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Pol Arch Weter ; 27(4): 15-23, 1987.
Article in Polish | MEDLINE | ID: mdl-3508516

ABSTRACT

The object of this study was to determine of aminoacids and sugar contents of T. verrucosum mycelium and of homologous purified trichophytin Tv-GP. The analysis of aminoacids contents was performed by ion-exchange chromatography on Czechoslovak analyser (type AAA-881) in acid and alkaline hydrolizates. Sugar were identified on gas chromatograph (PYE-105) by Sawerdeker and Sloneker technique. Seventeen identical aminoacids were determined in mycelium and trichophytin, but some quantity differences were observed. In mycelium there was 40.8% aminoacid nitrogen and 59.2% aminosaccharide nitrogen. In trichophytin, however, 93.5% nitrogen originated from aminoacids and only 6.5% from aminosaccharides. In both analized materials hexoses dominated: mannose, glucose and galactose. Besides trace quantities of D-glycero-D-mannoheptose, L-glycero-D-mannoheptose and L-glycero-D-glucoheptose were identified.


Subject(s)
Amino Acids/metabolism , Antigens, Fungal/analysis , Carbohydrate Metabolism , Trichophytin/analysis , Trichophyton/metabolism , Amino Acids/analysis , Carbohydrates/analysis , Chromatography, Gas , Chromatography, Ion Exchange , In Vitro Techniques , Trichophyton/immunology
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