrocytes/pathology , Cell Nucleus/metabolism , Cerebral Cortex/pathology , Cytoplasm/metabolism , Male , Neurons/pathology , Rats , Rats, Wistar , Thrombotic Stroke/pathology
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