Hydroxyapatite nano-pillars on TI-6Al-4V: Enhancements in cell spreading and proliferation during cell/surface integration.

Despite the attractive combinations of cell/surface interactions, biocompatibility, and good mechanical properties of Ti-6Al-4V, there is still a need to enhance the early stages of cell/surface integration that are associated with the implantation of biomedical devices into the human body. This paper presents a novel, easy and reproducible method of nanoscale and nanostructured hydroxyapatite (HA) coatings on Ti-6Al-4V. The resulting nanoscale coatings/nanostructures are characterized using a combination of Raman spectroscopy, scanning electron microscopy equipped with energy dispersive x-ray spectroscopy. The nanostructured/nanoscale coatings are shown to enhance the early stages of cell spreading and integration of bone cells (hFOB cells) on Ti-6Al-4V surfaces. The improvements include the acceleration of extra-cellular matrix, cell spreading and proliferation by nanoscale HA structures on the coated surfaces. The implications of the results are discussed for the development of HA nanostructures for the improved osseointegration of Ti-6Al-4V in orthopedic and dental applications.

Multicomponent Photocatalytic-Dispersant System for Oil Spill Remediation.

In the present work, the potential application of a fabricated halloysite nanotubes-Ag-TiO2 (HNT-Ag-TiO2) composite loaded with a binary surfactant mixture made up of lecithin and Tween 80 (LT80) in remediating oil spillages was examined. The as-prepared Ag-TiO2 that was used in the fabrication of the HNT-Ag-TiO2-LT80 composite was characterized by X-ray diffraction, Raman spectroscopy, UV-vis and diffuse reflectance spectroscopy, CV analyses, and SEM-EDX. The synthesized composite was also characterized by thermogravimetric analysis, Fourier-transform infrared spectroscopy, and scanning electron microscopy-energy dispersive X-ray spectroscopy. The synthesized composite was active in both the UV and visible light regions of the electromagnetic spectrum. The oil-remediating potential of the as-prepared composite was examined on crude oil, and aromatics and asphaltene fractions of crude oil. The composite was able to reduce the surface tension, form stable emulsions and smaller oil droplet sizes, and achieve a high dispersion effectiveness of 91.5%. A mixture of each of the crude oil and its fractions and HNT-Ag-TiO2-LT80 was subjected to photodegradation under UV light irradiation. The results from the GC-MS and UV-vis analysis of the photodegraded crude oil revealed that the photocatal composite was able to photodegrade the crude oil, aromatics, and asphaltene fractions of crude oil with the formation of intermediate photodegradation products depicting that the HNT-Ag-TiO2-LT80 has a potential as an oil spill remediation material.

Chicken Feather Protein Dispersant for Effective Crude Oil Dispersion in the Marine Environment.

Various studies report that aside from the adverse impact of the crude oil on the marine environment, there is the likelihood that chemical dispersants used on the surface of water as oil-treating agents themselves possess a degree of toxicity, which have additional effects on the environment. To eliminate the subject of toxicity, there exist several materials in nature that have the ability to form good emulsions, and such products include protein molecules. In this study, chicken feathers which are known to contain ≥90% protein were used to formulate a novel dispersant to disperse crude oil in seawater (35 ppt). Protein from chicken feathers was extracted and synthesized into the chicken feather protein (CFP) dispersant using deionized water as a solvent. Emulsions formed from CFP-synthesized dispersants were stable over a considerably long period of time, whereas the droplet sizes of the emulsion formed were on the average very small in diameter, making droplet coalescence very slow. The CFP dispersants exhibited moderate surface and interfacial activity at normal seawater salinity. Using the US EPA's baffled flask test, at 800 and 1000 mg/ml CFP surfactant-to-oil ratios, dispersion effectiveness values of 56.92 and 68.64 vol % were obtained, respectively, which show that CFP has a great potential in crude oil dispersion. Moreover, the acute toxicity test performed on Nile tilapia showed that CFP was practically nontoxic with an LC50 value of more than 100 mg/L after 96 h of exposure. The results obtained showed that the CFP dispersant is environmentally friendly.

Managing Excess Lead Iodide with Functionalized Oxo-Graphene Nanosheets for Stable Perovskite Solar Cells.

Stability issues could prevent lead halide perovskite solar cells (PSCs) from commercialization despite it having a comparable power conversion efficiency (PCE) to silicon solar cells. Overcoming drawbacks affecting their long-term stability is gaining incremental importance. Excess lead iodide (PbI2 ) causes perovskite degradation, although it aids in crystal growth and defect passivation. Herein, we synthesized functionalized oxo-graphene nanosheets (Dec-oxoG NSs) to effectively manage the excess PbI2 . Dec-oxoG NSs provide anchoring sites to bind the excess PbI2 and passivate perovskite grain boundaries, thereby reducing charge recombination loss and significantly boosting the extraction of free electrons. The inclusion of Dec-oxoG NSs leads to a PCE of 23.7 % in inverted (p-i-n) PSCs. The devices retain 93.8 % of their initial efficiency after 1,000 hours of tracking at maximum power points under continuous one-sun illumination and exhibit high stability under thermal and ambient conditions.

Curcumin loaded Ag-TiO2-halloysite nanotubes platform for combined chemo-photodynamic therapy treatment of cancer cells.

The use of naturally occurring anticancer materials in combination with doped metal oxide has emerged as one of the most promising ways for improving anticancer treatment efficacy. In this study, the anticancer potential of curcumin-loaded Ag-TiO2-halloysite nanotubes (curcumin-loaded Ag-TiO2-HNTs) was examined. Ag-TiO2-HNTs with different wt% of Ag-TiO2 were synthesized and characterized using XRD, TGA, FT-IR, UV-Vis spectroscopy, and SEM-EDX. The XRD results revealed the presence of crystalline TiO2. However, the presence of Ag was detected through the SEM-EDX analysis. Cyclic voltammetry measurements suggested the enhancement of the release of ROS from TiO2 upon deposition with Ag. FT-IR and TGA analysis confirmed the successful loading of curcumin inside the nanotubes of the halloysite. In vitro drug released studies revealed the release of approximately 80-99% curcumin within 48 hours. Kinetic model studies revealed that the release of curcumin from HNT and Ag-TiO2-HNT followed the first-order and Higuchi models, respectively. The light irradiated curcumin-loaded Ag-TiO2-HNTs samples exhibited considerable anticancer potential as compared to the free curcumin, irradiated Ag-TiO2 NPs samples, and unirradiated curcumin loaded Ag-TiO2-HNTs samples. The obtained results revealed that combined chemo- and photodynamic therapy using curcumin-loaded Ag-TiO2-HNTs nanomaterial has the potential as an effective anticancer treatment method.

Dispersants as an oil spill clean-up technique in the marine environment: A review.

Oil is a major source of energy in the industrial world. Exploitation of oil and rigging activities, transportation via sea, and many other mechanical failures lead to oil spills into the marine environment. In view of these, the suitability and effectiveness of oil spill response methods have always been a topical discussion worldwide. It has become necessary, now than ever, for existing spill response methods used to remove oil from the environment to be improved upon and more importantly, develop new response materials that are sustainable and environmentally friendly. There exist surfactants in nature that are non-toxic and biodegradable, which can be explored to produce potential dispersants to help remove oil safely from the surface of marine water. This review comprises of the works and resourceful materials produced by various researchers and agencies in the field of oil spill response, placing emphasis on the use of dispersants in the marine environment.

Chitosan-Coated Halloysite Nanotubes As Vehicle for Controlled Drug Delivery to MCF-7 Cancer Cells In Vitro.

The aim of the work is to improve the release properties of curcumin onto human breast cancer cell lines using coated halloysite nanotubes (HNTs) with chitosan as a polycation. A loading efficiency of 70.2% (w/w) was attained for loading 4.9 mg of the drug into 0.204 g bed volume of HNTs using the vacuum suction method. Results acquired from Brunauer-Emmett-Teller (BET), Fourier-transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), scanning electron spectroscopy (SEM), zeta potential, and thermogravimetric analysis (TGA) indicated the presence of the drug and the biopolymer in and around the nanotubes. The release properties of drug-loaded HNTs (DLHNTs) and chitosan-coated drug-loaded HNTs (DLHNTs-CH) were evaluated. The release percentages of DLHNTs and DLHNTs-CH after 6 h were 50.7 and 37%, respectively. Based on the correlation coefficients obtained by fitting the release nature of curcumin from the two samples, the Korsmeyer-Peppas model was found to be the best-fitted model. In vitro cell viability studies were carried out on the human breast cancer cell line MCF-7, using the MTT and trypan blue exclusion assays. Prior to the Trypan blue assay, the IC50 of curcumin was determined to be ~30 µM. After 24 h of incubation, the recorded cell viability values were 94, 68, 57, and 51% for HNTs, DLHNTs-CH, DLHNTs, and curcumin, respectively. In comparison to the release studies, it could be deducted that sustained lethal doses of curcumin were released from the DLHNTs-CH within the same time. It is concluded from this work that the "burst release" of naked drugs could be slowly administered using chitosan-coated HNTs as potential drug carriers.

Synthesis of TiO2-Ag3PO4 photocatalyst material with high adsorption capacity and photocatalytic activity: application in the removal of dyes and pesticides.

The photocatalytic activity of TiO2 can be enhanced by coupling it with other semiconductors and the semiconductor composites may find useful application in water treatment technologies. TiO2-Ag3PO4 composites were synthesized and characterized with XRD, SEM-EDX and DRS. The synthesized TiO2-Ag3PO4 showed high photocatalytic activity in the presence UV-vis light on rhodamine B, methylene blue and the pesticides imidacloprid, atrazine and pyrimethanil. LC-MS analysis of the photodegraded pyrimethanil led to the identification of hydroxylated and aliphatic derivatives of pyrimethanil. The photocatalytic activity of the coupled semiconductor was higher than that of the bare TiO2 and Ag3PO4 and this was attributed to the unique band matching between TiO2 and Ag3PO4 which resulted in efficient charge separation and subsequent reduction in the electron-hole recombination. In addition, the synthesized TiO2-Ag3PO4 showed strong adsorption for water soluble dyes implying that TiO2-Ag3PO4 can remove pollutants through photocatalysis and adsorption. The results from the study showed the potential application of TiO2-Ag3PO4 composite in water treatment technologies.

Ag2CO3-halloysite nanotubes composite with enhanced removal efficiency for water soluble dyes.

The release of water soluble dyes into the environment is an utmost concern in many countries. This paper presents the effects of Ag2CO3-halloysite composites on the efficient removal of water soluble dyes. In this study, NaHCO3 solution was added dropwisely to halloysite nanotubes (HNTs) dispersed in aqueous AgNO3 to form Ag2CO3-HNTs composite. The synthesized Ag2CO3-HNTs composite was characterized with Diffused Reflectance Spectroscopy (DRS), X-ray Diffraction (XRD), Thermogravimetric analysis (TGA), Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDX) and Fourier Transform Infra-Red (FT-IR) spectroscopy. The photocatalytic activity and the adsorption capacity of Ag2CO3-HNTs on methylene blue and rhodamine b dyes were dependent on pH and the amount of HNTs used in the synthesis. The photodegradation efficiency of Ag2CO3 was lower when compared with that of the composite material. This observation is due to the reduction in the electron-hole recombination with the HNTs acting as electron trapping site and the enhanced aqueous dispersity of Ag2CO3-HNTs. The enhanced adsorption of water soluble dyes by the Ag2CO3-HNTs resulted from the electrostatic attraction of cationic dyes to the surface of the HNTs (negatively charged). The Ag2CO3-HNTs therefore removed dye pollutants through a combination of photocatalytic and adsorption processes. The results obtained during the study confirmed the potential application of Ag2CO3-HNTs composite in water treatment technologies.

Modified halloysite nanoclay as a vehicle for sustained drug delivery.

This paper presents the effect of modified halloysite nanotubes on the sustained drug release mechanisms of sodium salicylate. Acid treatment and composite polymer-halloysite modification techniques were adopted in this study. After each modification, sodium salicylate drug was loaded, and in vitro release properties were evaluated and compared with the raw unmodified halloysite nanotubes. The results obtained from SEM, TEM and FTIR analyses indicate that both acid treatment and composite formation have no effect on the tubular structure and morphology of halloysite. However, modification of the halloysite nanotubes did influence the drug release rate. In the acid treatment modification, there was an improved loading of sodium salicylate drug which resulted in the sustain release of large amount of the sodium salicylate. In the polymer/halloysite composite formation, a consistent layer of polymer was formed around the halloysite during the composite formation and thus delayed release providing sustained release of sodium salicylate drug over a longer period of time as compared to the acid treated and unmodified halloysite. The results from the invitro release were best fitted with the Higuchi and the Koresymer-Peppas models.