ABSTRACT
Prevention of auditory hair cell death offers therapeutic potential to rescue hearing. Pharmacological blockade of JNK/c-Jun signaling attenuates injury-induced hair cell loss, but with unsolved mechanisms. We have characterized the c-Jun stress response in the mouse cochlea challenged with acoustic overstimulation and ototoxins, by studying the dynamics of c-Jun N-terminal phosphorylation. It occurred acutely in glial-like supporting cells, inner hair cells, and the cells of the cochlear ion trafficking route, and was rapidly downregulated after exposures. Notably, death-prone outer hair cells lacked c-Jun phosphorylation. As phosphorylation was triggered also by nontraumatic noise levels and none of the cells showing this activation were lost, c-Jun phosphorylation is a biomarker for cochlear stress rather than an indicator of a death-prone fate of hair cells. Preconditioning with a mild noise exposure before a stronger traumatizing noise exposure attenuated the cochlear c-Jun stress response, suggesting that the known protective effect of sound preconditioning on hearing is linked to suppression of c-Jun activation. Finally, mice with mutations in the c-Jun N-terminal phosphoacceptor sites showed partial, but significant, hair cell protection. These data identify the c-Jun stress response as a paracrine mechanism that mediates outer hair cell death.
Subject(s)
Biomarkers/metabolism , Hair Cells, Vestibular/metabolism , Hearing Loss, Noise-Induced/pathology , JNK Mitogen-Activated Protein Kinases/metabolism , Vestibulocochlear Nerve Injuries/pathology , Animals , Animals, Newborn , Apoptosis , Cell Death/drug effects , Cell Death/physiology , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Disease Models, Animal , Female , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Kanamycin/toxicity , Male , Mice , Mice, Inbred CBA , Mice, Transgenic , Noise/adverse effects , Protein Synthesis Inhibitors/toxicity , Time Factors , Transcription Factors/genetics , Transcription Factors/metabolism , Vestibulocochlear Nerve Injuries/chemically inducedABSTRACT
Ouabain application to the round window can selectively destroy type-I spiral ganglion cells, producing an animal model of auditory neuropathy. To assess the long-term effects of this deafferentation on synaptic organization in the organ of Corti and cochlear nucleus, and to ask whether surviving cochlear neurons show any post-injury plasticity in the adult, we quantified the peripheral and central synapses of type-I neurons at posttreatment times ranging from 1 to 3 months. Measures of normal DPOAEs and greatly reduced auditory brainstem responses (ABRs) confirmed the neuropathy phenotype. Counts of presynaptic ribbons and postsynaptic glutamate receptor patches in the inner hair cell area decreased with post-exposure time, as did counts of cochlear nerve terminals in the cochlear nucleus. Although these counts provided no evidence of new synapse formation via branching from surviving neurons, the regular appearance of ectopic neurons in the inner hair cell area suggested that neurite extension is not uncommon. Correlations between pathophysiology and histopathology showed that ABR thresholds are very insensitive to even massive neural degeneration, whereas the amplitude of ABR wave 1 is a better metric of synaptic degeneration.
Subject(s)
Cochlear Nerve/pathology , Nerve Degeneration/chemically induced , Neuronal Plasticity/drug effects , Ouabain/adverse effects , Ouabain/pharmacology , Synapses/drug effects , Vestibulocochlear Nerve Injuries/chemically induced , Animals , Cochlea/drug effects , Cochlea/innervation , Cochlea/physiopathology , Cochlear Nerve/drug effects , Disease Models, Animal , Enzyme Inhibitors/adverse effects , Enzyme Inhibitors/pharmacology , Female , Mice , Mice, Inbred CBA , Nerve Degeneration/pathology , Nerve Degeneration/physiopathology , Organ of Corti/pathology , Organ of Corti/physiopathology , Presynaptic Terminals/drug effects , Presynaptic Terminals/parasitology , Receptors, Glutamate/drug effects , Receptors, Glutamate/physiology , Synapses/pathology , Time Factors , Vestibulocochlear Nerve Injuries/pathology , Vestibulocochlear Nerve Injuries/physiopathologyABSTRACT
OBJECTIVE: The degeneration of hair cells and spiral ganglion neurons (SGNs) is an important pathologic process in the development of sensorineural hearing loss. In a murine model, predictable and reproducible damage to SGNs occurs through the application of ouabain to the round window. Recent evidence has shown that the chemokine stromal cell-derived factor-1 (SDF-1) is a potent chemoattractant of hematopoietic stem cells (HSCs) and provides trophic support to injured tissues during development and maturation. The hypothesis for the current study is that expression of SDF-1 plays an important role in protecting SGNs and preventing further degeneration in the setting of cochlear injury. STUDY DESIGN: Prospective, controlled. SETTING: Academic research laboratory. SUBJECT AND METHODS: Auditory brainstem response (ABR) and the expression of SDF-1 mRNA and protein were examined 1, 3, 7, 14, and 30 days after application of ouabain in 35 adult mice. RESULTS: Following ouabain application, real-time reverse-transcription polymerase chain reaction for SDF demonstrates increased mRNA expression following ouabain injury in nontransplanted mice. A significant increase in SDF protein expression was also observed using immunolabeling techniques and Western blot analysis. CONCLUSIONS: SDF-1 expression is increased in the auditory nerve following cochlear injury. Further knowledge about the cochlear microenvironment, including SDF-1, is critical to maximizing HSC engraftment in the injured cochlea and providing a therapeutic option for sensorineural hearing loss.
