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1.
PLoS One ; 14(6): e0217474, 2019.
Article in English | MEDLINE | ID: mdl-31170178

ABSTRACT

Hand, foot, and mouth disease (HFMD) is well recognized as one of the major threats to children's health globally. The increasing complexity of the etiology of HFMD still challenges disease control in China. There is little surveillance of the molecular epidemiological characteristics of the enteroviruses (EVs) that cause HFMD in Neijiang city or the Sichuan Basin area in Southwest China. In this study, demographic and epidemiological information for 14,928 probable HFMD cases was extracted and analyzed to describe the epidemic features of HFMD in Neijiang city from Jan 2010 to Dec 2017. The swab samples of select probable HFMD cases from 2012 to 2017 were tested by reverse transcription (RT) real-time PCR to identify the serotype distribution of EVs, and 110 randomly selected RT-real-time PCR positive samples were then amplified and analyzed for the VP1 or VP4 regions of EVs to further analyze the phylogenetic characteristics of the circulating strains in this area. The eight-year average annual incidence was 49.82 per 100,000 in Neijiang. The incidence rates varied between 19.51 and 70.73 per 100,000, demonstrating peaks of incidence in even-number years (2012, 2014 and 2016). The median age of the probable cases was 27 months and the interquartile range (25th to 75th percentile) of ages for the probable HFMD cases was between 14 and 42 months. The male-to-female ratio of the probable HFMD cases was 1.47:1, and scattered children were the major population classification (81.7%). Two epidemic peaks were observed: one major peak between April and July and the other lesser peak between October and December. Of 6513 probable cases tested with RT-real-time PCR, 4015 (61.6%) were positive for enterovirus with the serotype distribution as follows: EV71+, 30.1% (n = 1210); CV-A16+, 28.7% (n = 1154) and a sole pan-enterovirus+, 41.1% (n = 1651). A total of 91 cases (82.7%, 91/110) were successfully amplified and underwent phylogenetic analysis: all EV71+ cases were C4a serotype (n = 23/30); all CV-A16+ cases were B2b serotype (n = 24/30); of 42 sole pan-enterovirus+ samples, 20 were CV-A6, 14 were CV-A10 and the rest within this group were CV-A4 (n = 4), CV-A8 (n = 2), CV-A9 (n = 1) and CV-B3 (n = 1). Our findings provide important evidence that aids the improvement of strategies for vaccination against HFMD and comprehensive disease control in China.


Subject(s)
Capsid Proteins , Enterovirus B, Human/genetics , Hand, Foot and Mouth Disease , Phylogeny , Serogroup , Viral Structural Proteins , Capsid Proteins/blood , Capsid Proteins/genetics , Child, Preschool , China/epidemiology , Female , Hand, Foot and Mouth Disease/blood , Hand, Foot and Mouth Disease/epidemiology , Hand, Foot and Mouth Disease/genetics , Hand, Foot and Mouth Disease/virology , Humans , Incidence , Infant , Male , Retrospective Studies , Reverse Transcriptase Polymerase Chain Reaction , Seroepidemiologic Studies , Viral Structural Proteins/blood , Viral Structural Proteins/genetics
2.
PLoS Pathog ; 8(7): e1002826, 2012.
Article in English | MEDLINE | ID: mdl-22910880

ABSTRACT

Enterovirus 71 (EV71) is one of the most virulent enteroviruses, but the specific molecular features that enhance its ability to disseminate in humans remain unknown. We analyzed the genomic features of EV71 in an immunocompromised host with disseminated disease according to the different sites of infection. Comparison of five full-length genomes sequenced directly from respiratory, gastrointestinal, nervous system, and blood specimens revealed three nucleotide changes that occurred within a five-day period: a non-conservative amino acid change in VP1 located within the BC loop (L97R), a region considered as an immunogenic site and possibly important in poliovirus host adaptation; a conservative amino acid substitution in protein 2B (A38V); and a silent mutation in protein 3D (L175). Infectious clones were constructed using both BrCr (lineage A) and the clinical strain (lineage C) backgrounds containing either one or both non-synonymous mutations. In vitro cell tropism and competition assays revealed that the VP197 Leu to Arg substitution within the BC loop conferred a replicative advantage in SH-SY5Y cells of neuroblastoma origin. Interestingly, this mutation was frequently associated in vitro with a second non-conservative mutation (E167G or E167A) in the VP1 EF loop in neuroblastoma cells. Comparative models of these EV71 VP1 variants were built to determine how the substitutions might affect VP1 structure and/or interactions with host cells and suggest that, while no significant structural changes were observed, the substitutions may alter interactions with host cell receptors. Taken together, our results show that the VP1 BC loop region of EV71 plays a critical role in cell tropism independent of EV71 lineage and, thus, may have contributed to dissemination and neurotropism in the immunocompromised patient.


Subject(s)
Enterovirus A, Human/physiology , Enterovirus A, Human/pathogenicity , Enterovirus Infections/virology , Neurons/virology , Viral Structural Proteins/genetics , Virus Attachment , Adult , Amino Acid Substitution , Animals , Bronchoalveolar Lavage Fluid/virology , Caco-2 Cells , Cell Line , Cell Line, Tumor , Chlorocebus aethiops , DNA, Viral/genetics , Enterovirus A, Human/genetics , Enterovirus A, Human/immunology , Feces/virology , Humans , Immunocompromised Host , Male , Mutation , Neuroblastoma , Vero Cells , Viral Structural Proteins/blood , Viral Structural Proteins/cerebrospinal fluid , Viral Structural Proteins/immunology
3.
Vopr Virusol ; 56(2): 28-32, 2011.
Article in Russian | MEDLINE | ID: mdl-21545038

ABSTRACT

Institute of Medical Primatology, Russian Academy of Medical Sciences, Sochi The conserved regions of nucleotide sequences were found in primate cytomegaloviruses (CMV). Universal primers were designed for the consensus sequence of a conservative region of the UL56 gene of the betaherpesvirinae subfamily. Amplification, sequencing, and phylogenetic analysis of the fragments of CMV strains isolated from man and different primate species were made. Analysis of sequenced gene fragments showed that the UL56 gene area is most suitable for the phylogenetic analysis of primate CMV and could identify several groups of clusters by the degree of relationship among the viruses of this family.


Subject(s)
Consensus Sequence/genetics , Cytomegalovirus/genetics , DNA Primers/genetics , DNA, Viral/genetics , Phylogeny , Animals , Base Sequence , Cercopithecinae , Cytomegalovirus/isolation & purification , Humans , Molecular Sequence Data , Polymerase Chain Reaction , Salivary Glands/chemistry , Sequence Alignment , Software , Viral Structural Proteins/blood
4.
J Med Virol ; 83(7): 1134-41, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21520140

ABSTRACT

Hepatitis A virus (HAV) is usually transmitted by an oral-fecal route and is prevalent not only in developing countries but also in developed countries. In the present study, the phylogenetic characterization of the VP1/2A junction region (321 nucleotides) of China HAV isolates was examined. Anti-HAV IgM-positive serum samples were collected from 8 provinces, including 20 cities or counties in China from 2003 to 2008; 337 isolates from 406 HAV patients' serum samples were amplified by RT-PCR, sequenced at the VP1/2A junction region and aligned with the published sequences from GenBank to establish phylogenetic analysis. All China HAV isolates in this study belonged to genotype I, with 98.8% (333/337) of samples clustering in sub-genotype IA and 1.2% (4/337) in sub-genotype IB. In addition, sub-genotype IA isolates clustered into four groups (92.7-100% nucleotide identity), and the samples collected from all China HAV isolates in this investigation showed 87.5-100% nucleotide identity, but the amino acids in this region were more conserved (95.2-100% identity). Few unique amino acid changes could be deduced (VP1-253: Glu → Gly; 2A-34: Pro → Ala; 2A-33: Leu → Phe). Genetically identical or similar HAV strains existed in some investigated areas in China during different years, suggesting that an indigenous strain has been circulating in those regions. This report provides new data on the genetic relatedness and molecular epidemiology of HAV isolates from China as well as the distribution of sub-genotype IA and IB in this part of the world.


Subject(s)
Cysteine Endopeptidases/genetics , Hepatitis A Virus, Human/classification , Hepatitis A Virus, Human/genetics , Hepatitis A/genetics , RNA, Viral/genetics , Viral Proteins/genetics , Viral Structural Proteins/genetics , Amino Acid Substitution , Base Sequence , China , Cluster Analysis , Conserved Sequence , Cysteine Endopeptidases/blood , Cysteine Endopeptidases/chemistry , Databases, Genetic , Genotype , Hepatitis A/blood , Hepatitis A/epidemiology , Hepatitis A/virology , Hepatitis A Antibodies/analysis , Hepatitis A Antibodies/genetics , Hepatitis A Virus, Human/immunology , Hepatitis A Virus, Human/isolation & purification , Humans , Molecular Sequence Data , Molecular Typing , Phylogeny , RNA, Viral/analysis , RNA, Viral/blood , Retrospective Studies , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNA , Viral Proteins/blood , Viral Proteins/chemistry , Viral Structural Proteins/blood , Viral Structural Proteins/chemistry
5.
J Med Virol ; 82(8): 1400-5, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20578057

ABSTRACT

The fact that the diagnosis of infection with dengue virus is usually made by detecting IgM antibodies during the convalescent phase of the disease interferes with disease management and, consequently, with reducing mortality rates. This study evaluated the sensitivity and specificity of detection of NS1 in samples of patients suspected of acute dengue virus infection in Brazil. The results were used to institute treatment and the sensitivity and specificity of detection of NS1 were compared to the results of detection of IgM, virus isolation, and RT-PCR. Detection of NS1 yielded better results than RT-PCR and virus isolation. When considering IgM detection and RT-PCR positive results as "gold standards," the sensitivity and specificity of the NS1 assay were 95.9% and 81.1%, respectively. All patients enrolled in the study were treated promptly and had an uneventful course of the disease. The detection of NS1 provided better results than the diagnostic techniques used currently during the acute phase of disease (RT-PCR and virus isolation). Detection of NS1 is an important tool for the diagnosis of acute dengue infection, particularly in highly endemic areas, allowing for rapid treatment of patients and reduction of disease burden.


Subject(s)
Dengue Virus/isolation & purification , Dengue/diagnosis , Viral Structural Proteins/blood , Virology/methods , Adult , Antibodies, Viral/blood , Brazil , Early Diagnosis , Enzyme-Linked Immunosorbent Assay/methods , Female , Humans , Immunoglobulin M/blood , Male , Middle Aged , RNA, Viral/blood , Reverse Transcriptase Polymerase Chain Reaction/methods , Sensitivity and Specificity
6.
J Virol Methods ; 66(2): 211-8, 1997 Jul.
Article in English | MEDLINE | ID: mdl-9255732

ABSTRACT

An improved solid-phase enzyme linked immunosorbent assay (ELISA) using monoclonal antibodies was developed to detect an African swine fever virus protein (VP73) in pig samples. The use of monoclonal antibodies against VP73 allowed a sensitive and specific sandwich ELISA. This assay detected a limiting antigen concentration of 0.05 microgram/ml of VP73, lower than the detection limit of 0.6 microgram/ml obtained by using polyclonal antibodies by the same ELISA. The whole virus particle was detected with this method to a limit of 2.3 x 10(2) PFU/ml. At the same time, an indirect ELISA was developed to detect ASFV antibodies. The results also indicate that this method may be a useful technique for epidemiological surveys.


Subject(s)
African Swine Fever Virus/immunology , African Swine Fever/diagnosis , Antibodies, Viral/blood , Antigens, Viral/blood , Enzyme-Linked Immunosorbent Assay/methods , African Swine Fever Virus/isolation & purification , Animals , Antibodies, Monoclonal , Antigens, Viral/isolation & purification , Sensitivity and Specificity , Swine , Viral Structural Proteins/blood , Viral Structural Proteins/isolation & purification
7.
AIDS Res Hum Retroviruses ; 13(1): 97-104, 1997 Jan 01.
Article in English | MEDLINE | ID: mdl-8989432

ABSTRACT

Apolipoprotein H (apo H), isolated from human plasma albumin solution, was shown to capture HIV-1-related antigens from antigen-positive sera (HIV-1 AG+) of AIDS patients, by using HIV-1-specific polyclonal antibodies. In an enzyme-linked immunosorbent assay and ligand blot and dot assays, apo H was able to bind recombinant retroviral HIV antigens, especially Gag proteins p18 of HIV-1, p26 of HIV-2, and Env gp160 of HIV-1. Binding was shown to be pH and NaCl dependent, with an optimum at acidic pH and low ionic strength. Specificity was demonstrated by saturation of this binding and inhibition either by homologous competition or by specific antisera. Binding was also observed in cell line-harvested viral proteins. The mechanism of this apo H-polyspecific binding is discussed in relation to conformational changes due to the influence of lipids or detergents.


Subject(s)
Apolipoproteins/metabolism , Glycoproteins/metabolism , HIV-1 , HIV-2 , Viral Structural Proteins/metabolism , Acquired Immunodeficiency Syndrome/virology , Apolipoproteins/blood , Apolipoproteins/chemistry , Detergents , Glycoproteins/blood , Glycoproteins/chemistry , HIV Antibodies , Humans , Hydrogen-Ion Concentration , Immune Sera , Immunoassay/methods , Octoxynol , Osmolar Concentration , Protein Binding , Serum Albumin , Viral Structural Proteins/blood , beta 2-Glycoprotein I
8.
Clin Diagn Lab Immunol ; 1(1): 5-10, 1994 Jan.
Article in English | MEDLINE | ID: mdl-7496921

ABSTRACT

To determine the serologic cross-reactivity between human T-cell lymphotropic virus type I (HTLV-I) and parasite antigens, we measured antibody responses against HTLV-I, Plasmodium falciparum, Plasmodium vivax, and Brugia malayi in serum specimens obtained from regions where malaria (n = 482) and filariasis (n = 101) are endemic. Analysis of immune reactivity to HTLV-I antigens showed that specimens from regions where malaria is endemic had significantly higher rates of enzyme immunoassay (EIA) reactivity (76 of 482 [15.8%] than those from regions where filariasis is endemic (0 of 101 [0%]). Western blot (immunoblot) analysis of the HTLV-I EIA-reactive specimens demonstrated predominant Gag reactivity (HTLV-Iind). Only two specimens each from Indonesia and Brazil and four specimens from Papua New Guinea had Env reactivity by radioimmunoprecipitation analysis. Furthermore, a positive correlation between HTLV-EIA and titers of antibody to the blood stage of P. falciparum (rs = 0.24, P < 0.005) was discerned; no correlation was observed between antibodies to the blood stage or the circumsporozoite protein of P. vivax and the circumsporozoite protein of P. falciparum. In addition, P. falciparum-infected erythrocyte lysate specifically abrogated binding of Gag-specific antibodies in HTLV-Iind specimens from regions where malaria is endemic without affecting binding in HTLV-I-seropositive specimens, suggesting that the immunologic cross-reactivity between HTLV Gag proteins and malaria parasites is restricted to the blood-stage antigens of plasmodia in specimens from regions where malaria is endemic. However, HTLV-seroindeterminate specimens from the United States did not demonstrate serologic cross-reactivity, suggesting that antigenic mimicry of HTLV proteins extends to other nonplasmodial antigens as well.


Subject(s)
Antigens, Protozoan/immunology , Malaria, Falciparum/blood , Plasmodium falciparum/immunology , Viral Structural Proteins/immunology , Animals , Antigens, Protozoan/blood , Binding Sites, Antibody , Binding, Competitive/immunology , Cross Reactions , Erythrocytes/immunology , HTLV-I Antibodies/blood , Humans , Malaria, Falciparum/immunology , Malaria, Falciparum/parasitology , Plasmodium falciparum/growth & development , Viral Structural Proteins/blood
9.
Am J Vet Res ; 51(10): 1540-3, 1990 Oct.
Article in English | MEDLINE | ID: mdl-2240773

ABSTRACT

Two African swine fever virus (ASFV) antigens were tested for use in an ELISA to detect antibody to ASFV. Antigens used were the cytoplasmic soluble fraction (CS-P) of infected cells grown in the presence of porcine serum and the semipurified viral structural protein VP73 (SVP73). Both antigens were tested by ELISA against 72 sera obtained during several ASF field episodes and from ASFV-inapparent carriers. Of the 72 sera, only 2.8% has positive results by ELISA against CS-P antigen; 60% of positive-reacting sera (to both antigens) had higher ELISA values when the CS-P antigen was used. Samples (with positive results) that reacted only to CS-P antigen had results confirmed by immunoblot analysis. Such sera reacted against ASFV-infection proteins IP25, IP25.5, and IP30, but not against IP73. In time-course experiments to detect appearance of ASFV-antibodies in infected miniature pigs, antibodies were detected by immunoblot analysis on postinoculation day (PID) 8. At that time, only the polypeptides IP25, IP25.5 IP30, and IP31 were recognized; IP73 and IP12 were first detected 3 and 4 days later, respectively. In the same experiments, ASFV antibodies were detected by ELISA, using CS-P or SVP73 antigens, on PID 7 and 9, respectively. These results could explain the percentage of sera not having positive results by ELISA using SVP73 antigen, if the sera were obtained from ASFV-infected pigs during the first days of infection before induction of antibody response against the IP73 protein. This feature makes the use of CS-P antigen advantageous in early serologic detection of ASFV-infected pigs.


Subject(s)
African Swine Fever Virus/immunology , Antibodies, Viral/blood , Antigens, Viral , Swine, Miniature/immunology , Viral Proteins/immunology , Viral Structural Proteins , Animals , Antibodies, Viral/immunology , Antigens, Viral/immunology , Enzyme-Linked Immunosorbent Assay/veterinary , Immunoblotting/veterinary , Swine , Swine, Miniature/blood , Viral Proteins/blood , Viral Structural Proteins/blood
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