RESUMEN
Chrysomya albiceps (Wiedemann) is an important vector of animal and diseases as well as causer of secondary myiasis. In order to minimize the effect of the problems caused by C. albiceps, the pathogenicity of the Beauveria bassiana (Balsamo) Vuillemin (URM 3447), in vitro, over eggs, larvae and adults in acclimatized laboratorial conditions under the temperature of 28 ± 1 C was evaluated. Suspensions of conidia (104, 105, 106, 107 and 108 conidia.mL-1) were done, for the analysis of the biological parameters. In the bioassay with eggs, the percentage of eclosion varied from 84.81 to 97.22%. In the bioassay with larvae, pre-pupa period varied from 1 to 1.04 and of pupa, from 5 to 5.09 days; the percentage of adult emergency from treated larvae varied from 30.88 to 80.82%. As to the bioassay with adults, the longevity for male and female was of 4.51 to 6.77 and 10.19 to 14.67 days, respectively; the accumulated mortality death on the 7th day varied from 47.50 to 81.20% for males and of 23.70 to 55.00% for females; the period of posture varied from 10 to17.75 days and the percentage of larvae appeared from females infected varied from 71.39 to 91.25%. KEY WORDS: Biological parameters, entomopathogenic fungus, pathogenicity.
Chrysomya albiceps (Wiedemann) é um importante vetor de doenças de animais e humanos, e também causador de miíases secundárias. Avaliou-se a patogenicidade de Beauveria bassiana (Balsamo) Vuillemin (URM 3447), in vitro, sobre ovos, larvas e adultos em condições de laboratório climatizado sob a temperatura de 28 ± 1 C. Foram feitas suspensões de conídios (104, 105, 106, 107 e 108 conídios.mL-1), para análise dos parâmetros biológicos. No bioensaio com ovos, o percentual de eclosão variou de 84,81% a 97,22%. No bioensaio com larvas, o período de pré-pupa variou de 1 a 1,04 e de pupa, de 5,00 a 5,09 dias; o percentual de emergência de adultos a partir de larvas tratadas variou de 30,88% a 80,82%. No bioensaio com adultos, a longevidade para machos e fêmeas foi de 4,51 a 6,77 e 10,19 a 14,67 dias, respectivamente; a mortalidade acumulada no 7 dia variou de 47,50 % a 81,20% para machos e de 23,70 % a 55,00% dias para fêmeas; o período de postura variou de 10 a 17,75 dias e o percentual de larvas eclodidas a partir de fêmeas infectadas variou de 71,39% a 91,25%. PALAVRAS-CHAVES: Fungo entomopatogênico, parâmetros biológicos, patogenicidade.
RESUMEN
Chrysomya albiceps (Wiedemann) is an important vector of animal and diseases as well as causer of secondary myiasis. In order to minimize the effect of the problems caused by C. albiceps, the pathogenicity of the Beauveria bassiana (Balsamo) Vuillemin (URM 3447), in vitro, over eggs, larvae and adults in acclimatized laboratorial conditions under the temperature of 28 ± 1 C was evaluated. Suspensions of conidia (104, 105, 106, 107 and 108 conidia.mL-1) were done, for the analysis of the biological parameters. In the bioassay with eggs, the percentage of eclosion varied from 84.81 to 97.22%. In the bioassay with larvae, pre-pupa period varied from 1 to 1.04 and of pupa, from 5 to 5.09 days; the percentage of adult emergency from treated larvae varied from 30.88 to 80.82%. As to the bioassay with adults, the longevity for male and female was of 4.51 to 6.77 and 10.19 to 14.67 days, respectively; the accumulated mortality death on the 7th day varied from 47.50 to 81.20% for males and of 23.70 to 55.00% for females; the period of posture varied from 10 to17.75 days and the percentage of larvae appeared from females infected varied from 71.39 to 91.25%. KEY WORDS: Biological parameters, entomopathogenic fungus, pathogenicity.
Chrysomya albiceps (Wiedemann) é um importante vetor de doenças de animais e humanos, e também causador de miíases secundárias. Avaliou-se a patogenicidade de Beauveria bassiana (Balsamo) Vuillemin (URM 3447), in vitro, sobre ovos, larvas e adultos em condições de laboratório climatizado sob a temperatura de 28 ± 1 C. Foram feitas suspensões de conídios (104, 105, 106, 107 e 108 conídios.mL-1), para análise dos parâmetros biológicos. No bioensaio com ovos, o percentual de eclosão variou de 84,81% a 97,22%. No bioensaio com larvas, o período de pré-pupa variou de 1 a 1,04 e de pupa, de 5,00 a 5,09 dias; o percentual de emergência de adultos a partir de larvas tratadas variou de 30,88% a 80,82%. No bioensaio com adultos, a longevidade para machos e fêmeas foi de 4,51 a 6,77 e 10,19 a 14,67 dias, respectivamente; a mortalidade acumulada no 7 dia variou de 47,50 % a 81,20% para machos e de 23,70 % a 55,00% dias para fêmeas; o período de postura variou de 10 a 17,75 dias e o percentual de larvas eclodidas a partir de fêmeas infectadas variou de 71,39% a 91,25%. PALAVRAS-CHAVES: Fungo entomopatogênico, parâmetros biológicos, patogenicidade.