ABSTRACT
Chemical diversity of secondary metabolites provides a considerable variety of pharmacological actions with a significant extension due to their combinations in plant extracts. Production of plant-derived medicinal products in cell cultures has advantages because of the efficient use of different biotic and abiotic elicitors and better control of the developmental processes. Using PASS software, we predicted biological activity spectra for phytoconstituents identified in cell cultures of Panax japonicus (12 molecules), Tribulus terrestris (4 molecules), and Dioscorea deltoidea (3 molecules). Mechanisms of action associated with the antihypoxic effect were predicted for the majority of molecules. PharmaExpert software allowed analyzing possible synergistic or additive effects of the combinations of phytoconstituents associated with the antihypoxic action. Experimental studies of the antihypoxic effect of the plants' extracts in water and ethanol have been performed in 3 animal models: Acute asphyctic hypoxia (AAH), Acute haemic hypoxia (AHeH), and Acute histotoxic hypoxia (AHtH). Effects of Panax japonicus and Tribulus terrestris preparations exceeded the activity of the reference drug Mexidol in the AHtH model. In the AHeH model, all preparations demonstrated moderate activity; the most potent has been observed for Dioscorea deltoidea. Thus, we found that experimental studies in animal models have confirmed the in silico prediction.
Subject(s)
Cell Culture Techniques , Computer Simulation , Dioscorea/cytology , Panax/cytology , Phytochemicals/pharmacology , Tribulus/cytology , Animals , Biomass , Cell Hypoxia/drug effects , Cell Survival/drug effects , Male , Mice , Phytochemicals/chemistry , SoftwareABSTRACT
OBJECTIVE: To study the pharmacognosy of Nitraria tangutorum Bobr. systematically. METHODS: Macroscopic, microscopic identifications, physicochemical identification and UV spectrometry methods were carried out. RESULTS: The macroscopical, microscopical and physicochemical characteristics of Nitraria tangutorum Bobr. were decribed in details. CONCLUSION: The results can be taken as the reference for identifying the species, analyzing the quality and laying down quality standard of the crude drug.
Subject(s)
Plants, Medicinal/anatomy & histology , Tribulus/anatomy & histology , China , Fruit/anatomy & histology , Fruit/chemistry , Fruit/cytology , Pharmacognosy , Plants, Medicinal/chemistry , Plants, Medicinal/cytology , Powders , Spectrophotometry, Ultraviolet , Tribulus/chemistry , Tribulus/cytologyABSTRACT
The descriptions, microscopic and TLC identification of Tribulus terrestris L. were carried out. It provided evidences for the identification of Tribulus terrestris L.
