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1.
Electrophoresis ; 37(2): 339-46, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26462723

ABSTRACT

Electromembrane extraction (EME) of model analytes was carried out using a virtually rotating supported liquid membrane (SLM). The virtual (nonmechanical) rotating of the SLM was achieved using a novel electrode assembly including a central electrode immersed inside the lumen of the SLM and five counter electrodes surrounding the SLM. A particular electronic circuit was designed to distribute the potential among five counter electrodes in a rotating pattern. The effect of the experimental parameters on the recovery of the extraction was investigated for verapamil (VPL), trimipramine (TRP), and clomipramine (CLP) as the model analytes and 2-ethyl hexanol as the SLM solvent. The results showed that the recovery of the extraction is a function of the angular velocity of the virtual rotation. The best results were obtained at an angular velocity of 1.83 RadS(-1) (or a rotation frequency of 0.29 Hz).The optimization of the parameters gave higher recoveries up to 50% greater than those of a conventional EME method. The rotating also allowed the extraction to be carried out at shorter time (15 min) and lower voltage (200 V) with respect to the conventional extraction. The model analytes were successfully extracted from wastewater and human urine samples with recoveries ranging from 38 to 85%. The RSD of the determinations was in the range of 12.6 to 14.8%.


Subject(s)
Chemical Fractionation/instrumentation , Clomipramine/isolation & purification , Electrochemical Techniques/instrumentation , Membranes, Artificial , Trimipramine/isolation & purification , Verapamil/isolation & purification , Anti-Arrhythmia Agents/isolation & purification , Anti-Arrhythmia Agents/urine , Antidepressive Agents, Tricyclic/isolation & purification , Antidepressive Agents, Tricyclic/urine , Clomipramine/urine , Electrodes , Equipment Design , Humans , Limit of Detection , Rotation , Trimipramine/urine , Urinalysis/instrumentation , Verapamil/urine , Wastewater/analysis , Water Pollutants, Chemical/isolation & purification , Water Pollutants, Chemical/urine , Water Purification/instrumentation
2.
Anal Bioanal Chem ; 399(10): 3555-64, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21298415

ABSTRACT

A novel method based on three-phase hollow fiber microextraction technique (HF-LPME) coupled with electrospray ionization-ion mobility spectrometry (ESI-IMS) was developed for the simultaneous determination of two antidepressant drugs (trimipramine and desipramine) in urine and plasma samples. The effects of various parameters such as type of organic solvent, composition of donor and acceptor phase, stirring rate, salt addition, extraction time, and temperature were investigated. Under the optimized conditions, the relative standard deviation was in the range of 5-6%, and the method quantitation limit (MQL) of utilizing HF-LPME/ESI-IMS was 5 µg/L for both drugs. The relative recoveries obtained by the proposed method from urine and plasma samples were in the range 94% to 97% for trimipramine and 92% to 96% for desipramine. Finally, the feasibility of the proposed method was successfully confirmed by extraction and determination of trace amounts of trimipramine and desipramine in biological samples without any significant matrix effect.


Subject(s)
Antidepressive Agents/blood , Antidepressive Agents/urine , Desipramine/blood , Desipramine/urine , Solid Phase Microextraction/methods , Spectrometry, Mass, Electrospray Ionization/methods , Trimipramine/blood , Trimipramine/urine , Antidepressive Agents/isolation & purification , Desipramine/isolation & purification , Humans , Solid Phase Microextraction/instrumentation , Trimipramine/isolation & purification
3.
Res Commun Chem Pathol Pharmacol ; 82(1): 111-20, 1993 Oct.
Article in English | MEDLINE | ID: mdl-8272570

ABSTRACT

In vitro metabolism of the tricyclic antidepressant trimipramine using a commercial preparation of human CYP2D6 isozyme expressed in a human cell line is described. 2-Hydroxytrimipramine and a previously unreported metabolite, 2,10- or 2,11-dihydroxytrimipramine were isolated. Their structures were determined by gas chromatography/mass spectroscopy of underivatized and derivatized extracts. Acetylation of the new metabolite resulted in dehydration at C10 to give 10,11-dehydro-2-acetoxytrimipramine. No N-dealkylation of trimipramine was observed. Prior administration of quinidine produced a large reduction in the metabolic oxidation of trimipramine with CYP2D6 while prior administration of quinine had no effect. The use of this CYP2D6 isozyme preparation in vitro is of value in the identification of possible in vivo substrates for the human CYP2D6 isozyme.


Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Isoenzymes/metabolism , Mixed Function Oxygenases/metabolism , Trimipramine/metabolism , Cell Line , Cytochrome P-450 CYP2D6 , Gas Chromatography-Mass Spectrometry , Humans , Hydroxylation , Substrate Specificity , Trimipramine/analogs & derivatives , Trimipramine/isolation & purification
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