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1.
Int J Mol Sci ; 23(23)2022 Nov 27.
Article in English | MEDLINE | ID: mdl-36499160

ABSTRACT

Oxidative stress is associated with aging, cancers, and numerous metabolic and chronic disorders, and phenolic compounds are well known for their health-promoting role due to their free-radical scavenging activity. These phytochemicals could also exhibit pro-oxidant effects. Due to its bioactive phenolic secondary metabolites, Usnea barbata (L.) Weber ex. F.H. Wigg (U. barbata) displays anticancer and antioxidant activities and has been used as a phytomedicine for thousands of years. The present work aims to analyze the properties of U. barbata extract in canola oil (UBO). The UBO cytotoxicity on oral squamous cell carcinoma (OSCC) CLS-354 cell line and blood cell cultures was explored through complex flow cytometry analyses regarding apoptosis, reactive oxygen species (ROS) levels, the enzymatic activity of caspase 3/7, cell cycle, nuclear shrinkage (NS), autophagy (A), and synthesis of deoxyribonucleic acid (DNA). All these studies were concomitantly performed on canola oil (CNO) to evidence the interaction of lichen metabolites with the constituents of this green solvent used for extraction. The obtained data evidenced that UBO inhibited CLS-354 oral cancer cell proliferation through ROS generation (316.67 × 104), determining higher levels of nuclear shrinkage (40.12%), cell cycle arrest in G0/G1 (92.51%; G0 is the differentiation phase, while during G1 phase occurs preparation for cell division), DNA fragmentation (2.97%), and autophagy (62.98%) than in blood cells. At a substantially higher ROS level in blood cells (5250.00 × 104), the processes that lead to cell death-NS (30.05%), cell cycle arrest in G0/G1 (86.30%), DNA fragmentation (0.72%), and autophagy (39.37%)-are considerably lower than in CLS-354 oral cancer cells. Our work reveals the ROS-mediated anticancer potential of UBO through DNA damage and autophagy. Moreover, the present study suggests that UBO pharmacological potential could result from the synergism between lichen secondary metabolites and canola oil phytoconstituents.


Subject(s)
Carcinoma, Squamous Cell , Head and Neck Neoplasms , Mouth Neoplasms , Usnea , Humans , Mouth Neoplasms/drug therapy , Mouth Neoplasms/metabolism , Usnea/chemistry , Usnea/metabolism , Carcinoma, Squamous Cell/drug therapy , Squamous Cell Carcinoma of Head and Neck , Rapeseed Oil/pharmacology , Autophagy , DNA Damage , Reactive Oxygen Species/metabolism , Apoptosis , Plant Extracts/pharmacology , Phenols/pharmacology , DNA/pharmacology , Cell Line, Tumor
2.
Med Sci Monit ; 25: 7527-7537, 2019 Oct 07.
Article in English | MEDLINE | ID: mdl-31589596

ABSTRACT

BACKGROUND Osteosarcoma (OS) is a highly aggressive, metastatic bone tumor with a poor prognosis, and occurs more commonly in children and adolescents. Therefore, new drugs and treatments are urgently needed. In this study, we investigated the effect and potential mechanisms of C18H17NO6 on osteosarcoma cells. MATERIAL AND METHODS Human MNNG osteosarcoma cells were treated with different concentrations of C18H17NO6. The proliferation of the MNNG cells was examined via CCK-8 assay. Cell migration and invasion were tested via wound-healing assay and Transwell migration and invasion assays. ELISA was used to detect MMP-2, MMP-9, and VEGF secretion. Finally, Western blotting and qRT-PCR were used to detect protein and mRNA expressions, respectively. RESULTS C18H17NO6 inhibited MNNG proliferation in a dose- and time-dependent manner and inhibited MMP-2, MMP-9, and VEGF secretion. C18H17NO6 treatment significantly downregulated N-cadherin and Vimentin expression levels and upregulated E-cadherin expression levels in vitro and in vivo. C18H17NO6 inhibited tumor growth in a MNNG xenograft. We also found that C18H17NO6 can significantly reduce the phosphorylation of the PI3K/AKT signaling pathway in vivo and in vitro. However, 740Y-P (a PI3K agonist) had the opposite effect on proliferation, migration and invasion of MNNG cells treated with C18H17NO6. LY294002 (a PI3K inhibitor) downregulated p-PI3K and p-AKT could mimic the inhibitory effect of C18H17NO6. CONCLUSIONS Our results suggest that C18H17NO6 can inhibit human MNNG osteosarcoma cell invasion and migration via the PI3K/AKT signaling pathway both in vivo and in vitro. C18H17NO6 may be a highly effective and low-toxicity natural drug for the prevention or treatment of OS.


Subject(s)
Benzofurans/pharmacology , Osteosarcoma/drug therapy , Usnea/therapeutic use , Animals , Apoptosis/drug effects , Cell Line, Tumor , Cell Movement/drug effects , Cell Proliferation/drug effects , Drugs, Chinese Herbal/pharmacology , Female , Humans , Matrix Metalloproteinase 2/metabolism , Matrix Metalloproteinase 9/metabolism , Medicine, Chinese Traditional/methods , Mice , Neoplasm Invasiveness , Osteosarcoma/metabolism , Osteosarcoma/pathology , Phosphatidylinositol 3-Kinases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , Signal Transduction/drug effects , Usnea/metabolism , Xenograft Model Antitumor Assays
3.
Phytochemistry ; 164: 86-93, 2019 Aug.
Article in English | MEDLINE | ID: mdl-31102999

ABSTRACT

Specialised metabolites in lichens are generally considered repellent compounds by consumers. Nevertheless, if the only food available is lichens rich in specialised metabolites, lichenophages must implement strategies to overcome the toxicity of these metabolites. Thus, the balance between phagostimulant nutrients and deterrent metabolites could play a key role in feeding preferences. To further understand lichen-gastropod interactions, we studied the feeding behaviour and consumption in Notodiscus hookeri, the land snail native to sub-Antarctic islands. The lichen Usnea taylorii was used because of its simple chemistry, its richness in usnic acid (specialised metabolite) and arabitol (primary metabolite) and its presence in snail habitats. Choice tests in arenas with intact lichens versus acetone-rinsed lichens were carried out to study the influence of specialised metabolites on snail behaviour and feeding preference. Simultaneously, usnic acid and arabitol were quantified and located within the lichen thallus using HPLC-DAD-MS and in situ imaging by mass spectrometry to assess whether their spatial distribution explained preferential snail grazing. No-choice feeding experiments, with the pure metabolites embedded in an artificial diet, defined a gradual gustatory response, from strong repellence (usnic acid) to high appetence (D-arabitol). This case study demonstrates that the nutritional activity of N. hookeri is governed by the chemical quality of the food and primarily by nutrient availability (arabitol), despite the presence of deterrent metabolite (usnic acid).


Subject(s)
Benzofurans/metabolism , Snails/metabolism , Sugar Alcohols/metabolism , Usnea/metabolism , Animals , Benzofurans/chemistry , Chromatography, High Pressure Liquid , Mass Spectrometry , Snails/chemistry , Sugar Alcohols/chemistry , Usnea/chemistry
4.
New Phytol ; 224(4): 1657-1667, 2019 12.
Article in English | MEDLINE | ID: mdl-31135955

ABSTRACT

Lichen secondary metabolites show important biological activities as well as pharmaceutical and chemotaxonomic potential. In order to utilize such substances of interest, detailed knowledge of their biosynthetic pathways is essential. 13 CO2 -pulse/chase experiments using intact thalli of the lichen Usnea dasopoga resulted in multiple 13 C-labeled isotopologs in amino acids, but not in the dibenzofuran derivative usnic acid - one of the best-studied lichen metabolites, with considerable and renewed interest for pharmaceutical and lifestyle applications. Spraying an aqueous solution of [U-13 C6 ]glucose onto the thalli of U. dasopoga afforded a specific mixture of multiple 13 C-labeled isotopologs in usnic acid. One- and two-dimensional NMR analysis of the crude lichen extract corroborated the polyketide biosynthetic pathway via methylphloroacetophenone but not via phloroacetophenone. With usnic acid as an exemplar, we provide proof-of-principle experiments that can be used in general to study metabolic pathways and fluxes in intact lichens.


Subject(s)
Benzofurans/metabolism , Lichens/metabolism , Magnetic Resonance Spectroscopy/methods , Carbon Dioxide/metabolism , Carbon Isotopes/analysis , Carbon Isotopes/metabolism , Glucose/metabolism , Metabolic Networks and Pathways , Usnea/metabolism
5.
Z Naturforsch C J Biosci ; 73(7-8): 291-296, 2018 Jul 26.
Article in English | MEDLINE | ID: mdl-29596056

ABSTRACT

This work presents investigations of biologically active metabolites of Usnea barbata lichen. Extraction conditions for usnic acid and other biologically active phytocomponents using various solvent systems were chosen. Modern analytical techniques were used to study composition of the obtained extracts; usnic acid and phenolic compound contents were estimated. Antioxidant activity and antimicrobial properties of lichen dry extract against bacteria Bacillus subtilis and Pseudomonas fluorescens were studied.


Subject(s)
Anti-Bacterial Agents/pharmacology , Antioxidants/pharmacology , Biological Factors/pharmacology , Usnea/metabolism , Anti-Bacterial Agents/isolation & purification , Antioxidants/isolation & purification , Bacillus subtilis/drug effects , Benzofurans/isolation & purification , Benzofurans/pharmacology , Biological Factors/isolation & purification , Microbial Sensitivity Tests , Phenols/isolation & purification , Phenols/pharmacology , Pseudomonas fluorescens/drug effects , Solvents
6.
Molecules ; 23(1)2017 Dec 27.
Article in English | MEDLINE | ID: mdl-29280946

ABSTRACT

Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria, which are considered among the slowest growing organisms, with strong tolerance to adverse environmental conditions. There are about 400 genera and 1600 species of lichens and those belonging to the Usnea genus comprise about 360 of these species. Usnea lichens have been used since ancient times as dyes, cosmetics, preservatives, deodorants and folk medicines. The phytochemistry of the Usnea genus includes more than 60 compounds which belong to the following classes: depsides, depsidones, depsones, lactones, quinones, phenolics, polysaccharides, fatty acids and dibenzofurans. Due to scarce knowledge of metabolomic profiles of Usnea species (U. barbata, U. antarctica, U. rubicunda and U. subfloridana), a study based on UHPLC-ESI-OT-MS-MS was performed for a comprehensive characterization of their secondary metabolites. From the methanolic extracts of these species a total of 73 metabolites were identified for the first time using this hyphenated technique, including 34 compounds in U. barbata, 21 in U. antarctica, 38 in U. rubicunda and 37 in U. subfloridana. Besides, a total of 13 metabolites were not identified and reported so far, and could be new according to our data analysis. This study showed that this hyphenated technique is rapid, effective and accurate for phytochemical identification of lichen metabolites and the data collected could be useful for chemotaxonomic studies.


Subject(s)
Lichens/chemistry , Metabolomics/methods , Plant Extracts/analysis , Tandem Mass Spectrometry/methods , Usnea/chemistry , Usnea/metabolism , Chromatography, High Pressure Liquid/methods , Fungi , Methanol/chemistry , Phytochemicals/metabolism , Plant Extracts/chemistry
7.
Mol Divers ; 21(2): 273-282, 2017 May.
Article in English | MEDLINE | ID: mdl-28130662

ABSTRACT

(+)-Usnic acid ((R)-2,6-diacetyl-7,9-dihydroxy-8,9b-dimethyl-1,3(2H,9bH)-dibenzo-furandione), a dibenzofuran isolated from the lichen Usnea longissima, has been chemically transformed to synthesize a series of sixteen novel triazole analogs by click chemistry approach. The synthesized compounds were tested for their anti-inflammatory potential against the cytokines TNF-[Formula: see text] and IL-1[Formula: see text] in U937 cell lines. The bromo enamines (2a, 2b), azido enamines (3a, 3b) and triazole analogs (4f, 4g, 4h, 5f, 5g and 5h) exhibited promising anti-inflammatory activity against TNF-[Formula: see text] with [Formula: see text] values ranging from 1.40 to 5.70 [Formula: see text]M. Most significantly, the [Formula: see text] values of compounds 5f (1.40 [Formula: see text]M) and 5h (1.88 [Formula: see text]M) are the lowest among the compounds tested and found close to that of standard prednisolone. Hence, these two compounds can be considered as lead molecules for further fine tuning to make highly potent anti-inflammatory therapeutic agents.


Subject(s)
Anti-Inflammatory Agents/chemical synthesis , Anti-Inflammatory Agents/pharmacology , Benzofurans/chemistry , Benzofurans/metabolism , Triazoles/chemical synthesis , Triazoles/pharmacology , Usnea/metabolism , Anti-Inflammatory Agents/chemistry , Click Chemistry , Humans , Interleukin-1beta/metabolism , Triazoles/chemistry , Tumor Necrosis Factor-alpha/metabolism , U937 Cells
8.
Nat Prod Commun ; 11(4): 493-6, 2016 Apr.
Article in English | MEDLINE | ID: mdl-27396202

ABSTRACT

The antimicrobial activity and possible synergistic effects of extracts and compounds isolated from Usnea steineri were evaluated against four resistant bacterial species. A phytochemical study of the acetone extract of U. steineri resulted in the isolation and characterization of difractaic acid and (+)-usnic acid as the main compounds. The acetone extract showed strong activity (less than 10 µg/mL) against resistant strains of Staphylococcus epidermidis and Enterococcus faecalis, and (+)-usnic acid exhibited strong activity against S. epidermidis (MIC 3.12 µg/mL), S. aureus and S. haemnolyticus (MIC 12.5 µg/mL). Combinations of penicillin and tetracycline with (+)-usnic acid did not show any synergistic antimicrobial effects. Difractaic acid was inactive. Our results showed that the acetone extract of U. steineri possesses significant in vitro antimicrobial activity, which is likely related to the presence of (+)-usnic acid.


Subject(s)
Anti-Bacterial Agents/analysis , Benzofurans/isolation & purification , Depsides/isolation & purification , Usnea/chemistry , Depsides/chemistry , Drug Resistance, Multiple, Bacterial , Microbial Sensitivity Tests , Usnea/metabolism
9.
Environ Pollut ; 210: 238-45, 2016 Mar.
Article in English | MEDLINE | ID: mdl-26741560

ABSTRACT

Several pollutants, which include metals, are present in the Antarctic atmosphere, snow, marine and terrestrial organisms. This work reports the elements incorporated by Usnea sp thalli in Potter Peninsula, 25 de Mayo (King George) Island, South Shetlands, Antarctica. Geological origin was analyzed as possible sources of elements. For this purpose, correlations were done using a geochemical tracer, principal component analysis and enrichment factors were computed. Lithophile elements from particulate matter were present in most of the sampling sites. Bromine, Se and Hg showed the highest enrichment factors suggesting other sources than the particulate matter. Mercury values found in Usnea sp were in the same range as those reported for Deception Island (South Shetlands) and remote areas from the Patagonia Andes.


Subject(s)
Lichens/chemistry , Usnea/chemistry , Antarctic Regions , Environmental Monitoring , Mercury/analysis , Metals/analysis , Particulate Matter/analysis , Usnea/metabolism
10.
Mater Sci Eng C Mater Biol Appl ; 58: 204-12, 2016 Jan 01.
Article in English | MEDLINE | ID: mdl-26478304

ABSTRACT

Investigation of an integrated supercritical fluid extraction and supercritical solvent impregnation process for fabrication of microporous polycaprolactone-hydroxyapatite (PCL-HA) scaffolds with antibacterial activity is presented. The HA content and particle size as well as the operating conditions of the integrated process is optimized regarding the amount of impregnated antibacterial agent (Usnea lethariiformis extract) in the PCL-HA matrix, scaffold morphology and antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) strains. High pressure differential scanning calorimetry (HP-DSC) assay reveals that an increasing amount of HA results in decreasing melting temperature as well as crystallinity at an operating pressure of 17 MPa. The PCL-HA composites with micrometric sizes of the HA particles are convenient for being processed by the integrated process due to the simple preparation, a good interaction between the PCL matrix and filler and the advantageous impact on sorption. The scaffold obtained from PCL-HA with 20% of the HA shows the highest impregnation yield at 17 MPa and 35 °C (5.9%) and subsequently also the best bactericidal effect on the tested MRSA strains at an initial bacterial inoculum of 2 × 10(-4)CFU/mL.


Subject(s)
Anti-Bacterial Agents/chemistry , Chromatography, Supercritical Fluid , Durapatite/chemistry , Polyesters/chemistry , Usnea/chemistry , Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Calorimetry, Differential Scanning , Carbon Dioxide/chemistry , Methicillin-Resistant Staphylococcus aureus/drug effects , Microscopy, Electron, Scanning , Pressure , Transition Temperature , Usnea/metabolism
11.
J Environ Radioact ; 138: 177-85, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25244697

ABSTRACT

In 2011-2013, sampling of epiphytic fruticose lichens of the genera Usnea, Bryoria and Alectoria was carried out on Sakhalin and Kuril Islands (the Sakhalin region, Russia) to investigate contamination of these organisms with the Fukushima-derived (134)Cs and (137)Cs. Activities of the radionuclides were determined in all 56 samples of lichens taken for the analysis. After correction for radioactive decay (on 15 March 2011), the activity concentrations ranged from 2.1 Bq kg(-1) (d.w.) to 52 Bq kg(-1) for (134)Cs and from 2.3 Bq kg(-1) to 52 Bq kg(-1) for (137)Cs. Cesium-134 and (137)Cs activities for the whole set of lichens (n = 56) were strongly positively correlated; Spearman's rank correlation coefficient was calculated as 0.991 (P < 0.01). The activity concentrations of (134)Cs and (137)Cs in Usnea lichens from the Sakhalin and Kunashir islands declined with a factor of three in the period from 2011 to 2013. The average biological half-time for both cesium radionuclides in lichens of the genus Usnea is estimated as 1.3 y. The mean of 0.99 ± 0.10 and median of 0.99 were calculated for the decay corrected (134)Cs/(137)Cs activities ratios in the lichens (n = 56). The radionuclides ratio in the lichens did not depend on location of sampling site, species and the time that had passed after the Fukushima accident. The regression analysis has shown the background pre-Fukushima level of (137)Cs of 0.4 ± 0.3 Bq kg(-1), whereas the ratio between the Fukushima-borne (134)Cs and (137)Cs in the lichens was estimated as 1.04. The (134)Cs/(137)Cs activities ratio in lichens from the Sakhalin region is consistent with the ratios reported by others for the heavy contaminated areas on Honshu Island in Japan following the Fukushima accident. The activity concentrations of natural (7)Be in lichens from the Sakhalin region varied between 100 Bq kg(-1) and 600 Bq kg(-1); the activity concentrations did not exhibit temporal variations during a 2y-period of observations. The applicability of epiphytic fruticose lichens as retrospective bio-monitors for the air-borne radiocesium contamination of the environment is discussed.


Subject(s)
Air Pollutants, Radioactive/metabolism , Cesium/metabolism , Fukushima Nuclear Accident , Lichens/metabolism , Radiation Monitoring/methods , Cesium Radioisotopes/metabolism , Retrospective Studies , Russia , Usnea/metabolism
12.
Cell Prolif ; 47(5): 457-64, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25081971

ABSTRACT

OBJECTIVES: Successful cancer treatments still require more compounds to be isolated from natural sources. Thus, we have investigated anti-proliferative/apoptotic effects of methanolic extracts of lichen species Parmelia sulcata Taylor and Usnea filipendula Stirt on human lung cancer (A549, PC3), liver cancer (Hep3B) and rat glioma (C6) cells. MATERIALS AND METHODS: Anti-proliferative effects were monitored by MTT and adenosine triphosphate viability assays, while genotoxic activity was studied using the comet assay. Additionally, cell death mode and apoptosis assays (fluorescence staining, caspase-cleaved cytokeratin 18, caspase-3 activity and PARP cleavage) were performed. RESULTS: Extracts produced anti-population growth effects in a dose-dependent manner (1.56-100 µg/ml) by inducing apoptosis-like cell death. This resulted in the lines having the presence of pyknotic cell nuclei. In addition, significant increase in genetic damage in the cell lines was seen, indicating that DNA damage may have been responsible for apoptotic cell death. CONCLUSION: In this study, methanolic extracts of Parmelia sulcata and Usnea filipendula induced apoptosis-like cell death by causing DNA damage, to cancer cells.


Subject(s)
Apoptosis/drug effects , Ascomycota/metabolism , DNA Damage/drug effects , Neoplasms/drug therapy , Usnea/metabolism , Animals , Brain Neoplasms/drug therapy , Caspase 3/metabolism , Cell Line, Tumor , Cell Proliferation/drug effects , Enzyme Activation/drug effects , Glioma/drug therapy , Humans , Keratin-18/metabolism , Liver Neoplasms/drug therapy , Lung Neoplasms/drug therapy , Poly(ADP-ribose) Polymerases/metabolism , Rats
13.
J Photochem Photobiol B ; 120: 17-28, 2013 Mar 05.
Article in English | MEDLINE | ID: mdl-23416709

ABSTRACT

Three lichen extracts and ten lichenic compounds have been screened for their photoprotective activities. The determination of their Sun Protection Factor (SPF) and Protection Factor-UVA (PF-UVA) values was done in vitro. Among them, a Lasallia pustulata extract and gyrophoric acid exhibited SPF values over 5, which is better than Homosalate (SPF≈4). Their photoprotective properties are only slightly modified after a 2-hours period of irradiation. Salazinic acid and L. pustulata presented characteristics of a UVA booster like the butyl-methoxydibenzoylmethane (Avobenzone) (PF-UVA≈2 vs. 2.8 for Avobenzone). Salazinic acid was a better anion superoxide scavenger than ascorbic acid and none of them exhibited a photosensitizing cytotoxicity by exposing them on HaCaT cells to UVA radiations (photo-irritancy factor PIF<5).


Subject(s)
Sun Protection Factor/methods , Ultraviolet Rays , Usnea/chemistry , Usnea/metabolism , Antioxidants/isolation & purification , Antioxidants/metabolism , Antioxidants/pharmacology , Antioxidants/toxicity , Ascomycota/chemistry , Ascomycota/metabolism , Benzoates/isolation & purification , Benzoates/metabolism , Benzoates/pharmacology , Benzoates/toxicity , Benzofurans/isolation & purification , Benzofurans/metabolism , Benzofurans/pharmacology , Benzofurans/toxicity , Cell Line , Fumarates/isolation & purification , Fumarates/metabolism , Fumarates/pharmacology , Fumarates/toxicity , Humans , Lactones/isolation & purification , Lactones/metabolism , Lactones/pharmacology , Lactones/toxicity , Salicylates/isolation & purification , Salicylates/metabolism , Salicylates/pharmacology , Salicylates/toxicity
14.
Pharm Biol ; 50(8): 968-79, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22775414

ABSTRACT

CONTEXT: Lichens have been used for various purposes such as dyes, perfumes and remedies in folk medicine indicating the pharmaceutical potential of lichens. OBJECTIVE: Lichen growth in nature is very slow. To overcome this major drawback, we standardized the culture media to culture the lichen Usnea complanata (Müll.Arg.) Motyka (Parmeliaceae) for (1) in vitro synthesis of natural lichen substances, and (2) determination of antioxidative and cardiovascular-protective activity of usnic acid and psoromic acid. MATERIALS AND METHODS: Lichen U. complanata has been cultured in fermentor under submerged condition. Antioxidative and cardiovascular-protective activity of the extract and the purified lichen substances usnic and psoromic acid have been determined. RESULTS: Except methanol, all other extracts exhibited antioxidative action in terms of free radical scavenging activity (FRSA) with a half-inhibiting concentration (IC50) value of 22.86 to 25.0 µg/mL, nitric oxide radical scavenging activity (NORSA) 141.3 to 149.1 µg/mL and for lipid peroxidation inhibition (LPI) 125 to 157.9 µg/mL. Usnic acid or psoromic acid showed antioxidative action with IC50 values ranging from 0.174 to 0.271 mg/mL. Methanol and ethyl acetate extract showed hydroxy-3-methyl-glutaryl-CoA reductase (HMGR) inhibition of 65.18 to 74.81%. Only 43.47% inhibition of angiotensin converting enzyme (ACE) was shown by methanol extract. Usnic acid showed noncompetitive type of HMGR inhibition and uncompetitive type of ACE inhibition. Psoromic acid exhibited competitive type of HMGR inhibition and mixed type of ACE inhibition. DISCUSSION: U. complanata showed both cardiovascular-protective and antioxidant properties. The lichen species U. complanata may be a natural bioresource for possible pharmaceutical applications.


Subject(s)
Angiotensin-Converting Enzyme Inhibitors/pharmacology , Antioxidants/pharmacology , Benzofurans/pharmacology , Benzoxepins/pharmacology , Carboxylic Acids/pharmacology , Drug Discovery , Hydroxymethylglutaryl-CoA Reductase Inhibitors/pharmacology , Usnea/metabolism , Angiotensin-Converting Enzyme Inhibitors/chemistry , Angiotensin-Converting Enzyme Inhibitors/isolation & purification , Angiotensin-Converting Enzyme Inhibitors/metabolism , Antioxidants/chemistry , Antioxidants/isolation & purification , Antioxidants/metabolism , Benzofurans/chemistry , Benzofurans/isolation & purification , Benzofurans/metabolism , Benzoxepins/chemistry , Benzoxepins/isolation & purification , Benzoxepins/metabolism , Biological Products/chemistry , Biological Products/isolation & purification , Biological Products/metabolism , Biological Products/pharmacology , Bioreactors , Carboxylic Acids/chemistry , Carboxylic Acids/isolation & purification , Carboxylic Acids/metabolism , Culture Media, Conditioned/chemistry , Culture Media, Conditioned/metabolism , Ethnopharmacology , Fermentation , Free Radical Scavengers/chemistry , Free Radical Scavengers/isolation & purification , Free Radical Scavengers/metabolism , Free Radical Scavengers/pharmacology , Hydroxymethylglutaryl-CoA Reductase Inhibitors/chemistry , Hydroxymethylglutaryl-CoA Reductase Inhibitors/isolation & purification , Hydroxymethylglutaryl-CoA Reductase Inhibitors/metabolism , India , Kinetics , Lipid Peroxidation/drug effects , Nitric Oxide/antagonists & inhibitors , Osmolar Concentration , Solubility , Solvents/chemistry , Usnea/growth & development
15.
Environ Monit Assess ; 184(1): 527-38, 2012 Jan.
Article in English | MEDLINE | ID: mdl-21409357

ABSTRACT

Lichen, Usnea barbata, transplants taken from Tierra del Fuego (south Patagonia, Argentina) were tested as potential biomonitors of atmospheric airborne deposition in an apparently pristine environment. In 2005, lichens were sampled in a reference site (n = 31) and transplanted in the northern Region of Tierra del Fuego. After, respectively, 1 month and 1 year of exposure, we collected them. The aim of the study was to determine the bioaccumulation of 26 elements in order to evaluate the background levels in the selected area. Samples were analyzed by the sector field inductively coupled plasma mass spectrometry. Discriminant analysis on principal component analysis factors was applied in order to explore the relationship among the different elements as far as time and spatial variation in transplants regards. The analysis was tested by Monte Carlo test based on 999 replicates. The most important contamination source resulted to be the atmospheric soil particle deposition. Furthermore, the results were compared with those obtained from the lichens collected in central and southern Tierra del Fuego. This study confirms the ability of U. barbata to reflect the background levels of the 26 elements in that environment. Compared with other background sites in the world, we did confirm that Tierra del Fuego lichens have a low content of the studied elements. Tierra del Fuego turned out not to be a pristine environment as supposed, but it can be considered as a reference basal ecosystem for useful comparisons among different geographical areas. These findings can be very relevant and useful for environmental conservation programs.


Subject(s)
Air Pollutants/chemistry , Environmental Monitoring/methods , Trace Elements/chemistry , Usnea/metabolism , Argentina , Atmosphere
16.
Arch Environ Contam Toxicol ; 53(4): 533-40, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17882471

ABSTRACT

In the present study, the effect of nickel incorporation on the chemical response of Ramalina celastri (Spreng.) Krog & Swinsc. and Usnea amblyoclada (Müll. Arg.) Zahlbr. exposed to urban atmospheric pollutants was assessed. Lichen thalli were treated with Ni solutions and then transplanted to two areas of the city of Córdoba with different levels of pollution. After exposure, the concentration of chlorophyll a, chlorophyll b, phaeophytin a, hydroperoxy conjugated dienes, malondialdehyde, sulphur content, electric conductivity of the immersion water, and nickel content were measured. Also, a Pollution Index was calculated for each lichen species. The exposure to Ni altered the physiological response to atmospheric pollutants in both lichen species, making them more sensitive to any damage caused by them. When the species were compared, it was observed that R. celastri accumulated more sulphur than U. amblyoclada.


Subject(s)
Air Pollutants/toxicity , Lichens/drug effects , Nickel/toxicity , Usnea/drug effects , Lichens/metabolism , Nickel/metabolism , Usnea/metabolism
17.
Ecotoxicol Environ Saf ; 67(1): 59-66, 2007 May.
Article in English | MEDLINE | ID: mdl-16870253

ABSTRACT

The effect of Cu2+, Ni2+, Pb2+, and Zn2+ on some physiological parameters of the lichen Usnea amblyoclada and the selective uptake of Cu2+ and Pb2+ was assessed. Fresh thalli were soaked in single or mixed metallic solutions. The concentration of chlorophylls and malondialdehyde; the dry weight/fresh weight ratio as well as the water content and the concentration of Cu, Ni, Pb, and Zn were measured in the treated and control thalli. The exposure to Cu, Ni, and Pb solutions caused several changes on the parameters measured; no differences were found with Zn. A stronger ability for binding Pb2+ was also observed. The results suggest that Cu2+ was the most harmful cation followed by Pb and Ni. Consequently, the damage observed in U. amblyoclada thalli when it is used as a biomonitor in polluted areas is possibly due to the presence of these heavy metals, masking the effect of other gaseous pollutants.


Subject(s)
Air Pollutants/toxicity , Metals, Heavy/toxicity , Usnea/drug effects , Air Pollutants/metabolism , Binding, Competitive , Chlorophyll/metabolism , Copper/toxicity , Environmental Monitoring/methods , Lead/toxicity , Malondialdehyde/metabolism , Metals, Heavy/metabolism , Nickel/toxicity , Solutions , Usnea/metabolism , Water/metabolism , Zinc/toxicity
18.
Microbiol Res ; 161(3): 232-7, 2006.
Article in English | MEDLINE | ID: mdl-16765839

ABSTRACT

The study was aimed to optimize the culture conditions for the production of usnic acid in the cultured cell aggregates composed of symbionts in lichen Usnea ghattensis in vitro. The cultured lichen tissue composed of symbionts appeared after about 2-3 weeks of inoculation in water-agar and malt-yeast extract (MYE) media and shown the production of usnic acid after 2-3 months of inoculation. However, the growth of symbionts was strongly affected by different culture conditions. The addition of excess carbon and nitrogen sources in the media has significantly enhanced the growth as well as usnic acid content. The cultured symbionts in MYE medium having 4% sucrose, 4% polyethyl glycol (PEG) gave 7.63 g dry biomass with 3.9 microg usnic acid/g dry biomass. In water-agar medium having 4% sucrose and 4% PEG gave 3.08 g dry biomass with 1.11 microg usnic acid/g dry biomass. The positive effects of medium on the growth of symbionts and the production of usnic acid are seemed to be due to nutritional factors.


Subject(s)
Benzofurans/metabolism , Usnea/growth & development , Usnea/metabolism , Culture Media , Polyethylene Glycols , Sucrose , Symbiosis , Time Factors , Usnea/physiology
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