Kinetics of polymorphonuclear leukocytes in an experimental hypopyon model.

Regarding the process of uveitis development, many past studies have used the experimental autoimmune uveoretinitis (EAU) and other animal models to observe histologically the infiltration of inflammatory cells and the process of lesion progression. However, no detailed study of the process of clearance of infiltrated inflammatory cells from the eye has been reported. The purpose of this study was to investigate the process of clearance of polymorphonuclear leukocytes (PMNs) using an experimental hypopyon model. PMNs obtained from ascites of SD rat were injected into the anterior chamber of SD rats. The process of PMNs clearance was evaluated by serial photography and 3D optical coherence tomography (3D-OCT), and histological changes were observed simultaneously. The hypopyon heights regressed from 1.04±0.06 mm at 1h (day 0) to 0.45±0.07 mm at day 1, and 0 mm at day 3 after PMNs injection. When the hypopyon heights at the three time points were compared, significant differences were found between groups (P<0.05). The hypopyon volumes also decreased from 1.46±0.07 mm(3) at 1h to 1.16±0.09 mm(3) at 2h, and 0.83±0.04 mm(3) at 3h after PMN injection. When the hypopyon volumes at the three time points were compared, significant differences were found between groups (P<0.05). Light micrographs of inferior segment of the eyeball revealed dense PMNs in the chamber angle at 1h after PMNs injection and many PMNs in the iris stroma and vessels, as well as at the episcleral and subconjunctival tissues around limbus at 3h and day 1 after PMNs injection. Light micrographs of superior segment of the eyeball at 3h after injection revealed PMNs in the episcleral and subconjunctival vessels. Electron micrographs of inferior segment of the eyeball at 3h after PMNs injection revealed dense PMNs with slightly condensed nuclei in the anterior chamber, as well as in the iris stroma and vessels. In conclusion, in the experimental hypopyon model, PMNs injected into the anterior chamber were cleared from the eye mainly through the iris stroma and vessels, as well as the episcleral and subconjunctival tissues around limbus.

[Effect of treatment with perftoran on the course of experimental uveitis, lipid peroxidation and activity of antioxidant defense enzymes]. / Techenie éksperimental'nogo uveita, protsessy perekisnogo okisleniia lipidov i aktivnost' fermentov antioksidantnoi zashchity na fone lecheniia perftoranom.

Experimental uveitis is characterized by a pronounced lipid peroxidation (LPO) in damaged tissues of eye, which is complicated by reduced activity of the antioxidant enzymes, SOD and catalase. The intensification of LPO was also found in blood serum and hepatic tissue. However, hepatic lipid peroxidation was accompanied by compensatory increase of catalase activity. The activity of antioxidant defence enzymes decreased in blood, whereas catalase was activated in hepatic tissue. The therapeutic effect was accompanied by a decrease of LPO products and increase of activity of the antioxidant enzymes in all tissues. Treatment of uveitis with gentamycin and, especially, perftoran reduced inflammatory events.