Halophilic soil microbial strains improve the moisture stress tolerance in oilseed crop by sustaining Photosystem II functionality.

The sunflower (Helianthus annus L.) is a vital oilseed crop exposed to drought globally. A vast proportion of research is devoted to the naturally occurring microbes and their interaction with plants to alleviate stress consequences. Halophilic bacterial strains, i.e., Bacillus cereus KUB-15 (accession number NR 074540.1), KUB-27 (accession number NR 074540.1), and Bacillus licheniformis strain AAB9 (accession number MW362506), were isolated. Later, isolated strains were used for sunflower through inoculation. Plants were allowed to grow, and thirty-days-old plants were exposed to fixed moisture stress (40-45%). The functionality of photosystem II, light-harvesting ability, and physiological tolerance of cultivars were examined. Bacterial strains B. licheniformis sustained substantial electron flow in between photosystem II (PS II) and photosystem I (PS 1) that not only favored the passable photosynthetic performance but also enhanced antioxidant enzyme activity under stress condition. Compared to other halophilic strains, Bacillus licheniformis did manage reasonable relative water content (RWC), chlorophyll content index (CCI) and biomass production under stress condition. In comparison to both sunflower cultivars, bacterial inoculation was greatly restored growth and photosynthetic performance in Agsun-5264 than S-278 under moisture stress environment. Hence, it is suggested that that bacterial strain and plants cultivar compatibility are essential aspect for sustainable agriculture production.

Scrutinizes the Sustainable Role of Halophilic Microbial Strains on Oxygen-Evolving Complex, Specific Energy Fluxes, Energy Flow and Nitrogen Assimilation of Sunflower Cultivars in a Suboptimal Environment.

Environmental extremes such as hypersaline conditions are significant threats to agricultural productivity. The sustainable use of halophilic microbial strains was evaluated in plant in a salt stress environment. Oxygen-evolving complex (OEC), energy compartmentalization, harvesting efficiencies (LHE), specific energy fluxes (SEF), and nitrogen assimilation of oilseed crops (Sunflower cultivars) in a suboptimal environment was examined. Plants were grown in a plastic pot (15 ×18 cm2) containing sterilized (autoclaved at 120°C for 1 h) soil. Twenty-five ml suspension (107 CFU/ml) each of Bacillus cereus strain KUB-15 and KUB-27 (accession number NR 074540.1) and Bacillus licheniformis strain AAB9 (accession number MW362506), were applied via drenching method. Month-old plants were subjected to salt stress via gradual increment method. The energy compartmentalization of microbial inoculated plants exposed to salt stress revealed higher photosystem II (PSII) activity at the donor side, lesser photo-inhibition, and increased performance of oxygen-evolving complex compared to control. High potassium (K+) and low sodium (Na+) ions in treated leaves with the activated barricade of the antioxidant system stimulated by Bacillus strains favored enhanced photochemical efficiency, smooth electron transport, and lesser energy dissipation in the stressed plants. Moreover, the results reveal the increased activity of nitrite reductase (NiR) and nitrate reductase (NR) by microbial inoculation that elevated the nitrogen availability in the salt-stressed plant. The current research concludes that the application of bio-inoculants that reside in the hyper-saline environment offers substantial potential to enhance salt tolerance in sunflowers by modulating their water uptake, chlorophyll, nitrogen metabolism, and better photochemical yield.

Synthesis of metal anthranilate complexes: catalytic and antipathogenic studies.

BACKGROUND: Anthranilic acid is an active compound with diverse biological activities such as anti-inflammatory, antineoplastic, anti-malarial and α-glucosidase inhibitory properties. It can also chelate transition metals to form complexes with applications as antipathogens, photoluminescent materials, corrosion inhibitors, and catalysts. RESULTS: Anthranilic acid complexes (1-10) of Zn(II), Bi(III), Ag(I), Fe(II), Co(II), Cu(II), Mn(II), Al, Ni(II), and Cr(III) were synthesized and characterized using thermogravimetric (TGA), elemental analysis, FT-IR, UV-vis spectrometry, mass spectrometry and magnetic susceptibility. The morphology and size of metal complex (1-10) particles were determined by scanning electron microscope (SEM) and the surface area was determined by BET analysis. TGA and CHN analysis data indicated that the stoichiometries of complexes were 1:2 metal/ligand except for Ag(I), Al and Bi. Furthermore, DFT study was performed to optimize the structure of selected complexes. The complexes (1-10) were evaluated for their catalytic activity in the reduction of 4-nitrophenol (4-NP), antibacterial activity against S. aureus, P. aeroginosa and E. coli as well as their antifungal activity against F. solani and A. niger. The complexes were also tested against the second-stage juveniles (J2) root-knot nematodes. CONCLUSION: Co(II) complex 5 and Cu(II) complex 6 showed high catalytic activity for the reduction of 4-NP to 4-aminophenol (4-AP). Ag(I) complex 3 showed the best activity against the pathogens that were tested namely clinically important bacteria S. aureus, P. aeroginosa and E. coli, commercially important fungi F. solani and A. niger and J2 root-knot nematodes M. javanica.

https://www.fungiofpakistan.com: a continuously updated online database of fungi in Pakistan.

The website fungiofpakistan.com is a collection of all the available data about macro- as well as micro-fungi collected from Pakistan. This website comprises reported fungal species with isolation source or host record, locality and updated classification. The data on this website is based on old literature (library data, personal data of specific authors or books that were not easily accessible to public) and recent publications. This website is an important potential platform for researchers, government officials, industries and other users. Users can provide their inputs related to missing taxa, new genera, the new record and new data. They also have the opportunity to express their opinions on valid names, invalid names and illegitimate names, with notes published in the 'Notes' section of webpage provided following review and editing by curators and fungal taxonomists. This website plays a significant contribution to our knowledge of the rich fungal diversity of Pakistan. However, much more sustained and detailed research is needed to fully evaluate fungal diversity in Pakistan. Undoubtedly, that many more fungi will be discovered and added in the future. DATABASE URL: https://fungiofpakistan.com/.

Synthesis, characterization, anti-bacterial, anti-fungal and nematicidal activities of 2-amino-3-cyanochromenes.

Soil-borne plant pathogens such as nematodes, fungi and some bacteria not only affect the plant cultures but also economy and environmental implications. As a result of these pathogens attacks, the yield and quality of crops is affected. Therefore, synthesis of new compounds to control these pathogens is very important and need of time. Chromenes are considered important group of heterocyclic compounds and exhibited significant biological activities. 2-amino-3-cyano chromenes are considered important medicinal scaffolds among the chromenes. We describe three component microwave assisted synthesis of 2-amino-3-cyano chromenes. The versatility of the reaction was examined by varying the aldehydes in the reaction mixture which lead to the synthesis of series of 2-amino-3-cyanochromenes (1-9). The structural elucidations of the compounds were studied by 1H NMR, 13C NMR, HRMS and FTIR spectrometer and results were well correlated. The synthesized compounds were evaluated for their anti-bacterial, anti-fungal and nematicidal activities. Among the synthesized compounds, compound 6 showed good anti-bacterial activity while compound 9 exhibited high anti-fungal activity. In case of nematicidal activity, compound 8 indicated high activity as compare with other compounds followed by compound 9. Results indicate that these synthesized compounds could be used as effective control for soil-borne plant pathogens such as nematodes, fungi and bacteria and can help to improve the plant production.

Synthesis, spectral studies and biological evaluation of 2-aminonicotinic acid metal complexes.

We synthesized 2-aminonicotinic acid (2-ANA) complexes with metals such as Co(II), Fe(III), Ni(II), Mn(II), Zn(II), Ag(I),Cr(III), Cd(II) and Cu(II) in aqueous media. The complexes were characterized and elucidated using FT-IR, UV-Vis, a fluorescence spectrophotometer and thermo gravimetric analysis (TGA). TGA data showed that the stoichiometry of complexes was 1:2 metal/ligand except for Ag(I) and Mn(II) where the ratio was 1:1. The metal complexes showed varied antibacterial, fungicidal and nematicidal activities. The silver and zinc complexes showed highest activity against Bacillus subtilis and Bacillus licheniformis respectively. Fusarium oxysporum was highly susceptible to nickel and copper complexes whereas Macrophomina phaseolina was completely inert to the complexes. The silver and cadmium complexes were effective against the root-knot nematode Meloidogyne javanica.