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1.
Clin Exp Immunol ; 185(1): 98-106, 2016 07.
Article in English | MEDLINE | ID: mdl-26990648

ABSTRACT

Salmonellosis or Salmonella, one of the most common food-borne diseases, remains a major public health problem worldwide. Intestinal epithelial cells (IECs) play an essential role in the mucosal innate immunity of the host to defend against the invasion of Salmonella by interleukin (IL)-8 and human ß-defensin-2 (hBD-2). Accumulated research has unravelled important roles of vitamin D in the regulation of innate immunity. Therefore, we investigated the effects of 1,25-dihydroxyvitamin D3 (1,25D3) on Salmonella-induced innate immunity in IECs. We demonstrate that pretreatment of 1,25D3 results in suppression of Salmonella-induced IL-8 but enhancement of hBD-2, either protein secretion and mRNA expression, in IECs. Furthermore, 1,25D3 enhanced Salmonella-induced membranous recruitment of nucleotide oligomerization domain (NOD2) and its mRNA expression and activation of protein kinase B (Akt), a downstream effector of phosphoinositide 3-kinase (PI3K). Inhibition of the PI3K/Akt signal counteracted the suppressive effect of 1,25D3 on Salmonella-induced IL-8 expression, while knock-down of NOD2 by siRNA diminished the enhanced hBD-2 expression. These data suggest differential regulation of 1,25D3 on Salmonella-induced IL-8 and hBD-2 expression in IECs via PI3K/Akt signal and NOD2 protein expression, respectively. Active vitamin D-enhanced anti-microbial peptide in Salmonella-infected IECs protected the host against infection, while modulation of proinflammatory responses by active vitamin D prevented the host from the detrimental effects of overwhelming inflammation. Thus, active vitamin D-induced innate immunity in IECs enhances the host's protective mechanism, which may provide an alternative therapy for invasive Salmonella infection.


Subject(s)
Calcitriol/pharmacology , Epithelial Cells/drug effects , Host-Pathogen Interactions/drug effects , Interleukin-8/genetics , beta-Defensins/genetics , Cell Line, Tumor , Epithelial Cells/immunology , Epithelial Cells/microbiology , Gene Expression Regulation , Humans , Interleukin-8/antagonists & inhibitors , Interleukin-8/immunology , Nod2 Signaling Adaptor Protein/antagonists & inhibitors , Nod2 Signaling Adaptor Protein/genetics , Nod2 Signaling Adaptor Protein/immunology , Phosphatidylinositol 3-Kinases/genetics , Phosphatidylinositol 3-Kinases/immunology , Proto-Oncogene Proteins c-akt/genetics , Proto-Oncogene Proteins c-akt/immunology , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Salmonella typhimurium/growth & development , Salmonella typhimurium/immunology , Signal Transduction , beta-Defensins/agonists , beta-Defensins/immunology
2.
J Eukaryot Microbiol ; 62(1): 44-50, 2015.
Article in English | MEDLINE | ID: mdl-25155632

ABSTRACT

IL-18 is known to play a key role limiting Cryptosporidium parvum infection. In this study, we show that IL-18 depletion in SCID mice significantly exacerbates C. parvum infection, whereas, treatment with recombinant IL-18 (rIL-18), significantly decreases the parasite load, as compared to controls. Increases in serum IFN-γ levels as well as the up-regulation of the antimicrobial peptides, cathelicidin antimicrobial peptide and beta defensin 3 (Defb3) were observed in the intestinal mucosa of mice treated with rIL-18. In addition, C. parvum infection significantly increased mRNA expression levels (> 50 fold) of the alpha defensins, Defa3 and 5, respectively. Interestingly, we also found a decrease in mRNA expression of IL-33 (a recently identified cytokine in the same family as IL-18) in the small intestinal tissue from mice treated with rIL-18. In comparison, the respective genes were induced by IL-18 depletion. Our findings suggest that IL-18 can mediate its protective effects via different routes such as IFN-γ induction or by directly stimulating intestinal epithelial cells to increase antimicrobial activity.


Subject(s)
Cryptosporidiosis/drug therapy , Immunity, Innate/drug effects , Immunity, Mucosal/drug effects , Interleukin-18/pharmacology , Intestinal Mucosa/drug effects , RNA, Messenger/immunology , Animals , Antimicrobial Cationic Peptides , Cathelicidins/agonists , Cathelicidins/genetics , Cathelicidins/immunology , Cryptosporidiosis/immunology , Cryptosporidiosis/parasitology , Cryptosporidium parvum/immunology , Female , Gene Expression Regulation , Interferon-gamma/agonists , Interferon-gamma/genetics , Interferon-gamma/immunology , Interleukin-18/genetics , Interleukin-18/immunology , Interleukin-33/antagonists & inhibitors , Interleukin-33/genetics , Interleukin-33/immunology , Intestinal Mucosa/immunology , Intestinal Mucosa/parasitology , Mice , Mice, SCID , Parasite Load , RNA, Messenger/agonists , RNA, Messenger/genetics , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Recombinant Proteins/pharmacology , Signal Transduction , alpha-Defensins/agonists , alpha-Defensins/genetics , alpha-Defensins/immunology , beta-Defensins/agonists , beta-Defensins/genetics , beta-Defensins/immunology
3.
Proc Natl Acad Sci U S A ; 111(52): 18703-8, 2014 Dec 30.
Article in English | MEDLINE | ID: mdl-25512526

ABSTRACT

The pulmonary airways are continuously exposed to bacteria. As a first line of defense against infection, the airway surface liquid (ASL) contains a complex mixture of antimicrobial factors that kill inhaled and aspirated bacteria. The composition of ASL is critical for antimicrobial effectiveness. For example, in cystic fibrosis an abnormally acidic ASL inhibits antimicrobial activity. Here, we tested the effect of pH on the activity of an ASL defensin, human ß-defensin-3 (hBD-3), and the cathelicidin-related peptide, LL-37. We found that reducing pH from 8.0 to 6.8 reduced the ability of both peptides to kill Staphylococcus aureus. An acidic pH also attenuated LL-37 killing of Pseudomonas aeruginosa. In addition, we discovered synergism between hBD-3 and LL-37 in killing S. aureus. LL-37 and lysozyme were also synergistic. Importantly, an acidic pH reduced the synergistic effects of combinations of ASL antibacterials. These results indicate that an acidic pH reduces the activity of individual ASL antimicrobials, impairs synergism between them, and thus may disrupt an important airway host defense mechanism.


Subject(s)
Anti-Infective Agents/pharmacology , Antimicrobial Cationic Peptides/pharmacology , Pseudomonas aeruginosa/growth & development , Staphylococcus aureus/growth & development , beta-Defensins/pharmacology , Antimicrobial Cationic Peptides/agonists , Drug Synergism , Humans , Hydrogen-Ion Concentration , beta-Defensins/agonists , Cathelicidins
4.
Reprod Biol Endocrinol ; 12: 76, 2014 Aug 07.
Article in English | MEDLINE | ID: mdl-25099571

ABSTRACT

BACKGROUND: The majority of beta-defensin family members are exclusively expressed in the epididymis, and some members have been shown to play essential roles in sperm maturation and fertility in rats, mice and humans. Therefore, beta-defensins are hypothesized to be potential targets for contraception and infertility diagnosis and treatment. Clarifying the regulatory mechanisms for the expression of these genes is necessary. Androgen/androgen receptor (AR) signaling plays an important regulatory role in epididymal structure and function. However, very little is known about the androgenic regulation on the production and secretion of the epididymal beta-defensins. METHODS: The expression of beta-defensins was detected by quantitative RT-PCR. The androgen dependence of beta-defensins was determined by bilateral orchiectomy and androgen supplementation. The androgen response elements (AREs) in the promoters of beta-defensins were identified using the MatInspector software. The binding of AR to AREs was assayed by ChIP-PCR/qPCR. RESULTS: We demonstrated that 23 mouse caput epididymal beta-defensins were differentially regulated by androgen/androgen receptor. Six genes, Defb18, 19, 20, 39, 41, and 42, showed full regulation by androgens. Ten genes, Defb15, 30, 34, 37, 40, 45, 51, 52, 22 and Spag11a, were partially regulated by androgens. Defb15, 18, 19, 20, 30, 34, 37, 39, 41, 42, 22 and Spag11a were associated with androgen receptor binding sites in their promoter or intronic regions, indicating direct regulation of AR. Six genes, Defb1, 12, 13, 29, 35, and spag11b/c, exhibited an androgen-independent expression pattern. One gene, Defb25, was highly dependent on testicular factors rather on androgens. CONCLUSIONS: The present study provides novel insights into the mechanisms of androgen regulation on epididymal beta-defensins, enabling a better understanding of the function of beta-defensins in sperm maturation and fertility.


Subject(s)
Androgens/pharmacology , Epididymis/drug effects , Gene Expression Regulation, Developmental/drug effects , Receptors, Androgen/metabolism , Response Elements/drug effects , Signal Transduction/drug effects , beta-Defensins/metabolism , Androgens/administration & dosage , Androgens/chemistry , Androgens/metabolism , Animals , Binding Sites , Castration , Chromatin Immunoprecipitation , Computational Biology , Epididymis/metabolism , Injections, Intraperitoneal , Introns/drug effects , Male , Mice, Inbred C57BL , Promoter Regions, Genetic/drug effects , Protein Isoforms/agonists , Protein Isoforms/antagonists & inhibitors , Protein Isoforms/genetics , Protein Isoforms/metabolism , Receptors, Androgen/chemistry , Spermatogenesis/drug effects , Testosterone Propionate/administration & dosage , Testosterone Propionate/chemistry , Testosterone Propionate/metabolism , Testosterone Propionate/pharmacology , beta-Defensins/agonists , beta-Defensins/antagonists & inhibitors , beta-Defensins/genetics
5.
Pigment Cell Melanoma Res ; 25(3): 370-4, 2012 May.
Article in English | MEDLINE | ID: mdl-22364200

ABSTRACT

Binding of melanocortin peptide agonists to the melanocortin-1 receptor of melanocytes results in eumelanin production, whereas binding of the agouti signalling protein inverse agonist results in pheomelanin synthesis. Recently, a novel melanocortin-1 receptor ligand was reported. A ß-defensin gene mutation was found to be responsible for black coat colour in domestic dogs. Notably, the human equivalent, ß-defensin 3, was found to bind with high affinity to the melanocortin-1 receptor; however, the action of ß-defensin as an agonist or antagonist was unknown. Here, we use in vitro assays to show that ß-defensin 3 is able to act as a weak partial agonist for cAMP signalling in human embryonic kidney (HEK) cells expressing human melanocortin-1 receptor. ß-defensin 3 is also able to activate MAPK signalling in HEK cells stably expressing either wild type or variant melanocortin-1 receptors. We suggest that ß-defensin 3 may be a novel melanocortin-1 receptor agonist involved in regulating melanocyte responses in humans.


Subject(s)
Receptor, Melanocortin, Type 1/agonists , Signal Transduction/drug effects , alpha-MSH/analogs & derivatives , beta-Defensins/pharmacology , Agouti Signaling Protein/pharmacology , Anticarcinogenic Agents/pharmacology , Cyclic AMP/metabolism , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , HEK293 Cells , Humans , Ligands , MAP Kinase Signaling System/drug effects , Melanocytes/drug effects , Melanocytes/metabolism , Melanocytes/physiology , Receptor, Melanocortin, Type 1/metabolism , Up-Regulation/drug effects , alpha-MSH/pharmacology , beta-Defensins/agonists , beta-Defensins/metabolism
6.
Mol Immunol ; 46(15): 3151-6, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19665233

ABSTRACT

Beta defensins (BD) are cysteine rich, cationic antimicrobial peptides (AMP) produced mainly by epithelial and myeloid cells such as neutrophils. In birds, the neutrophil equivalent heterophils produce avian beta defensins (AvBD) of which AvBD2 is the major isoform. Heterophils recognize pathogens or their derived products through a series of pattern recognition receptors called toll-like receptors (TLR) leading to their antimicrobial activities. This work is the first report of TLR modulation of AvBD2 expression in chickens. To measure the effect of TLR activation on AvBD2 production, the heterophils were cultured with different TLR agonists for 6h. Modulation of AvBD2 levels by TLR activation was measured using direct MALDI mass spectrometry without stable isotopic labeling or chromatographic separation. Chemical modification of the conditioned media was performed using reduction/alkylation with dithiothreitol/iodoacetamide to distinguish TLR treated AvBD2 (reduced/alkylated) from controls (non-reduced). Changes in corrected ion intensity ratios were assumed to reflect AvBD2 modulation in heterophils upon activation with different TLR agonists. In general, TLR agonists increased AvBD2 production with LPS showing the greatest induction and CpG-ODN showing little or no effect. These data show that the direct MALDI-MS coupled with reduction/alkylation may provide a rapid relative quantitative approach to the measurement of agonist-induced differential expression of AvBD2.


Subject(s)
Chickens/immunology , Neutrophils/immunology , Toll-Like Receptors/metabolism , beta-Defensins/biosynthesis , Adjuvants, Immunologic/pharmacology , Animals , Culture Media, Conditioned/chemistry , Ligands , Lipopolysaccharides/pharmacology , Neutrophils/drug effects , Neutrophils/metabolism , Oligodeoxyribonucleotides/pharmacology , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Toll-Like Receptors/agonists , Toll-Like Receptors/immunology , beta-Defensins/agonists , beta-Defensins/immunology
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