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High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates.

Vorimore, Fabien; Cavanna, Noémie; Vicari, Nadia; Magnino, Simone; Willems, Hermann; Rodolakis, Annie; Siarkou, Victoria I; Laroucau, Karine.

J Microbiol Methods ; 90(3): 241-4, 2012 Sep.

Article in English | MEDLINE | ID: mdl-22652433

ABSTRACT

We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.

Subject(s)

Bacterial Vaccines/genetics , Cattle Diseases/microbiology , Chlamydia Infections/veterinary , Chlamydia/genetics , Goat Diseases/microbiology , Sheep Diseases/microbiology , Animals , Bacterial Vaccines/immunology , Bacterial Vaccines/isolation & purification , Cattle , Cattle Diseases/immunology , Cattle Diseases/prevention & control , Chlamydia/immunology , Chlamydia/isolation & purification , Chlamydia Infections/immunology , Chlamydia Infections/microbiology , Chlamydia Infections/prevention & control , DNA, Bacterial/genetics , Goat Diseases/immunology , Goat Diseases/prevention & control , Goats , Molecular Typing , Polymerase Chain Reaction , Polymorphism, Single Nucleotide , Sheep , Sheep Diseases/immunology , Sheep Diseases/prevention & control , Transition Temperature , Vaccines, Live, Unattenuated/genetics , Vaccines, Live, Unattenuated/immunology , Vaccines, Live, Unattenuated/isolation & purification

ABSTRACT

Subject(s)

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