ABSTRACT
Filifactor alocis and Dialister pneumosintes have been associated with the initiation and progression of periodontitis (PE). We determined and compared the frequency of both bacteria in patients with PE, rheumatoid arthritis (RA), and PE/RA simultaneously. Detection was performed by polymerase chain reaction in the subgingival biofilm. Bacteria were more frequent in patients with PE, and clinical periodontal parameters such as pocket depth (PD) and clinical attachment loss (CAL) were significantly higher in patients with PE/RA. F. alocis and D. pneumosintes could influence PD and CAL, hence participating in the initiation and progression of PE in patients with RA.
Subject(s)
Arthritis, Rheumatoid/microbiology , Clostridiales/pathogenicity , Periodontitis/microbiology , Veillonellaceae/pathogenicity , Adult , Arthritis, Rheumatoid/drug therapy , Biofilms , Humans , Mexico , Middle Aged , Periodontitis/drug therapySubject(s)
Bartholin's Glands/microbiology , Gram-Negative Bacterial Infections/microbiology , Veillonellaceae/isolation & purification , Vulvitis/microbiology , Abscess , Adult , Amoxicillin-Potassium Clavulanate Combination/therapeutic use , Anti-Bacterial Agents/therapeutic use , Drug Resistance, Microbial , Female , Gram-Negative Bacterial Infections/drug therapy , Humans , Metronidazole/pharmacology , Veillonellaceae/drug effects , Veillonellaceae/pathogenicity , Vulvitis/drug therapyABSTRACT
Objective The aim of this study was to evaluate the association of Porphyromonas endodontalis, Filifactor alocis and Dialister pneumosintes with the occurrence of periodontitis. Material and Methods Thirty subjects with chronic periodontitis (ChP) and 10 with periodontal health (PH) were included in the study. Nine subgingival biofilm samples were collected as follows: i) PH group - from the mesial/buccal aspect of each tooth in two randomly chosen contralateral quadrants; ii) ChP group - from three sites in each of the following probing depth (PD) categories: shallow (≤3 mm), moderate (4-6 mm) and deep (≥7 mm). Checkerboard DNA-DNA hybridization was used to analyze the samples. Results We found the three species evaluated in a higher percentage of sites and at higher levels in the group with ChP than in the PH group (p<0.05, Mann-Whitney test). We also observed these differences when the samples from sites with PD≤4 mm or ≥5 mm of subjects with ChP were compared with those from subjects with PH (p<0.05, Mann-Whitney test). In addition, the prevalence and levels of D. pneumosintes, and especially of F. alocis were very low in healthy subjects (0.12x105 and 0.01x105, respectively). Conclusion F. alocis and D. pneumosintes might be associated with the etiology of ChP, and their role in the onset and progression of this infection should be further investigated. The role of P. endodontalis was less evident, since this species was found in relatively high levels and prevalence in the PH group.
Subject(s)
Chronic Periodontitis/microbiology , Peptostreptococcus/pathogenicity , Porphyromonas endodontalis/pathogenicity , Veillonellaceae/pathogenicity , Adult , Biofilms , Brazil , Case-Control Studies , Colony Count, Microbial , DNA Probes , DNA, Bacterial , Dental Plaque/microbiology , Female , Gingiva/microbiology , Humans , Male , Middle Aged , Peptostreptococcus/isolation & purification , Porphyromonas endodontalis/isolation & purification , Statistics, Nonparametric , Veillonellaceae/isolation & purificationABSTRACT
ABSTRACT Objective The aim of this study was to evaluate the association of Porphyromonas endodontalis, Filifactor alocis and Dialister pneumosintes with the occurrence of periodontitis. Material and Methods Thirty subjects with chronic periodontitis (ChP) and 10 with periodontal health (PH) were included in the study. Nine subgingival biofilm samples were collected as follows: i) PH group - from the mesial/buccal aspect of each tooth in two randomly chosen contralateral quadrants; ii) ChP group - from three sites in each of the following probing depth (PD) categories: shallow (≤3 mm), moderate (4-6 mm) and deep (≥7 mm). Checkerboard DNA-DNA hybridization was used to analyze the samples. Results We found the three species evaluated in a higher percentage of sites and at higher levels in the group with ChP than in the PH group (p<0.05, Mann-Whitney test). We also observed these differences when the samples from sites with PD≤4 mm or ≥5 mm of subjects with ChP were compared with those from subjects with PH (p<0.05, Mann-Whitney test). In addition, the prevalence and levels of D. pneumosintes, and especially of F. alocis were very low in healthy subjects (0.12x105 and 0.01x105, respectively). Conclusion F. alocis and D. pneumosintes might be associated with the etiology of ChP, and their role in the onset and progression of this infection should be further investigated. The role of P. endodontalis was less evident, since this species was found in relatively high levels and prevalence in the PH group.
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Peptostreptococcus/pathogenicity , Porphyromonas endodontalis/pathogenicity , Veillonellaceae/pathogenicity , Chronic Periodontitis/microbiology , Peptostreptococcus/isolation & purification , Brazil , DNA, Bacterial , Colony Count, Microbial , DNA Probes , Case-Control Studies , Statistics, Nonparametric , Biofilms , Porphyromonas endodontalis/isolation & purification , Dental Plaque/microbiology , Veillonellaceae/isolation & purification , Gingiva/microbiologyABSTRACT
In this report, we review one case of bacteremia infection due to Dialister pneumosintes. The patient was admitted in post-partum with vaginosis and suppurative thrombosis of the ovarian veina. D. pneumosintes was isolated in pure culture from the three blood culture flasks. Identification of this bacterium was difficult and requires the amplification and partial sequencing of the 16S rRNA gene. The patients had favorable outcome after antibiotic treatment.
