ABSTRACT
This study has presented specific features that are examined to remove the Trypan blue dye from the waste using Luffa sponge (LS) and modified Luffa sponge with zinc nanoparticles (ZnNPs). Peroxidase enzyme was obtained from Euphorbia amygdaloides plant and it was used with the green synthesis of Zn nanoparticles. Luffa sponge was used to be a support material for immobilized nanoparticles and it also used in remediation work. The obtained membrane forms, fibrous materials, (LS, ZnNPs-LS) were characterized with SEM and XRD. LS and ZnNPs-LS were employed as adsorbent to be used for the removal of Trypan blue dye from aqueous via batch studies. Measurements were made for the equilibrium, pH, temperature, concentration of dye with UV-visible spectrometer (590nm; for Trypan blue dye). The optimum removal of Trypan blue dye was found at pH7, the equilibrium was attained within 30min. The thermodynamic properties ΔG0, ΔH0, and ΔS0 showed that adsorption of Trypan blue dye onto LS and ZnNPs-LS were spontaneous and endothermic. The equilibrium isotherm data were analyzed using Langmuir and Freundlich models and the sorption process was described by the Langmuir isotherm with maximum monolayer adsorption capacity of 45.32 and 47.3mg/g for LS and LS-ZnNPs at 303±1°K, respectively.
Subject(s)
Coloring Agents/isolation & purification , Luffa/chemistry , Nanoparticles/chemistry , Trypan Blue/isolation & purification , Zinc/chemistry , Adsorption , Coloring Agents/chemistry , Hydrogen-Ion Concentration , Models, Theoretical , Nanoparticles/ultrastructure , Temperature , Time Factors , Trypan Blue/chemistry , X-Ray DiffractionABSTRACT
The photo-assisted deposition (PAD) and impregnation (Img) synthesis of nano-sized Cu metal on TiO2-SiO2 are reported. The prepared catalysts were characterized by different techniques such as XRD, EXAFS, TEM and nitrogen adsorption analysis. Photocatalytic reactivity using Cu-TiO2-SiO2 catalysts under visible-light condition on the oxidation of direct blue dye with O2 reaction was evaluated. The results have shown notable photocatalytic activity of PAD-Cu/TiO2-SiO2 which was 1.6 and 10 times higher than that of Img-Cu/TiO2-SiO2 and TiO2-SiO2, respectively.
Subject(s)
Copper/chemistry , Metal Nanoparticles/chemistry , Titanium/chemistry , Trypan Blue/chemistry , Catalysis , Copper/radiation effects , Light , Materials Testing , Metal Nanoparticles/radiation effects , Photochemistry/methods , Trypan Blue/isolation & purification , Trypan Blue/radiation effectsABSTRACT
A new method for extraction and concentration of organic dyes that uses a reagent composed of a nonionic detergent mixed with an alcohol is described. We have observed that water-soluble organic dyes are also soluble in nonionic detergents and can be extracted by adding salt, which separates the dye-detergent component from the aqueous phase. We have also found that mixing nonionic detergents with alcohols markedly reduces their viscosity and produces stable, free-flowing, and effective reagents for color extraction. On the basis of these observations, we used a mixture of Triton X-100 and 1-butanol and observed that water-soluble natural and synthetic chromophores, as well as dyes generated in biochemical reactions, can be extracted, concentrated, and analyzed spectrophotometrically. Trypan blue and phenol red are used as examples of synthetic dyes, and red wine is used as an example of phenolic plant pigments. Applications for quantification of nitric oxides and sialic acids are described in more detail and show that as little as 0.15 nmol of nitric oxide and 0.20 nmol of sialic acid can be detected. A major advantage of this method is its ability to concentrate chromophores from dye-containing solutions that otherwise cannot be measured because of their low concentrations.
Subject(s)
Colorimetry/methods , Nitric Oxide/analysis , Phenolsulfonphthalein/isolation & purification , Pigments, Biological/isolation & purification , Sialic Acids/analysis , Trypan Blue/isolation & purification , Alcohols/chemistry , Detergents/chemistry , Phenolsulfonphthalein/chemistry , Pigments, Biological/chemistry , Solubility , Trypan Blue/chemistrySubject(s)
Coloring Agents/isolation & purification , Sterilization , Trichoderma , Trypan Blue/isolation & purification , Water Pollutants, Chemical/isolation & purification , Adsorption , Coloring Agents/chemistry , Hydrogen-Ion Concentration , Industrial Waste , Mycelium/chemistry , Textiles , Trypan Blue/chemistry , Waste Disposal, Fluid/methods , Water Purification/methodsABSTRACT
Three coloured substances frequently present as contaminants in commercial samples of trypan blue have been identified as those monoazo dyes in which 4-amino-3,3'-dimethyl-biphenyl, 4-amino-3,3'-dimethyl-4'-hydroxy-biphenyl or omicroc-tolidine are coupled to H-acid. These dyes have been synthesized and, together with purified samples of trypan blue, tested for teratogenic activity in mice and oncogenic activity in rats. Unpurified trypan blue was both teratogenic and oncogenic; purified trypan blue, was teratogenic but only weakly oncogenic; the monoazo dyes possessed neither activity. It is concluded that the main blue component of trypan blue is the teratogenic principle and that some as yet unidentified component of the purple fraction either is the main oncogenic principle or potentiates the action of the blue component.
