Exploration of effect of Odanacatib on inhibiting orthodontic recurrence in rats and on CatK and IGF-1 mRNA.

OBJECTIVE: This study aimed to investigate the inhibitory effect of Odanacatib on orthodontic recurrence in rats. MATERIALS AND METHODS: Forty rats were selected to establish a planting anchorage molar movement model, and 50 g of force was used for the mesial movement of the right maxillary first molar. Forty rats were randomly divided into the observation group (n=20) and control group (n=20). Odanacatib (60 µl, 1.25 µM) was locally injected into the mucoperiosteum around the right maxillary first molar of rats in the experimental group, and an equal amount of normal saline was injected into rats in the control group. A Vernier caliper was used for measuring the recurrence movement distance and recurrence rate of rats, Micro-CT for scanning the bone mineral density (BMD) and bone volume fraction (BVF) of the alveolar bone, TRAP special staining for observing changes in osteoclasts and quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) for detecting the mRNA expressions of cathepsin K (CatK) and insulin-like growth factor 1 (IGF-1) in periodontal tissues. RESULTS: After 3 weeks of modeling, the movement distance of the first molar of rats in the two groups was 1.16±0.19 mm. The molar movement distance and recurrence rate of rats were significantly higher in the control group than those in the observation group (p<0.05). The BMD and BVF of the alveolar bone of rats were markedly lower in the control group than those in the observation group (p<0.05). There was no statistically significant difference in the number of osteoclasts between the observation group (26.15±3.92) and the control group (27.01±2.74) (t=0.882, p=0.383). The CatK mRNA expression of rats was remarkably lower in the observation group than that in the control group (p<0.05). The IGF-1 mRNA expression of rats was significantly higher in the observation group than that in the control group (p<0.05). CONCLUSIONS: By promoting the IGF-1 mRNA expression and increasing the BMD and BVF of the alveolar bone, Odanacatib inhibits orthodontic recurrence and has no effect on osteoclast activity.

Desplazamiento accidental del tercer molar al seno maxilar: protocolo de actuación / Accidental displacement of the third molar into the maxillary sinus: action protoco

Introducción: El desplazamiento accidental del tercer molar hacia el seno maxilar es una complicación de la cirugía bucal. La extracción de estos dientes generalmente es compleja debido a la escasa visibilidad, el espacio limitado y la ubicación espacial del diente, por lo que se requiere tener un cuidado meticuloso durante el tratamiento. Objetivo: El objetivo de este artículo ha sido el de establecer un protocolo de actuación basado tanto en el tratamiento quirúrgico como farmacológico, y el seguimiento que este tipo de complicaciones necesita según la literatura. Caso clínico: Se presenta el caso clínico de un varón de 17 años de edad, sin antecedentes médicos de interés, que fue remitido por su odontólogo para proceder a la valoración y extracción quirúrgica del tercer molar superior izquierdo que había sido desplazado al interior del seno maxilar tras el intento de exodoncia, y que producía sintomatología compatible con sinusitis aguda. La exploración radiológica descartó la afectación del resto de senos paranasales y confirmó la obstrucción casi completa del seno maxilar izquierdo. La extracción del cordal se realizó mediante ostectomía de la pared anterior del seno, pautándose tratamiento farmacológico postoperatorio y controles periódicos durante el primer año. Discusión: La terapéutica recomendada para esta complicación incluye la recuperación inmediata del diente, o la extracción posterior mientras la fibrosis se desarrolla en torno al diente desplazado, según sea necesario, tomando en cuenta la infección, el rango limitado del movimiento de la mandíbula, o malestar psicológico del paciente. El tratamiento médico y farmacológico que debe prescribirse según la literatura revisada, para facilitar el drenaje sinusal y el control de la infección. Conclusión: Para evitar este tipo de complicaciones, conviene realizar un estudio radiológico completo en los casos de cercanía del tercer molar superior en las inmediaciones del seno maxilar, y valorar la disposición e inclinación del mismo

Introduction: Accidental displacement of the third molar into the maxillary sinus is one of the complications of oral surgery. These extractions are usually complex due to some factors such as low visibility, limited space and the location of the tooth; so special care on these treatments is required. Objective: To establish an action protocol based on a surgical as well as a pharmacological treatment, and the monitoring that this kind of complications need according to the literature. Clinical case: A 17-year old man, with a noncontributory medical history, was referred to our clinic by a colleague to evaluate and perform a surgical extraction of his upper left third molar, which had been displaced into the maxillary sinus after a failed extraction, and was generating symptoms of acute sinusitis. The radiographic examination dismissed the affectation of the rest of the paranasal sinuses and confirmed the almost totally blockage of the left maxillary sinus. The extraction of this third molar took place by means of an anterior-wall ostectomy of the sinus; afterwards a pharmacological treatment was prescribed and regular control visits along the first year were planned. Discusion:The suggested treatment for this kind of complications involves the immediate recuperation of the tooth or its later extraction meanwhile the fibrosis forms around the displaced tooth, as needed. We have to take into account the infection, the limited mandibular range of movement as well as the patient's psychological discomfort. The medical and pharmacological treatment should be prescribed following the reviewed literature, in order to obtain the sinusal drainage and the infection control. Conclusion: It is convenient to carry out an exhaustive radiological examination in case of close relation between the maxillary third molar and the maxillary sinus as well as evaluate its position and inclination to avoid this kind of complications

Buccodental side effects of sunitinib in patients with metastatic renal cell carcinoma.

BACKGROUND: Sunitinib is a tyrosine kinase inhibitor approved for the treatment of renal cell carcinoma (RCC). Few data evaluated severe buccodental adverse events. The aim of this study was to evaluate sunitinib buccodental toxicity in patients with metastatic RCC and to compare it with that of standard chemotherapy in patients with other solid cancers. METHODS: Patients with RCC treated with sunitinib and patients with other solid tumours treated with chemotherapy were followed for 3 months. Data on dental appliances, oral hygiene/care practices before and during treatment were collected. RESULTS: A total of 116 patients were included (58 RCC treated by sunitinib: group S, and 58 treated by chemotherapy: group C). No differences in dental care habits were noted before treatment. In group S, patients reported significantly more frequent pain (P<0.01), teeth instability (P=0.01), gingival bleeding (P=0.01) and change in teeth colour (P=0.02). In all, 58% of patients in this group had to modify their diet (P<0.01). Frequency of dentist visits for teeth removal was increased (25% vs 8%, P=0.01). CONCLUSION: Sunitinib seems to increase buccodental toxicity as compared with chemotherapy. This finding emphasises the need for optimal dental care and standardised dental follow-up in patients treated with sunitinib.

Effects of relaxin on relapse and periodontal tissue remodeling after experimental tooth movement in rats.

The relapse of teeth that have moved during orthodontic treatment is a major clinical issue with respect to the goals of successful treatment. Relaxin has an influence on many physiologic processes, such as collagen turnover. In this study, we determined the effects of relaxin on the relapse and remodeling of periodontal tissue after experimental tooth movement in rats, and we explored the molecular mechanism underlying these processes. To induce experimental tooth movement in rats, 10 g of orthodontic force was applied to the molars. After 14 days, the spring was removed, and then animals began receiving relaxin at a dose of 500 ng/ml for 1 week. The results were evaluated by micro-computed tomography and immunofluorescence staining. In addition, the effects of matrix metalloproteinase (MMP)-1 and MMP-8 production were investigated in human periodontal ligament (hPDL) cells in vitro. The expression of MMP-1 and MMP-8 was analyzed by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Furthermore, we demonstrated the signaling pathways involved in relaxin-regulated MMPs expression. The relapse distances and percentages were significantly decreased in the experimental group compared with the controls in vivo. A double-immunofluorescence analysis for Col-I/MMP-1 and Col-I/MMP-8 detected the expression of relaxin in the PDL. Relaxin significantly increased the MMP-1 and MMP-8 expression in a time-dependent manner in hPDL cells in vitro. Furthermore, a p38 inhibitor (SB203580) significantly inhibited the MMP-1 and MMP-8 expression. Our results indicated that relaxin modulates the collagen metabolism, and this hormone may therefore be useful to prevent orthodontic relapse following orthodontic treatment.