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1.
Tissue Cell ; 72: 101531, 2021 Oct.
Article in English | MEDLINE | ID: mdl-33798831

ABSTRACT

Three POU family class V gene homologues are expressed in the development of Xenopus. In contrast to the expression of Pou5f3.1 and Pou5f3.2 in organogenesis, Pou5f3.3 is expressed during oogenesis in ovary. We investigated the expression and function of Pou5f3.3 in organogenesis of Xenopus laevis. RT-PCR and immunohistochemical analysis indicated that Pou5f3.3 was expressed in a small number of adult liver cells and blood cells. Immunocytochemical investigation proved that Bmi1, a marker for hematopoietic progenitor cells, was co-expressed in Pou5f3.3-expressing small spherical cells in the peripheral blood. In anemic induction by intraperitoneal injection of phenyl hydrazine, the number of Pou5f3.3-expressing cells significantly increased within 3 days after phenyl hydrazine injection. In CRISPR/Cas mutagenesis of Pou5f3.3, Bmi1-positive hematopoietic progenitor cell count decreased in the hematopoietic dorsal-lateral plate (DLP) region, resulting in a considerable reduction in peripheral blood cells. CRISPR/Cas-induced hematopoietic deficiency was completely rescued by Pou5f3.3 supplementation, but not by Pou5f3.1 or Pou5f3.2. Transplantation experiments using the H2B-GFP transgenic line demonstrated that DLP-derived Pou5f3.3-positive and Bmi1-positive cells were translocated into the liver and bone through the bloodstream. These results suggest that Pou5f3.3 plays an essential role in the establishment and maintenance of hematopoietic progenitor cells during Xenopus development.


Subject(s)
Embryonic Development , Hematopoietic Stem Cells/metabolism , POU Domain Factors/metabolism , Xenopus Proteins/metabolism , Xenopus laevis/embryology , Anemia/pathology , Animals , Base Sequence , CRISPR-Cas Systems/genetics , Cell Movement , Gene Expression Regulation, Developmental , Hematopoiesis , Mutagenesis/genetics , POU Domain Factors/blood , POU Domain Factors/genetics , Xenopus Proteins/blood , Xenopus Proteins/genetics , Xenopus laevis/genetics
2.
Arch Gynecol Obstet ; 296(4): 841-846, 2017 Oct.
Article in English | MEDLINE | ID: mdl-28831586

ABSTRACT

PURPOSE: This study was conducted to compare serum xenopsin-related peptide-1 (XP-1) levels in women with polycystic ovary syndrome (PCOS) and in healthy women and to determine the role of XP-1 levels in PCOS. METHODS: Forty patients with PCOS and 38 healthy women were included in the study and matched with age and body mass index. Fasting blood glucose, insulin, high sensitivity C-reactive protein (hs-CRP), XP-1 and total testosterone levels of all participants were measured. RESULTS: Serum XP-1 levels significantly increased in women with PCOS compared to the control group (6.49 ± 1.57 vs 5.29 ± 1.45 ng/ml, p = 0.001). Serum insulin, hs-CRP, HOMA-IR, total testosterone levels and waist circumference were higher in women with PCOS than in control group. High XP-1 levels were associated with PCOS after adjustment for potential confounders. Receiver operating characteristic (ROC) curve analysis confirmed that the area under ROC curves was 0.703 (95% CI 0.588-0.818, p < 0.002) for XP-1 levels. The optimal cut-off value of XP-1 for detecting PCOS was ≥5.87 ng/ml. CONCLUSIONS: Our results indicate that increased XP-1 levels were associated with PCOS after adjustment for potential confounders, which has been shown to be effective in the function of the insulin signaling pathway.


Subject(s)
Body Mass Index , Peptides/blood , Polycystic Ovary Syndrome/blood , Polycystic Ovary Syndrome/diagnosis , Xenopus Proteins/blood , Adult , Biomarkers/blood , C-Reactive Protein/metabolism , Case-Control Studies , Female , Humans , Insulin/blood , Insulin Resistance/physiology , ROC Curve , Testosterone/blood
3.
Dev Comp Immunol ; 40(2): 94-102, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23454582

ABSTRACT

Xenopus laevis serum lectin XCL1 is a newly identified molecule of the XCGL (or X-lectin) family, a unique group of Ca(2+)-dependent lectins that have a fibrinogen-like domain. The XCL1 protein was purified from lipopolysaccharide (LPS)-stimulated frog sera by sequential affinity chromatography on heparin-acrylic beads and galactose-Sepharose. XCL1 comprises multiple oligomeric proteins consisting of 37-kDa subunit polypeptides, as revealed by sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) and Western blot analyses using the monoclonal antibody (mAb) produced against the recombinant XCL1 polypeptide. In the presence of Ca(2+), the protein bound to Escherichia coli, Staphylococcus aureus, LPS and galactose and the bound XCL1 was competitively eluted using ribose and xylose, and the elution was as efficient as that using EDTA, whereas elution using hexoses, GalNAc or GlcNAc was less effective. In reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses, XCL1 expression was ubiquitously detected in frog tissues, with relatively high levels in hematopoietic tissues including the spleen, liver and kidney. Intraperitoneal injection of E. coli, S. aureus or 100-300µg S-type LPS from various bacteria induced several-fold increases in serum XCL1 concentrations on day 3, and the elevated levels retained up to day 12. It also caused a remarkable increase of the splenic XCL1 expression on day 3, followed by a rapid decline to nearly nonstimulated control levels by day 7. The R-type LPS with shortened polysaccharide chains was less effective in inducing the serum XCL1 response, indicating that the sugar chains of LPS were important, if not essential, for the stimulation of XCL1 production. These results suggest that XCL1 is a pathogen recognition molecule involved in antimicrobial innate immunity in Xenopus.


Subject(s)
Lectins/blood , Lipopolysaccharides/pharmacology , Xenopus Proteins/blood , Xenopus laevis/immunology , Animals , Chromatography, Affinity , Escherichia coli/immunology , Gene Expression/immunology , Host-Pathogen Interactions , Immunity, Innate , Lectins/genetics , Lectins/isolation & purification , Lectins, C-Type , Organ Specificity , Protein Binding , Staphylococcus aureus/immunology , Up-Regulation/immunology , Xenopus Proteins/genetics , Xenopus Proteins/isolation & purification
4.
Br J Nutr ; 102(12): 1767-75, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19822030

ABSTRACT

The present study was planned to investigate the protective effect of 10 % and 20 % apricot-containing feed on carbon tetrachloride (CCl4)-induced hepatic steatosis and damage. Adult male Wistar rats (n 42) were divided into six groups of seven each, as follows: control group; CCl4 group; CCl4+10 % apricot group; CCl4+20 % apricot group; 10 % apricot group; 20 % apricot group. All apricot groups were fed with 10 % or 20 % apricot-containing feed for 5 months. CCl4 injections were applied to the CCl4 groups at the dose of 1 mg/kg for 3 d at the end of 5 months. In the CCl4 group, vacuolated hepatocytes and hepatic necrosis were seen, especially in the centrilobular area. Hepatocytes showed an oedematous cytoplasmic matrix, large lipid globules and degenerated organelles. The area of liver injury was found significantly decreased with apricot feeding. Malondialdehyde and total glutathione levels and catalase, superoxide dismutase and glutathione peroxidase activities were significantly changed in the CCl4 group and indicated increased oxidative stress. Apricot feeding decreased this oxidative stress and ameliorated histological damage. We concluded that apricot feeding had beneficial effects on CCl4-induced liver steatosis and damage probably due to its antioxidant nutrient (beta-carotene and vitamin) contents and high radical-scavenging capacity. Dietary intake of apricot can reduce the risk of liver steatosis and damage caused by free radicals.


Subject(s)
Fatty Liver/prevention & control , Fruit/chemistry , Plant Extracts/administration & dosage , Prunus/chemistry , Alanine Transaminase/blood , Animals , Antioxidants/administration & dosage , Antioxidants/analysis , Carbon Tetrachloride , Catalase/metabolism , Fatty Liver/chemically induced , Fatty Liver/pathology , Free Radicals , Glutathione/analysis , Glutathione Peroxidase/metabolism , Hepatocytes/ultrastructure , Lipid Peroxidation , Liver/enzymology , Liver/physiopathology , Liver/ultrastructure , Male , Microscopy, Electron, Transmission , Nuclear Proteins/blood , Oxidative Stress/drug effects , Rats , Rats, Wistar , Superoxide Dismutase/metabolism , Xenopus Proteins/blood
5.
J Clin Microbiol ; 42(6): 2419-24, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15184413

ABSTRACT

Paracoccidioidomycosis (PCM) is a systemic fungal disease that is particularly important among individuals living and working in rural areas of endemicity in Latin America. Detection of anti-Paracoccidioides brasiliensis antibodies is of limited value due to false-negative results. Detection of P. brasiliensis-gp43 circulating antigen is a practical approach for a specific diagnosis of the disease. In a previous study we described an inhibition enzyme-linked immunosorbent assay able to detect the 43-kDa P. brasiliensis antigen in sera of 100% of patients with the acute form of PCM and in 95.31 and 100% of patients with the chronic multifocal and unifocal forms of PCM. To investigate its potential application for the follow-up of PCM patients during treatment, antigen levels were monitored at regular intervals for up 8 to 12 months in serum samples from 23 patients. The results showed that treatment with itraconazole resulted in decreasing levels of circulating gp43 that were correlated with the reduction of anti-gp43 antibodies. It was also observed that by the end of 12 months of treatment gp43 levels were <5 microg/ml in all patients.


Subject(s)
Antigens, Fungal/blood , Fungal Proteins , Glycoproteins/blood , Paracoccidioidomycosis/drug therapy , Xenopus Proteins/blood , Adult , Aged , Humans , Male , Middle Aged , Paracoccidioidomycosis/immunology
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