EUCOLEUS BOEHMI INFECTION IN THE NASAL CONCHAE AND PARANASAL SINUSES OF RED FOX (VULPES VULPES) ON PRINCE EDWARD ISLAND, CANADA.

Eucoleus boehmi (Nematoda: Capillariidae) occurs in the nasal conchae and paranasal sinuses of wild and domestic canids. We surveyed the red fox ( Vulpes vulpes ) on Prince Edward Island, Canada, for E. boehmi infection and characterized the associated histopathology. Nasal capillarid infections were detected based on histologic examination of three coronal sections of the nasal cavity and by centrifugal flotation examination (CFE) of rectal feces. Capillarids were detected in histologic sections in 28 of 36 (78%) foxes; detection occurred most frequently in the caudal section (28 foxes) and least in the rostral section (10 foxes). Adult worm morphology was typical for capillarids (stichosome esophagus, bacillary bands, bipolar plugged eggs); E. boehmi eggs were specifically identified based on the characteristic pitted shell wall surface. Adult worms were detected in histologic sections in all 28 and E. boehmi eggs in 21 of the positive foxes. No eggs of Eucoleus aerophilus were observed in any of the sections. Affected foxes had an eosinophilic and lymphoplasmacytic rhinitis with goblet cell hyperplasia. Eggs of E. aerophilus were detected on CFE in 20 of 36 (56%) foxes; 19 of the histologically positive foxes were coinfected with E. aerophilus. Eggs of E. boehmi were detected on CFE in 26 of 36 (72%) foxes and were consistent in size and morphology with those described from wild canids, but they differed from those reported from cases of infection in dogs. Prevalence based on identification of eggs on histologic section or CFE indicated 27 of 36 (75%) red foxes examined were infected with E. boehmi.

Nasal leech infestation: report of seven leeches and literature review.

Nasal leech infestation rarely occurs in society today and it is usually reported as an anecdote. In this study, we present seven nasal leeches in six patients from 1984 to 2008. All patients initially presented with epistaxis. Four patients were less than 8 years old and two patients were older than 60 years old. All patients had spent time in rural streams 2 weeks to 2 months before the symptoms occurred. All of the seven leeches were removed smoothly and one leech migrated to the oropharynx during the operation. The length of the leeches ranged from 2 to 12 cm with an average length of 4.6 cm. Attention should be given to nasal leech infestation, especially in children and senior citizens who have visited rural streams and have been exposed to freshwater. One of our patients had two leeches lodged in his nasal cavities. Therefore, it is also important to re-examine both nasal cavities after the removal of one leech.

In vitro evaluation of bovine turbinate cells and Sarcocystis falcatula proliferation in response to serum-free medium and other media supplements.

A serum-free medium (SFM) was evaluated for the growth of bovine turbinate (BT) cells used for the production of Sarcocyvstis falcatula merozoites. Serum free cultures used to propagate S. falcatula were compared to cultures maintained in media supplemented with fetal calf serum (FCS) or horse serum (HS). Serum free cultures were more.effective and very promisin, than the others in supporting the proliferation of S. falcatula merozoites. However, the serum free cultures were unable to adequately support BT cell proliferation compared to the serum-supplemented cultures. No significant differences were seen between cultures supplemented with HS or FCS used for the production of S. falcatula merozoites or BT cells. The rate of BT cell proliferation in response to SFM and different media supplements was assessed in a 96-well plate format using methylene blue staining assay. This technique was superior to manual counting method and allowed quick and accurate quantitative comparison bet-ween the response of proliferating BT cells to different growth conditions

Nasal myiasis by fruit fly larvae: a case report.

We report a case of human nasal myiasis caused by fruit fly larvae in a 33-year-old man who visited the south coast of Turkey. The fruit fly (Drosophila melanogaster) is endemic in the southern part of Turkey. Infestation with fruit fly larvae must be considered in patients who are visiting areas of the world where Drosophila is endemic. This is the first reported case of fruit fly larvae causing human nasal myiasis. The clinical presentation and treatment strategies are discussed.

Determination of the activity of pyrimethamine, trimethoprim, sulfonamides, and combinations of pyrimethamine and sulfonamides against Sarcocystis neurona in cell cultures.

Equine protozoal myeloencephalitis (EPM) is a neurologic syndrome in horses from the Americas and is usually caused by infection with the apicomplexan parasite, Sarcocystis neurona. The activities of pyrimethamine, trimethoprim, sulfachloropyridazine, sulfadiazine, sulfadimethoxine, sulfamethoxazole, sulfamethazine, and sulfathiazole were examined against developing S. neurona merozoites in bovine turbinate cell cultures. A microtiter plate host cell lesion based assay was used to determine the effects of agents on developing merozoites. A cell culture flask assay was used to determine if selective concentrations of the agents killed or only inhibited development of S. neurona. Pyrimethamine was coccidiocidal at 1.0 microg/ml and trimethoprim was coccidiocidal at 5.0 microg/ml. None of the sulfonamides had activity when used alone at 50.0 or 100.0 microg/ml. Combinations of sulfonamides (5.0 or 10.0 microg/ml) with 0.1 microg/ml pyrimethamine demonstrated improved activity.

Localization and migration route of Cephalopina titillator (Diptera: Oestridae) larvae in the head of infested camels (Camelus dromedarius).

The localization and migration routes of Cephalopina titillator larvae were studied in the heads of 45 naturally infested camels. Larvae were located in the pharyngeal cavity (95.6%), the labyrinth of the ethmoid bone (71.1%), the turbinates (28.9%) and the lower nasal meatus (6.7%). Out of 261 first-instar larvae, 73.7% were found in the labyrinth of the ethmoid bone and 26.3% in the turbinates, while 88.9% of second-instar larvae were observed in the pharyngeal cavity and 11.1% in the labyrinth of the ethmoid bone. Most third-instar larvae (91.9%) were found in the pharyngeal cavity, with a few larvae in the labyrinth of the ethmoid bone (6.5%) and lower nasal meatus (1.6%). The first moult occurred in the labyrinth of the ethmoid bone. The second moult was observed in both the labyrinth of ethmoid bone and the pharyngeal cavity. The migration route of these larvae in the infested head was discussed.

[Mast cells and eosinophils of the respiratory mucosa of sheep with Oestrus ovis (Linné, 1761) infection]. / Mastocytes et eosinophiles de la muqueuse respiratoire du mouton infesté par Oestrus ovis (Linné, 1761).

Mast cells and eosinophils have been identified by differential stainings and counted in mucous membrane of nasal septum, turbinates and sinus of 77 ewes naturally infected with Oestrus ovis. Results have been compared with those of nine parasite free lambs. Anova tests indicate significant differences between infected and parasite-free sheep for the cell numbers and their distribution among the septum, the turbinates and the sinus and according to their position in mucous membrane, interglandular chorion of sub-mucosa. In infected sheep, the mean number of mast cells is twice the number present in parasite free animals. The burdens of eosinophils are multiplied by 17 for the septum, 29 for the turbinates and 58 for the sinus. The hypothesis of the development of an hypersensitivity phenomenon in ovine oestrosis is sustained by these results.

The morphology and pathology of Besnoitia sp. in reindeer (Rangifer tarandus tarandus).

Four of five reindeer (Rangifer tarandus tarandus) obtained from a Besnoitia sp.- infected herd at the Assiniboine Park Zoo in Winnipeg, Manitoba, Canada, in October 1989, had evidence of mild dermatitis over the articular surfaces of carpal and tarsal joints. Cysts of Besnoitia sp., either surrounded by inflammatory reactions or without evident host response, were present within the dermis, submucosa of the nasal turbinates, periosteum, tendons, testes and hooves. The light microscopic and histochemical features of Besnoitia sp. from reindeer were indistinguishable from those of other Besnoitia spp. described in cattle, rodents and horses. The Besnoitia sp. cysts and organisms from reindeer were unique in that bradyzoite membrane micropores and cytoplasmic enigmatic bodies were not observed. Two cats were fed cysts of Besnoitia sp. but no oocysts were detected in feces for 90 days post-infection.